Zanidatamab / 6bi2

A4DAB304_6bi2_I_M | Source PDB: 6bi2 | Chains: I, M | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.723
Max Score
2.2978
Min Score
-3.2689
Total Score
-307.29
Score CDR
-0.6091
Score non-CDR
-0.7372

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCSRWGGDGFYAMDYWGQGTLVTVSSASTKGPSVFPLAPTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPDIQMTQSPSSLSASVGDRVTITCRASQDVNTAVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKAAYEKHKVYAEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) I (Heavy), M (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.2689
Maximum Score 2.2978
Average Score -0.723
Total Score -307.2913

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E I -2.1821 Soluble
2 V I -1.5533 Soluble
3 Q I -1.4091 Soluble
4 L I 0.0000 Buried
5 V I 0.6529 Aggregation-prone
6 E I 0.0000 Buried
7 S I -0.4441 Soluble
8 G I -0.8527 Soluble
9 G I -0.4012 Soluble
11 G I -0.0389 Soluble
12 L I 0.0977 Aggregation-prone
13 V I -0.5874 Soluble
14 Q I -1.5958 Soluble
15 P I -1.7979 Soluble
16 G I -1.5144 Soluble
17 G I -1.1888 Soluble
18 S I -1.0552 Soluble
19 L I -0.9854 Soluble
20 R I -1.7840 Soluble
21 L I 0.0000 Buried
22 S I -0.4142 Soluble
23 C I 0.0000 Buried
24 A I -0.1741 Soluble
25 A I -0.7201 Soluble
26 S I -1.1469 Soluble
27 G I -1.5763 Soluble
28 F I -1.6268 Soluble
29 N I -2.5050 Soluble
30 I I 0.0000 Buried
35 K I -2.8486 Soluble
36 D I -2.9636 Soluble
37 T I 0.0000 Buried
38 Y I -0.2071 Soluble
39 I I 0.0000 Buried
40 H I 0.0000 Buried
41 W I 0.0000 Buried
42 V I 0.0000 Buried
43 R I 0.0000 Buried
44 Q I -0.8065 Soluble
45 A I -1.1759 Soluble
46 P I -0.9341 Soluble
47 G I -1.4738 Soluble
48 K I -2.4123 Soluble
49 G I -1.6776 Soluble
50 L I 0.0000 Buried
51 E I -1.2435 Soluble
52 W I 0.0000 Buried
53 V I 0.0000 Buried
54 A I 0.0000 Buried
55 R I -0.5247 Soluble
56 I I 0.0000 Buried
57 Y I -0.3713 Soluble
58 P I -1.0876 Soluble
59 T I -1.2460 Soluble
62 N I -1.0296 Soluble
63 G I -0.3569 Soluble
64 Y I 0.5471 Aggregation-prone
65 T I -0.3576 Soluble
66 R I -1.8053 Soluble
67 Y I -1.8110 Soluble
68 A I -1.8559 Soluble
69 D I -2.6947 Soluble
70 S I -1.7836 Soluble
71 V I 0.0000 Buried
72 K I -2.8471 Soluble
74 G I -1.7242 Soluble
75 R I -1.4090 Soluble
76 F I 0.0000 Buried
77 T I -1.0260 Soluble
78 I I 0.0000 Buried
79 S I -0.1466 Soluble
80 A I -0.9394 Soluble
81 D I -1.5066 Soluble
82 T I -1.6999 Soluble
83 S I -1.6097 Soluble
84 K I -2.2545 Soluble
85 N I -1.8463 Soluble
86 T I -0.9693 Soluble
87 A I 0.0000 Buried
88 Y I 0.0000 Buried
89 L I 0.0000 Buried
90 Q I -1.1047 Soluble
91 M I 0.0000 Buried
92 N I -1.3236 Soluble
93 S I -1.2819 Soluble
94 L I 0.0000 Buried
95 R I -2.5212 Soluble
96 A I -1.8177 Soluble
97 E I -2.3333 Soluble
98 D I 0.0000 Buried
99 T I -0.6905 Soluble
100 A I 0.0000 Buried
101 V I 0.3831 Aggregation-prone
102 Y I 0.0000 Buried
103 Y I 0.0000 Buried
104 C I 0.0000 Buried
105 S I 0.0000 Buried
106 R I 0.0014 Aggregation-prone
107 W I 0.0000 Buried
108 G I -0.5318 Soluble
109 G I -1.2361 Soluble
110 D I -2.1352 Soluble
111 G I -1.0618 Soluble
112 F I -0.1909 Soluble
113 Y I 0.4549 Aggregation-prone
114 A I 0.0000 Buried
115 M I 0.0000 Buried
116 D I -0.1879 Soluble
117 Y I -0.3455 Soluble
118 W I -0.5273 Soluble
119 G I 0.0000 Buried
120 Q I -1.3782 Soluble
121 G I -0.5193 Soluble
122 T I 0.0312 Aggregation-prone
123 L I 0.2835 Aggregation-prone
124 V I 0.0000 Buried
125 T I -0.3923 Soluble
126 V I 0.0000 Buried
127 S I -0.9508 Soluble
128 S I -0.6793 Soluble
129 A I -0.4395 Soluble
130 S I -0.5512 Soluble
131 T I -0.7225 Soluble
132 K I -1.1518 Soluble
133 G I -1.3838 Soluble
134 P I 0.0000 Buried
135 S I -0.4488 Soluble
136 V I 0.0000 Buried
137 F I 0.0000 Buried
138 P I -1.0629 Soluble
139 L I 0.0000 Buried
140 A I -0.6120 Soluble
141 P I -0.3710 Soluble
142 T I -0.2948 Soluble
143 A I -0.1629 Soluble
144 A I 0.0000 Buried
145 L I 0.0000 Buried
146 G I 0.0000 Buried
147 C I 0.0000 Buried
148 L I 0.0000 Buried
149 V I 0.0000 Buried
150 K I 0.0000 Buried
151 D I -0.3054 Soluble
152 Y I 0.0000 Buried
153 F I 0.0000 Buried
154 P I 0.0000 Buried
155 E I -0.5542 Soluble
156 P I -0.8485 Soluble
157 V I -0.8723 Soluble
158 T I -0.7624 Soluble
159 V I -0.3167 Soluble
160 S I -0.4852 Soluble
161 W I 0.0000 Buried
162 N I -0.7391 Soluble
163 S I -0.6758 Soluble
164 G I -0.5719 Soluble
165 A I -0.2418 Soluble
166 L I -0.0143 Soluble
167 T I -0.1865 Soluble
168 S I -0.2036 Soluble
169 G I -0.2425 Soluble
170 V I 0.1607 Aggregation-prone
171 H I -0.3127 Soluble
172 T I -0.1831 Soluble
173 F I 0.0000 Buried
174 P I -0.3352 Soluble
175 A I 0.2049 Aggregation-prone
176 V I 0.5199 Aggregation-prone
177 L I 1.1984 Aggregation-prone
178 Q I 0.3098 Aggregation-prone
179 S I -0.1472 Soluble
180 S I -0.1822 Soluble
181 G I 0.0962 Aggregation-prone
182 L I 0.2234 Aggregation-prone
183 Y I 0.5121 Aggregation-prone
184 S I 0.0000 Buried
185 L I 0.0000 Buried
186 S I 0.0000 Buried
187 S I 0.0000 Buried
188 V I 0.0000 Buried
189 V I 0.0000 Buried
190 T I -0.1650 Soluble
191 V I 0.0000 Buried
192 P I -0.4889 Soluble
193 S I -0.4400 Soluble
194 S I -0.5154 Soluble
195 S I -0.5615 Soluble
196 L I -0.6845 Soluble
197 G I -0.9873 Soluble
198 T I -0.8290 Soluble
199 Q I -1.1534 Soluble
200 T I -0.9697 Soluble
201 Y I 0.0000 Buried
202 I I -1.1348 Soluble
203 C I 0.0000 Buried
204 N I -1.6198 Soluble
205 V I 0.0000 Buried
206 N I -2.4872 Soluble
207 H I 0.0000 Buried
208 K I -2.9221 Soluble
209 P I -1.6558 Soluble
210 S I -1.8900 Soluble
211 N I -2.7083 Soluble
212 T I -2.2412 Soluble
213 K I -2.9077 Soluble
214 V I -1.7595 Soluble
215 D I -2.6014 Soluble
216 K I -1.9046 Soluble
217 K I -2.1087 Soluble
218 V I 0.0000 Buried
219 E I -1.9095 Soluble
220 P I -0.8682 Soluble
1 D M -2.2544 Soluble
2 I M 0.0000 Buried
3 Q M -2.3033 Soluble
4 M M 0.0000 Buried
5 T M -1.3891 Soluble
6 Q M 0.0000 Buried
7 S M -0.9874 Soluble
8 P M -0.7666 Soluble
9 S M -1.0527 Soluble
10 S M -1.0317 Soluble
11 L M -0.6613 Soluble
12 S M -0.8018 Soluble
13 A M 0.0000 Buried
14 S M -0.9758 Soluble
15 V M -0.2338 Soluble
16 G M -0.7998 Soluble
17 D M -1.9761 Soluble
18 R M -2.3748 Soluble
19 V M 0.0000 Buried
20 T M -0.6644 Soluble
21 I M 0.0000 Buried
22 T M -0.7676 Soluble
23 C M 0.0000 Buried
24 R M -2.8541 Soluble
25 A M 0.0000 Buried
26 S M -2.1897 Soluble
27 Q M -2.7493 Soluble
28 D M -2.8431 Soluble
29 V M 0.0000 Buried
36 N M -1.8845 Soluble
37 T M -0.9436 Soluble
38 A M -0.0406 Soluble
39 V M 0.0000 Buried
40 A M 0.0000 Buried
41 W M 0.0000 Buried
42 Y M 0.0000 Buried
43 Q M 0.0000 Buried
44 Q M 0.0000 Buried
45 K M -1.6632 Soluble
46 P M -1.3339 Soluble
47 G M -1.6177 Soluble
48 K M -2.5923 Soluble
49 A M -1.6828 Soluble
50 P M 0.0000 Buried
51 K M -1.8370 Soluble
52 L M 0.0000 Buried
53 L M 0.0000 Buried
54 I M 0.0000 Buried
55 Y M 1.2698 Aggregation-prone
56 S M 0.5346 Aggregation-prone
57 A M 0.0000 Buried
65 S M 0.7583 Aggregation-prone
66 F M 2.2978 Aggregation-prone
67 L M 1.6187 Aggregation-prone
68 Y M 0.6753 Aggregation-prone
69 S M 0.0157 Aggregation-prone
70 G M -0.4576 Soluble
71 V M 0.0000 Buried
72 P M -0.3238 Soluble
74 S M -0.3567 Soluble
75 R M -0.6752 Soluble
76 F M 0.0000 Buried
77 S M 0.1399 Aggregation-prone
78 G M 0.1210 Aggregation-prone
79 S M -0.5515 Soluble
80 R M -1.5830 Soluble
83 S M -1.3296 Soluble
84 G M -1.8413 Soluble
85 T M -2.2845 Soluble
86 D M -1.9456 Soluble
87 F M 0.0000 Buried
88 T M -0.7457 Soluble
89 L M 0.0000 Buried
90 T M -0.6223 Soluble
91 I M 0.0000 Buried
92 S M -1.3482 Soluble
93 S M -1.1059 Soluble
94 L M 0.0000 Buried
95 Q M -0.7565 Soluble
96 P M -1.4428 Soluble
97 E M -2.1181 Soluble
98 D M 0.0000 Buried
99 F M -1.1594 Soluble
100 A M 0.0000 Buried
101 T M 0.0000 Buried
102 Y M 0.0000 Buried
103 Y M 0.0000 Buried
104 C M 0.0000 Buried
105 Q M 0.0000 Buried
106 Q M 0.0000 Buried
107 H M 0.5237 Aggregation-prone
108 Y M 0.8190 Aggregation-prone
109 T M 0.2463 Aggregation-prone
114 T M -0.4372 Soluble
115 P M -1.0161 Soluble
116 P M 0.0000 Buried
117 T M -0.7337 Soluble
118 F M -0.5558 Soluble
119 G M 0.0000 Buried
120 Q M -1.9374 Soluble
121 G M 0.0000 Buried
122 T M 0.0000 Buried
123 K M -2.0022 Soluble
124 V M 0.0000 Buried
125 E M -1.2772 Soluble
126 I M 0.0000 Buried
127 K M -1.5129 Soluble
128 R M -1.0404 Soluble
129 T M -0.1851 Soluble
130 V M 0.2628 Aggregation-prone
131 A M -0.0774 Soluble
132 A M -0.0955 Soluble
133 P M 0.0000 Buried
134 S M -0.1395 Soluble
135 V M 0.0000 Buried
136 F M 0.1489 Aggregation-prone
137 I M 0.1496 Aggregation-prone
138 F M 0.0000 Buried
139 P M -0.3917 Soluble
140 P M -0.8804 Soluble
141 S M -1.6958 Soluble
142 D M -3.0223 Soluble
143 E M -3.1707 Soluble
144 Q M 0.0000 Buried
145 L M -2.3734 Soluble
146 K M -2.8984 Soluble
147 S M -1.8152 Soluble
148 G M -1.3223 Soluble
149 T M -0.9544 Soluble
150 A M 0.0000 Buried
151 S M 0.0000 Buried
152 V M 0.0000 Buried
153 V M 0.0000 Buried
154 C M 0.0000 Buried
155 L M 0.0000 Buried
156 L M 0.0000 Buried
157 N M 0.0000 Buried
158 N M -0.8583 Soluble
159 F M 0.0000 Buried
160 Y M 0.0000 Buried
161 P M -1.8515 Soluble
162 R M -3.1264 Soluble
163 E M -3.2689 Soluble
164 A M -2.3451 Soluble
165 K M -2.3955 Soluble
166 V M -1.0087 Soluble
167 Q M -0.5653 Soluble
168 W M 0.0000 Buried
169 K M -0.6210 Soluble
170 V M 0.0000 Buried
171 D M -1.7639 Soluble
172 N M -1.5454 Soluble
173 A M -0.3023 Soluble
174 L M 0.6972 Aggregation-prone
175 Q M -0.2828 Soluble
176 S M -0.6034 Soluble
177 G M -1.1963 Soluble
178 N M -1.4431 Soluble
179 S M -1.4659 Soluble
180 Q M -1.6008 Soluble
181 E M -1.9915 Soluble
182 S M -1.0549 Soluble
183 V M -0.8944 Soluble
184 T M -1.1704 Soluble
185 E M -2.1792 Soluble
186 Q M 0.0000 Buried
187 D M -2.0466 Soluble
188 S M -2.0754 Soluble
189 K M -2.4426 Soluble
190 D M -1.7302 Soluble
191 S M -1.9102 Soluble
192 T M 0.0000 Buried
193 Y M 0.0000 Buried
194 S M 0.0000 Buried
195 L M 0.0000 Buried
196 S M 0.0000 Buried
197 S M 0.0000 Buried
198 T M -0.7942 Soluble
199 L M 0.0000 Buried
200 T M -0.5355 Soluble
201 L M -0.5077 Soluble
202 S M -0.7576 Soluble
203 K M -1.8266 Soluble
204 A M -1.3587 Soluble
205 A M -1.3446 Soluble
206 Y M 0.0000 Buried
207 E M -2.9185 Soluble
208 K M -3.0134 Soluble
209 H M -2.5695 Soluble
210 K M -2.9703 Soluble
211 V M -1.3866 Soluble
212 Y M 0.0000 Buried
213 A M -0.7932 Soluble
215 E M -0.7952 Soluble
216 V M 0.0000 Buried
217 T M -1.1972 Soluble
218 H M 0.0000 Buried
219 Q M -1.7675 Soluble
220 G M -0.4723 Soluble
221 L M -0.2819 Soluble
222 S M -0.4935 Soluble
223 S M -0.4297 Soluble
224 P M -0.5106 Soluble
225 V M 0.0109 Aggregation-prone
226 T M -0.4503 Soluble
227 K M -0.6794 Soluble
228 S M -0.5552 Soluble
229 F M -0.8041 Soluble
230 N M -1.7723 Soluble
231 R M -2.3033 Soluble
232 G M -1.8578 Soluble
233 E M -2.1394 Soluble
234 C M -0.4727 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.726 3.9017 CSV PDB
4.5 -0.7711 3.9017 CSV PDB
5.0 -0.8234 3.9017 CSV PDB
5.5 -0.8732 3.9017 CSV PDB
6.0 -0.9087 3.9017 CSV PDB
6.5 -0.9207 3.9017 CSV PDB
7.0 -0.9092 3.9017 CSV PDB
7.5 -0.8821 3.9017 CSV PDB
8.0 -0.8463 3.9017 CSV PDB
8.5 -0.8038 3.9015 CSV PDB
9.0 -0.7543 3.9011 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH