Solnerstotug / 8tbq

A4DAB243_8tbq_H_L | Source PDB: 8tbq | Chains: H, L | Done
99.56
Yes
10 Å
Yes
Avg A4D Score
-0.7327
Max Score
2.6561
Min Score
-3.9921
Total Score
-320.18
Score CDR
-0.4241
Score non-CDR
-0.7726

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFTFDDYSMNWVRQAPGKGLEWVSYISSDADTIYYADSVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCAREAGGVLGRVDFQHWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKDIQLTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIYDASNLGTGVPSRFSGSGSGTDFTFTISSLQPEDIATYYCQQADFVPPWTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.9921
Maximum Score 2.6561
Average Score -0.7327
Total Score -320.1825

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.1870 Soluble
2 V H -1.5261 Soluble
3 Q H -1.4476 Soluble
4 L H 0.0000 Buried
5 V H 1.1608 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.2990 Soluble
8 G H -0.9324 Soluble
9 G H -0.5292 Soluble
11 G H -0.4923 Soluble
12 L H -0.1194 Soluble
13 V H 0.0000 Buried
14 Q H -1.6922 Soluble
15 P H -1.5871 Soluble
16 G H -1.4512 Soluble
17 G H -1.1245 Soluble
18 S H -1.0368 Soluble
19 L H -1.0099 Soluble
20 R H -1.8369 Soluble
21 L H 0.0000 Buried
22 S H -0.3065 Soluble
23 C H 0.0000 Buried
24 A H -0.0038 Soluble
25 A H 0.0000 Buried
26 S H -1.0739 Soluble
27 G H -1.3768 Soluble
28 F H -1.0302 Soluble
29 T H -1.1653 Soluble
30 F H 0.0000 Buried
35 D H -3.1696 Soluble
36 D H -2.8731 Soluble
37 Y H -1.4409 Soluble
38 S H -0.6375 Soluble
39 M H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.9100 Soluble
45 A H -1.2427 Soluble
46 P H -0.9688 Soluble
47 G H -1.4730 Soluble
48 K H -2.3406 Soluble
49 G H -1.6082 Soluble
50 L H 0.0000 Buried
51 E H -1.4526 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 Y H 0.5796 Aggregation-prone
56 I H 0.0000 Buried
57 S H -1.2170 Soluble
58 S H -2.1841 Soluble
59 D H -3.0968 Soluble
62 A H -1.8189 Soluble
63 D H -2.1417 Soluble
64 T H -0.6611 Soluble
65 I H 0.6631 Aggregation-prone
66 Y H 0.7042 Aggregation-prone
67 Y H -0.4664 Soluble
68 A H -1.3779 Soluble
69 D H -2.4845 Soluble
70 S H -1.7921 Soluble
71 V H 0.0000 Buried
72 K H -2.5925 Soluble
74 G H -1.7892 Soluble
75 R H -1.5149 Soluble
76 F H 0.0000 Buried
77 T H -0.6330 Soluble
78 I H 0.0000 Buried
79 S H -0.3663 Soluble
80 R H -1.2696 Soluble
81 D H -1.7649 Soluble
82 N H -2.3162 Soluble
83 A H -1.4854 Soluble
84 K H -2.3881 Soluble
85 N H -1.9779 Soluble
86 S H -1.1015 Soluble
87 L H 0.0000 Buried
88 Y H -0.4546 Soluble
89 L H 0.0000 Buried
90 Q H -0.9911 Soluble
91 M H 0.0000 Buried
92 N H -1.3308 Soluble
93 S H -1.2870 Soluble
94 L H 0.0000 Buried
95 R H -2.4695 Soluble
96 A H -1.8055 Soluble
97 E H -2.2824 Soluble
98 D H 0.0000 Buried
99 T H -0.7656 Soluble
100 A H 0.0000 Buried
101 V H 0.2140 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 E H 0.0000 Buried
108 A H 0.0000 Buried
109 G H -0.0770 Soluble
110 G H 0.4778 Aggregation-prone
111 V H 2.0578 Aggregation-prone
111A L H 1.9694 Aggregation-prone
112A G H 0.6700 Aggregation-prone
112 R H 0.2748 Aggregation-prone
113 V H 0.5911 Aggregation-prone
114 D H 0.0000 Buried
115 F H 0.0000 Buried
116 Q H -0.5784 Soluble
117 H H -0.6766 Soluble
118 W H -0.4968 Soluble
119 G H 0.0000 Buried
120 Q H -1.2762 Soluble
121 G H 0.0000 Buried
122 T H 0.0399 Aggregation-prone
123 L H 0.0496 Aggregation-prone
124 V H 0.0000 Buried
125 T H -0.5058 Soluble
126 V H 0.0000 Buried
127 S H -0.8053 Soluble
128 S H -0.6944 Soluble
129 A H -0.4917 Soluble
130 S H -0.6304 Soluble
131 T H -0.7478 Soluble
132 K H -1.5163 Soluble
133 G H -1.6037 Soluble
134 P H -0.8059 Soluble
135 S H -0.5542 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.2148 Soluble
139 L H 0.0000 Buried
140 A H -1.2961 Soluble
141 P H 0.0000 Buried
142 S H -1.6009 Soluble
143 S H -1.3567 Soluble
144 K H -1.8636 Soluble
145 S H 0.0000 Buried
146 T H -0.9439 Soluble
147 S H -0.8360 Soluble
148 G H -0.8021 Soluble
149 G H -0.8854 Soluble
150 T H -0.6244 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H 0.0000 Buried
159 D H -0.8416 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H 0.0000 Buried
163 E H -0.6331 Soluble
164 P H -0.7069 Soluble
165 V H -0.6960 Soluble
166 T H -0.6067 Soluble
167 V H -0.4300 Soluble
168 S H -0.4086 Soluble
169 W H 0.0000 Buried
170 N H -0.7506 Soluble
171 S H -0.6404 Soluble
172 G H -0.4660 Soluble
173 A H -0.2175 Soluble
174 L H 0.0027 Aggregation-prone
175 T H -0.1736 Soluble
176 S H -0.1815 Soluble
177 G H -0.1478 Soluble
178 V H 0.1475 Aggregation-prone
179 H H -0.3852 Soluble
180 T H 0.0377 Aggregation-prone
181 F H 0.0000 Buried
182 P H -0.1930 Soluble
183 A H 0.4637 Aggregation-prone
184 V H 0.4263 Aggregation-prone
185 L H 1.2620 Aggregation-prone
186 Q H 0.1362 Aggregation-prone
187 S H -0.1365 Soluble
188 S H -0.3097 Soluble
189 G H -0.0849 Soluble
190 L H -0.0665 Soluble
191 Y H 0.3814 Aggregation-prone
192 S H 0.0000 Buried
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.1174 Soluble
199 V H 0.0000 Buried
200 P H -0.5180 Soluble
201 S H -0.5435 Soluble
202 S H -0.5407 Soluble
203 S H -0.5443 Soluble
204 L H -0.6984 Soluble
205 G H -0.9639 Soluble
206 T H -0.6446 Soluble
207 Q H -1.0624 Soluble
208 T H -1.0928 Soluble
209 Y H 0.0000 Buried
210 I H -1.3173 Soluble
211 C H 0.0000 Buried
212 N H -1.5258 Soluble
213 V H 0.0000 Buried
214 N H -2.1046 Soluble
215 H H 0.0000 Buried
216 K H -2.8229 Soluble
217 P H -1.6651 Soluble
218 S H -1.9050 Soluble
219 N H -2.6761 Soluble
220 T H -2.3028 Soluble
221 K H -2.7598 Soluble
222 V H -1.6385 Soluble
223 D H -2.5854 Soluble
224 K H -2.1282 Soluble
225 K H -2.6280 Soluble
226 V H 0.0000 Buried
227 E H -2.8105 Soluble
228 P H -1.8854 Soluble
229 K H -2.7379 Soluble
1 D L -2.0860 Soluble
2 I L 0.0000 Buried
3 Q L -2.1462 Soluble
4 L L 0.0000 Buried
5 T L -1.0910 Soluble
6 Q L 0.0000 Buried
7 S L -0.5250 Soluble
8 P L -0.6253 Soluble
9 S L -0.8762 Soluble
10 S L -0.9811 Soluble
11 L L -0.7131 Soluble
12 S L -0.9938 Soluble
13 A L -0.9739 Soluble
14 S L -0.8367 Soluble
15 V L 0.2368 Aggregation-prone
16 G L -0.6461 Soluble
17 D L -1.8745 Soluble
18 R L -2.3598 Soluble
19 V L 0.0000 Buried
20 T L -0.6305 Soluble
21 I L 0.0000 Buried
22 T L -0.7397 Soluble
23 C L 0.0000 Buried
24 Q L -2.1922 Soluble
25 A L 0.0000 Buried
26 S L -2.2324 Soluble
27 Q L -2.9452 Soluble
28 D L -2.9198 Soluble
29 I L 0.0000 Buried
36 S L -1.3269 Soluble
37 N L -1.0680 Soluble
38 Y L -0.0459 Soluble
39 L L 0.0000 Buried
40 N L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -1.3718 Soluble
45 K L -2.0245 Soluble
46 P L -1.6507 Soluble
47 G L -1.8694 Soluble
48 K L -2.5847 Soluble
49 A L -1.6895 Soluble
50 P L 0.0000 Buried
51 K L -1.5302 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.1352 Soluble
56 D L -0.4942 Soluble
57 A L 0.0000 Buried
65 S L -0.6260 Soluble
66 N L -0.5298 Soluble
67 L L 0.0657 Aggregation-prone
68 G L -0.2406 Soluble
69 T L -0.2975 Soluble
70 G L -0.4868 Soluble
71 V L 0.0000 Buried
72 P L -0.4178 Soluble
74 S L -0.4745 Soluble
75 R L -0.9015 Soluble
76 F L 0.0000 Buried
77 S L -0.4283 Soluble
78 G L -0.3981 Soluble
79 S L -0.9003 Soluble
80 G L -1.1701 Soluble
83 S L -1.3719 Soluble
84 G L -1.9384 Soluble
85 T L -2.3224 Soluble
86 D L -2.7080 Soluble
87 F L 0.0000 Buried
88 T L -0.7432 Soluble
89 F L 0.0000 Buried
90 T L -0.6349 Soluble
91 I L 0.0000 Buried
92 S L -1.4269 Soluble
93 S L -1.1349 Soluble
94 L L 0.0000 Buried
95 Q L -0.8263 Soluble
96 P L -1.6039 Soluble
97 E L -2.1469 Soluble
98 D L 0.0000 Buried
99 I L 0.0000 Buried
100 A L 0.0000 Buried
101 T L -1.0224 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 A L 0.0000 Buried
108 D L 0.9873 Aggregation-prone
109 F L 2.6561 Aggregation-prone
113 V L 2.2379 Aggregation-prone
114 P L 1.1060 Aggregation-prone
115 P L -0.3608 Soluble
116 W L 0.0000 Buried
117 T L -0.9631 Soluble
118 F L 0.0000 Buried
119 G L -0.8466 Soluble
120 G L -1.3438 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -1.8615 Soluble
124 V L 0.0000 Buried
125 E L -1.3477 Soluble
126 I L 0.0000 Buried
127 K L -1.2849 Soluble
128 R L -0.8661 Soluble
129 T L 0.0634 Aggregation-prone
130 V L 0.8769 Aggregation-prone
131 A L 0.3407 Aggregation-prone
132 A L 0.0288 Aggregation-prone
133 P L 0.0000 Buried
134 S L -0.1956 Soluble
135 V L 0.0000 Buried
136 F L 0.0000 Buried
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.6650 Soluble
140 P L -0.6608 Soluble
141 S L -1.6662 Soluble
142 D L -2.9213 Soluble
143 E L -2.5329 Soluble
144 Q L 0.0000 Buried
145 L L -1.9309 Soluble
146 K L -2.6884 Soluble
147 S L -1.7064 Soluble
148 G L -1.4148 Soluble
149 T L -1.1730 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.8306 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.1973 Soluble
162 R L -1.7513 Soluble
163 E L -2.6644 Soluble
164 A L -2.0470 Soluble
165 K L -2.1406 Soluble
166 V L -1.1950 Soluble
167 Q L -0.5674 Soluble
168 W L 0.0000 Buried
169 K L -0.5796 Soluble
170 V L 0.0000 Buried
171 D L -2.1042 Soluble
172 N L -1.5158 Soluble
173 A L -0.3940 Soluble
174 L L 0.7644 Aggregation-prone
175 Q L -0.3099 Soluble
176 S L -0.6658 Soluble
177 G L -1.4239 Soluble
178 N L -1.8234 Soluble
179 S L -1.7760 Soluble
180 Q L -2.0388 Soluble
181 E L -2.0249 Soluble
182 S L -0.7595 Soluble
183 V L -0.5529 Soluble
184 T L -0.9397 Soluble
185 E L -2.1704 Soluble
186 Q L 0.0000 Buried
187 D L -2.2199 Soluble
188 S L -2.3564 Soluble
189 K L -2.6948 Soluble
190 D L -2.0641 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.9276 Soluble
199 L L 0.0000 Buried
200 T L -0.5191 Soluble
201 L L -0.6768 Soluble
202 S L -1.1246 Soluble
203 K L -2.0796 Soluble
204 A L -2.0275 Soluble
205 D L -3.1899 Soluble
206 Y L 0.0000 Buried
207 E L -3.7970 Soluble
208 K L -3.9921 Soluble
209 H L -3.5195 Soluble
210 K L -3.1077 Soluble
211 V L -1.5964 Soluble
212 Y L 0.0000 Buried
213 A L -0.7855 Soluble
214 C L 0.0000 Buried
215 E L -0.9923 Soluble
216 V L 0.0000 Buried
217 T L -1.2115 Soluble
218 H L 0.0000 Buried
219 Q L -1.2370 Soluble
220 G L -0.2859 Soluble
221 L L -0.2122 Soluble
222 S L -0.5450 Soluble
223 S L -0.4019 Soluble
224 P L -0.5769 Soluble
225 V L 0.0863 Aggregation-prone
226 T L -0.3392 Soluble
227 K L -0.6363 Soluble
228 S L -0.7498 Soluble
229 F L -1.2238 Soluble
230 N L -1.9319 Soluble
231 R L -2.1882 Soluble
232 G L -1.9249 Soluble
233 E L -2.6226 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Downloads
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6579 5.1138 CSV PDB
4.5 -0.7144 5.0775 CSV PDB
5.0 -0.7795 5.0393 CSV PDB
5.5 -0.8422 5.0005 CSV PDB
6.0 -0.8906 4.9616 CSV PDB
6.5 -0.9161 4.9228 CSV PDB
7.0 -0.9191 4.8845 CSV PDB
7.5 -0.9074 4.8477 CSV PDB
8.0 -0.8864 4.815 CSV PDB
8.5 -0.8567 4.7907 CSV PDB
9.0 -0.8169 4.7769 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH