Verenafusp / 5kqv

A4DAB293_5kqv_C_D | Source PDB: 5kqv | Chains: C, D | Done
99.55
Yes
10 Å
Yes
Avg A4D Score
-0.6937
Max Score
1.5426
Min Score
-3.8481
Total Score
-294.81
Score CDR
-0.6135
Score non-CDR
-0.702

Calculation Parameters

Chain Sequence(s) VQLQQSGPELVKPGALVKISCKASGYTFTNYDIHWVKQRPGQGLEWIGWIYPGDGSTKYNEKFKGKATLTADKSSSTAYMHLSSLTSEKSAVYFCAREWAYWGQGTLVTVSAAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTITCNVAHPASSTKVDKKIEPRGPTIDIQMTQSPSSLSASLGERVSLTCRASQDIGGNLYWLQQGPDGTIKRLIYATSSLDSGVPKRFSGSRSGSDYSLTISSLESEDFVDYYCLQYSSSPWTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRGEC input.pdb
Selected Chain(s) C (Heavy), D (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.8481
Maximum Score 1.5426
Average Score -0.6937
Total Score -294.8062

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V C 1.0875 Aggregation-prone
3 Q C -0.8058 Soluble
4 L C 0.0000 Buried
5 Q C -1.4965 Soluble
6 Q C 0.0000 Buried
7 S C -0.7582 Soluble
8 G C -0.3061 Soluble
9 P C -0.1381 Soluble
11 E C -0.0183 Soluble
12 L C 0.6623 Aggregation-prone
13 V C 0.0726 Aggregation-prone
14 K C -1.0857 Soluble
15 P C -0.9762 Soluble
16 G C -0.6693 Soluble
17 A C 0.0158 Aggregation-prone
18 L C 0.7991 Aggregation-prone
19 V C 0.0000 Buried
20 K C -1.0170 Soluble
21 I C 0.0000 Buried
22 S C -0.7265 Soluble
23 C C 0.0000 Buried
24 K C -1.7384 Soluble
25 A C 0.0000 Buried
26 S C -0.5346 Soluble
27 G C 0.0783 Aggregation-prone
28 Y C 0.0779 Aggregation-prone
29 T C -0.2196 Soluble
30 F C 0.0000 Buried
35 T C -1.1035 Soluble
36 N C -1.1412 Soluble
37 Y C -0.5479 Soluble
38 D C -0.5498 Soluble
39 I C 0.0000 Buried
40 H C 0.0000 Buried
41 W C 0.0000 Buried
42 V C 0.0000 Buried
43 K C 0.0000 Buried
44 Q C -1.0867 Soluble
45 R C -1.7576 Soluble
46 P C -1.4707 Soluble
47 G C -1.4094 Soluble
48 Q C -2.0612 Soluble
49 G C -1.4321 Soluble
50 L C 0.0000 Buried
51 E C -1.4690 Soluble
52 W C 0.0000 Buried
53 I C 0.0000 Buried
54 G C 0.0000 Buried
55 W C -0.1692 Soluble
56 I C 0.0000 Buried
57 Y C -0.4975 Soluble
58 P C 0.0000 Buried
59 G C -1.5618 Soluble
62 D C -1.9666 Soluble
63 G C -1.4773 Soluble
64 S C -0.9337 Soluble
65 T C -0.5624 Soluble
66 K C -1.0002 Soluble
67 Y C -1.5618 Soluble
68 N C -2.2968 Soluble
69 E C -3.4821 Soluble
70 K C -3.3530 Soluble
71 F C 0.0000 Buried
72 K C -3.2091 Soluble
74 G C -2.1135 Soluble
75 K C -1.6929 Soluble
76 A C 0.0000 Buried
77 T C -0.6975 Soluble
78 L C 0.0000 Buried
79 T C -0.4165 Soluble
80 A C -1.0245 Soluble
81 D C -1.5445 Soluble
82 K C -1.7859 Soluble
83 S C -1.0599 Soluble
84 S C -1.0187 Soluble
85 S C -1.1744 Soluble
86 T C 0.0000 Buried
87 A C 0.0000 Buried
88 Y C -0.4668 Soluble
89 M C 0.0000 Buried
90 H C -0.4053 Soluble
91 L C 0.0000 Buried
92 S C -0.1235 Soluble
93 S C -0.3866 Soluble
94 L C 0.0000 Buried
95 T C -0.9129 Soluble
96 S C -0.9375 Soluble
97 E C 0.0000 Buried
98 K C -1.9580 Soluble
99 S C -0.8466 Soluble
100 A C 0.0000 Buried
101 V C -0.0059 Soluble
102 Y C 0.0000 Buried
103 F C 0.0000 Buried
104 C C 0.0000 Buried
105 A C 0.0000 Buried
106 R C -0.3461 Soluble
107 E C -0.7033 Soluble
115 W C 0.0000 Buried
116 A C -0.1043 Soluble
117 Y C 0.4029 Aggregation-prone
118 W C 0.0000 Buried
119 G C 0.0000 Buried
120 Q C -1.4941 Soluble
121 G C -0.6738 Soluble
122 T C 0.0000 Buried
123 L C 0.5065 Aggregation-prone
124 V C 0.0000 Buried
125 T C 0.0000 Buried
126 V C 0.0000 Buried
127 S C 0.0000 Buried
128 A C -0.8302 Soluble
129 A C -1.1102 Soluble
130 K C -1.7771 Soluble
131 T C -0.9441 Soluble
132 T C -0.4953 Soluble
133 A C -0.4354 Soluble
134 P C 0.0000 Buried
135 S C -0.1750 Soluble
136 V C 0.0000 Buried
137 Y C 0.0000 Buried
138 P C 0.0000 Buried
139 L C 0.0000 Buried
140 A C -0.2019 Soluble
141 P C -0.1797 Soluble
142 V C 0.2061 Aggregation-prone
143 C C -1.0899 Soluble
144 G C -1.8553 Soluble
145 D C -1.7639 Soluble
146 T C -0.8645 Soluble
147 T C -0.6613 Soluble
148 G C -0.6238 Soluble
149 S C -0.3476 Soluble
150 S C -0.0947 Soluble
151 V C 0.3635 Aggregation-prone
152 T C 0.4182 Aggregation-prone
153 L C 0.2070 Aggregation-prone
154 G C 0.0000 Buried
155 C C 0.0000 Buried
156 L C 0.0000 Buried
157 V C 0.0000 Buried
158 K C -0.2646 Soluble
159 G C -0.3639 Soluble
160 Y C 0.0000 Buried
161 F C 0.0000 Buried
162 P C -0.5972 Soluble
163 E C -0.8697 Soluble
164 P C -0.6668 Soluble
165 V C -0.3458 Soluble
166 T C -0.2762 Soluble
167 L C -0.1298 Soluble
168 T C -0.3995 Soluble
169 W C 0.0000 Buried
170 N C -0.9555 Soluble
171 S C -0.8081 Soluble
172 G C -0.8151 Soluble
173 S C -0.6202 Soluble
174 L C -0.1582 Soluble
175 S C -0.4046 Soluble
176 S C -0.4358 Soluble
177 G C -0.1566 Soluble
178 V C 0.1580 Aggregation-prone
179 H C -0.2859 Soluble
180 T C 0.0741 Aggregation-prone
181 F C 0.0000 Buried
182 P C -0.2446 Soluble
183 A C 0.0408 Aggregation-prone
184 V C 0.2515 Aggregation-prone
185 L C -0.2457 Soluble
186 Q C -0.8548 Soluble
187 S C -0.7814 Soluble
188 D C -1.2114 Soluble
189 L C -0.5501 Soluble
190 Y C -0.1831 Soluble
191 T C -0.0491 Soluble
192 L C 0.0000 Buried
193 S C 0.0000 Buried
194 S C 0.0000 Buried
195 S C 0.0000 Buried
196 V C 0.0000 Buried
197 T C 0.2292 Aggregation-prone
198 V C 0.3039 Aggregation-prone
199 T C -0.1293 Soluble
200 S C -0.3101 Soluble
201 S C -0.3266 Soluble
202 T C -0.0424 Soluble
203 I C -0.2479 Soluble
204 T C -1.2724 Soluble
205 C C 0.0000 Buried
206 N C -1.2211 Soluble
207 V C 0.0000 Buried
208 A C -0.7976 Soluble
209 H C 0.0000 Buried
210 P C -0.6976 Soluble
211 A C -0.3615 Soluble
212 S C -0.4684 Soluble
213 S C -0.6734 Soluble
214 T C -0.8530 Soluble
215 K C -1.8744 Soluble
216 V C -1.0146 Soluble
217 D C -2.0383 Soluble
218 K C -2.0335 Soluble
219 K C -2.6220 Soluble
220 I C 0.0000 Buried
221 E C -2.2473 Soluble
222 P C -1.2074 Soluble
223 R C -1.0887 Soluble
224 G C -0.9002 Soluble
225 P C 0.0242 Aggregation-prone
226 T C 0.6368 Aggregation-prone
227 I C 1.5426 Aggregation-prone
1 D D -2.3306 Soluble
2 I D 0.0000 Buried
3 Q D -2.2820 Soluble
4 M D 0.0000 Buried
5 T D -1.3360 Soluble
6 Q D -1.0770 Soluble
7 S D -0.7857 Soluble
8 P D -0.5194 Soluble
9 S D -0.6864 Soluble
10 S D -0.5383 Soluble
11 L D -0.2708 Soluble
12 S D -0.6206 Soluble
13 A D 0.0000 Buried
14 S D -0.7750 Soluble
15 L D -0.1765 Soluble
16 G D -1.1144 Soluble
17 E D -2.0462 Soluble
18 R D -2.4830 Soluble
19 V D 0.0000 Buried
20 S D -0.6381 Soluble
21 L D 0.0000 Buried
22 T D -0.9287 Soluble
23 C D 0.0000 Buried
24 R D -2.8935 Soluble
25 A D 0.0000 Buried
26 S D -2.2888 Soluble
27 Q D -2.9258 Soluble
28 D D -2.8803 Soluble
29 I D 0.0000 Buried
36 G D -1.3235 Soluble
37 G D -0.9485 Soluble
38 N D -0.7862 Soluble
39 L D 0.0000 Buried
40 Y D 0.0000 Buried
41 W D 0.0000 Buried
42 L D 0.0000 Buried
43 Q D 0.0000 Buried
44 Q D 0.0000 Buried
45 G D -1.3151 Soluble
46 P D -1.3924 Soluble
47 D D -2.1139 Soluble
48 G D -1.4509 Soluble
49 T D -1.3344 Soluble
50 I D 0.0000 Buried
51 K D -1.5186 Soluble
52 R D -0.6432 Soluble
53 L D 0.0000 Buried
54 I D 0.0000 Buried
55 Y D 0.4027 Aggregation-prone
56 A D -0.0016 Soluble
57 T D 0.0000 Buried
65 S D -0.4258 Soluble
66 S D 0.0396 Aggregation-prone
67 L D -0.2204 Soluble
68 D D -0.6020 Soluble
69 S D -0.4933 Soluble
70 G D -0.7637 Soluble
71 V D -0.8487 Soluble
72 P D -1.1060 Soluble
74 K D -1.9483 Soluble
75 R D -1.5527 Soluble
76 F D 0.0000 Buried
77 S D -0.6822 Soluble
78 G D 0.0000 Buried
79 S D -0.9644 Soluble
80 R D -1.7573 Soluble
83 S D -1.3185 Soluble
84 G D -1.4512 Soluble
85 S D -1.9881 Soluble
86 D D -1.7131 Soluble
87 Y D 0.0000 Buried
88 S D -0.9129 Soluble
89 L D 0.0000 Buried
90 T D -0.6771 Soluble
91 I D 0.0000 Buried
92 S D -1.7374 Soluble
93 S D -1.5977 Soluble
94 L D 0.0000 Buried
95 E D -1.4668 Soluble
96 S D -1.1535 Soluble
97 E D -1.9822 Soluble
98 D D 0.0000 Buried
99 F D -0.4464 Soluble
100 V D -0.4888 Soluble
101 D D -0.5829 Soluble
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 L D 0.0000 Buried
106 Q D 0.0000 Buried
107 Y D 0.0401 Aggregation-prone
108 S D -0.6728 Soluble
109 S D -0.8135 Soluble
114 S D -0.5845 Soluble
115 P D -0.9963 Soluble
116 W D -0.3889 Soluble
117 T D -0.6784 Soluble
118 F D -0.3155 Soluble
119 G D 0.0000 Buried
120 G D -1.0457 Soluble
121 G D 0.0000 Buried
122 T D 0.0000 Buried
123 K D -0.7415 Soluble
124 L D 0.0000 Buried
125 E D 0.0000 Buried
126 I D -0.3782 Soluble
127 K D -1.7618 Soluble
128 R D -1.7260 Soluble
129 A D -1.4460 Soluble
130 D D -2.4480 Soluble
131 A D -1.2323 Soluble
132 A D -0.6706 Soluble
133 P D 0.0000 Buried
134 T D -0.0318 Soluble
135 V D 0.1792 Aggregation-prone
136 S D 0.2716 Aggregation-prone
137 I D 0.0000 Buried
138 F D 0.0000 Buried
139 P D 0.0000 Buried
140 P D 0.0000 Buried
141 S D 0.0000 Buried
142 S D -0.7234 Soluble
143 E D -1.2077 Soluble
144 Q D 0.0000 Buried
145 L D -1.0183 Soluble
146 T D -0.6864 Soluble
147 S D -0.7177 Soluble
148 G D -1.0617 Soluble
149 G D -0.9722 Soluble
150 A D 0.0000 Buried
151 S D 0.0000 Buried
152 V D 0.0000 Buried
153 V D 0.0000 Buried
154 C D 0.0000 Buried
155 F D 0.0000 Buried
156 L D 0.0000 Buried
157 N D 0.0000 Buried
158 N D -0.7315 Soluble
159 F D 0.0000 Buried
160 Y D 0.0000 Buried
161 P D -2.1851 Soluble
162 K D -2.3941 Soluble
163 D D -2.7350 Soluble
164 I D -1.7643 Soluble
165 N D -1.7979 Soluble
166 V D -0.6427 Soluble
167 K D -1.4602 Soluble
168 W D 0.0000 Buried
169 K D -2.3066 Soluble
170 I D 0.0000 Buried
171 D D -3.1460 Soluble
172 G D -2.1277 Soluble
173 S D -2.2128 Soluble
174 E D -3.1521 Soluble
175 R D -2.6388 Soluble
176 Q D -2.4878 Soluble
177 N D -2.0809 Soluble
178 G D -1.0116 Soluble
179 V D -0.5754 Soluble
180 L D -0.1351 Soluble
181 N D 0.0886 Aggregation-prone
182 S D 0.1693 Aggregation-prone
183 W D 0.2631 Aggregation-prone
184 T D -0.7388 Soluble
185 D D -2.0230 Soluble
186 Q D -1.6906 Soluble
187 D D -1.8981 Soluble
188 S D -1.6724 Soluble
189 K D -2.2354 Soluble
190 D D -1.5949 Soluble
191 S D -1.7517 Soluble
192 T D 0.0000 Buried
193 Y D 0.0000 Buried
194 S D 0.0000 Buried
195 M D 0.0000 Buried
196 S D 0.0000 Buried
197 S D 0.0000 Buried
198 T D 0.0482 Aggregation-prone
199 L D 0.0000 Buried
200 T D -0.2887 Soluble
201 L D 0.0000 Buried
202 T D -1.3823 Soluble
203 K D -2.3969 Soluble
204 D D -3.2973 Soluble
205 E D -2.5735 Soluble
206 Y D 0.0000 Buried
207 E D -3.7128 Soluble
208 R D -3.8481 Soluble
209 H D -3.2989 Soluble
210 N D -3.2958 Soluble
211 S D -2.2597 Soluble
212 Y D 0.0000 Buried
213 T D -1.1003 Soluble
214 C D 0.0000 Buried
215 E D -0.3288 Soluble
216 A D 0.0000 Buried
217 T D -0.9736 Soluble
218 H D 0.0000 Buried
219 K D -2.5776 Soluble
220 T D -1.3455 Soluble
221 S D -0.7035 Soluble
222 T D -0.5473 Soluble
223 S D -0.3214 Soluble
224 P D -0.2753 Soluble
225 I D 0.6483 Aggregation-prone
226 V D 0.8554 Aggregation-prone
227 K D 0.0576 Aggregation-prone
228 S D -0.3749 Soluble
229 F D 0.0000 Buried
230 N D -2.5568 Soluble
231 R D -2.7341 Soluble
232 G D -2.2444 Soluble
233 E D -2.4116 Soluble
234 C D -1.3279 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6469 3.4711 CSV PDB
4.5 -0.6963 3.4417 CSV PDB
5.0 -0.7523 3.3966 CSV PDB
5.5 -0.8063 3.3431 CSV PDB
6.0 -0.847 3.2896 CSV PDB
6.5 -0.8659 3.2448 CSV PDB
7.0 -0.8631 3.2156 CSV PDB
7.5 -0.8451 3.2017 CSV PDB
8.0 -0.8171 3.1964 CSV PDB
8.5 -0.7803 3.1947 CSV PDB
9.0 -0.7348 3.1941 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH