Izalontamab / 5f88

A4DAB149_5f88_D_C | Source PDB: 5f88 | Chains: D, C | Done
99.56
Yes
10 Å
Yes
Avg A4D Score
-0.6308
Max Score
2.1515
Min Score
-3.7096
Total Score
-268.73
Score CDR
-0.2619
Score non-CDR
-0.6755

Calculation Parameters

Chain Sequence(s) QVQLKQSGPGLVQPSQSLSITCTVSGFSLTNYGVHWVRQSPGKGLEWLGVIWSGGNTDYNTPFTSRLSINKDNSKSQVFFKMNSLQSNDTAIYYCARALTYYDYEFAYWGQGTLVTVSAASTKGPSVFPLAPSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKDILLTQSPVILSVSPGERVSFSCRASQSIGTNIHWYQQRTNGSPRLLIKYASESISGIPSRFSGSGSGTDFTLSINSVESEDIADYYCQQNNNWPTTFGAGTKLELKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGA input.pdb
Selected Chain(s) D (Heavy), C (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.7096
Maximum Score 2.1515
Average Score -0.6308
Total Score -268.7269

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q D -1.3178 Soluble
2 V D -0.5694 Soluble
3 Q D -1.2003 Soluble
4 L D 0.0000 Buried
5 K D -2.0360 Soluble
6 Q D 0.0000 Buried
7 S D -0.8331 Soluble
8 G D -1.1042 Soluble
9 P D -0.4544 Soluble
11 G D -0.3719 Soluble
12 L D 0.0586 Aggregation-prone
13 V D -0.5609 Soluble
14 Q D -1.1023 Soluble
15 P D -1.4234 Soluble
16 S D -1.5059 Soluble
17 Q D -1.8903 Soluble
18 S D -1.7859 Soluble
19 L D 0.0000 Buried
20 S D -0.8627 Soluble
21 I D 0.0000 Buried
22 T D -0.5375 Soluble
23 C D 0.0000 Buried
24 T D -1.0228 Soluble
25 V D 0.0000 Buried
26 S D -1.0145 Soluble
27 G D -0.8307 Soluble
28 F D -0.4585 Soluble
29 S D -0.8239 Soluble
30 L D 0.0000 Buried
35 T D -0.8748 Soluble
36 N D -0.8197 Soluble
37 Y D 0.4721 Aggregation-prone
38 G D 0.0000 Buried
39 V D 0.0000 Buried
40 H D 0.0000 Buried
41 W D 0.0000 Buried
42 V D 0.0000 Buried
43 R D 0.0000 Buried
44 Q D -0.5205 Soluble
45 S D -1.0326 Soluble
46 P D -1.2181 Soluble
47 G D -1.7671 Soluble
48 K D -2.2357 Soluble
49 G D -1.1598 Soluble
50 L D 0.0000 Buried
51 E D 0.0000 Buried
52 W D 0.0000 Buried
53 L D 0.0000 Buried
54 G D 0.0000 Buried
55 V D 0.0000 Buried
56 I D 0.0000 Buried
57 W D -0.5060 Soluble
58 S D -0.6036 Soluble
59 G D -1.2119 Soluble
63 G D -1.5154 Soluble
64 N D -1.8690 Soluble
65 T D -1.3503 Soluble
66 D D -1.0894 Soluble
67 Y D -0.3972 Soluble
68 N D 0.0000 Buried
69 T D -0.1899 Soluble
70 P D -0.3628 Soluble
71 F D 0.0000 Buried
72 T D -0.4689 Soluble
74 S D -0.6553 Soluble
75 R D -1.2877 Soluble
76 L D 0.0000 Buried
77 S D -1.2899 Soluble
78 I D 0.0000 Buried
79 N D -1.7619 Soluble
80 K D -1.3739 Soluble
81 D D -1.7109 Soluble
82 N D -2.1492 Soluble
83 S D -1.6541 Soluble
84 K D -2.3353 Soluble
85 S D -1.5706 Soluble
86 Q D -1.4412 Soluble
87 V D 0.0000 Buried
88 F D -0.2938 Soluble
89 F D 0.0000 Buried
90 K D -2.0761 Soluble
91 M D 0.0000 Buried
92 N D -2.3292 Soluble
93 S D -1.6063 Soluble
94 L D 0.0000 Buried
95 Q D -2.0046 Soluble
96 S D -1.4269 Soluble
97 N D -2.0450 Soluble
98 D D 0.0000 Buried
99 T D -0.6076 Soluble
100 A D 0.0000 Buried
101 I D 0.3424 Aggregation-prone
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 A D 0.0000 Buried
106 R D 0.0000 Buried
107 A D 0.0000 Buried
108 L D 1.0236 Aggregation-prone
109 T D 1.1139 Aggregation-prone
110 Y D 1.8185 Aggregation-prone
111 Y D 1.8913 Aggregation-prone
112 D D 0.9899 Aggregation-prone
113 Y D 0.5336 Aggregation-prone
114 E D 0.0000 Buried
115 F D 0.0000 Buried
116 A D 0.2365 Aggregation-prone
117 Y D 0.2632 Aggregation-prone
118 W D -0.4207 Soluble
119 G D 0.0000 Buried
120 Q D -1.7675 Soluble
121 G D -0.9393 Soluble
122 T D 0.0000 Buried
123 L D 0.1148 Aggregation-prone
124 V D 0.0000 Buried
125 T D 0.0000 Buried
126 V D 0.0000 Buried
127 S D -0.5283 Soluble
128 A D -0.1989 Soluble
129 A D -0.2291 Soluble
130 S D -0.4392 Soluble
131 T D -0.5529 Soluble
132 K D -1.1672 Soluble
133 G D -1.2714 Soluble
134 P D -0.5737 Soluble
135 S D -0.4080 Soluble
136 V D 0.0000 Buried
137 F D 0.0000 Buried
138 P D 0.0000 Buried
139 L D 0.0000 Buried
140 A D -0.9603 Soluble
141 P D -0.6037 Soluble
142 S D -0.7001 Soluble
143 G D -0.6409 Soluble
144 T D -0.4842 Soluble
145 A D -0.2529 Soluble
146 A D 0.0000 Buried
147 L D 0.0000 Buried
148 G D 0.0000 Buried
149 C D 0.0000 Buried
150 L D 0.0000 Buried
151 V D 0.0000 Buried
152 K D 0.0000 Buried
153 D D -0.4006 Soluble
154 Y D 0.0000 Buried
155 F D 0.0000 Buried
156 P D 0.0000 Buried
157 E D -0.1974 Soluble
158 P D -0.3994 Soluble
159 V D -0.4458 Soluble
160 T D -0.4137 Soluble
161 V D -0.1346 Soluble
162 S D -0.3940 Soluble
163 W D 0.0000 Buried
164 N D -0.8022 Soluble
165 S D -0.6782 Soluble
166 G D -0.4983 Soluble
167 A D -0.2269 Soluble
168 L D 0.0058 Aggregation-prone
169 T D -0.1714 Soluble
170 S D -0.1877 Soluble
171 G D -0.2449 Soluble
172 V D 0.1450 Aggregation-prone
173 H D -0.2863 Soluble
174 T D 0.0501 Aggregation-prone
175 F D 0.0000 Buried
176 P D -0.3086 Soluble
177 A D 0.3389 Aggregation-prone
178 V D 0.5483 Aggregation-prone
179 L D 1.0981 Aggregation-prone
180 Q D 0.2432 Aggregation-prone
181 S D -0.0882 Soluble
182 S D -0.2390 Soluble
183 G D -0.0184 Soluble
184 L D 0.1529 Aggregation-prone
185 Y D 0.4225 Aggregation-prone
186 S D 0.0000 Buried
187 L D 0.0000 Buried
188 S D 0.0000 Buried
189 S D 0.0000 Buried
190 V D 0.0000 Buried
191 V D 0.0000 Buried
192 T D -0.1413 Soluble
193 V D 0.0000 Buried
194 P D -0.5655 Soluble
195 S D -0.5215 Soluble
196 S D -0.5889 Soluble
197 S D -0.5629 Soluble
198 L D -0.6651 Soluble
199 G D -0.8579 Soluble
200 T D -0.6800 Soluble
201 Q D -1.0850 Soluble
202 T D -1.0097 Soluble
203 Y D 0.0000 Buried
204 I D -1.2881 Soluble
205 C D 0.0000 Buried
206 N D -1.4229 Soluble
207 V D 0.0000 Buried
208 N D -1.7095 Soluble
209 H D 0.0000 Buried
210 K D -2.2976 Soluble
211 P D -1.3499 Soluble
212 S D -1.6130 Soluble
213 N D -2.3468 Soluble
214 T D -1.8936 Soluble
215 K D -2.5476 Soluble
216 V D -1.6015 Soluble
217 D D -2.4557 Soluble
218 K D -2.0191 Soluble
219 R D -2.3157 Soluble
220 V D 0.0000 Buried
221 E D -2.0714 Soluble
222 P D -1.5143 Soluble
223 K D -2.0475 Soluble
1 D C -1.2279 Soluble
2 I C -0.2758 Soluble
3 L C 0.7166 Aggregation-prone
4 L C 0.0000 Buried
5 T C -0.3315 Soluble
6 Q C 0.0000 Buried
7 S C 0.2208 Aggregation-prone
8 P C 0.9596 Aggregation-prone
9 V C 2.1515 Aggregation-prone
10 I C 1.8324 Aggregation-prone
11 L C 0.7780 Aggregation-prone
12 S C -0.0646 Soluble
13 V C 0.0000 Buried
14 S C -1.8397 Soluble
15 P C -2.0737 Soluble
16 G C -2.2129 Soluble
17 E C -3.0098 Soluble
18 R C -3.1704 Soluble
19 V C 0.0000 Buried
20 S C -0.9113 Soluble
21 F C 0.0000 Buried
22 S C -0.8574 Soluble
23 C C 0.0000 Buried
24 R C -2.4543 Soluble
25 A C 0.0000 Buried
26 S C -0.9000 Soluble
27 Q C -1.2677 Soluble
28 S C -1.4176 Soluble
29 I C 0.0000 Buried
36 G C -0.9827 Soluble
37 T C -0.7616 Soluble
38 N C -0.7846 Soluble
39 I C 0.0000 Buried
40 H C 0.0000 Buried
41 W C 0.0000 Buried
42 Y C 0.0000 Buried
43 Q C 0.0000 Buried
44 Q C -1.8402 Soluble
45 R C -2.8173 Soluble
46 T C -2.1803 Soluble
47 N C -2.0754 Soluble
48 G C -1.9048 Soluble
49 S C -1.2544 Soluble
50 P C 0.0000 Buried
51 R C -1.1064 Soluble
52 L C 0.0000 Buried
53 L C 0.0000 Buried
54 I C 0.0000 Buried
55 K C -0.7624 Soluble
56 Y C -0.6290 Soluble
57 A C 0.0000 Buried
65 S C -1.2567 Soluble
66 E C -2.0019 Soluble
67 S C -1.1834 Soluble
68 I C -0.5024 Soluble
69 S C -0.3376 Soluble
70 G C -0.4750 Soluble
71 I C -0.2955 Soluble
72 P C -0.3353 Soluble
74 S C -0.5326 Soluble
75 R C -1.0396 Soluble
76 F C 0.0000 Buried
77 S C -0.8111 Soluble
78 G C -0.7423 Soluble
79 S C -0.8576 Soluble
80 G C -1.0767 Soluble
83 S C -0.9370 Soluble
84 G C -1.2886 Soluble
85 T C -1.8506 Soluble
86 D C -2.4963 Soluble
87 F C 0.0000 Buried
88 T C -0.8887 Soluble
89 L C 0.0000 Buried
90 S C -0.8146 Soluble
91 I C 0.0000 Buried
92 N C -2.0358 Soluble
93 S C -2.2099 Soluble
94 V C 0.0000 Buried
95 E C -2.5318 Soluble
96 S C -1.9796 Soluble
98 D C -1.2583 Soluble
99 I C -1.4164 Soluble
100 A C 0.0000 Buried
101 D C -1.2128 Soluble
102 Y C 0.0000 Buried
103 Y C 0.0000 Buried
104 C C 0.0000 Buried
105 Q C 0.0000 Buried
106 Q C 0.0000 Buried
107 N C -0.5819 Soluble
108 N C -1.1329 Soluble
109 N C -0.9859 Soluble
114 W C 0.2337 Aggregation-prone
115 P C 0.0000 Buried
116 T C 0.0000 Buried
117 T C 0.0397 Aggregation-prone
118 F C 0.2264 Aggregation-prone
119 G C 0.0000 Buried
120 A C 0.0235 Aggregation-prone
121 G C 0.1493 Aggregation-prone
122 T C 0.0000 Buried
123 K C -0.0296 Soluble
124 L C 0.0000 Buried
125 E C -0.6410 Soluble
126 L C 0.0000 Buried
127 K C -1.4458 Soluble
128 R C -1.0075 Soluble
129 T C 0.2077 Aggregation-prone
130 V C 1.0619 Aggregation-prone
131 A C 0.3052 Aggregation-prone
132 A C 0.0217 Aggregation-prone
133 P C 0.0000 Buried
134 S C -0.1524 Soluble
135 V C 0.0000 Buried
136 F C 0.1852 Aggregation-prone
137 I C 0.1029 Aggregation-prone
138 F C 0.0000 Buried
139 P C -0.4537 Soluble
140 P C -0.7700 Soluble
141 S C 0.0000 Buried
142 D C -2.8454 Soluble
143 E C -2.8059 Soluble
144 Q C 0.0000 Buried
145 L C -2.2415 Soluble
146 K C -2.7077 Soluble
147 S C -1.6576 Soluble
148 G C -1.1584 Soluble
149 T C -0.8383 Soluble
150 A C 0.0000 Buried
151 S C 0.0000 Buried
152 V C 0.0000 Buried
153 V C 0.0000 Buried
154 C C 0.0000 Buried
155 L C 0.0000 Buried
156 L C 0.0000 Buried
157 N C 0.0000 Buried
158 N C -0.9534 Soluble
159 F C 0.0000 Buried
160 Y C 0.0000 Buried
161 P C -1.3073 Soluble
162 R C -2.2666 Soluble
163 E C -2.8948 Soluble
164 A C -2.1169 Soluble
165 K C -2.1566 Soluble
166 V C -1.0112 Soluble
167 Q C -0.6111 Soluble
168 W C 0.0000 Buried
169 K C -0.6801 Soluble
170 V C 0.0000 Buried
171 D C -1.8939 Soluble
172 N C -1.5575 Soluble
173 A C -0.2950 Soluble
174 L C 0.7184 Aggregation-prone
175 Q C -0.0792 Soluble
176 S C -0.3610 Soluble
177 G C -0.8692 Soluble
178 N C -0.7672 Soluble
179 S C -1.0003 Soluble
180 Q C -1.1766 Soluble
181 E C -1.3724 Soluble
182 S C -0.5646 Soluble
183 V C -0.2057 Soluble
184 T C -0.8721 Soluble
185 E C -2.1576 Soluble
186 Q C 0.0000 Buried
187 D C -1.9074 Soluble
188 S C -2.1491 Soluble
189 K C -2.5445 Soluble
190 D C -2.0112 Soluble
191 S C 0.0000 Buried
192 T C 0.0000 Buried
193 Y C 0.0000 Buried
194 S C 0.0000 Buried
195 L C 0.0000 Buried
196 S C 0.0000 Buried
197 S C 0.0000 Buried
198 T C -0.5558 Soluble
199 L C 0.0000 Buried
200 T C -0.4413 Soluble
201 L C -0.7234 Soluble
202 S C -1.2969 Soluble
203 K C -2.1416 Soluble
204 A C -2.0825 Soluble
205 D C -2.7933 Soluble
206 Y C 0.0000 Buried
207 E C -3.6564 Soluble
208 K C -3.7096 Soluble
209 H C -2.9453 Soluble
210 K C -2.4528 Soluble
211 V C -0.9298 Soluble
212 Y C 0.0000 Buried
213 A C 0.0000 Buried
214 C C 0.0000 Buried
215 E C -0.9990 Soluble
216 V C 0.0000 Buried
217 T C -1.1844 Soluble
218 H C 0.0000 Buried
219 Q C -1.5626 Soluble
220 G C -0.2340 Soluble
221 L C -0.1881 Soluble
222 S C -0.4571 Soluble
223 S C -0.3907 Soluble
224 P C -0.5392 Soluble
225 V C -0.0433 Soluble
226 T C -0.4775 Soluble
227 K C -0.5854 Soluble
228 S C -0.4546 Soluble
229 F C -0.4024 Soluble
230 N C -1.1097 Soluble
231 R C -1.7238 Soluble
232 G C -1.1873 Soluble
233 A C -0.5725 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6542 2.885 CSV PDB
4.5 -0.6902 2.8201 CSV PDB
5.0 -0.7324 2.7439 CSV PDB
5.5 -0.7728 2.663 CSV PDB
6.0 -0.8018 2.5805 CSV PDB
6.5 -0.8116 2.4977 CSV PDB
7.0 -0.8024 2.415 CSV PDB
7.5 -0.7805 2.3331 CSV PDB
8.0 -0.7516 2.3758 CSV PDB
8.5 -0.7173 2.4283 CSV PDB
9.0 -0.6775 2.4806 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH