Izalontamab / 5euk

A4DAB149_5euk_D_C | Source PDB: 5euk | Chains: D, C | Done
99.56
Yes
10 Å
Yes
Avg A4D Score
-0.6737
Max Score
1.9819
Min Score
-3.7331
Total Score
-286.99
Score CDR
-0.3334
Score non-CDR
-0.7149

Calculation Parameters

Chain Sequence(s) QVQLKQSGPGLVQPSQSLSITCTVSGFSLTNYGVHWVRQSPGKGLEWLGVIWSGGNTDYNTPFTSRLSINKDNSKSQVFFKMNSLQSNDTAIYYCARALTYYDYEFAYWGQGTLVTVSAASTKGPSVFPLAPGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKDILLTQSPVILSVSPGERVSFSCRASQSIGTNIHWYQQRTNGSPRLLIKYASESISGIPSRFSGSGSGTDFTLSINSVESEDIADYYCQQNNNWPTTFGAGTKLELKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGA input.pdb
Selected Chain(s) D (Heavy), C (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.7331
Maximum Score 1.9819
Average Score -0.6737
Total Score -286.9883

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q D -1.5294 Soluble
2 V D -0.9774 Soluble
3 Q D -1.9475 Soluble
4 L D 0.0000 Buried
5 K D -2.4307 Soluble
6 Q D 0.0000 Buried
7 S D -0.8610 Soluble
8 G D -1.1057 Soluble
9 P D -0.4036 Soluble
11 G D -0.3194 Soluble
12 L D -0.0006 Soluble
13 V D 0.0000 Buried
14 Q D -1.4029 Soluble
15 P D -1.5093 Soluble
16 S D -1.4619 Soluble
17 Q D -1.7405 Soluble
18 S D -1.4747 Soluble
19 L D 0.0000 Buried
20 S D -0.6031 Soluble
21 I D 0.0000 Buried
22 T D -0.5329 Soluble
23 C D 0.0000 Buried
24 T D -1.2205 Soluble
25 V D 0.0000 Buried
26 S D -1.3797 Soluble
27 G D -1.0502 Soluble
28 F D -0.5856 Soluble
29 S D -0.8973 Soluble
30 L D 0.0000 Buried
35 T D -0.9082 Soluble
36 N D -0.8411 Soluble
37 Y D 0.4733 Aggregation-prone
38 G D 0.0000 Buried
39 V D 0.0000 Buried
40 H D 0.0000 Buried
41 W D 0.0000 Buried
42 V D 0.0000 Buried
43 R D 0.0000 Buried
44 Q D -0.4270 Soluble
45 S D -0.9908 Soluble
46 P D -1.1324 Soluble
47 G D -1.6887 Soluble
48 K D -2.2026 Soluble
49 G D -1.1105 Soluble
50 L D 0.0000 Buried
51 E D 0.0000 Buried
52 W D 0.0000 Buried
53 L D 0.0000 Buried
54 G D 0.0000 Buried
55 V D 0.0000 Buried
56 I D 0.0000 Buried
57 W D -0.5174 Soluble
58 S D -0.6539 Soluble
59 G D -1.2346 Soluble
63 G D -1.5530 Soluble
64 N D -1.8557 Soluble
65 T D -1.3022 Soluble
66 D D -1.0089 Soluble
67 Y D -0.3614 Soluble
68 N D 0.0000 Buried
69 T D -0.1917 Soluble
70 P D -0.3592 Soluble
71 F D 0.0000 Buried
72 T D -0.4380 Soluble
74 S D -0.5154 Soluble
75 R D -0.8680 Soluble
76 L D 0.0000 Buried
77 S D -0.9042 Soluble
78 I D 0.0000 Buried
79 N D -1.7173 Soluble
80 K D -1.4988 Soluble
81 D D -1.8143 Soluble
82 N D -2.2415 Soluble
83 S D -1.7072 Soluble
84 K D -2.3841 Soluble
85 S D -1.6365 Soluble
86 Q D -1.5532 Soluble
87 V D 0.0000 Buried
88 F D -0.2518 Soluble
89 F D 0.0000 Buried
90 K D -1.3686 Soluble
91 M D 0.0000 Buried
92 N D -1.4732 Soluble
93 S D -1.3539 Soluble
94 L D 0.0000 Buried
95 Q D -2.1341 Soluble
96 S D -1.5657 Soluble
97 N D -2.0747 Soluble
98 D D 0.0000 Buried
99 T D -0.6304 Soluble
100 A D 0.0000 Buried
101 I D 0.5006 Aggregation-prone
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 A D 0.0000 Buried
106 R D 0.0000 Buried
107 A D 0.0000 Buried
108 L D 1.1068 Aggregation-prone
109 T D 1.2182 Aggregation-prone
110 Y D 1.8817 Aggregation-prone
111 Y D 1.9819 Aggregation-prone
112 D D 0.0000 Buried
113 Y D 0.6446 Aggregation-prone
114 E D 0.0000 Buried
115 F D 0.0000 Buried
116 A D 0.0988 Aggregation-prone
117 Y D 0.0043 Aggregation-prone
118 W D -0.6985 Soluble
119 G D 0.0000 Buried
120 Q D -1.8759 Soluble
121 G D -0.9066 Soluble
122 T D 0.0000 Buried
123 L D 0.2443 Aggregation-prone
124 V D 0.0000 Buried
125 T D -0.2866 Soluble
126 V D 0.0000 Buried
127 S D -0.6297 Soluble
128 A D -0.2936 Soluble
129 A D -0.2398 Soluble
130 S D -0.4310 Soluble
131 T D -0.6575 Soluble
132 K D -1.1076 Soluble
133 G D -1.1981 Soluble
134 P D 0.0000 Buried
135 S D -0.3798 Soluble
136 V D 0.0000 Buried
137 F D 0.0000 Buried
138 P D -1.4274 Soluble
139 L D 0.0000 Buried
140 A D -1.2758 Soluble
141 P D -0.9069 Soluble
142 G D -0.6431 Soluble
143 T D -0.4859 Soluble
144 A D 0.0000 Buried
145 A D 0.0000 Buried
146 L D 0.0000 Buried
147 G D 0.0000 Buried
148 C D 0.0000 Buried
149 L D 0.0000 Buried
150 V D 0.0000 Buried
151 K D 0.0000 Buried
152 D D -0.2573 Soluble
153 Y D 0.0000 Buried
154 F D 0.0000 Buried
155 P D 0.0000 Buried
156 E D -0.1569 Soluble
157 P D -0.3898 Soluble
158 V D -0.4772 Soluble
159 T D -0.4462 Soluble
160 V D -0.3574 Soluble
161 S D -0.3786 Soluble
162 W D 0.0000 Buried
163 N D -0.8011 Soluble
164 S D -0.6399 Soluble
165 G D -0.4440 Soluble
166 A D -0.2124 Soluble
167 L D -0.0265 Soluble
168 T D -0.1659 Soluble
169 S D -0.1940 Soluble
170 G D -0.2389 Soluble
171 V D 0.1389 Aggregation-prone
172 H D 0.0000 Buried
173 T D -0.1570 Soluble
174 F D 0.0000 Buried
175 P D -0.3649 Soluble
176 A D 0.2489 Aggregation-prone
177 V D 0.5314 Aggregation-prone
178 L D 1.3455 Aggregation-prone
179 Q D 0.3735 Aggregation-prone
180 S D -0.1026 Soluble
181 S D -0.1526 Soluble
182 G D 0.1485 Aggregation-prone
183 L D 0.2644 Aggregation-prone
184 Y D 0.5763 Aggregation-prone
185 S D 0.0000 Buried
186 L D 0.0000 Buried
187 S D 0.0000 Buried
188 S D 0.0000 Buried
189 V D 0.0000 Buried
190 V D 0.0000 Buried
191 T D -0.1329 Soluble
192 V D 0.0000 Buried
193 P D -0.4955 Soluble
194 S D -0.5495 Soluble
195 S D -0.6078 Soluble
196 S D -0.5797 Soluble
197 L D -0.9101 Soluble
198 G D -1.0044 Soluble
199 T D -0.6757 Soluble
200 Q D -1.1061 Soluble
201 T D -1.1316 Soluble
202 Y D 0.0000 Buried
203 I D -1.4623 Soluble
204 C D 0.0000 Buried
205 N D -1.4954 Soluble
206 V D 0.0000 Buried
207 N D -1.7677 Soluble
208 H D 0.0000 Buried
209 K D -2.2457 Soluble
210 P D -1.2872 Soluble
211 S D -1.5978 Soluble
212 N D -2.3692 Soluble
213 T D -1.8900 Soluble
214 K D -2.5785 Soluble
215 V D -1.6083 Soluble
216 D D -2.5679 Soluble
217 K D -2.3862 Soluble
218 R D -2.7377 Soluble
219 V D 0.0000 Buried
220 E D -2.9213 Soluble
221 P D -1.9492 Soluble
222 K D -2.4813 Soluble
1 D C -1.2592 Soluble
2 I C -0.4166 Soluble
3 L C 0.6607 Aggregation-prone
4 L C 0.0000 Buried
5 T C -0.3455 Soluble
6 Q C 0.0000 Buried
7 S C 0.0876 Aggregation-prone
8 P C 0.7827 Aggregation-prone
9 V C 1.8394 Aggregation-prone
10 I C 1.2461 Aggregation-prone
11 L C 0.4213 Aggregation-prone
12 S C -0.3009 Soluble
13 V C -0.9078 Soluble
14 S C -1.5374 Soluble
15 P C -1.7613 Soluble
16 G C -1.8453 Soluble
17 E C -2.5940 Soluble
18 R C -2.9590 Soluble
19 V C 0.0000 Buried
20 S C -0.9479 Soluble
21 F C 0.0000 Buried
22 S C -0.8159 Soluble
23 C C 0.0000 Buried
24 R C -2.5209 Soluble
25 A C 0.0000 Buried
26 S C -1.0083 Soluble
27 Q C -1.6471 Soluble
28 S C -1.6356 Soluble
29 I C 0.0000 Buried
36 G C -1.0114 Soluble
37 T C -0.9397 Soluble
38 N C -0.8494 Soluble
39 I C 0.0000 Buried
40 H C 0.0000 Buried
41 W C 0.0000 Buried
42 Y C 0.0000 Buried
43 Q C 0.0000 Buried
44 Q C -1.1941 Soluble
45 R C -1.5219 Soluble
46 T C -1.4430 Soluble
47 N C -1.6866 Soluble
48 G C -1.4884 Soluble
49 S C -1.1189 Soluble
50 P C 0.0000 Buried
51 R C -1.5580 Soluble
52 L C 0.0000 Buried
53 L C 0.0000 Buried
54 I C 0.0000 Buried
55 K C -0.7172 Soluble
56 Y C -0.5607 Soluble
57 A C 0.0000 Buried
65 S C -1.3002 Soluble
66 E C -2.0074 Soluble
67 S C -1.1999 Soluble
68 I C -0.5193 Soluble
69 S C -0.3554 Soluble
70 G C -0.4859 Soluble
71 I C -0.4006 Soluble
72 P C -0.3672 Soluble
74 S C -0.6156 Soluble
75 R C -1.1628 Soluble
76 F C 0.0000 Buried
77 S C -0.9622 Soluble
78 G C -0.7767 Soluble
79 S C -0.8977 Soluble
80 G C -1.1285 Soluble
83 S C -1.1299 Soluble
84 G C -1.3230 Soluble
85 T C -1.9690 Soluble
86 D C -2.5477 Soluble
87 F C 0.0000 Buried
88 T C -0.9096 Soluble
89 L C 0.0000 Buried
90 S C -0.9892 Soluble
91 I C 0.0000 Buried
92 N C -2.4236 Soluble
93 S C -2.1550 Soluble
94 V C 0.0000 Buried
95 E C -2.4600 Soluble
96 S C -2.3800 Soluble
97 E C -2.3252 Soluble
98 D C 0.0000 Buried
99 I C 0.0000 Buried
100 A C -1.1405 Soluble
101 D C 0.0000 Buried
102 Y C 0.0000 Buried
103 Y C 0.0000 Buried
104 C C 0.0000 Buried
105 Q C 0.0000 Buried
106 Q C 0.0000 Buried
107 N C -0.7063 Soluble
108 N C -1.3804 Soluble
109 N C -1.3453 Soluble
114 W C 0.0507 Aggregation-prone
115 P C 0.0000 Buried
116 T C 0.0000 Buried
117 T C -0.0206 Soluble
118 F C 0.2412 Aggregation-prone
119 G C 0.0000 Buried
120 A C -0.0055 Soluble
121 G C 0.1333 Aggregation-prone
122 T C 0.0000 Buried
123 K C -0.6597 Soluble
124 L C 0.0000 Buried
125 E C -1.3316 Soluble
126 L C 0.0000 Buried
127 K C -1.4122 Soluble
128 R C -1.0716 Soluble
129 T C -0.0532 Soluble
130 V C 0.3861 Aggregation-prone
131 A C 0.1494 Aggregation-prone
132 A C -0.0891 Soluble
133 P C 0.0000 Buried
134 S C -0.1696 Soluble
135 V C 0.0000 Buried
136 F C -0.0105 Soluble
137 I C 0.0253 Aggregation-prone
138 F C 0.0000 Buried
139 P C -0.4209 Soluble
140 P C 0.0000 Buried
141 S C -1.8673 Soluble
142 D C -3.1387 Soluble
143 E C -3.0153 Soluble
144 Q C 0.0000 Buried
145 L C -2.3309 Soluble
146 K C -2.7791 Soluble
147 S C -1.7355 Soluble
148 G C -1.2190 Soluble
149 T C -0.8621 Soluble
150 A C 0.0000 Buried
151 S C 0.0000 Buried
152 V C 0.0000 Buried
153 V C 0.0000 Buried
154 C C 0.0000 Buried
155 L C 0.0000 Buried
156 L C 0.0000 Buried
157 N C 0.0000 Buried
158 N C -0.9218 Soluble
159 F C 0.0000 Buried
160 Y C 0.0000 Buried
161 P C -1.6575 Soluble
162 R C -2.8029 Soluble
163 E C -3.1689 Soluble
164 A C -2.2899 Soluble
165 K C -2.4526 Soluble
166 V C -1.1440 Soluble
167 Q C -0.6202 Soluble
168 W C 0.0000 Buried
169 K C -0.6968 Soluble
170 V C 0.0000 Buried
171 D C -2.0152 Soluble
172 N C -1.6233 Soluble
173 A C -0.4154 Soluble
174 L C 0.4267 Aggregation-prone
175 Q C -0.2232 Soluble
176 S C -0.4399 Soluble
177 G C -0.8809 Soluble
178 N C -0.8067 Soluble
179 S C -1.1561 Soluble
180 Q C -1.4479 Soluble
181 E C -2.1926 Soluble
182 S C -1.0971 Soluble
183 V C -1.1196 Soluble
184 T C -1.1856 Soluble
185 E C -2.2824 Soluble
186 Q C 0.0000 Buried
187 D C -2.0162 Soluble
188 S C -2.3603 Soluble
189 K C -2.7122 Soluble
190 D C -1.9555 Soluble
191 S C 0.0000 Buried
192 T C 0.0000 Buried
193 Y C 0.0000 Buried
194 S C 0.0000 Buried
195 L C 0.0000 Buried
196 S C 0.0000 Buried
197 S C 0.0000 Buried
198 T C -0.7041 Soluble
199 L C 0.0000 Buried
200 T C -0.3333 Soluble
201 L C -0.6721 Soluble
202 S C -1.0808 Soluble
203 K C -2.1128 Soluble
204 A C -1.9789 Soluble
205 D C -2.6195 Soluble
206 Y C 0.0000 Buried
207 E C -3.7010 Soluble
208 K C -3.7331 Soluble
209 H C -3.0267 Soluble
210 K C -2.9114 Soluble
211 V C -1.1645 Soluble
212 Y C 0.0000 Buried
213 A C -0.7686 Soluble
214 C C 0.0000 Buried
215 E C -0.8893 Soluble
216 V C 0.0000 Buried
217 T C -1.1664 Soluble
218 H C 0.0000 Buried
219 Q C -1.7192 Soluble
220 G C -0.3729 Soluble
221 L C -0.1852 Soluble
222 S C -0.4245 Soluble
223 S C -0.3886 Soluble
224 P C -0.4845 Soluble
225 V C 0.0239 Aggregation-prone
226 T C -0.5206 Soluble
227 K C -0.8205 Soluble
228 S C -0.5145 Soluble
229 F C -0.4691 Soluble
230 N C -1.1873 Soluble
231 R C -1.8470 Soluble
232 G C -1.1896 Soluble
233 A C -0.5753 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6811 2.7762 CSV PDB
4.5 -0.7178 2.7371 CSV PDB
5.0 -0.7621 2.675 CSV PDB
5.5 -0.8054 2.5981 CSV PDB
6.0 -0.8369 2.5147 CSV PDB
6.5 -0.8475 2.429 CSV PDB
7.0 -0.8365 2.3426 CSV PDB
7.5 -0.8106 2.2562 CSV PDB
8.0 -0.7763 2.1907 CSV PDB
8.5 -0.7365 2.1877 CSV PDB
9.0 -0.692 2.2591 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH