Tamtuvetmab / 1bfo

A4DAB255_1bfo_D_C | Source PDB: 1bfo | Chains: D, C | Done
96.93
Yes
10 Å
Yes
Avg A4D Score
-0.6421
Max Score
1.6142
Min Score
-3.1818
Total Score
-276.11
Score CDR
-0.9004
Score non-CDR
-0.6097

Calculation Parameters

Chain Sequence(s) EVKLLESGGGLVQPGGSMRLSCAGSGFTFTDFYMNWIRQPAGKAPEWLGFIRDKAKGYTTEYNPSVKGRFTISRDNTQNMLYLQMNTLRAEDTATYYCAREGHTAAPFDYWGQGVMVTVSSAQTTAPSVYPLAPGCGDTTSSTVTLGCLVKGYFPEPVTVTWNSGALSSDVHTFPAVLQSGLYTLTSSVTSSTWPSQTVTCNVAHPASSTKVDKKVDIKMTQSPSFLSASVGDRVTLNCKASQNIDKYLNWYQQKLGESPKLLIYNTNNLQTGIPSRFSGSGSGTDFTLTISSLQPEDVATYFCLQHISRPRTFGTGTKLELKRANAAPTVSIFPPSTEQLATGGASVVCLMNKFYPRDISVKWKIDGTERNGVLNSVTDQDSADSTYSMSSTLSLTKADYQSHNLYTCQVVHKTSSSPVVAKNFNRNEC input.pdb
Selected Chain(s) D (Heavy), C (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.1818
Maximum Score 1.6142
Average Score -0.6421
Total Score -276.1108

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E D -1.1323 Soluble
2 V D 0.2294 Aggregation-prone
3 K D -0.8540 Soluble
4 L D 0.0000 Buried
5 L D 0.1942 Aggregation-prone
6 E D 0.0000 Buried
7 S D -0.4947 Soluble
8 G D -0.8542 Soluble
9 G D -0.7918 Soluble
11 G D -0.1721 Soluble
12 L D -0.2953 Soluble
13 V D -0.8065 Soluble
14 Q D -1.7106 Soluble
15 P D -1.4950 Soluble
16 G D -1.3217 Soluble
17 G D -1.0473 Soluble
18 S D -1.2586 Soluble
19 M D -1.2465 Soluble
20 R D -2.1842 Soluble
21 L D 0.0000 Buried
22 S D -0.4442 Soluble
23 C D 0.0000 Buried
24 A D -0.2426 Soluble
25 G D -0.5580 Soluble
26 S D -0.5917 Soluble
27 G D -0.6343 Soluble
28 F D -0.6251 Soluble
29 T D -0.8242 Soluble
30 F D 0.0000 Buried
35 T D -1.4899 Soluble
36 D D -2.2539 Soluble
37 F D 0.0000 Buried
38 Y D -0.8200 Soluble
39 M D 0.0000 Buried
40 N D 0.0000 Buried
41 W D 0.0000 Buried
42 I D 0.0000 Buried
43 R D 0.0000 Buried
44 Q D -0.7314 Soluble
45 P D -1.0418 Soluble
46 A D -0.7067 Soluble
47 G D -1.2948 Soluble
48 K D -2.0273 Soluble
49 A D -1.0493 Soluble
50 P D 0.0000 Buried
51 E D -0.7244 Soluble
52 W D 0.0000 Buried
53 L D 0.0000 Buried
54 G D 0.0000 Buried
55 F D 0.0000 Buried
56 I D 0.0000 Buried
57 R D -1.5157 Soluble
58 D D -2.2456 Soluble
59 K D -2.8748 Soluble
60 A D -2.0869 Soluble
61 K D -2.6595 Soluble
62 G D -1.9025 Soluble
63 Y D -1.2120 Soluble
64 T D -0.8901 Soluble
65 T D -0.5017 Soluble
66 E D -0.8869 Soluble
67 Y D -0.9681 Soluble
68 N D 0.0000 Buried
69 P D -1.2384 Soluble
70 S D -0.9015 Soluble
71 V D 0.0000 Buried
72 K D -2.1665 Soluble
74 G D -1.5187 Soluble
75 R D -1.2734 Soluble
76 F D 0.0000 Buried
77 T D -0.9107 Soluble
78 I D 0.0000 Buried
79 S D -0.2985 Soluble
80 R D 0.0000 Buried
81 D D -1.2592 Soluble
82 N D -1.6770 Soluble
83 T D -1.1747 Soluble
84 Q D -1.7075 Soluble
85 N D -1.2725 Soluble
86 M D -0.6175 Soluble
87 L D 0.0000 Buried
88 Y D -0.5215 Soluble
89 L D 0.0000 Buried
90 Q D -1.2964 Soluble
91 M D 0.0000 Buried
92 N D -1.2905 Soluble
93 T D -1.0907 Soluble
94 L D 0.0000 Buried
95 R D -2.0891 Soluble
96 A D -1.6705 Soluble
97 E D -2.1372 Soluble
98 D D 0.0000 Buried
99 T D -0.7607 Soluble
100 A D 0.0000 Buried
101 T D -0.0230 Soluble
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 A D 0.0000 Buried
106 R D 0.0000 Buried
107 E D 0.0000 Buried
108 G D -0.8634 Soluble
109 H D -1.2065 Soluble
110 T D -0.6702 Soluble
112 A D -0.4094 Soluble
113 A D -0.2634 Soluble
114 P D 0.0000 Buried
115 F D 0.0000 Buried
116 D D -0.5296 Soluble
117 Y D -0.1992 Soluble
118 W D 0.0000 Buried
119 G D 0.0000 Buried
120 Q D -1.5385 Soluble
121 G D -0.6306 Soluble
122 V D 0.0262 Aggregation-prone
123 M D 0.1657 Aggregation-prone
124 V D 0.0000 Buried
125 T D 0.0000 Buried
126 V D 0.0000 Buried
127 S D -1.1917 Soluble
128 S D -0.9053 Soluble
129 A D -0.8385 Soluble
130 Q D -1.3453 Soluble
131 T D -0.6680 Soluble
132 T D -0.3345 Soluble
133 A D -0.4295 Soluble
134 P D -0.2385 Soluble
135 S D -0.2086 Soluble
136 V D 0.0000 Buried
137 Y D 0.0000 Buried
138 P D -0.7539 Soluble
139 L D 0.0000 Buried
140 A D -0.0739 Soluble
141 P D -0.2358 Soluble
142 G D -0.6771 Soluble
143 C D -1.2563 Soluble
144 G D -1.0725 Soluble
145 D D -1.1357 Soluble
146 T D -0.5109 Soluble
147 T D -0.4565 Soluble
148 S D -0.4203 Soluble
149 S D -0.3803 Soluble
150 T D -0.2739 Soluble
151 V D 0.0000 Buried
152 T D -0.2169 Soluble
153 L D 0.0000 Buried
154 G D 0.0000 Buried
155 C D 0.0000 Buried
156 L D 0.0000 Buried
157 V D 0.0000 Buried
158 K D -0.2752 Soluble
159 G D 0.0664 Aggregation-prone
160 Y D 0.0000 Buried
161 F D 0.0000 Buried
162 P D 0.0000 Buried
163 E D -0.3481 Soluble
164 P D -0.3288 Soluble
165 V D -0.1879 Soluble
166 T D -0.2088 Soluble
167 V D -0.1129 Soluble
168 T D -0.2382 Soluble
169 W D 0.0000 Buried
170 N D -0.8298 Soluble
171 S D -0.6176 Soluble
172 G D -0.3907 Soluble
173 A D -0.1852 Soluble
174 L D -0.2504 Soluble
175 S D -0.5502 Soluble
176 S D -0.7966 Soluble
177 D D -1.3050 Soluble
178 V D -0.2433 Soluble
179 H D 0.0000 Buried
180 T D 0.1220 Aggregation-prone
181 F D 0.0000 Buried
182 P D -0.2866 Soluble
183 A D 0.3376 Aggregation-prone
184 V D 0.8825 Aggregation-prone
185 L D 1.6142 Aggregation-prone
186 Q D 0.4626 Aggregation-prone
187 S D 0.0109 Aggregation-prone
188 G D 0.0842 Aggregation-prone
189 L D 0.5920 Aggregation-prone
190 Y D 0.7243 Aggregation-prone
191 T D 0.0000 Buried
192 L D 0.0000 Buried
193 T D 0.0000 Buried
194 S D 0.0000 Buried
195 S D 0.0000 Buried
196 V D 0.0000 Buried
197 T D -0.4127 Soluble
198 S D 0.0000 Buried
199 S D -0.4748 Soluble
200 T D -0.5561 Soluble
201 W D -0.3873 Soluble
202 P D -0.5890 Soluble
203 S D -0.9192 Soluble
204 Q D -1.3964 Soluble
205 T D -0.9662 Soluble
206 V D 0.0000 Buried
207 T D 0.0000 Buried
208 C D 0.0000 Buried
209 N D 0.0000 Buried
210 V D 0.0000 Buried
211 A D -0.9013 Soluble
212 H D 0.0000 Buried
213 P D -0.7442 Soluble
214 A D -0.4409 Soluble
215 S D -0.4833 Soluble
216 S D -0.7048 Soluble
217 T D -0.9063 Soluble
218 K D -2.0270 Soluble
219 V D -1.6784 Soluble
220 D D -2.6425 Soluble
221 K D -2.1813 Soluble
222 K D -2.0690 Soluble
223 V D -0.4229 Soluble
1 D C -2.6722 Soluble
2 I C 0.0000 Buried
3 K C -2.5905 Soluble
4 M C 0.0000 Buried
5 T C -1.0985 Soluble
6 Q C 0.0000 Buried
7 S C -0.3999 Soluble
8 P C -0.0004 Soluble
9 S C 0.1583 Aggregation-prone
10 F C 1.2130 Aggregation-prone
11 L C 0.4571 Aggregation-prone
12 S C -0.0355 Soluble
13 A C 0.0000 Buried
14 S C -0.7136 Soluble
15 V C -0.0085 Soluble
16 G C -0.8301 Soluble
17 D C -1.9280 Soluble
18 R C -2.3980 Soluble
19 V C 0.0000 Buried
20 T C -0.6050 Soluble
21 L C 0.0000 Buried
22 N C -1.1137 Soluble
23 C C 0.0000 Buried
24 K C -2.3015 Soluble
25 A C -2.0780 Soluble
26 S C -2.2368 Soluble
27 Q C -2.7844 Soluble
28 N C -2.7014 Soluble
29 I C 0.0000 Buried
36 D C -2.6057 Soluble
37 K C -2.3660 Soluble
38 Y C -0.4513 Soluble
39 L C 0.0000 Buried
40 N C 0.0000 Buried
41 W C 0.0000 Buried
42 Y C 0.0000 Buried
43 Q C 0.0000 Buried
44 Q C -1.0494 Soluble
45 K C -1.0767 Soluble
46 L C 0.1690 Aggregation-prone
47 G C -0.9194 Soluble
48 E C -2.1997 Soluble
49 S C -1.4936 Soluble
50 P C 0.0000 Buried
51 K C -1.2193 Soluble
52 L C 0.0000 Buried
53 L C 0.0000 Buried
54 I C 0.0000 Buried
55 Y C -0.5503 Soluble
56 N C -1.0465 Soluble
57 T C 0.0000 Buried
65 N C -1.8545 Soluble
66 N C -1.1064 Soluble
67 L C -0.2763 Soluble
68 Q C -0.4039 Soluble
69 T C -0.2671 Soluble
70 G C -0.3859 Soluble
71 I C -0.0532 Soluble
72 P C -0.2864 Soluble
74 S C -0.4057 Soluble
75 R C -0.8318 Soluble
76 F C 0.0000 Buried
77 S C -0.7443 Soluble
78 G C -0.9177 Soluble
79 S C -1.3870 Soluble
80 G C -1.6971 Soluble
83 S C -2.0908 Soluble
84 G C -2.4281 Soluble
85 T C -2.4519 Soluble
86 D C -2.5904 Soluble
87 F C 0.0000 Buried
88 T C -1.0065 Soluble
89 L C 0.0000 Buried
90 T C -0.6847 Soluble
91 I C 0.0000 Buried
92 S C -1.5157 Soluble
93 S C -1.2683 Soluble
94 L C 0.0000 Buried
95 Q C -0.9213 Soluble
96 P C -1.0792 Soluble
97 E C -1.8689 Soluble
98 D C 0.0000 Buried
99 V C -0.8626 Soluble
100 A C -0.7870 Soluble
101 T C -0.4535 Soluble
102 Y C 0.0000 Buried
103 F C 0.0000 Buried
104 C C 0.0000 Buried
105 L C 0.0000 Buried
106 Q C 0.0000 Buried
107 H C 0.1451 Aggregation-prone
108 I C 0.5568 Aggregation-prone
109 S C -0.2522 Soluble
114 R C -1.3756 Soluble
115 P C 0.0000 Buried
116 R C -0.9120 Soluble
117 T C -0.8924 Soluble
118 F C -0.2884 Soluble
119 G C 0.0000 Buried
120 T C -0.6442 Soluble
121 G C -0.6118 Soluble
122 T C 0.0000 Buried
123 K C -1.1311 Soluble
124 L C 0.0000 Buried
125 E C -0.5088 Soluble
126 L C 0.0000 Buried
127 K C -1.3164 Soluble
128 R C -1.3664 Soluble
129 A C -1.1622 Soluble
130 N C -1.5682 Soluble
131 A C -0.8256 Soluble
132 A C -0.4699 Soluble
133 P C -0.1792 Soluble
134 T C -0.1102 Soluble
135 V C 0.0000 Buried
136 S C -0.4416 Soluble
137 I C 0.0000 Buried
138 F C 0.0000 Buried
139 P C -0.2930 Soluble
140 P C -0.4526 Soluble
141 S C -0.5639 Soluble
142 T C -0.8772 Soluble
143 E C -1.7122 Soluble
144 Q C 0.0000 Buried
145 L C -0.7653 Soluble
146 A C -0.7252 Soluble
147 T C -0.6820 Soluble
148 G C -1.0043 Soluble
149 G C -0.8800 Soluble
150 A C 0.0000 Buried
151 S C 0.0000 Buried
152 V C 0.0000 Buried
153 V C 0.0000 Buried
154 C C 0.0000 Buried
155 L C 0.0000 Buried
156 M C 0.0000 Buried
157 N C 0.0000 Buried
158 K C -0.8267 Soluble
159 F C 0.0000 Buried
160 Y C 0.0000 Buried
161 P C -2.1159 Soluble
162 R C -2.5776 Soluble
163 D C -2.5521 Soluble
164 I C -1.2880 Soluble
165 S C -0.7877 Soluble
166 V C -0.0901 Soluble
167 K C -1.4602 Soluble
168 W C 0.0000 Buried
169 K C -2.2185 Soluble
170 I C 0.0000 Buried
171 D C -1.4657 Soluble
172 G C -1.4301 Soluble
173 T C -1.9293 Soluble
174 E C -3.1818 Soluble
175 R C -3.0064 Soluble
176 N C -2.3652 Soluble
177 G C -1.0431 Soluble
178 V C -0.6859 Soluble
179 L C -0.1327 Soluble
180 N C -0.6532 Soluble
181 S C -0.3510 Soluble
182 V C -0.3315 Soluble
183 T C -0.5759 Soluble
184 D C -1.2054 Soluble
185 Q C 0.0000 Buried
186 D C -1.0071 Soluble
187 S C -0.9842 Soluble
188 A C -0.8338 Soluble
189 D C -0.8223 Soluble
190 S C 0.0000 Buried
191 T C 0.0000 Buried
192 Y C 0.0000 Buried
193 S C 0.0000 Buried
194 M C 0.0000 Buried
195 S C 0.0000 Buried
196 S C 0.0000 Buried
197 T C 0.0000 Buried
198 L C 0.0000 Buried
199 S C -0.2034 Soluble
200 L C -0.6057 Soluble
201 T C -1.0683 Soluble
202 K C -1.5838 Soluble
203 A C -1.4591 Soluble
204 D C -2.1891 Soluble
205 Y C 0.0000 Buried
206 Q C -1.8668 Soluble
207 S C -1.4422 Soluble
208 H C -1.4073 Soluble
209 N C -1.4791 Soluble
210 L C -0.6034 Soluble
211 Y C 0.0000 Buried
212 T C 0.0000 Buried
213 C C 0.0000 Buried
214 Q C -0.5378 Soluble
215 V C 0.0000 Buried
216 V C -0.0079 Soluble
217 H C 0.0000 Buried
218 K C -2.0631 Soluble
219 T C -1.2536 Soluble
220 S C -0.7471 Soluble
221 S C -0.3307 Soluble
222 S C -0.3394 Soluble
223 P C 0.1233 Aggregation-prone
224 V C 0.6174 Aggregation-prone
225 V C 0.6084 Aggregation-prone
226 A C -0.3037 Soluble
227 K C -1.2754 Soluble
228 N C -1.4541 Soluble
229 F C 0.0000 Buried
230 N C -1.4007 Soluble
231 R C -1.9199 Soluble
232 N C -2.3452 Soluble
233 E C -2.4220 Soluble
234 C C -0.9717 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.5563 2.614 CSV PDB
4.5 -0.5981 2.6088 CSV PDB
5.0 -0.6459 2.6053 CSV PDB
5.5 -0.6918 2.6107 CSV PDB
6.0 -0.7257 2.64 CSV PDB
6.5 -0.7393 2.7142 CSV PDB
7.0 -0.7326 2.8396 CSV PDB
7.5 -0.713 2.9987 CSV PDB
8.0 -0.6866 3.1726 CSV PDB
8.5 -0.6543 3.3517 CSV PDB
9.0 -0.6149 3.5317 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH