Tomuzotuximab / 5icz

A4DAB272_5icz_D_C | Source PDB: 5icz | Chains: D, C | Done
99.56
Yes
10 Å
Yes
Avg A4D Score
-0.6811
Max Score
1.9825
Min Score
-3.8162
Total Score
-290.14
Score CDR
-0.2896
Score non-CDR
-0.7285

Calculation Parameters

Chain Sequence(s) QVQLKQSGPGLVQPSQSLSITCTVSGFSLTNYGVHWVRQSPGKGLEWLGVIWSGGNTDYNTPFTSRLSINKDNSKSQVFFKMNSLQSNDTAIYYCARALTYYDYEFAYWGQGTLVTVSAASTKGPSVFPLAPGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKDILLTQSPVILSVSPGERVSFSCRASQSIGTNIHWYQQRTNGSPRLLIKYASESISGIPSRFSGSGSGTDFTLSINSVESEDIADYYCQQNNNWPTTFGAGTKLELKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGA input.pdb
Selected Chain(s) D (Heavy), C (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.8162
Maximum Score 1.9825
Average Score -0.6811
Total Score -290.138

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q D -1.3669 Soluble
2 V D -0.6981 Soluble
3 Q D -1.2496 Soluble
4 L D 0.0000 Buried
5 K D -2.1090 Soluble
6 Q D 0.0000 Buried
7 S D -0.7893 Soluble
8 G D -0.9821 Soluble
9 P D -0.4532 Soluble
11 G D -0.4185 Soluble
12 L D -0.1452 Soluble
13 V D 0.0000 Buried
14 Q D -1.7860 Soluble
15 P D -1.6365 Soluble
16 S D -1.6809 Soluble
17 Q D -2.1070 Soluble
18 S D -1.9197 Soluble
19 L D 0.0000 Buried
20 S D -0.8780 Soluble
21 I D 0.0000 Buried
22 T D -0.5042 Soluble
23 C D 0.0000 Buried
24 T D -1.0580 Soluble
25 V D 0.0000 Buried
26 S D -0.9445 Soluble
27 G D -0.8851 Soluble
28 F D -0.5037 Soluble
29 S D -0.8575 Soluble
30 L D 0.0000 Buried
35 T D -0.8740 Soluble
36 N D -0.8298 Soluble
37 Y D 0.4826 Aggregation-prone
38 G D 0.0000 Buried
39 V D 0.0000 Buried
40 H D 0.0000 Buried
41 W D 0.0000 Buried
42 V D 0.0000 Buried
43 R D 0.0000 Buried
44 Q D -0.5098 Soluble
45 S D -1.0263 Soluble
46 P D -1.1906 Soluble
47 G D -1.7508 Soluble
48 K D -2.2346 Soluble
49 G D -1.2184 Soluble
50 L D 0.0000 Buried
51 E D 0.0000 Buried
52 W D 0.0000 Buried
53 L D 0.0000 Buried
54 G D 0.0000 Buried
55 V D 0.0000 Buried
56 I D 0.0000 Buried
57 W D -0.3458 Soluble
58 S D -0.5804 Soluble
59 G D -1.1080 Soluble
63 G D -1.4653 Soluble
64 N D -1.8478 Soluble
65 T D -1.3451 Soluble
66 D D -1.1064 Soluble
67 Y D -0.4002 Soluble
68 N D 0.0000 Buried
69 T D -0.1946 Soluble
70 P D -0.3550 Soluble
71 F D 0.0000 Buried
72 T D -0.4561 Soluble
74 S D -0.6479 Soluble
75 R D -1.2588 Soluble
76 L D 0.0000 Buried
77 S D -1.2546 Soluble
78 I D 0.0000 Buried
79 N D -1.7398 Soluble
80 K D -1.3807 Soluble
81 D D -1.7574 Soluble
82 N D -2.1165 Soluble
83 S D -1.6735 Soluble
84 K D -2.3449 Soluble
85 S D -1.6177 Soluble
86 Q D -1.4719 Soluble
87 V D 0.0000 Buried
88 F D -0.2316 Soluble
89 F D 0.0000 Buried
90 K D -2.0007 Soluble
91 M D 0.0000 Buried
92 N D -2.3401 Soluble
93 S D -1.7177 Soluble
94 L D 0.0000 Buried
95 Q D -2.1062 Soluble
96 S D -1.5225 Soluble
97 N D -2.0279 Soluble
98 D D 0.0000 Buried
99 T D -0.6464 Soluble
100 A D 0.0000 Buried
101 I D 0.3846 Aggregation-prone
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 A D 0.0000 Buried
106 R D 0.0000 Buried
107 A D 0.0000 Buried
108 L D 1.0206 Aggregation-prone
109 T D 1.1493 Aggregation-prone
110 Y D 1.8494 Aggregation-prone
111 Y D 1.9494 Aggregation-prone
112 D D 0.0000 Buried
113 Y D 0.0000 Buried
114 E D 0.0000 Buried
115 F D 0.0000 Buried
116 A D 0.2084 Aggregation-prone
117 Y D 0.2410 Aggregation-prone
118 W D -0.4116 Soluble
119 G D 0.0000 Buried
120 Q D -1.7502 Soluble
121 G D -0.9125 Soluble
122 T D 0.0000 Buried
123 L D 0.1650 Aggregation-prone
124 V D 0.0000 Buried
125 T D -0.4035 Soluble
126 V D 0.0000 Buried
127 S D -0.6303 Soluble
128 A D -0.4457 Soluble
129 A D -0.3667 Soluble
130 S D -0.5791 Soluble
131 T D -0.7053 Soluble
132 K D -1.4238 Soluble
133 G D -1.5337 Soluble
134 P D -0.7276 Soluble
135 S D -0.4767 Soluble
136 V D 0.0000 Buried
137 F D 0.0000 Buried
138 P D 0.0000 Buried
139 L D 0.0000 Buried
140 A D -0.9749 Soluble
141 P D -0.6088 Soluble
142 G D -0.6334 Soluble
143 T D -0.4829 Soluble
144 A D -0.2543 Soluble
145 A D 0.0000 Buried
146 L D 0.0000 Buried
147 G D 0.0000 Buried
148 C D 0.0000 Buried
149 L D 0.0000 Buried
150 V D 0.0000 Buried
151 K D 0.0000 Buried
152 D D -0.5169 Soluble
153 Y D 0.0000 Buried
154 F D 0.0000 Buried
155 P D 0.0000 Buried
156 E D -0.3863 Soluble
157 P D -0.6210 Soluble
158 V D -0.6820 Soluble
159 T D -0.6465 Soluble
160 V D -0.2640 Soluble
161 S D -0.4574 Soluble
162 W D 0.0000 Buried
163 N D -0.8130 Soluble
164 S D -0.6965 Soluble
165 G D -0.5411 Soluble
166 A D -0.2507 Soluble
167 L D -0.0116 Soluble
168 T D -0.1678 Soluble
169 S D -0.1872 Soluble
170 G D -0.2206 Soluble
171 V D 0.1537 Aggregation-prone
172 H D -0.2816 Soluble
173 T D 0.0120 Aggregation-prone
174 F D 0.0000 Buried
175 P D -0.4547 Soluble
176 A D 0.2778 Aggregation-prone
177 V D 0.6033 Aggregation-prone
178 L D 1.3127 Aggregation-prone
179 Q D 0.3283 Aggregation-prone
180 S D -0.0362 Soluble
181 S D -0.2144 Soluble
182 G D 0.0332 Aggregation-prone
183 L D 0.0796 Aggregation-prone
184 Y D 0.4548 Aggregation-prone
185 S D 0.0000 Buried
186 L D 0.3675 Aggregation-prone
187 S D 0.0000 Buried
188 S D 0.0000 Buried
189 V D 0.0000 Buried
190 V D 0.0000 Buried
191 T D -0.1391 Soluble
192 V D 0.0000 Buried
193 P D -0.5621 Soluble
194 S D -0.5072 Soluble
195 S D -0.5947 Soluble
196 S D -0.5849 Soluble
197 L D -0.6813 Soluble
198 G D -0.8745 Soluble
199 T D -0.6958 Soluble
200 Q D -1.1499 Soluble
201 T D -1.0111 Soluble
202 Y D 0.0000 Buried
203 I D -1.1584 Soluble
204 C D 0.0000 Buried
205 N D -1.4978 Soluble
206 V D 0.0000 Buried
207 N D -2.1606 Soluble
208 H D 0.0000 Buried
209 K D -2.6547 Soluble
210 P D -1.5624 Soluble
211 S D -1.8284 Soluble
212 N D -2.5448 Soluble
213 T D -2.1103 Soluble
214 K D -2.7237 Soluble
215 V D -1.6437 Soluble
216 D D -2.4673 Soluble
217 K D -1.9832 Soluble
218 R D -2.3012 Soluble
219 V D 0.0000 Buried
220 E D -2.0910 Soluble
221 P D -1.5066 Soluble
222 K D -2.0407 Soluble
1 D C -1.2398 Soluble
2 I C -0.2790 Soluble
3 L C 0.7192 Aggregation-prone
4 L C 0.0000 Buried
5 T C -0.3688 Soluble
6 Q C 0.0000 Buried
7 S C 0.1941 Aggregation-prone
8 P C 0.9231 Aggregation-prone
9 V C 1.9825 Aggregation-prone
10 I C 1.5306 Aggregation-prone
11 L C 0.6058 Aggregation-prone
12 S C -0.2072 Soluble
13 V C 0.0000 Buried
14 S C -1.8008 Soluble
15 P C -1.9162 Soluble
16 G C -2.2461 Soluble
17 E C -3.1705 Soluble
18 R C -3.4951 Soluble
19 V C 0.0000 Buried
20 S C -0.9342 Soluble
21 F C 0.0000 Buried
22 S C -0.8113 Soluble
23 C C 0.0000 Buried
24 R C -2.4817 Soluble
25 A C 0.0000 Buried
26 S C -0.9362 Soluble
27 Q C -1.2809 Soluble
28 S C -1.4365 Soluble
29 I C 0.0000 Buried
36 G C -0.9270 Soluble
37 T C -0.7369 Soluble
38 N C -0.7835 Soluble
39 I C 0.0000 Buried
40 H C 0.0000 Buried
41 W C 0.0000 Buried
42 Y C 0.0000 Buried
43 Q C 0.0000 Buried
44 Q C -1.3219 Soluble
45 R C -2.0330 Soluble
46 T C -1.7382 Soluble
47 N C -1.8399 Soluble
48 G C -1.5348 Soluble
49 S C -1.0222 Soluble
50 P C 0.0000 Buried
51 R C -0.7269 Soluble
52 L C 0.0000 Buried
53 L C 0.0000 Buried
54 I C 0.0000 Buried
55 K C -0.8466 Soluble
56 Y C -0.5913 Soluble
57 A C 0.0000 Buried
65 S C -1.2158 Soluble
66 E C -1.8913 Soluble
67 S C -1.1476 Soluble
68 I C -0.4996 Soluble
69 S C -0.3389 Soluble
70 G C -0.4764 Soluble
71 I C -0.2903 Soluble
72 P C -0.3823 Soluble
74 S C -0.6908 Soluble
75 R C -1.3295 Soluble
76 F C 0.0000 Buried
77 S C -0.9715 Soluble
78 G C -0.7160 Soluble
79 S C -0.8487 Soluble
80 G C -1.0899 Soluble
83 S C -0.9322 Soluble
84 G C -1.2950 Soluble
85 T C -1.8998 Soluble
86 D C -2.5765 Soluble
87 F C 0.0000 Buried
88 T C -0.8905 Soluble
89 L C 0.0000 Buried
90 S C 0.0000 Buried
91 I C 0.0000 Buried
92 N C -2.8735 Soluble
93 S C -2.4832 Soluble
94 V C 0.0000 Buried
95 E C -2.5997 Soluble
96 S C -2.3768 Soluble
97 E C -2.4732 Soluble
98 D C 0.0000 Buried
99 I C -1.6073 Soluble
100 A C 0.0000 Buried
101 D C -0.9119 Soluble
102 Y C 0.0000 Buried
103 Y C 0.0000 Buried
104 C C 0.0000 Buried
105 Q C 0.0000 Buried
106 Q C 0.0000 Buried
107 N C -0.6568 Soluble
108 N C -1.1517 Soluble
109 N C -0.9719 Soluble
114 W C 0.1484 Aggregation-prone
115 P C 0.0000 Buried
116 T C 0.0000 Buried
117 T C 0.0252 Aggregation-prone
118 F C 0.2307 Aggregation-prone
119 G C 0.0000 Buried
120 A C 0.0501 Aggregation-prone
121 G C 0.1183 Aggregation-prone
122 T C 0.0000 Buried
123 K C -0.2069 Soluble
124 L C 0.0000 Buried
125 E C -0.9557 Soluble
126 L C 0.0000 Buried
127 K C -1.5368 Soluble
128 R C -1.1114 Soluble
129 T C -0.0161 Soluble
130 V C 0.5641 Aggregation-prone
131 A C 0.2690 Aggregation-prone
132 A C -0.0194 Soluble
133 P C 0.0000 Buried
134 S C -0.1458 Soluble
135 V C 0.0000 Buried
136 F C 0.1006 Aggregation-prone
137 I C 0.0896 Aggregation-prone
138 F C 0.0000 Buried
139 P C -0.5422 Soluble
140 P C -0.7713 Soluble
141 S C -1.7707 Soluble
142 D C -2.8182 Soluble
143 E C -2.7547 Soluble
144 Q C 0.0000 Buried
145 L C -2.1926 Soluble
146 K C -2.6588 Soluble
147 S C -1.6105 Soluble
148 G C -1.2510 Soluble
149 T C -0.8110 Soluble
150 A C 0.0000 Buried
151 S C 0.0000 Buried
152 V C 0.0000 Buried
153 V C 0.0000 Buried
154 C C 0.0000 Buried
155 L C 0.0000 Buried
156 L C 0.0000 Buried
157 N C 0.0000 Buried
158 N C -0.7879 Soluble
159 F C 0.0000 Buried
160 Y C 0.0000 Buried
161 P C -1.5823 Soluble
162 R C -2.7443 Soluble
163 E C -3.1922 Soluble
164 A C -2.3077 Soluble
165 K C -2.3053 Soluble
166 V C -1.1885 Soluble
167 Q C -0.8204 Soluble
168 W C 0.0000 Buried
169 K C -0.7256 Soluble
170 V C 0.0000 Buried
171 D C -1.9592 Soluble
172 N C -1.6002 Soluble
173 A C -0.3411 Soluble
174 L C 0.5256 Aggregation-prone
175 Q C -0.2170 Soluble
176 S C -0.4513 Soluble
177 G C -0.8972 Soluble
178 N C -0.8178 Soluble
179 S C -1.2040 Soluble
180 Q C -1.4821 Soluble
181 E C -2.0290 Soluble
182 S C -0.8954 Soluble
183 V C -0.8412 Soluble
184 T C -1.1084 Soluble
185 E C -2.2018 Soluble
186 Q C 0.0000 Buried
187 D C -2.0675 Soluble
188 S C -2.4897 Soluble
189 K C -2.8043 Soluble
190 D C -2.1130 Soluble
191 S C 0.0000 Buried
192 T C 0.0000 Buried
193 Y C -1.3071 Soluble
194 S C 0.0000 Buried
195 L C 0.0000 Buried
196 S C 0.0000 Buried
197 S C 0.0000 Buried
198 T C -0.6969 Soluble
199 L C 0.0000 Buried
200 T C -0.3206 Soluble
201 L C -0.7314 Soluble
202 S C -1.0989 Soluble
203 K C -2.0887 Soluble
204 A C -2.0774 Soluble
205 D C -2.8274 Soluble
206 Y C 0.0000 Buried
207 E C -3.7811 Soluble
208 K C -3.8162 Soluble
209 H C -3.2603 Soluble
210 K C -3.1234 Soluble
211 V C -1.4006 Soluble
212 Y C 0.0000 Buried
213 A C 0.0000 Buried
214 C C 0.0000 Buried
215 E C -1.0272 Soluble
216 V C 0.0000 Buried
217 T C -1.2219 Soluble
218 H C 0.0000 Buried
219 Q C -1.6959 Soluble
220 G C -0.3259 Soluble
221 L C -0.1647 Soluble
222 S C -0.4300 Soluble
223 S C -0.4252 Soluble
224 P C -0.5739 Soluble
225 V C -0.1191 Soluble
226 T C -0.5362 Soluble
227 K C -0.7647 Soluble
228 S C -0.6412 Soluble
229 F C -0.7253 Soluble
230 N C -1.6294 Soluble
231 R C -2.1877 Soluble
232 G C -1.4044 Soluble
233 A C -0.7281 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6656 2.6675 CSV PDB
4.5 -0.7036 2.5966 CSV PDB
5.0 -0.7487 2.5175 CSV PDB
5.5 -0.7923 2.4355 CSV PDB
6.0 -0.8241 2.3525 CSV PDB
6.5 -0.8356 2.2694 CSV PDB
7.0 -0.8265 2.1867 CSV PDB
7.5 -0.8031 2.173 CSV PDB
8.0 -0.7719 2.1725 CSV PDB
8.5 -0.7348 2.1709 CSV PDB
9.0 -0.6922 2.2066 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH