Cinrebafusp / 7t99

A4DAB066_7t99_E_F | Source PDB: 7t99 | Chains: E, F | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6745
Max Score
2.1175
Min Score
-3.5128
Total Score
-290.71
Score CDR
-0.468
Score non-CDR
-0.6998

Calculation Parameters

Chain Sequence(s) VQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCSRWGGDGFYAMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKDIQMTQSPSSLSASVGDRVTITCRASQDVNTAVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLCSPVTKCFNRG input.pdb
Selected Chain(s) E (Heavy), F (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.5128
Maximum Score 2.1175
Average Score -0.6745
Total Score -290.707

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V E 1.3440 Aggregation-prone
3 Q E 0.3361 Aggregation-prone
4 L E 0.0000 Buried
5 V E 0.4822 Aggregation-prone
6 E E 0.0000 Buried
7 S E -0.3837 Soluble
8 G E -0.6860 Soluble
9 G E -0.2516 Soluble
11 G E -0.2154 Soluble
12 L E 0.0511 Aggregation-prone
13 V E -0.5192 Soluble
14 Q E -1.5108 Soluble
15 P E -1.5128 Soluble
16 G E -1.3094 Soluble
17 G E -1.0875 Soluble
18 S E -1.0264 Soluble
19 L E -0.7201 Soluble
20 R E -1.2704 Soluble
21 L E 0.0000 Buried
22 S E -0.2533 Soluble
23 C E 0.0000 Buried
24 A E -0.2779 Soluble
25 A E -0.1374 Soluble
26 S E 0.1033 Aggregation-prone
27 G E 0.1776 Aggregation-prone
28 F E -0.1694 Soluble
29 N E -1.1716 Soluble
30 I E 0.0000 Buried
35 K E -1.8675 Soluble
36 D E -2.4159 Soluble
37 T E 0.0000 Buried
38 Y E -0.2748 Soluble
39 I E 0.0000 Buried
40 H E 0.0000 Buried
41 W E 0.0000 Buried
42 V E 0.0000 Buried
43 R E 0.0000 Buried
44 Q E -0.8696 Soluble
45 A E -1.2208 Soluble
46 P E -0.9697 Soluble
47 G E -1.4478 Soluble
48 K E -2.4337 Soluble
49 G E -1.7190 Soluble
50 L E 0.0000 Buried
51 E E -1.5141 Soluble
52 W E 0.0000 Buried
53 V E 0.0000 Buried
54 A E 0.0000 Buried
55 R E -0.5270 Soluble
56 I E 0.0000 Buried
57 Y E -0.2290 Soluble
58 P E 0.0000 Buried
59 T E -1.0017 Soluble
62 N E -0.9273 Soluble
63 G E -0.4013 Soluble
64 Y E 0.4610 Aggregation-prone
65 T E -0.4296 Soluble
66 R E -1.8960 Soluble
67 Y E -1.6558 Soluble
68 A E -1.7516 Soluble
69 D E -2.5828 Soluble
70 S E -1.6643 Soluble
71 V E 0.0000 Buried
72 K E -2.7753 Soluble
74 G E -1.7395 Soluble
75 R E -1.3243 Soluble
76 F E 0.0000 Buried
77 T E -0.9819 Soluble
78 I E 0.0000 Buried
79 S E -0.2151 Soluble
80 A E -0.8881 Soluble
81 D E -1.7417 Soluble
82 T E -1.4576 Soluble
83 S E -1.5166 Soluble
84 K E -2.2527 Soluble
85 N E -1.5889 Soluble
86 T E 0.0000 Buried
87 A E 0.0000 Buried
88 Y E -0.4459 Soluble
89 L E 0.0000 Buried
90 Q E -0.9077 Soluble
91 M E 0.0000 Buried
92 N E -1.2376 Soluble
93 S E -1.1544 Soluble
94 L E 0.0000 Buried
95 R E -1.8085 Soluble
96 A E -1.4312 Soluble
97 E E -2.0653 Soluble
98 D E 0.0000 Buried
99 T E -0.5682 Soluble
100 A E 0.0000 Buried
101 V E 0.1950 Aggregation-prone
102 Y E 0.0000 Buried
103 Y E 0.0000 Buried
104 C E 0.0000 Buried
105 S E 0.0000 Buried
106 R E -0.1218 Soluble
107 W E 0.0000 Buried
108 G E -0.6691 Soluble
109 G E -1.5439 Soluble
110 D E -2.2123 Soluble
111 G E -1.0791 Soluble
112 F E -0.2685 Soluble
113 Y E 0.4679 Aggregation-prone
114 A E 0.0000 Buried
115 M E 0.0000 Buried
116 D E -0.2589 Soluble
117 Y E 0.0017 Aggregation-prone
118 W E 0.0000 Buried
119 G E 0.0000 Buried
120 Q E -1.4544 Soluble
121 G E 0.0000 Buried
122 T E -0.0736 Soluble
123 L E 0.0562 Aggregation-prone
124 V E 0.0000 Buried
125 T E -0.3059 Soluble
126 V E 0.0000 Buried
127 S E -0.5739 Soluble
128 S E -0.7159 Soluble
129 A E -0.4193 Soluble
130 S E -0.5336 Soluble
131 T E -0.5244 Soluble
132 K E -0.9701 Soluble
133 G E -1.1141 Soluble
134 P E 0.0000 Buried
135 S E -0.1910 Soluble
136 V E 0.0000 Buried
137 F E -0.5714 Soluble
138 P E -0.8732 Soluble
139 L E 0.0000 Buried
140 A E -1.1194 Soluble
141 P E 0.0000 Buried
142 S E -1.0730 Soluble
143 S E -1.5751 Soluble
144 K E -2.1370 Soluble
145 S E -1.2082 Soluble
146 T E -1.0142 Soluble
147 S E -0.8988 Soluble
148 G E -1.0286 Soluble
149 G E -0.7221 Soluble
150 T E -0.4867 Soluble
151 A E 0.0000 Buried
152 A E 0.0000 Buried
153 L E 0.0000 Buried
154 G E 0.0000 Buried
155 C E 0.0000 Buried
156 L E 0.0000 Buried
157 V E 0.0000 Buried
158 K E -0.2536 Soluble
159 D E -0.3612 Soluble
160 Y E 0.0000 Buried
161 F E -0.1759 Soluble
162 P E 0.0000 Buried
163 E E -0.5814 Soluble
164 P E -0.6302 Soluble
165 V E -0.3644 Soluble
166 T E -0.5194 Soluble
167 V E -0.1544 Soluble
168 S E -0.4009 Soluble
169 W E 0.0000 Buried
170 N E -0.7107 Soluble
171 S E -0.6614 Soluble
172 G E -0.5513 Soluble
173 A E -0.1561 Soluble
174 L E 0.0886 Aggregation-prone
175 T E -0.1192 Soluble
176 S E -0.1254 Soluble
177 G E -0.1139 Soluble
178 V E 0.2615 Aggregation-prone
179 H E -0.2266 Soluble
180 T E 0.0553 Aggregation-prone
181 F E 0.0000 Buried
182 P E -0.3393 Soluble
183 A E 0.2473 Aggregation-prone
184 V E 0.4839 Aggregation-prone
185 L E 1.4149 Aggregation-prone
186 Q E 0.2100 Aggregation-prone
187 S E -0.0436 Soluble
188 S E -0.1851 Soluble
189 G E 0.1185 Aggregation-prone
190 L E 0.2259 Aggregation-prone
191 Y E 0.5421 Aggregation-prone
192 S E 0.1836 Aggregation-prone
193 L E 0.0000 Buried
194 S E 0.0000 Buried
195 S E 0.0000 Buried
196 V E 0.0000 Buried
197 V E 0.0000 Buried
198 T E -0.0577 Soluble
199 V E 0.0000 Buried
200 P E -0.5188 Soluble
201 S E 0.0000 Buried
202 S E -0.7357 Soluble
203 S E -0.7072 Soluble
204 L E -0.9417 Soluble
205 G E -1.0318 Soluble
206 T E -0.8409 Soluble
207 Q E -1.3390 Soluble
208 T E -1.0787 Soluble
209 Y E 0.0000 Buried
210 I E -1.0057 Soluble
211 C E 0.0000 Buried
212 N E -1.4472 Soluble
213 V E 0.0000 Buried
214 N E -1.8333 Soluble
215 H E 0.0000 Buried
216 K E -2.8042 Soluble
217 P E -1.5300 Soluble
218 S E -1.8192 Soluble
219 N E -2.5437 Soluble
220 T E -1.9825 Soluble
221 K E -2.7299 Soluble
222 V E -1.6765 Soluble
223 D E -2.5088 Soluble
224 K E -2.0959 Soluble
225 K E -2.1193 Soluble
226 V E 0.0000 Buried
227 E E -2.4040 Soluble
228 P E -1.7882 Soluble
229 K E -2.3676 Soluble
1 D F -2.3166 Soluble
2 I F 0.0000 Buried
3 Q F -2.3037 Soluble
4 M F 0.0000 Buried
5 T F -1.3983 Soluble
6 Q F 0.0000 Buried
7 S F -0.9203 Soluble
8 P F -0.7293 Soluble
9 S F -0.9849 Soluble
10 S F -0.9463 Soluble
11 L F -0.7980 Soluble
12 S F -1.0409 Soluble
13 A F -0.9610 Soluble
14 S F -0.8516 Soluble
15 V F 0.1843 Aggregation-prone
16 G F -0.3794 Soluble
17 D F -1.2103 Soluble
18 R F -2.0808 Soluble
19 V F 0.0000 Buried
20 T F -0.5862 Soluble
21 I F 0.0000 Buried
22 T F -0.8163 Soluble
23 C F 0.0000 Buried
24 R F -2.7580 Soluble
25 A F -2.4133 Soluble
26 S F -2.1739 Soluble
27 Q F -2.9483 Soluble
28 D F -2.9410 Soluble
29 V F 0.0000 Buried
36 N F -1.9445 Soluble
37 T F -0.8193 Soluble
38 A F 0.0000 Buried
39 V F 0.0000 Buried
40 A F 0.0000 Buried
41 W F 0.0000 Buried
42 Y F 0.0000 Buried
43 Q F 0.0000 Buried
44 Q F -1.3047 Soluble
45 K F -1.6110 Soluble
46 P F -1.4510 Soluble
47 G F -1.5896 Soluble
48 K F -2.5883 Soluble
49 A F -1.6560 Soluble
50 P F 0.0000 Buried
51 K F -1.4458 Soluble
52 L F 0.0000 Buried
53 L F 0.0000 Buried
54 I F 0.0000 Buried
55 Y F 1.0644 Aggregation-prone
56 S F 0.4653 Aggregation-prone
57 A F 0.0000 Buried
65 S F 0.5616 Aggregation-prone
66 F F 2.1175 Aggregation-prone
67 L F 1.3913 Aggregation-prone
68 Y F 0.7537 Aggregation-prone
69 S F 0.1225 Aggregation-prone
70 G F -0.3810 Soluble
71 V F -0.1909 Soluble
72 P F -0.2699 Soluble
74 S F -0.4044 Soluble
75 R F -0.7517 Soluble
76 F F 0.0000 Buried
77 S F 0.0172 Aggregation-prone
78 G F -0.0076 Soluble
79 S F -0.6646 Soluble
80 R F -1.7254 Soluble
83 S F -1.5084 Soluble
84 G F -1.8083 Soluble
85 T F -2.3483 Soluble
86 D F -1.9828 Soluble
87 F F 0.0000 Buried
88 T F -0.8166 Soluble
89 L F 0.0000 Buried
90 T F -0.6486 Soluble
91 I F 0.0000 Buried
92 S F -1.1358 Soluble
93 S F -0.8855 Soluble
94 L F 0.0000 Buried
95 Q F -0.6783 Soluble
96 P F -1.4128 Soluble
97 E F -1.5388 Soluble
98 D F 0.0000 Buried
99 F F -1.1197 Soluble
100 A F 0.0000 Buried
101 T F -0.9930 Soluble
102 Y F 0.0000 Buried
103 Y F 0.0000 Buried
104 C F 0.0000 Buried
105 Q F 0.0000 Buried
106 Q F 0.0000 Buried
107 H F 0.5340 Aggregation-prone
108 Y F 0.8494 Aggregation-prone
109 T F 0.2542 Aggregation-prone
114 T F -0.4233 Soluble
115 P F -0.9028 Soluble
116 P F 0.0000 Buried
117 T F -0.7461 Soluble
118 F F -0.5805 Soluble
119 G F 0.0000 Buried
120 Q F -1.9523 Soluble
121 G F 0.0000 Buried
122 T F 0.0000 Buried
123 K F -1.8115 Soluble
124 V F 0.0000 Buried
125 E F -1.4342 Soluble
126 I F 0.0000 Buried
127 K F -1.6455 Soluble
128 R F -0.9279 Soluble
129 T F -0.1169 Soluble
130 V F 0.4985 Aggregation-prone
131 A F 0.0616 Aggregation-prone
132 A F 0.0337 Aggregation-prone
133 P F 0.0000 Buried
134 S F -0.1061 Soluble
135 V F 0.0000 Buried
136 F F 0.2215 Aggregation-prone
137 I F 0.3135 Aggregation-prone
138 F F 0.0000 Buried
139 P F -0.4207 Soluble
140 P F -0.7525 Soluble
141 S F -1.5809 Soluble
142 D F -2.8316 Soluble
143 E F -2.5114 Soluble
144 Q F 0.0000 Buried
145 L F -2.2308 Soluble
146 K F -2.7733 Soluble
147 S F -1.7277 Soluble
148 G F -1.6511 Soluble
149 T F -1.0408 Soluble
150 A F 0.0000 Buried
151 S F 0.0000 Buried
152 V F 0.0000 Buried
153 V F 0.0000 Buried
154 C F 0.0000 Buried
155 L F 0.0000 Buried
156 L F 0.0000 Buried
157 N F 0.0000 Buried
158 N F -0.7349 Soluble
159 F F 0.0000 Buried
160 Y F -0.8509 Soluble
161 P F -1.4193 Soluble
162 R F -2.1175 Soluble
163 E F -2.8812 Soluble
164 A F -2.1143 Soluble
165 K F -2.3052 Soluble
166 V F -1.1191 Soluble
167 Q F -0.6481 Soluble
168 W F 0.0000 Buried
169 K F -0.5651 Soluble
170 V F 0.0000 Buried
171 D F -1.7624 Soluble
172 N F -1.3802 Soluble
173 A F -0.3730 Soluble
174 L F 0.4875 Aggregation-prone
175 Q F -0.7093 Soluble
177 G F -1.4759 Soluble
178 N F -1.9254 Soluble
179 S F -1.9668 Soluble
180 Q F -2.2181 Soluble
181 E F -2.4175 Soluble
182 S F -1.1025 Soluble
183 V F -0.8602 Soluble
184 T F -0.8741 Soluble
185 E F -1.6853 Soluble
186 Q F 0.0000 Buried
187 D F -1.8131 Soluble
188 S F -1.9435 Soluble
189 K F -2.4163 Soluble
190 D F -1.7446 Soluble
191 S F 0.0000 Buried
192 T F 0.0000 Buried
193 Y F 0.0000 Buried
194 S F 0.0000 Buried
195 L F 0.0000 Buried
196 S F 0.0000 Buried
197 S F 0.0000 Buried
198 T F -0.9915 Soluble
199 L F 0.0000 Buried
200 T F -0.7952 Soluble
201 L F -0.8970 Soluble
202 S F -1.1789 Soluble
203 K F -2.4744 Soluble
204 A F -1.9517 Soluble
205 D F -2.6531 Soluble
206 Y F 0.0000 Buried
207 E F -3.4118 Soluble
208 K F -3.5128 Soluble
209 H F -2.7446 Soluble
210 K F -2.7388 Soluble
211 V F -1.0850 Soluble
212 Y F 0.0000 Buried
213 A F -0.3666 Soluble
214 C F 0.0000 Buried
215 E F -0.5789 Soluble
216 V F 0.0000 Buried
217 T F -1.0565 Soluble
218 H F 0.0000 Buried
219 Q F -1.1483 Soluble
220 G F -0.1760 Soluble
221 L F 0.1780 Aggregation-prone
222 C F 0.4540 Aggregation-prone
223 S F 0.0148 Aggregation-prone
224 P F -0.3141 Soluble
225 V F 0.1588 Aggregation-prone
226 T F -0.2187 Soluble
227 K F -0.2107 Soluble
228 C F 0.3742 Aggregation-prone
229 F F -0.3097 Soluble
230 N F -1.6375 Soluble
231 R F -2.1195 Soluble
232 G F -1.2005 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6966 3.6935 CSV PDB
4.5 -0.7388 3.6935 CSV PDB
5.0 -0.7861 3.6935 CSV PDB
5.5 -0.8295 3.6935 CSV PDB
6.0 -0.8583 3.6935 CSV PDB
6.5 -0.8642 3.6935 CSV PDB
7.0 -0.8478 3.6935 CSV PDB
7.5 -0.8169 3.6935 CSV PDB
8.0 -0.7776 3.6935 CSV PDB
8.5 -0.7313 3.6934 CSV PDB
9.0 -0.6773 3.6931 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH