Itanistomig / 5ggt

A4DAB146_5ggt_H_L | Source PDB: 5ggt | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6982
Max Score
2.3561
Min Score
-3.5938
Total Score
-297.41
Score CDR
-0.1141
Score non-CDR
-0.7741

Calculation Parameters

Chain Sequence(s) VQLVQSGAEVKKPGSSVKVSCKTSGDTFSTYAISWVRQAPGQGLEWMGGIIPIFGKAHYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYFCARKFHFVSGSPFGMDVWGQGTTVTVSSASTKGPSVFPLAPSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPEIVLTQSPATLSLSPGERATLSCRASQSVSSYLAWYQQKPGQAPRLLIYDASNRATGIPARFSGSGSGTDFTLTISSLEPEDFAVYYCQQRSNWPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNR input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.5938
Maximum Score 2.3561
Average Score -0.6982
Total Score -297.4149

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V H 0.6314 Aggregation-prone
3 Q H -0.7583 Soluble
4 L H 0.0000 Buried
5 V H -0.5000 Soluble
6 Q H -0.5578 Soluble
7 S H -0.8486 Soluble
8 G H -0.7791 Soluble
9 A H -0.4371 Soluble
11 E H -0.9510 Soluble
12 V H -0.5578 Soluble
13 K H -1.6193 Soluble
14 K H -2.4622 Soluble
15 P H -2.2826 Soluble
16 G H -1.6713 Soluble
17 S H -1.3388 Soluble
18 S H -1.4758 Soluble
19 V H 0.0000 Buried
20 K H -2.0059 Soluble
21 V H 0.0000 Buried
22 S H -0.7118 Soluble
23 C H 0.0000 Buried
24 K H -1.4494 Soluble
25 T H -0.9708 Soluble
26 S H -0.7961 Soluble
27 G H -0.7554 Soluble
28 D H -1.2679 Soluble
29 T H -0.7571 Soluble
30 F H 0.0000 Buried
35 S H 0.1843 Aggregation-prone
36 T H 0.5152 Aggregation-prone
37 Y H 0.3450 Aggregation-prone
38 A H 0.0000 Buried
39 I H 0.0000 Buried
40 S H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H -0.5156 Soluble
44 Q H -0.6632 Soluble
45 A H -1.0812 Soluble
46 P H -1.1023 Soluble
47 G H -1.2982 Soluble
48 Q H -1.8384 Soluble
49 G H -1.2830 Soluble
50 L H 0.0000 Buried
51 E H -0.9150 Soluble
52 W H 0.0000 Buried
53 M H 0.0000 Buried
54 G H 0.0000 Buried
55 G H 0.0000 Buried
56 I H 0.0000 Buried
57 I H 0.8884 Aggregation-prone
58 P H 0.0000 Buried
59 I H 2.3104 Aggregation-prone
62 F H 2.3561 Aggregation-prone
63 G H 0.3184 Aggregation-prone
64 K H -1.0246 Soluble
65 A H -0.7142 Soluble
66 H H -1.0338 Soluble
67 Y H -0.9404 Soluble
68 A H -1.6354 Soluble
69 Q H -2.7166 Soluble
70 K H -2.8147 Soluble
71 F H 0.0000 Buried
72 Q H -2.5709 Soluble
74 G H -1.8917 Soluble
75 R H -1.6505 Soluble
76 V H 0.0000 Buried
77 T H -1.0322 Soluble
78 I H 0.0000 Buried
79 T H -0.6929 Soluble
80 A H -0.6314 Soluble
81 D H -1.4907 Soluble
82 E H -1.9081 Soluble
83 S H -1.3518 Soluble
84 T H -1.1837 Soluble
85 S H -1.4834 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.5620 Soluble
89 M H 0.0000 Buried
90 E H -1.3923 Soluble
91 L H 0.0000 Buried
92 S H -1.2837 Soluble
93 S H -1.4332 Soluble
94 L H 0.0000 Buried
95 R H -3.1288 Soluble
96 S H -2.4276 Soluble
97 E H -2.5711 Soluble
98 D H -1.7651 Soluble
99 T H -1.1021 Soluble
100 A H 0.0000 Buried
101 V H 0.1584 Aggregation-prone
102 Y H 0.0000 Buried
103 F H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 K H 0.0352 Aggregation-prone
108 F H 0.6254 Aggregation-prone
109 H H -0.1765 Soluble
110 F H 0.8709 Aggregation-prone
111 V H 0.0000 Buried
111A S H 0.0363 Aggregation-prone
112B G H -0.2775 Soluble
112A S H -0.3065 Soluble
112 P H -0.2809 Soluble
113 F H 0.0000 Buried
114 G H 0.0000 Buried
115 M H 0.0000 Buried
116 D H 0.0000 Buried
117 V H -0.0127 Soluble
118 W H 0.0000 Buried
119 G H 0.0000 Buried
120 Q H -1.4737 Soluble
121 G H -0.8066 Soluble
122 T H 0.0000 Buried
123 T H -0.1978 Soluble
124 V H 0.0000 Buried
125 T H -0.9817 Soluble
126 V H 0.0000 Buried
127 S H -1.4546 Soluble
128 S H -1.1792 Soluble
129 A H -0.7155 Soluble
130 S H -0.8093 Soluble
131 T H -0.9962 Soluble
132 K H -1.7085 Soluble
133 G H -1.7451 Soluble
134 P H 0.0000 Buried
135 S H -0.4574 Soluble
136 V H 0.0000 Buried
137 F H -0.6841 Soluble
138 P H -0.9952 Soluble
139 L H 0.0000 Buried
140 A H -0.6222 Soluble
141 P H -0.5606 Soluble
142 S H -0.3685 Soluble
143 G H -0.6449 Soluble
144 T H -0.5319 Soluble
145 A H 0.0000 Buried
146 A H 0.0000 Buried
147 L H 0.0000 Buried
148 G H 0.0000 Buried
149 C H 0.0000 Buried
150 L H 0.0000 Buried
151 V H 0.0000 Buried
152 K H 0.0000 Buried
153 D H -0.7865 Soluble
154 Y H 0.0000 Buried
155 F H 0.0000 Buried
156 P H 0.0000 Buried
157 E H -0.9401 Soluble
158 P H -1.1046 Soluble
159 V H 0.0000 Buried
160 T H -0.7776 Soluble
161 V H -0.3296 Soluble
162 S H -0.5455 Soluble
163 W H 0.0000 Buried
164 N H -0.7904 Soluble
165 S H -0.7342 Soluble
166 G H -0.6413 Soluble
167 A H -0.2678 Soluble
168 L H -0.0260 Soluble
169 T H -0.2068 Soluble
170 S H -0.2212 Soluble
171 G H -0.2909 Soluble
172 V H 0.1959 Aggregation-prone
173 H H 0.0000 Buried
174 T H -0.0881 Soluble
175 F H 0.0000 Buried
176 P H -0.1412 Soluble
177 A H 0.3525 Aggregation-prone
178 V H 0.6004 Aggregation-prone
179 L H 1.1518 Aggregation-prone
180 Q H 0.2265 Aggregation-prone
181 S H -0.0581 Soluble
182 S H -0.2526 Soluble
183 G H -0.0792 Soluble
184 L H -0.1748 Soluble
185 Y H 0.2024 Aggregation-prone
186 S H 0.0000 Buried
187 L H 0.0000 Buried
188 S H 0.0000 Buried
189 S H 0.0000 Buried
190 V H 0.0000 Buried
191 V H 0.0000 Buried
192 T H -0.2343 Soluble
193 V H 0.0000 Buried
194 P H -0.4693 Soluble
195 S H -0.5348 Soluble
196 S H -0.6200 Soluble
197 S H 0.0000 Buried
198 L H -0.7729 Soluble
199 G H -0.9715 Soluble
200 T H -0.8555 Soluble
201 Q H -1.2800 Soluble
202 T H -1.1372 Soluble
203 Y H 0.0000 Buried
204 I H -1.3518 Soluble
205 C H 0.0000 Buried
206 N H -1.7696 Soluble
207 V H 0.0000 Buried
208 N H -2.1124 Soluble
209 H H 0.0000 Buried
210 K H -2.8211 Soluble
211 P H -1.7264 Soluble
212 S H -2.0391 Soluble
213 N H -2.6167 Soluble
214 T H -2.2055 Soluble
215 K H -2.8183 Soluble
216 V H -1.8980 Soluble
217 D H -2.7249 Soluble
218 K H -2.1941 Soluble
219 K H -2.5629 Soluble
220 V H 0.0000 Buried
221 E H -2.3558 Soluble
222 P H -1.1807 Soluble
1 E L -1.7622 Soluble
2 I L 0.0000 Buried
3 V L 0.4842 Aggregation-prone
4 L L 0.0000 Buried
5 T L -0.2593 Soluble
6 Q L 0.0000 Buried
7 S L -0.5827 Soluble
8 P L -0.4792 Soluble
9 A L -0.5871 Soluble
10 T L -0.6225 Soluble
11 L L -0.4792 Soluble
12 S L -0.6457 Soluble
13 L L -1.0127 Soluble
14 S L -1.4926 Soluble
15 P L -1.7802 Soluble
16 G L -1.8187 Soluble
17 E L -2.4800 Soluble
18 R L -2.7367 Soluble
19 A L 0.0000 Buried
20 T L -0.5236 Soluble
21 L L 0.0000 Buried
22 S L -0.6993 Soluble
23 C L 0.0000 Buried
24 R L -1.7537 Soluble
25 A L 0.0000 Buried
26 S L -0.9895 Soluble
27 Q L -1.7157 Soluble
28 S L -1.4135 Soluble
29 V L 0.0000 Buried
36 S L -0.6853 Soluble
37 S L -0.4637 Soluble
38 Y L -0.0204 Soluble
39 L L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L -1.3069 Soluble
44 Q L 0.0000 Buried
45 K L -2.7563 Soluble
46 P L -1.8228 Soluble
47 G L -1.6596 Soluble
48 Q L -2.4259 Soluble
49 A L -1.5786 Soluble
50 P L 0.0000 Buried
51 R L -1.6991 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.5233 Soluble
56 D L -0.7298 Soluble
57 A L 0.0000 Buried
65 S L -1.2271 Soluble
66 N L -1.8713 Soluble
67 R L -1.6568 Soluble
68 A L -1.0113 Soluble
69 T L -0.6005 Soluble
70 G L -0.5377 Soluble
71 I L -0.4792 Soluble
72 P L -0.3152 Soluble
74 A L -0.3189 Soluble
75 R L -0.7343 Soluble
76 F L 0.0000 Buried
77 S L -0.7056 Soluble
78 G L 0.0000 Buried
79 S L -0.9686 Soluble
80 G L -1.1490 Soluble
83 S L -1.0916 Soluble
84 G L -1.2147 Soluble
85 T L -1.8233 Soluble
86 D L -2.5556 Soluble
87 F L 0.0000 Buried
88 T L -0.7551 Soluble
89 L L 0.0000 Buried
90 T L -0.5880 Soluble
91 I L 0.0000 Buried
92 S L -1.4111 Soluble
93 S L -1.7571 Soluble
94 L L 0.0000 Buried
95 E L -2.1055 Soluble
96 P L -1.8254 Soluble
97 E L -2.4503 Soluble
98 D L 0.0000 Buried
99 F L 0.0000 Buried
100 A L 0.0000 Buried
101 V L 0.0000 Buried
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 R L 0.0000 Buried
108 S L -0.6257 Soluble
114 N L -1.2256 Soluble
115 W L -0.2198 Soluble
116 P L 0.0000 Buried
117 T L -0.1787 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 Q L -1.2009 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -1.9788 Soluble
124 V L 0.0000 Buried
125 E L -1.0295 Soluble
126 I L -0.9905 Soluble
127 K L -1.2991 Soluble
128 R L -0.9808 Soluble
129 T L -0.0000 Buried
130 V L 0.6655 Aggregation-prone
131 A L 0.2043 Aggregation-prone
132 A L -0.1191 Soluble
133 P L 0.0000 Buried
134 S L -0.2168 Soluble
135 V L 0.0000 Buried
136 F L 0.0000 Buried
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.6116 Soluble
140 P L 0.0000 Buried
141 S L -1.2029 Soluble
142 D L -2.3800 Soluble
143 E L -1.8018 Soluble
144 Q L 0.0000 Buried
145 L L -1.9292 Soluble
146 K L -2.5116 Soluble
147 S L -1.5719 Soluble
148 G L -1.4244 Soluble
149 T L -1.2009 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.8647 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.2946 Soluble
162 R L -1.9893 Soluble
163 E L -2.7227 Soluble
164 A L -2.0395 Soluble
165 K L -2.2340 Soluble
166 V L -1.1324 Soluble
167 Q L -0.6349 Soluble
168 W L 0.0000 Buried
169 K L -0.3030 Soluble
170 V L 0.0000 Buried
171 D L -1.3246 Soluble
172 N L -1.0720 Soluble
173 A L -0.0968 Soluble
174 L L 0.7173 Aggregation-prone
175 Q L -0.2125 Soluble
176 S L -0.4028 Soluble
177 G L -0.8302 Soluble
178 N L -0.9619 Soluble
179 S L -1.2602 Soluble
180 Q L -1.5211 Soluble
181 E L -1.9188 Soluble
182 S L 0.0000 Buried
183 V L -0.6157 Soluble
184 T L -0.8953 Soluble
185 E L -2.1116 Soluble
186 Q L -1.7795 Soluble
187 D L -1.9389 Soluble
188 S L -1.9598 Soluble
189 K L -2.3334 Soluble
190 D L -1.8308 Soluble
191 S L -1.7922 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.7463 Soluble
199 L L 0.0000 Buried
200 T L -0.4096 Soluble
201 L L -0.7059 Soluble
202 S L -1.4114 Soluble
203 K L -2.2998 Soluble
204 A L -2.0371 Soluble
205 D L -2.8462 Soluble
206 Y L 0.0000 Buried
207 E L -3.3715 Soluble
208 K L -3.5938 Soluble
209 H L -3.0988 Soluble
210 K L -3.0763 Soluble
211 V L -1.4292 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.7512 Soluble
216 V L 0.0000 Buried
217 T L -1.2764 Soluble
218 H L 0.0000 Buried
219 Q L -1.3130 Soluble
220 G L -0.4037 Soluble
221 L L -0.2846 Soluble
222 S L -0.5442 Soluble
223 S L -0.4481 Soluble
224 P L -0.5627 Soluble
225 V L -0.0487 Soluble
226 T L -0.4816 Soluble
227 K L -0.7642 Soluble
228 S L -0.7167 Soluble
229 F L -0.9073 Soluble
230 N L -2.1010 Soluble
231 R L -2.6497 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Downloads
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7035 4.6672 CSV PDB
4.5 -0.7454 4.6686 CSV PDB
5.0 -0.7941 4.673 CSV PDB
5.5 -0.8393 4.6858 CSV PDB
6.0 -0.8697 4.7198 CSV PDB
6.5 -0.8763 4.7912 CSV PDB
7.0 -0.8579 4.9019 CSV PDB
7.5 -0.8228 5.0371 CSV PDB
8.0 -0.7789 5.1827 CSV PDB
8.5 -0.7289 5.3316 CSV PDB
9.0 -0.6732 5.4811 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH