Anvatabart / 5xhf

A4DAB021_5xhf_D_C | Source PDB: 5xhf | Chains: D, C | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7168
Max Score
2.161
Min Score
-3.5471
Total Score
-310.37
Score CDR
-0.6094
Score non-CDR
-0.7299

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCSRWGGDGFYAMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPDIQMTQSPSSLSASVGDRVTITCRASQDVNTAVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) D (Heavy), C (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.5471
Maximum Score 2.161
Average Score -0.7168
Total Score -310.3746

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E D -2.1412 Soluble
2 V D -1.2932 Soluble
3 Q D -1.1838 Soluble
4 L D 0.0000 Buried
5 V D 1.0681 Aggregation-prone
6 E D 0.2250 Aggregation-prone
7 S D -0.3462 Soluble
8 G D -0.9112 Soluble
9 G D -0.5138 Soluble
11 G D -0.0976 Soluble
12 L D 0.0316 Aggregation-prone
13 V D -0.6451 Soluble
14 Q D -1.6689 Soluble
15 P D -1.8924 Soluble
16 G D -1.6501 Soluble
17 G D -1.1195 Soluble
18 S D -1.0532 Soluble
19 L D -0.9931 Soluble
20 R D -2.0694 Soluble
21 L D 0.0000 Buried
22 S D -0.4142 Soluble
23 C D 0.0000 Buried
24 A D -0.0563 Soluble
25 A D 0.0000 Buried
26 S D -0.9228 Soluble
27 G D -1.4268 Soluble
28 F D -1.4061 Soluble
29 N D -2.3369 Soluble
30 I D 0.0000 Buried
35 K D -2.4088 Soluble
36 D D -2.8012 Soluble
37 T D 0.0000 Buried
38 Y D -0.1421 Soluble
39 I D 0.0000 Buried
40 H D 0.0000 Buried
41 W D 0.0000 Buried
42 V D 0.0000 Buried
43 R D -0.8080 Soluble
44 Q D -1.0884 Soluble
45 A D -1.3571 Soluble
46 P D -0.9504 Soluble
47 G D -1.4738 Soluble
48 K D -2.5197 Soluble
49 G D -1.8512 Soluble
50 L D 0.0000 Buried
51 E D -1.7530 Soluble
52 W D 0.0000 Buried
53 V D 0.0000 Buried
54 A D 0.0000 Buried
55 R D -0.5655 Soluble
56 I D 0.0000 Buried
57 Y D -0.5002 Soluble
58 P D -1.0490 Soluble
59 T D -1.1973 Soluble
62 N D -1.0920 Soluble
63 G D -0.4239 Soluble
64 Y D 0.4443 Aggregation-prone
65 T D -0.4803 Soluble
66 R D -1.9936 Soluble
67 Y D -1.8442 Soluble
68 A D -1.9466 Soluble
69 D D -2.6536 Soluble
70 S D -1.7243 Soluble
71 V D 0.0000 Buried
72 K D -2.8466 Soluble
74 G D -1.7194 Soluble
75 R D -1.2287 Soluble
76 F D 0.0000 Buried
77 T D -1.0808 Soluble
78 I D 0.0000 Buried
79 S D -0.1516 Soluble
80 A D -0.6906 Soluble
81 D D -1.2586 Soluble
82 T D -1.6214 Soluble
83 S D -1.5035 Soluble
84 K D -2.2126 Soluble
85 N D -1.7726 Soluble
86 T D -0.8392 Soluble
87 A D 0.0000 Buried
88 Y D -0.5728 Soluble
89 L D 0.0000 Buried
90 Q D -1.2185 Soluble
91 M D 0.0000 Buried
92 N D -1.3804 Soluble
93 S D -1.3664 Soluble
94 L D 0.0000 Buried
95 R D -2.8641 Soluble
96 A D -2.0354 Soluble
97 E D -2.4455 Soluble
98 D D 0.0000 Buried
99 T D -0.7728 Soluble
100 A D 0.0000 Buried
101 V D 0.1408 Aggregation-prone
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 S D 0.0000 Buried
106 R D -0.0500 Soluble
107 W D 0.0000 Buried
108 G D -0.7350 Soluble
109 G D -1.4652 Soluble
110 D D -2.1566 Soluble
111 G D -1.0199 Soluble
112 F D -0.1074 Soluble
113 Y D 0.5265 Aggregation-prone
114 A D 0.0000 Buried
115 M D 0.0000 Buried
116 D D -0.1592 Soluble
117 Y D -0.3868 Soluble
118 W D -0.4783 Soluble
119 G D 0.0000 Buried
120 Q D -1.3115 Soluble
121 G D -0.6550 Soluble
122 T D -0.0901 Soluble
123 L D 0.2249 Aggregation-prone
124 V D 0.0000 Buried
125 T D 0.0000 Buried
126 V D 0.0000 Buried
127 S D -1.0649 Soluble
128 S D -0.7013 Soluble
129 A D -0.4478 Soluble
130 S D -0.5333 Soluble
131 T D -0.7074 Soluble
132 K D -1.1521 Soluble
133 G D -1.3759 Soluble
134 P D 0.0000 Buried
135 S D -0.2816 Soluble
136 V D 0.0000 Buried
137 F D 0.0000 Buried
138 P D -0.9762 Soluble
139 L D 0.0000 Buried
140 A D -0.6574 Soluble
141 P D 0.0000 Buried
142 S D -0.7148 Soluble
143 S D -1.1954 Soluble
144 K D -2.0850 Soluble
145 S D -1.3345 Soluble
146 T D -0.8911 Soluble
147 S D -0.9441 Soluble
148 G D -0.8178 Soluble
149 G D -0.6781 Soluble
150 T D -0.4765 Soluble
151 A D 0.0000 Buried
152 A D 0.0000 Buried
153 L D 0.0000 Buried
154 G D 0.0000 Buried
155 C D 0.0000 Buried
156 L D 0.0000 Buried
157 V D 0.0000 Buried
158 K D 0.0000 Buried
159 D D -0.3607 Soluble
160 Y D 0.0000 Buried
161 F D 0.0000 Buried
162 P D 0.0000 Buried
163 E D -0.6168 Soluble
164 P D -0.7806 Soluble
165 V D -0.8370 Soluble
166 T D -0.7289 Soluble
167 V D -0.2601 Soluble
168 S D -0.4182 Soluble
169 W D 0.0000 Buried
170 N D -0.7066 Soluble
171 S D -0.5845 Soluble
172 G D -0.5818 Soluble
173 A D -0.2411 Soluble
174 L D 0.0068 Aggregation-prone
175 T D -0.1693 Soluble
176 S D -0.1965 Soluble
177 G D -0.4468 Soluble
178 V D 0.1643 Aggregation-prone
179 H D -0.3180 Soluble
180 T D -0.1970 Soluble
181 F D 0.0000 Buried
182 P D -0.3145 Soluble
183 A D 0.2126 Aggregation-prone
184 V D 0.4965 Aggregation-prone
185 L D 0.9528 Aggregation-prone
186 Q D 0.1962 Aggregation-prone
187 S D -0.1081 Soluble
188 S D -0.2184 Soluble
189 G D 0.0220 Aggregation-prone
190 L D 0.1162 Aggregation-prone
191 Y D 0.4623 Aggregation-prone
192 S D 0.0000 Buried
193 L D 0.0000 Buried
194 S D 0.0000 Buried
195 S D 0.0000 Buried
196 V D 0.0000 Buried
197 V D 0.0000 Buried
198 T D -0.1233 Soluble
199 V D 0.0000 Buried
200 P D -0.4992 Soluble
201 S D -0.5715 Soluble
202 S D -0.6336 Soluble
203 S D -0.5083 Soluble
204 L D -0.7515 Soluble
205 G D -0.9786 Soluble
206 T D -0.6098 Soluble
207 Q D -0.9924 Soluble
208 T D -1.1042 Soluble
209 Y D 0.0000 Buried
210 I D -1.1150 Soluble
211 C D 0.0000 Buried
212 N D -1.3524 Soluble
213 V D 0.0000 Buried
214 N D -2.2818 Soluble
215 H D 0.0000 Buried
216 K D -2.9265 Soluble
217 P D -1.6907 Soluble
218 S D -1.8563 Soluble
219 N D -2.7070 Soluble
220 T D -2.2134 Soluble
221 K D -2.7272 Soluble
222 V D -1.3939 Soluble
223 D D -1.9626 Soluble
224 K D -1.7902 Soluble
225 K D -2.4985 Soluble
226 V D 0.0000 Buried
227 E D -2.5015 Soluble
228 P D -1.1226 Soluble
1 D C -2.2841 Soluble
2 I C 0.0000 Buried
3 Q C -2.3279 Soluble
4 M C 0.0000 Buried
5 T C -1.4508 Soluble
6 Q C 0.0000 Buried
7 S C -0.8561 Soluble
8 P C -0.7593 Soluble
9 S C -1.0423 Soluble
10 S C -0.9493 Soluble
11 L C -0.6312 Soluble
12 S C -0.9955 Soluble
13 A C 0.0000 Buried
14 S C -0.6449 Soluble
15 V C 0.4485 Aggregation-prone
16 G C -0.4689 Soluble
17 D C -1.5561 Soluble
18 R C -2.2497 Soluble
19 V C 0.0000 Buried
20 T C -0.6548 Soluble
21 I C 0.0000 Buried
22 T C -0.6860 Soluble
23 C C 0.0000 Buried
24 R C -2.5897 Soluble
25 A C 0.0000 Buried
26 S C -2.0917 Soluble
27 Q C -2.6053 Soluble
28 D C -2.5361 Soluble
29 V C 0.0000 Buried
36 N C -1.8837 Soluble
37 T C -0.8077 Soluble
38 A C -0.2359 Soluble
39 V C 0.0000 Buried
40 A C 0.0000 Buried
41 W C 0.0000 Buried
42 Y C 0.0000 Buried
43 Q C 0.0000 Buried
44 Q C 0.0000 Buried
45 K C -1.6529 Soluble
46 P C -1.4486 Soluble
47 G C -1.6594 Soluble
48 K C -2.5826 Soluble
49 A C -1.7275 Soluble
50 P C 0.0000 Buried
51 K C -1.4666 Soluble
52 L C 0.0000 Buried
53 L C 0.0000 Buried
54 I C 0.0000 Buried
55 Y C 1.1113 Aggregation-prone
56 S C 0.5534 Aggregation-prone
57 A C 0.0000 Buried
65 S C 0.6457 Aggregation-prone
66 F C 2.1610 Aggregation-prone
67 L C 1.5338 Aggregation-prone
68 Y C 0.7911 Aggregation-prone
69 S C 0.0668 Aggregation-prone
70 G C -0.3769 Soluble
71 V C -0.1910 Soluble
72 P C -0.2642 Soluble
74 S C -0.3920 Soluble
75 R C -0.7930 Soluble
76 F C 0.0000 Buried
77 S C 0.2217 Aggregation-prone
78 G C 0.0832 Aggregation-prone
79 S C -0.5621 Soluble
80 R C -1.5634 Soluble
83 S C -1.2183 Soluble
84 G C -1.6714 Soluble
85 T C -2.0810 Soluble
86 D C -1.9557 Soluble
87 F C 0.0000 Buried
88 T C -0.7419 Soluble
89 L C 0.0000 Buried
90 T C -0.6073 Soluble
91 I C 0.0000 Buried
92 S C -1.3286 Soluble
93 S C -0.9877 Soluble
94 L C 0.0000 Buried
95 Q C -0.8068 Soluble
96 P C -0.9056 Soluble
97 E C -1.8272 Soluble
98 D C 0.0000 Buried
99 F C -0.6880 Soluble
100 A C 0.0000 Buried
101 T C 0.0000 Buried
102 Y C 0.0000 Buried
103 Y C 0.0000 Buried
104 C C 0.0000 Buried
105 Q C 0.0000 Buried
106 Q C 0.0000 Buried
107 H C 0.0000 Buried
108 Y C 0.6939 Aggregation-prone
109 T C 0.2222 Aggregation-prone
114 T C -0.4405 Soluble
115 P C -0.9718 Soluble
116 P C 0.0000 Buried
117 T C -0.9014 Soluble
118 F C -0.6781 Soluble
119 G C -1.2540 Soluble
120 Q C -1.8802 Soluble
121 G C 0.0000 Buried
122 T C 0.0000 Buried
123 K C -1.8792 Soluble
124 V C 0.0000 Buried
125 E C -1.5635 Soluble
126 I C 0.0000 Buried
127 K C -1.3373 Soluble
128 R C -0.9338 Soluble
129 T C -0.0272 Soluble
130 V C 0.5777 Aggregation-prone
131 A C 0.2831 Aggregation-prone
132 A C -0.0485 Soluble
133 P C 0.0000 Buried
134 S C -0.1595 Soluble
135 V C 0.0000 Buried
136 F C -0.1105 Soluble
137 I C 0.0000 Buried
138 F C 0.0000 Buried
139 P C -0.5852 Soluble
140 P C 0.0000 Buried
141 S C -1.2551 Soluble
142 D C -2.4526 Soluble
143 E C -1.8906 Soluble
144 Q C 0.0000 Buried
145 L C -1.8236 Soluble
146 K C -2.4816 Soluble
147 S C -1.5615 Soluble
148 G C -1.3433 Soluble
149 T C -1.0379 Soluble
150 A C 0.0000 Buried
151 S C 0.0000 Buried
152 V C 0.0000 Buried
153 V C 0.0000 Buried
154 C C 0.0000 Buried
155 L C 0.0000 Buried
156 L C 0.0000 Buried
157 N C 0.0000 Buried
158 N C -0.6693 Soluble
159 F C 0.0000 Buried
160 Y C 0.0000 Buried
161 P C -1.6198 Soluble
162 R C -2.6683 Soluble
163 E C -3.1335 Soluble
164 A C -2.3296 Soluble
165 K C -2.2293 Soluble
166 V C -1.1837 Soluble
167 Q C -0.6308 Soluble
168 W C 0.0000 Buried
169 K C -0.6407 Soluble
170 V C -0.9958 Soluble
171 D C -2.1949 Soluble
172 N C -1.6646 Soluble
173 A C -0.4396 Soluble
174 L C 0.7003 Aggregation-prone
175 S C -0.4366 Soluble
176 G C -1.0852 Soluble
177 N C -1.7766 Soluble
178 S C -1.3465 Soluble
179 Q C -1.2397 Soluble
180 E C -1.3961 Soluble
181 S C -0.7332 Soluble
182 V C -0.7550 Soluble
183 T C -1.1729 Soluble
184 E C -2.2583 Soluble
185 Q C 0.0000 Buried
186 D C -1.8096 Soluble
187 S C -1.8519 Soluble
188 K C -2.1205 Soluble
189 D C -1.4346 Soluble
190 S C -1.6802 Soluble
191 T C 0.0000 Buried
192 Y C 0.0000 Buried
193 S C 0.0000 Buried
194 L C 0.0000 Buried
195 S C 0.0000 Buried
196 S C 0.0000 Buried
197 T C -0.6264 Soluble
198 L C 0.0000 Buried
199 T C -0.4092 Soluble
200 L C -0.8640 Soluble
201 S C -1.2403 Soluble
202 K C -2.3043 Soluble
203 A C -2.0769 Soluble
204 D C -2.9594 Soluble
205 Y C 0.0000 Buried
206 E C -3.5088 Soluble
207 K C -3.5471 Soluble
208 H C -2.8594 Soluble
209 K C -2.4395 Soluble
210 V C -1.0741 Soluble
211 Y C 0.0000 Buried
212 A C -0.6654 Soluble
213 C C 0.0000 Buried
214 E C -0.6763 Soluble
215 V C 0.0000 Buried
216 T C -1.0947 Soluble
217 H C 0.0000 Buried
218 Q C -1.4863 Soluble
219 G C -0.3472 Soluble
220 L C -0.2774 Soluble
221 S C -0.4862 Soluble
222 S C -0.4337 Soluble
223 P C -0.5073 Soluble
224 V C 0.0444 Aggregation-prone
225 T C -0.5038 Soluble
226 K C -1.0866 Soluble
227 S C -0.7857 Soluble
228 F C -0.9644 Soluble
229 N C -1.2939 Soluble
230 R C -2.0004 Soluble
231 G C -1.8639 Soluble
232 E C -1.8995 Soluble
233 C C -0.6065 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7348 3.6917 CSV PDB
4.5 -0.7789 3.6917 CSV PDB
5.0 -0.8296 3.6917 CSV PDB
5.5 -0.8775 3.6917 CSV PDB
6.0 -0.912 3.6917 CSV PDB
6.5 -0.9239 3.6917 CSV PDB
7.0 -0.9125 3.6917 CSV PDB
7.5 -0.8857 3.6917 CSV PDB
8.0 -0.8501 3.6917 CSV PDB
8.5 -0.8076 3.6916 CSV PDB
9.0 -0.7578 3.6914 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH