Anbenitamab / 3bdy

A4DAB016_3bdy_H_L | Source PDB: 3bdy | Chains: H, L | Done
94.81
Yes
10 Å
Yes
Avg A4D Score
-0.7086
Max Score
1.6681
Min Score
-3.7135
Total Score
-306.13
Score CDR
-0.5726
Score non-CDR
-0.7268

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCSRWGGDGFYAMDYWGQGTLVTVSSASTKGPSVFPLAPSSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPDIQMTQSPSSLSASVGDRVTITCRASQDIPRSISGYVAWYQQKPGKAPKLLIYWGSYLYSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.7135
Maximum Score 1.6681
Average Score -0.7086
Total Score -306.1261

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.0505 Soluble
2 V H -1.2551 Soluble
3 Q H -1.1021 Soluble
4 L H 0.0000 Buried
5 V H 0.2206 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.6463 Soluble
8 G H -1.0327 Soluble
9 G H -0.6113 Soluble
11 G H -0.5538 Soluble
12 L H -0.1191 Soluble
13 V H 0.0000 Buried
14 Q H -1.7970 Soluble
15 P H -2.0067 Soluble
16 G H -1.5867 Soluble
17 G H -1.2652 Soluble
18 S H -1.2403 Soluble
19 L H -1.2633 Soluble
20 R H -2.2114 Soluble
21 L H 0.0000 Buried
22 S H -0.5650 Soluble
23 C H 0.0000 Buried
24 A H -0.2908 Soluble
25 A H 0.0000 Buried
26 S H -1.0185 Soluble
27 G H -1.4261 Soluble
28 F H -1.4308 Soluble
29 N H -2.3432 Soluble
30 I H 0.0000 Buried
35 K H -2.5588 Soluble
36 D H -2.8982 Soluble
37 T H 0.0000 Buried
38 Y H -0.2903 Soluble
39 I H 0.0000 Buried
40 H H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.8200 Soluble
45 A H -1.1940 Soluble
46 P H -0.9725 Soluble
47 G H -1.4649 Soluble
48 K H -2.3949 Soluble
49 G H -1.6388 Soluble
50 L H 0.0000 Buried
51 E H -1.1054 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 A H 0.0000 Buried
55 R H -0.3262 Soluble
56 I H 0.0000 Buried
57 Y H -0.2215 Soluble
58 P H 0.0000 Buried
59 T H -1.1439 Soluble
62 N H -1.0671 Soluble
63 G H -0.3230 Soluble
64 Y H 0.6244 Aggregation-prone
65 T H -0.1499 Soluble
66 R H -1.3149 Soluble
67 Y H -1.5744 Soluble
68 A H -1.7206 Soluble
69 D H -2.6433 Soluble
70 S H -1.7944 Soluble
71 V H 0.0000 Buried
72 K H -2.8058 Soluble
74 G H -1.7757 Soluble
75 R H -1.5009 Soluble
76 F H 0.0000 Buried
77 T H -1.0826 Soluble
78 I H 0.0000 Buried
79 S H -0.1787 Soluble
80 A H -0.9161 Soluble
81 D H -1.4698 Soluble
82 T H -1.5528 Soluble
83 S H -1.5667 Soluble
84 K H -2.2352 Soluble
85 N H -1.7901 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.6221 Soluble
89 L H 0.0000 Buried
90 Q H -1.3691 Soluble
91 M H 0.0000 Buried
92 N H -1.5844 Soluble
93 S H -1.4133 Soluble
94 L H 0.0000 Buried
95 R H -2.7874 Soluble
96 A H -1.9378 Soluble
97 E H -2.3846 Soluble
98 D H 0.0000 Buried
99 T H -0.8140 Soluble
100 A H 0.0000 Buried
101 V H 0.1230 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 S H 0.0000 Buried
106 R H 0.0000 Buried
107 W H 0.0000 Buried
108 G H -0.5279 Soluble
109 G H -1.4427 Soluble
110 D H -2.1630 Soluble
111 G H -1.0754 Soluble
112 F H -0.1564 Soluble
113 Y H 0.5209 Aggregation-prone
114 A H 0.0000 Buried
115 M H 0.0000 Buried
116 D H -0.1362 Soluble
117 Y H -0.2225 Soluble
118 W H -0.4765 Soluble
119 G H 0.0000 Buried
120 Q H -1.5328 Soluble
121 G H -0.7791 Soluble
122 T H -0.1537 Soluble
123 L H 0.1198 Aggregation-prone
124 V H 0.0000 Buried
125 T H -0.5188 Soluble
126 V H 0.0000 Buried
127 S H -0.9160 Soluble
128 S H -0.7057 Soluble
129 A H -0.4901 Soluble
130 S H -0.6310 Soluble
131 T H -0.7834 Soluble
132 K H -1.4962 Soluble
133 G H -1.5515 Soluble
134 P H -0.7065 Soluble
135 S H -0.3494 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.0328 Soluble
139 L H 0.0000 Buried
140 A H -0.5790 Soluble
141 P H 0.0000 Buried
142 S H -0.4005 Soluble
143 S H -0.3605 Soluble
144 G H -0.6750 Soluble
145 T H -0.5198 Soluble
146 A H -0.2773 Soluble
147 A H 0.0000 Buried
148 L H 0.0000 Buried
149 G H 0.0000 Buried
150 C H 0.0000 Buried
151 L H 0.0000 Buried
152 V H 0.0000 Buried
153 K H 0.0000 Buried
154 D H -0.4807 Soluble
155 Y H 0.0000 Buried
156 F H 0.0000 Buried
157 P H 0.0000 Buried
158 E H -0.4714 Soluble
159 P H -0.7128 Soluble
160 V H -0.6535 Soluble
161 T H -0.5716 Soluble
162 V H -0.3487 Soluble
163 S H -0.3836 Soluble
164 W H 0.0000 Buried
165 N H -0.7441 Soluble
166 S H -0.6397 Soluble
167 G H -0.4769 Soluble
168 A H -0.1899 Soluble
169 L H 0.0195 Aggregation-prone
170 T H -0.1628 Soluble
171 S H -0.2283 Soluble
172 G H -0.3169 Soluble
173 V H 0.1053 Aggregation-prone
174 H H -0.3154 Soluble
175 T H 0.0548 Aggregation-prone
176 F H 0.0000 Buried
177 P H -0.2533 Soluble
178 A H 0.2846 Aggregation-prone
179 V H 0.5835 Aggregation-prone
180 L H 1.3551 Aggregation-prone
181 Q H 0.2810 Aggregation-prone
182 S H -0.0286 Soluble
183 S H -0.1964 Soluble
184 G H 0.0963 Aggregation-prone
185 L H 0.1107 Aggregation-prone
186 Y H 0.4767 Aggregation-prone
187 S H 0.0000 Buried
188 L H 0.0000 Buried
189 S H 0.0000 Buried
190 S H 0.0000 Buried
191 V H 0.0000 Buried
192 V H 0.0000 Buried
193 T H -0.1885 Soluble
194 V H 0.0000 Buried
195 P H -0.4995 Soluble
196 S H -0.5643 Soluble
197 S H -0.5515 Soluble
198 S H -0.4988 Soluble
199 L H -0.4756 Soluble
200 G H -0.8097 Soluble
201 T H -0.6447 Soluble
202 Q H -0.9724 Soluble
203 T H -0.9541 Soluble
204 Y H 0.0000 Buried
205 I H -1.1342 Soluble
206 C H 0.0000 Buried
207 N H -1.5021 Soluble
208 V H 0.0000 Buried
209 N H -1.9624 Soluble
210 H H 0.0000 Buried
211 K H -2.8145 Soluble
212 P H -1.7127 Soluble
213 S H -1.9364 Soluble
214 N H -2.6440 Soluble
215 T H -2.1438 Soluble
216 K H -2.6940 Soluble
217 V H -1.6182 Soluble
218 D H -2.5673 Soluble
219 K H -2.0047 Soluble
220 K H -2.3982 Soluble
221 V H 0.0000 Buried
222 E H -1.4755 Soluble
223 P H -0.7488 Soluble
1 D L -2.2834 Soluble
2 I L 0.0000 Buried
3 Q L -2.2627 Soluble
4 M L 0.0000 Buried
5 T L -1.4002 Soluble
6 Q L 0.0000 Buried
7 S L -0.8529 Soluble
8 P L -0.5590 Soluble
9 S L -0.7459 Soluble
10 S L -0.5791 Soluble
11 L L -0.3580 Soluble
12 S L -0.6367 Soluble
13 A L -0.8359 Soluble
14 S L -0.7647 Soluble
15 V L -0.0469 Soluble
16 G L -0.8253 Soluble
17 D L -1.7043 Soluble
18 R L -2.3115 Soluble
19 V L 0.0000 Buried
20 T L -0.6463 Soluble
21 I L 0.0000 Buried
22 T L -0.7829 Soluble
23 C L 0.0000 Buried
24 R L -2.7374 Soluble
25 A L 0.0000 Buried
26 S L -2.2223 Soluble
27 Q L -2.5458 Soluble
28 D L -2.5992 Soluble
29 I L -1.5095 Soluble
30 P L -1.4944 Soluble
31 R L -2.2156 Soluble
34 S L -0.9584 Soluble
35 I L -0.3825 Soluble
36 S L -0.5004 Soluble
37 G L 0.2764 Aggregation-prone
38 Y L 0.0000 Buried
39 V L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L -0.9271 Soluble
44 Q L 0.0000 Buried
45 K L -1.7400 Soluble
46 P L -1.4161 Soluble
47 G L -1.7646 Soluble
48 K L -2.5590 Soluble
49 A L -1.6348 Soluble
50 P L 0.0000 Buried
51 K L -1.4942 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 1.0560 Aggregation-prone
56 W L 1.0210 Aggregation-prone
57 G L 0.0000 Buried
65 S L 0.6151 Aggregation-prone
66 Y L 1.6681 Aggregation-prone
67 L L 1.1648 Aggregation-prone
68 Y L 0.4595 Aggregation-prone
69 S L -0.0971 Soluble
70 G L -0.4944 Soluble
71 V L 0.0000 Buried
72 P L -0.3997 Soluble
74 S L -0.4270 Soluble
75 R L -0.7293 Soluble
76 F L 0.0000 Buried
77 S L -0.0306 Soluble
78 G L 0.0837 Aggregation-prone
79 S L -0.3508 Soluble
80 G L -1.0014 Soluble
83 S L -1.2369 Soluble
84 G L 0.0000 Buried
85 T L -1.9801 Soluble
86 D L -1.9148 Soluble
87 F L 0.0000 Buried
88 T L -0.6674 Soluble
89 L L 0.0000 Buried
90 T L -0.6211 Soluble
91 I L 0.0000 Buried
92 S L -1.3517 Soluble
93 S L -1.1064 Soluble
94 L L 0.0000 Buried
95 Q L -0.8291 Soluble
96 P L -1.4470 Soluble
97 E L -1.7882 Soluble
98 D L 0.0000 Buried
99 F L 0.0000 Buried
100 A L 0.0000 Buried
101 T L -0.7315 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 H L 0.5323 Aggregation-prone
108 Y L 0.7407 Aggregation-prone
109 T L 0.0594 Aggregation-prone
114 T L -0.4218 Soluble
115 P L -0.6516 Soluble
116 P L 0.0000 Buried
117 T L -0.7375 Soluble
118 F L -0.5266 Soluble
119 G L 0.0000 Buried
120 Q L -1.8442 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -1.1723 Soluble
124 V L 0.0000 Buried
125 E L -0.9253 Soluble
126 I L 0.0000 Buried
127 K L -0.9762 Soluble
128 R L -0.6222 Soluble
129 T L 0.0397 Aggregation-prone
130 V L 0.5604 Aggregation-prone
131 A L 0.2229 Aggregation-prone
132 A L -0.0994 Soluble
133 P L 0.0000 Buried
134 S L -0.1703 Soluble
135 V L 0.0000 Buried
136 F L 0.1021 Aggregation-prone
137 I L 0.1300 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.6838 Soluble
140 P L -0.7721 Soluble
141 S L -1.5895 Soluble
142 D L -2.8461 Soluble
143 E L -2.7621 Soluble
144 Q L 0.0000 Buried
145 L L -2.0901 Soluble
146 K L -2.6329 Soluble
147 S L -1.6349 Soluble
148 G L -0.9884 Soluble
149 T L -0.7624 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L -0.3683 Soluble
158 N L -0.8216 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.4199 Soluble
162 R L -2.5187 Soluble
163 E L -3.0272 Soluble
164 A L -2.2597 Soluble
165 K L -2.4552 Soluble
166 V L -1.1913 Soluble
167 Q L -0.7031 Soluble
168 W L 0.0000 Buried
169 K L -0.5766 Soluble
170 V L 0.0000 Buried
171 D L -1.6549 Soluble
172 N L -1.4137 Soluble
173 A L -0.2433 Soluble
174 L L 0.7335 Aggregation-prone
175 Q L -0.0998 Soluble
176 S L -0.4943 Soluble
177 G L -0.9139 Soluble
178 N L -0.7951 Soluble
179 S L -1.2793 Soluble
180 Q L -1.6919 Soluble
181 E L -1.9563 Soluble
182 S L -0.8881 Soluble
183 V L -0.3504 Soluble
184 T L -0.9277 Soluble
185 E L -2.1471 Soluble
186 Q L 0.0000 Buried
187 D L -2.0529 Soluble
188 S L -2.1741 Soluble
189 K L -2.5283 Soluble
190 D L -1.8138 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.7622 Soluble
199 L L 0.0000 Buried
200 T L -0.2936 Soluble
201 L L -0.5923 Soluble
202 S L -0.9710 Soluble
203 K L -1.9269 Soluble
204 A L -1.8265 Soluble
205 D L -2.5245 Soluble
206 Y L 0.0000 Buried
207 E L -3.7135 Soluble
208 K L -3.7132 Soluble
209 H L -3.2321 Soluble
210 K L -3.4451 Soluble
211 V L -1.7289 Soluble
212 Y L 0.0000 Buried
213 A L -0.9108 Soluble
214 C L 0.0000 Buried
215 E L -1.0991 Soluble
216 V L 0.0000 Buried
217 T L -1.2786 Soluble
218 H L 0.0000 Buried
219 Q L -1.7140 Soluble
220 G L -0.4429 Soluble
221 L L -0.2248 Soluble
222 S L -0.4663 Soluble
223 S L -0.4402 Soluble
224 P L -0.6173 Soluble
225 V L -0.1519 Soluble
226 T L -0.6233 Soluble
227 K L -0.8238 Soluble
228 S L -0.8097 Soluble
229 F L -1.3568 Soluble
230 N L -2.7236 Soluble
231 R L -3.3794 Soluble
232 G L -2.4711 Soluble
233 E L -2.5566 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7285 1.6727 CSV PDB
4.5 -0.7711 1.6499 CSV PDB
5.0 -0.8206 1.6291 CSV PDB
5.5 -0.8679 1.6323 CSV PDB
6.0 -0.9022 1.637 CSV PDB
6.5 -0.9152 1.6415 CSV PDB
7.0 -0.907 1.6443 CSV PDB
7.5 -0.8845 1.6455 CSV PDB
8.0 -0.8534 1.6457 CSV PDB
8.5 -0.8153 1.6453 CSV PDB
9.0 -0.7696 1.6717 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH