Cinrebafusp / 6bi0

A4DAB066_6bi0_H_L | Source PDB: 6bi0 | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.665
Max Score
1.8614
Min Score
-3.1479
Total Score
-281.28
Score CDR
-0.4606
Score non-CDR
-0.6905

Calculation Parameters

Chain Sequence(s) VQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCSRWGGDGFYAMDYWGQGTLVTVSSASTKGPSVFPLAPSTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPIQMTQSPSSLSASVGDRVTITCRASQDVNTAVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFGQGTKVEIKRTVAAPSVFIFPPSDQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGASQESVTEQDSKDSTYSLSSTLTLSKAAYEKHAVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.1479
Maximum Score 1.8614
Average Score -0.665
Total Score -281.2813

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V H 0.7856 Aggregation-prone
3 Q H -0.3601 Soluble
4 L H 0.0000 Buried
5 V H 0.2015 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.5606 Soluble
8 G H -0.8746 Soluble
9 G H -0.4574 Soluble
11 G H 0.0226 Aggregation-prone
12 L H 0.0694 Aggregation-prone
13 V H 0.0000 Buried
14 Q H -1.7116 Soluble
15 P H -1.8946 Soluble
16 G H -1.5868 Soluble
17 G H -1.2157 Soluble
18 S H -1.0773 Soluble
19 L H -1.0559 Soluble
20 R H -1.9108 Soluble
21 L H 0.0000 Buried
22 S H -0.4514 Soluble
23 C H 0.0000 Buried
24 A H -0.3658 Soluble
25 A H 0.0000 Buried
26 S H -0.4332 Soluble
27 G H -0.3609 Soluble
28 F H -0.9342 Soluble
29 N H -2.3645 Soluble
30 I H 0.0000 Buried
35 K H -3.0131 Soluble
36 D H -2.9147 Soluble
37 T H 0.0000 Buried
38 Y H -0.6598 Soluble
39 I H 0.0000 Buried
40 H H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.7814 Soluble
45 A H -1.1593 Soluble
46 P H -0.9603 Soluble
47 G H -1.4835 Soluble
48 K H -2.3774 Soluble
49 G H -1.6016 Soluble
50 L H -0.8714 Soluble
51 E H -1.1272 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 A H 0.0000 Buried
55 R H -0.6380 Soluble
56 I H 0.0000 Buried
57 Y H -0.3750 Soluble
58 P H -1.1621 Soluble
59 T H -1.3273 Soluble
62 N H -1.1203 Soluble
63 G H -0.4406 Soluble
64 Y H 0.5162 Aggregation-prone
65 T H -0.3837 Soluble
66 R H -1.7803 Soluble
67 Y H -1.7647 Soluble
68 A H -1.8488 Soluble
69 D H -2.7198 Soluble
70 S H -1.8057 Soluble
71 V H 0.0000 Buried
72 K H -2.8892 Soluble
74 G H -1.8002 Soluble
75 R H -1.5673 Soluble
76 F H 0.0000 Buried
77 T H -1.0583 Soluble
78 I H 0.0000 Buried
79 S H -0.1487 Soluble
80 A H -0.8295 Soluble
81 D H -1.5662 Soluble
82 T H -1.9885 Soluble
83 S H -1.6743 Soluble
84 K H -2.2692 Soluble
85 N H -1.9646 Soluble
86 T H -1.1552 Soluble
87 A H 0.0000 Buried
88 Y H -0.5082 Soluble
89 L H 0.0000 Buried
90 Q H -1.2318 Soluble
91 M H 0.0000 Buried
92 N H -1.3431 Soluble
93 S H -1.3571 Soluble
94 L H 0.0000 Buried
95 R H -2.9084 Soluble
96 A H -2.0157 Soluble
97 E H -2.4729 Soluble
98 D H 0.0000 Buried
99 T H -0.7851 Soluble
100 A H 0.0000 Buried
101 V H 0.2944 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 S H 0.0000 Buried
106 R H -0.4556 Soluble
107 W H 0.0000 Buried
108 G H 0.0000 Buried
109 G H -0.9518 Soluble
110 D H -1.7759 Soluble
111 G H 0.0000 Buried
112 F H 0.7724 Aggregation-prone
113 Y H 0.7443 Aggregation-prone
114 A H 0.0000 Buried
115 M H 0.0000 Buried
116 D H 0.0952 Aggregation-prone
117 Y H 0.1116 Aggregation-prone
118 W H -0.2059 Soluble
119 G H 0.0000 Buried
120 Q H -1.4729 Soluble
121 G H -0.7001 Soluble
122 T H -0.0679 Soluble
123 L H 0.3627 Aggregation-prone
124 V H 0.0000 Buried
125 T H -0.4366 Soluble
126 V H 0.0000 Buried
127 S H -0.8626 Soluble
128 S H -0.7104 Soluble
129 A H -0.4546 Soluble
130 S H -0.5419 Soluble
131 T H -0.7077 Soluble
132 K H -1.1610 Soluble
133 G H -1.3689 Soluble
134 P H 0.0000 Buried
135 S H -0.2202 Soluble
136 V H 0.0000 Buried
137 F H -0.2819 Soluble
138 P H -0.7654 Soluble
139 L H 0.0000 Buried
140 A H -0.6931 Soluble
141 P H 0.0000 Buried
142 S H -0.4932 Soluble
143 T H -0.2712 Soluble
144 A H 0.0000 Buried
145 A H 0.0000 Buried
146 L H 0.0000 Buried
147 G H 0.0000 Buried
148 C H 0.0000 Buried
149 L H 0.0000 Buried
150 V H 0.0000 Buried
151 K H 0.0000 Buried
152 D H -0.2757 Soluble
153 Y H 0.0000 Buried
154 F H 0.0000 Buried
155 P H 0.0000 Buried
156 E H -0.4466 Soluble
157 P H -0.7909 Soluble
158 V H -0.8403 Soluble
159 T H -0.7176 Soluble
160 V H -0.3003 Soluble
161 S H -0.4609 Soluble
162 W H 0.0000 Buried
163 N H -0.7356 Soluble
164 S H -0.6743 Soluble
165 G H -0.5819 Soluble
166 A H -0.2470 Soluble
167 L H 0.0204 Aggregation-prone
168 T H -0.1677 Soluble
169 S H -0.1569 Soluble
170 G H -0.1521 Soluble
171 V H 0.2255 Aggregation-prone
172 H H -0.3152 Soluble
173 T H 0.0294 Aggregation-prone
174 F H 0.0000 Buried
175 P H -0.2978 Soluble
176 A H 0.2717 Aggregation-prone
177 V H 0.6275 Aggregation-prone
178 L H 1.1671 Aggregation-prone
179 Q H 0.2847 Aggregation-prone
180 S H -0.0655 Soluble
181 S H -0.2022 Soluble
182 G H 0.0710 Aggregation-prone
183 L H 0.2067 Aggregation-prone
184 Y H 0.5140 Aggregation-prone
185 S H 0.0000 Buried
186 L H 0.0000 Buried
187 S H 0.0000 Buried
188 S H 0.0000 Buried
189 V H 0.0000 Buried
190 V H 0.0000 Buried
191 T H -0.1285 Soluble
192 V H 0.0000 Buried
193 P H -0.5463 Soluble
194 S H -0.4868 Soluble
195 S H -0.5776 Soluble
196 S H -0.6932 Soluble
197 L H -0.9391 Soluble
198 G H -1.2327 Soluble
199 T H -1.0548 Soluble
200 Q H -1.5864 Soluble
201 T H -1.1845 Soluble
202 Y H 0.0000 Buried
203 I H -0.9696 Soluble
204 C H 0.0000 Buried
205 N H -1.5635 Soluble
206 V H 0.0000 Buried
207 N H -2.3634 Soluble
208 H H 0.0000 Buried
209 K H -2.8805 Soluble
210 P H -1.6249 Soluble
211 S H -1.8828 Soluble
212 N H -2.6871 Soluble
213 T H -2.2243 Soluble
214 K H -2.9137 Soluble
215 V H -1.7562 Soluble
216 D H -2.5575 Soluble
217 K H -2.0973 Soluble
218 K H -2.0223 Soluble
219 V H 0.0000 Buried
220 E H -2.4563 Soluble
221 P H -1.2454 Soluble
2 I L -0.3959 Soluble
3 Q L -1.5427 Soluble
4 M L 0.0000 Buried
5 T L -1.3478 Soluble
6 Q L 0.0000 Buried
7 S L -0.9258 Soluble
8 P L -0.7203 Soluble
9 S L -1.0213 Soluble
10 S L -1.0510 Soluble
11 L L -0.6463 Soluble
12 S L -0.8808 Soluble
13 A L -0.8822 Soluble
14 S L -0.7437 Soluble
15 V L 0.0005 Aggregation-prone
16 G L -0.7669 Soluble
17 D L -1.9373 Soluble
18 R L -2.4074 Soluble
19 V L 0.0000 Buried
20 T L -0.6413 Soluble
21 I L 0.0000 Buried
22 T L -0.7669 Soluble
23 C L 0.0000 Buried
24 R L -2.8538 Soluble
25 A L 0.0000 Buried
26 S L -1.7931 Soluble
27 Q L -2.4971 Soluble
28 D L -2.7399 Soluble
29 V L 0.0000 Buried
36 N L -1.8452 Soluble
37 T L -0.8226 Soluble
38 A L 0.0900 Aggregation-prone
39 V L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.5955 Soluble
46 P L -1.3891 Soluble
47 G L -1.6252 Soluble
48 K L -2.5939 Soluble
49 A L -1.6591 Soluble
50 P L 0.0000 Buried
51 K L -1.7084 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.0000 Buried
56 S L 0.5909 Aggregation-prone
57 A L 0.0000 Buried
65 S L 0.5164 Aggregation-prone
66 F L 1.8614 Aggregation-prone
67 L L 1.2265 Aggregation-prone
68 Y L 0.4954 Aggregation-prone
69 S L -0.0588 Soluble
70 G L -0.4898 Soluble
71 V L 0.0000 Buried
72 P L -0.3800 Soluble
74 S L -0.4161 Soluble
75 R L -0.7158 Soluble
76 F L 0.0000 Buried
77 S L 0.0739 Aggregation-prone
78 G L 0.0021 Aggregation-prone
79 S L -0.5920 Soluble
80 R L -1.6374 Soluble
83 S L -1.2517 Soluble
84 G L -1.8763 Soluble
85 T L -2.2896 Soluble
86 D L -2.1069 Soluble
87 F L 0.0000 Buried
88 T L -0.7797 Soluble
89 L L 0.0000 Buried
90 T L -0.6078 Soluble
91 I L 0.0000 Buried
92 S L -1.3573 Soluble
93 S L -1.1132 Soluble
94 L L 0.0000 Buried
95 Q L -0.8823 Soluble
96 P L -1.3550 Soluble
97 E L -2.1048 Soluble
98 D L 0.0000 Buried
99 F L -1.2540 Soluble
100 A L 0.0000 Buried
101 T L -1.0615 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 H L 0.5368 Aggregation-prone
108 Y L 0.7389 Aggregation-prone
109 T L 0.6121 Aggregation-prone
114 T L -0.0858 Soluble
115 P L -0.4634 Soluble
116 P L 0.0000 Buried
117 T L -0.2790 Soluble
118 F L -0.4526 Soluble
119 G L 0.0000 Buried
120 Q L -1.8786 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -1.9692 Soluble
124 V L 0.0000 Buried
125 E L -1.2026 Soluble
126 I L 0.0000 Buried
127 K L -0.9927 Soluble
128 R L -0.6379 Soluble
129 T L -0.0017 Soluble
130 V L 0.3999 Aggregation-prone
131 A L -0.0084 Soluble
132 A L -0.1104 Soluble
133 P L 0.0000 Buried
134 S L -0.1311 Soluble
135 V L 0.0000 Buried
136 F L 0.2064 Aggregation-prone
137 I L 0.1942 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.5621 Soluble
140 P L -0.5708 Soluble
141 S L -1.0731 Soluble
142 D L -2.1715 Soluble
144 Q L 0.0000 Buried
145 L L -1.8330 Soluble
146 K L -2.3520 Soluble
147 S L -1.4445 Soluble
148 G L -1.1313 Soluble
149 T L -0.7751 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.8428 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.6005 Soluble
162 R L -2.6922 Soluble
163 E L -3.1479 Soluble
164 A L -2.3226 Soluble
165 K L -2.2594 Soluble
166 V L -1.0740 Soluble
167 Q L -0.5876 Soluble
168 W L 0.0000 Buried
169 K L -0.5822 Soluble
170 V L 0.0000 Buried
171 D L -1.6674 Soluble
172 N L -1.4134 Soluble
173 A L -0.3329 Soluble
174 L L 0.6151 Aggregation-prone
175 Q L -0.2360 Soluble
176 S L -0.3550 Soluble
177 G L -0.7511 Soluble
178 A L -0.4805 Soluble
179 S L -0.8695 Soluble
180 Q L -1.1373 Soluble
181 E L -1.5295 Soluble
182 S L -0.8241 Soluble
183 V L -0.7464 Soluble
184 T L -1.1717 Soluble
185 E L -2.1837 Soluble
186 Q L 0.0000 Buried
187 D L -2.2544 Soluble
188 S L -2.1621 Soluble
189 K L -2.5005 Soluble
190 D L -1.7190 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.5042 Soluble
199 L L 0.0000 Buried
200 T L -0.2385 Soluble
201 L L -0.3511 Soluble
202 S L -0.8055 Soluble
203 K L -1.9311 Soluble
204 A L -1.4621 Soluble
205 A L -1.4730 Soluble
206 Y L 0.0000 Buried
207 E L -3.0845 Soluble
208 K L -3.0165 Soluble
209 H L -2.0540 Soluble
210 A L -1.6876 Soluble
211 V L -0.7102 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.7399 Soluble
216 V L 0.0000 Buried
217 T L -1.2380 Soluble
218 H L 0.0000 Buried
219 Q L -1.7106 Soluble
220 G L -0.4264 Soluble
221 L L -0.2246 Soluble
222 S L -0.4523 Soluble
223 S L -0.4500 Soluble
224 P L -0.6074 Soluble
225 V L -0.1036 Soluble
226 T L -0.4764 Soluble
227 K L -0.6063 Soluble
228 S L -0.5438 Soluble
229 F L -0.7773 Soluble
230 N L -1.7187 Soluble
231 R L -2.1519 Soluble
232 G L -1.9839 Soluble
233 E L -2.4384 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6866 3.1833 CSV PDB
4.5 -0.7293 3.1833 CSV PDB
5.0 -0.7768 3.1833 CSV PDB
5.5 -0.8208 3.1833 CSV PDB
6.0 -0.8513 3.1833 CSV PDB
6.5 -0.8599 3.1833 CSV PDB
7.0 -0.8471 3.1833 CSV PDB
7.5 -0.8204 3.1833 CSV PDB
8.0 -0.7857 3.1832 CSV PDB
8.5 -0.7442 3.1832 CSV PDB
9.0 -0.6957 3.1829 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH