Tixagevimab / 7tbf

A4DAB270_7tbf_H_L | Source PDB: 7tbf | Chains: H, L | Done
95.26
Yes
10 Å
Yes
Avg A4D Score
-0.6824
Max Score
2.3455
Min Score
-3.3128
Total Score
-292.05
Score CDR
-0.333
Score non-CDR
-0.7296

Calculation Parameters

Chain Sequence(s) MQLVQSGPEVKKPGTSVKVSCKASGFTFTSSAVQWVRQARGQRLEWIGWIVVGSGNTNYAQKFQERVTITRDMSTSTAYMELSSLRSEDTAVYYCAAPYCSGGSCFDGFDIWGQGTMVTVSSASTKGPSVFPLAPSTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPEIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGFPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGNSPWTFGQGTKVEIRRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.3128
Maximum Score 2.3455
Average Score -0.6824
Total Score -292.053

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 M H 0.4582 Aggregation-prone
3 Q H -0.7981 Soluble
4 L H 0.0000 Buried
5 V H -0.0619 Soluble
6 Q H -0.5791 Soluble
7 S H -0.8986 Soluble
8 G H -0.8242 Soluble
9 P H -0.5017 Soluble
11 E H -0.6395 Soluble
12 V H 0.5049 Aggregation-prone
13 K H -0.7665 Soluble
14 K H -2.0774 Soluble
15 P H -1.9197 Soluble
16 G H -1.5279 Soluble
17 T H -1.1667 Soluble
18 S H -1.2513 Soluble
19 V H 0.0000 Buried
20 K H -2.1933 Soluble
21 V H 0.0000 Buried
22 S H -0.7094 Soluble
23 C H 0.0000 Buried
24 K H -1.0389 Soluble
25 A H 0.0000 Buried
26 S H -0.5439 Soluble
27 G H -0.2877 Soluble
28 F H 0.0077 Aggregation-prone
29 T H -0.1948 Soluble
30 F H 0.0000 Buried
35 T H -0.2632 Soluble
36 S H -0.2845 Soluble
37 S H 0.1151 Aggregation-prone
38 A H 0.0734 Aggregation-prone
39 V H 0.0000 Buried
40 Q H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H -0.6029 Soluble
44 Q H -1.2397 Soluble
45 A H -1.8465 Soluble
46 R H -2.5752 Soluble
47 G H -2.1757 Soluble
48 Q H -2.6708 Soluble
49 R H -2.2700 Soluble
50 L H 0.0000 Buried
51 E H -1.0557 Soluble
52 W H 0.0000 Buried
53 I H 0.0000 Buried
54 G H 0.0000 Buried
55 W H -0.0986 Soluble
56 I H 0.0000 Buried
57 V H -0.3718 Soluble
58 V H 0.0000 Buried
59 G H -0.6248 Soluble
62 S H -0.8020 Soluble
63 G H -1.0372 Soluble
64 N H -1.5877 Soluble
65 T H -1.0443 Soluble
66 N H -1.3097 Soluble
67 Y H -1.3497 Soluble
68 A H 0.0000 Buried
69 Q H -2.6262 Soluble
70 K H -2.8856 Soluble
71 F H 0.0000 Buried
72 Q H -2.8125 Soluble
74 E H -2.9112 Soluble
75 R H -2.0946 Soluble
76 V H 0.0000 Buried
77 T H -1.1047 Soluble
78 I H 0.0000 Buried
79 T H -0.8489 Soluble
80 R H -1.2941 Soluble
81 D H -1.0593 Soluble
82 M H -0.5813 Soluble
83 S H -0.4935 Soluble
84 T H -0.4703 Soluble
85 S H -0.7007 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.8951 Soluble
89 M H 0.0000 Buried
90 E H -1.7119 Soluble
91 L H 0.0000 Buried
92 S H -1.4164 Soluble
93 S H -1.1906 Soluble
94 L H 0.0000 Buried
95 R H -2.7970 Soluble
96 S H -2.2258 Soluble
97 E H -2.4629 Soluble
98 D H 0.0000 Buried
99 T H -0.9101 Soluble
100 A H 0.0000 Buried
101 V H -0.1155 Soluble
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 A H 0.0000 Buried
107 P H 0.1245 Aggregation-prone
108 Y H 0.4453 Aggregation-prone
109 C H 0.0000 Buried
110 S H -0.3152 Soluble
111 G H -0.7347 Soluble
111A G H -0.7953 Soluble
112B S H -0.4464 Soluble
112A C H 0.0783 Aggregation-prone
112 F H 0.0001 Aggregation-prone
113 D H -1.2781 Soluble
114 G H 0.0000 Buried
115 F H 0.0000 Buried
116 D H -0.6907 Soluble
117 I H 0.0000 Buried
118 W H -0.5247 Soluble
119 G H 0.0000 Buried
120 Q H -1.3830 Soluble
121 G H -0.7332 Soluble
122 T H 0.0000 Buried
123 M H 0.2317 Aggregation-prone
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -1.1041 Soluble
128 S H -0.7828 Soluble
129 A H -0.5126 Soluble
130 S H -0.4467 Soluble
131 T H -0.6903 Soluble
132 K H -0.9764 Soluble
133 G H -1.3479 Soluble
134 P H -0.6638 Soluble
135 S H -0.2635 Soluble
136 V H 0.0000 Buried
137 F H -0.4013 Soluble
138 P H -0.6769 Soluble
139 L H -0.0332 Soluble
140 A H -0.1096 Soluble
141 P H -0.0225 Soluble
142 S H -0.0661 Soluble
143 T H -0.1867 Soluble
144 A H -0.0348 Soluble
145 A H -0.0282 Soluble
146 L H 0.0000 Buried
147 G H 0.0000 Buried
148 C H 0.0000 Buried
149 L H 0.2154 Aggregation-prone
150 V H 0.0000 Buried
151 K H -0.3632 Soluble
152 D H -0.5577 Soluble
153 Y H 0.0000 Buried
154 F H 0.0000 Buried
155 P H -0.5292 Soluble
156 E H -0.5560 Soluble
157 P H -0.6465 Soluble
158 V H -0.3462 Soluble
159 T H -0.2802 Soluble
160 V H 0.1252 Aggregation-prone
161 S H -0.4408 Soluble
162 W H 0.0000 Buried
163 N H -0.7807 Soluble
164 S H -0.4791 Soluble
165 G H -0.5378 Soluble
166 A H 0.0617 Aggregation-prone
167 L H 0.5763 Aggregation-prone
168 T H 0.2935 Aggregation-prone
169 S H 0.1678 Aggregation-prone
170 G H -0.1471 Soluble
171 V H 0.3579 Aggregation-prone
172 H H -0.3043 Soluble
173 T H 0.1129 Aggregation-prone
174 F H 0.0000 Buried
175 P H -0.1363 Soluble
176 A H 0.3560 Aggregation-prone
177 V H 0.4851 Aggregation-prone
178 L H 1.2514 Aggregation-prone
179 Q H 0.2386 Aggregation-prone
180 S H -0.1222 Soluble
181 S H -0.2018 Soluble
182 G H 0.1181 Aggregation-prone
183 L H 0.0377 Aggregation-prone
184 Y H 0.0000 Buried
185 S H 0.1694 Aggregation-prone
186 L H 0.2661 Aggregation-prone
187 S H 0.0791 Aggregation-prone
188 S H 0.0000 Buried
189 V H 0.0000 Buried
190 V H 0.0000 Buried
191 T H -0.1452 Soluble
192 V H 0.0000 Buried
193 P H -0.4349 Soluble
194 S H -0.6006 Soluble
195 S H -0.3975 Soluble
196 S H -0.2942 Soluble
197 L H -0.0336 Soluble
198 G H -0.6033 Soluble
199 T H -0.7472 Soluble
200 Q H -1.1058 Soluble
201 T H -0.8664 Soluble
202 Y H -0.4058 Soluble
203 I H -0.9900 Soluble
204 C H 0.0000 Buried
205 N H -1.6888 Soluble
206 V H 0.0000 Buried
207 N H -1.6932 Soluble
208 H H 0.0000 Buried
209 K H -2.4272 Soluble
210 P H -1.5453 Soluble
211 S H -1.7727 Soluble
212 N H -2.1603 Soluble
213 T H -1.9256 Soluble
214 K H -2.6632 Soluble
215 V H -1.8448 Soluble
216 D H -2.8253 Soluble
217 K H -2.3416 Soluble
218 K H -2.4048 Soluble
219 V H 0.0000 Buried
220 E H -0.9542 Soluble
221 P H -0.5320 Soluble
1 E L 0.5229 Aggregation-prone
2 I L 2.3455 Aggregation-prone
3 V L 2.2972 Aggregation-prone
4 L L 0.7881 Aggregation-prone
5 T L -0.4735 Soluble
6 Q L 0.0000 Buried
7 S L -0.8566 Soluble
8 P L -0.5908 Soluble
9 G L -0.9748 Soluble
10 T L -0.6136 Soluble
11 L L -0.2012 Soluble
12 S L -0.4969 Soluble
13 L L -0.6299 Soluble
14 S L -1.1575 Soluble
15 P L -1.8645 Soluble
16 G L -2.3533 Soluble
17 E L -2.7757 Soluble
18 R L -2.9490 Soluble
19 A L 0.0000 Buried
20 T L -0.6495 Soluble
21 L L 0.0000 Buried
22 S L -0.8879 Soluble
23 C L 0.0000 Buried
24 R L -2.1006 Soluble
25 A L -0.6954 Soluble
26 S L -0.2965 Soluble
27 Q L -1.1054 Soluble
28 S L -1.2169 Soluble
29 V L 0.0000 Buried
30 S L -0.5568 Soluble
36 S L -0.3953 Soluble
37 S L -0.3083 Soluble
38 Y L 0.2059 Aggregation-prone
39 L L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L -0.7956 Soluble
44 Q L -1.2650 Soluble
45 K L -1.7018 Soluble
46 P L -1.3848 Soluble
47 G L -1.5137 Soluble
48 Q L -2.0882 Soluble
49 A L -1.4645 Soluble
50 P L 0.0000 Buried
51 R L -1.6777 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.4529 Soluble
56 G L -0.5532 Soluble
57 A L 0.0000 Buried
65 S L -0.7069 Soluble
66 S L -0.8902 Soluble
67 R L -1.3459 Soluble
68 A L 0.0000 Buried
69 T L -0.6300 Soluble
70 G L -1.0203 Soluble
71 F L 0.0000 Buried
72 P L -1.6088 Soluble
74 D L -2.6338 Soluble
75 R L -2.7106 Soluble
76 F L -1.4221 Soluble
77 S L -0.9860 Soluble
78 G L -0.6537 Soluble
79 S L -0.6892 Soluble
80 G L -1.1617 Soluble
83 S L -1.1640 Soluble
84 G L -1.3449 Soluble
85 T L -1.6027 Soluble
86 D L -1.8300 Soluble
87 F L 0.0000 Buried
88 T L -0.7751 Soluble
89 L L 0.0000 Buried
90 T L -0.8388 Soluble
91 I L 0.0000 Buried
92 S L -2.4551 Soluble
93 R L -3.0182 Soluble
94 L L 0.0000 Buried
95 E L -3.1503 Soluble
96 P L -2.2467 Soluble
97 E L -2.4953 Soluble
98 D L -1.7281 Soluble
99 F L -1.0179 Soluble
100 A L 0.0000 Buried
101 V L -0.7409 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 Y L 0.0000 Buried
108 G L -0.7822 Soluble
109 N L -1.1369 Soluble
114 S L -0.7380 Soluble
115 P L -0.4520 Soluble
116 W L 0.1687 Aggregation-prone
117 T L 0.5070 Aggregation-prone
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 Q L -1.5220 Soluble
121 G L -1.2906 Soluble
122 T L 0.0000 Buried
123 K L -1.3833 Soluble
124 V L 0.0000 Buried
125 E L 0.0000 Buried
126 I L -0.8824 Soluble
127 R L -1.8717 Soluble
128 R L -1.8730 Soluble
129 T L -0.8370 Soluble
130 V L -0.1306 Soluble
131 A L -0.1823 Soluble
132 A L -0.1408 Soluble
133 P L 0.0099 Aggregation-prone
134 S L 0.2798 Aggregation-prone
135 V L 0.0000 Buried
136 F L 0.5673 Aggregation-prone
137 I L 0.3238 Aggregation-prone
138 F L 0.5199 Aggregation-prone
139 P L 0.4862 Aggregation-prone
140 P L -0.6618 Soluble
141 S L -1.0784 Soluble
142 D L -2.4269 Soluble
143 E L -2.2210 Soluble
144 Q L 0.0000 Buried
145 L L -0.8538 Soluble
146 K L -2.0431 Soluble
147 S L -1.4074 Soluble
148 G L -1.3742 Soluble
149 T L -1.0695 Soluble
150 A L 0.0000 Buried
151 S L -0.3069 Soluble
152 V L 0.0000 Buried
153 V L 0.2031 Aggregation-prone
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L -0.1889 Soluble
158 N L -0.6522 Soluble
159 F L 0.0000 Buried
160 Y L -1.5727 Soluble
161 P L -1.8034 Soluble
162 R L -2.5051 Soluble
163 E L -3.0534 Soluble
164 A L -1.9032 Soluble
165 K L -1.0338 Soluble
166 V L -0.0830 Soluble
167 Q L -0.2141 Soluble
168 W L 0.1657 Aggregation-prone
169 K L -1.2257 Soluble
170 V L -0.5217 Soluble
171 D L -1.0922 Soluble
172 N L -1.9100 Soluble
173 A L -1.0112 Soluble
174 L L -0.5912 Soluble
175 Q L -0.7907 Soluble
176 S L -0.5279 Soluble
177 G L -0.7822 Soluble
178 N L -1.1907 Soluble
179 S L -1.0422 Soluble
180 Q L -1.7510 Soluble
181 E L -1.8157 Soluble
182 S L -0.2957 Soluble
183 V L 0.2926 Aggregation-prone
184 T L -0.4938 Soluble
185 E L -2.0115 Soluble
186 Q L -2.2484 Soluble
187 D L -3.1024 Soluble
188 S L -2.6502 Soluble
189 K L -3.1137 Soluble
190 D L -3.3128 Soluble
191 S L 0.0000 Buried
192 T L -2.0384 Soluble
193 Y L -0.9987 Soluble
194 S L 0.0000 Buried
195 L L -0.0880 Soluble
196 S L -0.2828 Soluble
197 S L -0.6459 Soluble
198 T L -0.5903 Soluble
199 L L -0.4872 Soluble
200 T L -0.3097 Soluble
201 L L -0.1959 Soluble
202 S L -1.1930 Soluble
203 K L -2.2030 Soluble
204 A L -1.8596 Soluble
205 D L -2.7145 Soluble
206 Y L -2.3443 Soluble
207 E L -3.2035 Soluble
208 K L -3.2858 Soluble
209 H L -2.1687 Soluble
210 K L -1.7022 Soluble
211 V L -0.8200 Soluble
212 Y L 0.0000 Buried
213 A L -0.1634 Soluble
214 C L -0.0030 Soluble
215 E L -0.1582 Soluble
216 V L 0.0000 Buried
217 T L -0.5301 Soluble
218 H L 0.0000 Buried
219 Q L -1.2757 Soluble
220 G L -0.2740 Soluble
221 L L 0.8989 Aggregation-prone
222 S L 0.1751 Aggregation-prone
223 S L 0.2241 Aggregation-prone
224 P L 0.7247 Aggregation-prone
225 V L 1.1284 Aggregation-prone
226 T L -0.0016 Soluble
227 K L -0.8007 Soluble
228 S L -0.1563 Soluble
229 F L 0.8216 Aggregation-prone
230 N L -0.8355 Soluble
231 R L -1.1725 Soluble
232 G L -1.2072 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Downloads
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6488 3.2046 CSV PDB
4.5 -0.692 3.1866 CSV PDB
5.0 -0.7448 3.1626 CSV PDB
5.5 -0.7965 3.1365 CSV PDB
6.0 -0.8353 3.1123 CSV PDB
6.5 -0.8522 3.0942 CSV PDB
7.0 -0.8457 3.0841 CSV PDB
7.5 -0.8225 3.0798 CSV PDB
8.0 -0.7898 3.1003 CSV PDB
8.5 -0.7507 3.2191 CSV PDB
9.0 -0.7063 3.3383 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH