Metelimumab / 6amj

A4DAB169_6amj_H_L | Source PDB: 6amj | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6674
Max Score
1.4911
Min Score
-3.3683
Total Score
-277.65
Score CDR
-0.4651
Score non-CDR
-0.7225

Calculation Parameters

Chain Sequence(s) EVQLVESGGGVVQPGRSLRLSCAASGFTFSSYGMHWVRQAPGKELEWVAVISYDGSIKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARTGEPQYSWGQGTTVTVSSASTKGPSVFPLAPCESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSKTYTCNVDHKPSNTKVDKRVHEIVLTQSPSSLSASVGDRVTITCRASQGIGDDLGWYQQKPGKAPILLIYGTSTLQSGVPSRFSGSGSGTDFTLTINSLQPEDFATYYCLQDSNYPLTFGGGTRLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNR input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.3683
Maximum Score 1.4911
Average Score -0.6674
Total Score -277.6466

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -1.9616 Soluble
2 V H -0.9981 Soluble
3 Q H -1.2893 Soluble
4 L H 0.0000 Buried
5 V H 0.1800 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.5966 Soluble
8 G H -0.8439 Soluble
9 G H -0.4853 Soluble
11 G H 0.0716 Aggregation-prone
12 V H 0.5860 Aggregation-prone
13 V H 0.0000 Buried
14 Q H -1.6437 Soluble
15 P H -1.9362 Soluble
16 G H -2.0092 Soluble
17 R H -2.4838 Soluble
18 S H -1.7970 Soluble
19 L H -1.4034 Soluble
20 R H -2.0268 Soluble
21 L H 0.0000 Buried
22 S H -0.5452 Soluble
23 C H 0.0000 Buried
24 A H -0.3419 Soluble
25 A H 0.0000 Buried
26 S H -0.7942 Soluble
27 G H -1.0921 Soluble
28 F H -0.4027 Soluble
29 T H -0.1467 Soluble
30 F H 0.0000 Buried
35 S H -0.6885 Soluble
36 S H -0.0106 Soluble
37 Y H 0.1154 Aggregation-prone
38 G H 0.1801 Aggregation-prone
39 M H 0.0000 Buried
40 H H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.9928 Soluble
45 A H -1.3577 Soluble
46 P H -1.0909 Soluble
47 G H -1.6007 Soluble
48 K H -2.5894 Soluble
49 E H -2.0587 Soluble
50 L H 0.0000 Buried
51 E H -1.1518 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 A H 0.0000 Buried
55 V H 0.0000 Buried
56 I H 0.0000 Buried
57 S H 0.3971 Aggregation-prone
58 Y H 0.5099 Aggregation-prone
59 D H -1.1887 Soluble
62 G H -0.5577 Soluble
63 S H 0.0059 Aggregation-prone
64 I H 1.2053 Aggregation-prone
65 K H 0.4486 Aggregation-prone
66 Y H 0.6673 Aggregation-prone
67 Y H -0.5364 Soluble
68 A H 0.0000 Buried
69 D H -2.4856 Soluble
70 S H -1.7224 Soluble
71 V H 0.0000 Buried
72 K H -2.4745 Soluble
74 G H -1.7133 Soluble
75 R H -1.3979 Soluble
76 F H 0.0000 Buried
77 T H -0.8337 Soluble
78 I H 0.0000 Buried
79 S H -0.5248 Soluble
80 R H -1.0651 Soluble
81 D H -1.8057 Soluble
82 N H -1.7615 Soluble
83 S H -1.6164 Soluble
84 K H -2.4077 Soluble
85 N H -1.7676 Soluble
86 T H -1.1065 Soluble
87 L H 0.0000 Buried
88 Y H -0.5506 Soluble
89 L H 0.0000 Buried
90 Q H -1.3096 Soluble
91 M H 0.0000 Buried
92 N H -1.6639 Soluble
93 S H -1.6044 Soluble
94 L H 0.0000 Buried
95 R H -2.4977 Soluble
96 A H -1.7169 Soluble
97 E H -2.2618 Soluble
98 D H 0.0000 Buried
99 T H -0.7182 Soluble
100 A H 0.0000 Buried
101 V H 0.0650 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 T H -0.3470 Soluble
108 G H -1.0557 Soluble
109 E H -1.8852 Soluble
114 P H -0.5082 Soluble
115 Q H -0.7055 Soluble
116 Y H 0.0000 Buried
117 S H 0.0924 Aggregation-prone
118 W H 0.0989 Aggregation-prone
119 G H 0.0000 Buried
120 Q H -1.2769 Soluble
121 G H 0.0000 Buried
122 T H -0.2978 Soluble
123 T H -0.0920 Soluble
124 V H 0.0000 Buried
125 T H -0.2942 Soluble
126 V H 0.0000 Buried
127 S H -0.5021 Soluble
128 S H -0.4587 Soluble
129 A H -0.3665 Soluble
130 S H -0.4796 Soluble
131 T H -0.5854 Soluble
132 K H -1.1441 Soluble
133 G H -1.3481 Soluble
134 P H -0.6448 Soluble
135 S H -0.4549 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.0967 Soluble
139 L H 0.0000 Buried
140 A H -0.0757 Soluble
141 P H 0.1136 Aggregation-prone
142 C H 0.1386 Aggregation-prone
143 E H -1.9991 Soluble
144 S H -1.2560 Soluble
145 T H -0.7977 Soluble
146 A H -0.5285 Soluble
147 A H 0.0000 Buried
148 L H 0.0338 Aggregation-prone
149 G H 0.0000 Buried
150 C H 0.0000 Buried
151 L H 0.0000 Buried
152 V H 0.0000 Buried
153 K H 0.0000 Buried
154 D H -0.4601 Soluble
155 Y H 0.0000 Buried
156 F H 0.0000 Buried
157 P H -0.6623 Soluble
158 E H -1.0249 Soluble
159 P H -1.2173 Soluble
160 V H -0.9846 Soluble
161 T H -0.7405 Soluble
162 V H -0.4542 Soluble
163 S H -0.5177 Soluble
164 W H 0.0000 Buried
165 N H -1.0414 Soluble
166 S H -0.7824 Soluble
167 G H -0.5713 Soluble
168 A H -0.2835 Soluble
169 L H -0.1368 Soluble
170 T H -0.2254 Soluble
171 S H -0.2289 Soluble
172 G H -0.2677 Soluble
173 V H 0.1188 Aggregation-prone
174 H H -0.2524 Soluble
175 T H 0.0639 Aggregation-prone
176 F H 0.0000 Buried
177 P H -0.1729 Soluble
178 A H 0.2779 Aggregation-prone
179 V H 0.8596 Aggregation-prone
180 L H 1.4911 Aggregation-prone
181 Q H 0.5084 Aggregation-prone
182 S H 0.0275 Aggregation-prone
183 S H -0.1626 Soluble
184 G H 0.0923 Aggregation-prone
185 L H 0.0455 Aggregation-prone
186 Y H 0.3877 Aggregation-prone
187 S H 0.0000 Buried
188 L H 0.0000 Buried
189 S H 0.0000 Buried
190 S H 0.0000 Buried
191 V H 0.0000 Buried
192 V H 0.0000 Buried
193 T H -0.1219 Soluble
194 V H 0.0000 Buried
195 P H -0.6451 Soluble
196 S H -0.9413 Soluble
197 K H -1.8658 Soluble
198 T H -1.4532 Soluble
199 Y H 0.0000 Buried
200 T H 0.0000 Buried
201 C H 0.0000 Buried
202 N H 0.0000 Buried
203 V H 0.0000 Buried
204 D H -2.1276 Soluble
205 H H 0.0000 Buried
206 K H -2.8382 Soluble
207 P H -1.7334 Soluble
208 S H -1.8107 Soluble
209 N H -2.5597 Soluble
210 T H -2.1135 Soluble
211 K H -2.7215 Soluble
212 V H -1.5655 Soluble
213 D H -2.7283 Soluble
214 K H -2.2196 Soluble
215 R H -2.7489 Soluble
216 V H 0.0000 Buried
217 H H -1.6546 Soluble
1 E L -1.7765 Soluble
2 I L 0.0000 Buried
3 V L 0.3457 Aggregation-prone
4 L L 0.0000 Buried
5 T L -0.6952 Soluble
6 Q L 0.0000 Buried
7 S L -0.6206 Soluble
8 P L -0.4291 Soluble
9 S L -0.5725 Soluble
10 S L -0.4090 Soluble
11 L L -0.1999 Soluble
12 S L -0.6550 Soluble
13 A L 0.0000 Buried
14 S L -0.3181 Soluble
15 V L 0.3892 Aggregation-prone
16 G L -0.8151 Soluble
17 D L -1.6113 Soluble
18 R L -2.6145 Soluble
19 V L 0.0000 Buried
20 T L -0.6312 Soluble
21 I L 0.0000 Buried
22 T L -0.7873 Soluble
23 C L 0.0000 Buried
24 R L -2.4198 Soluble
25 A L 0.0000 Buried
26 S L -1.2137 Soluble
27 Q L -1.9394 Soluble
28 G L -1.9341 Soluble
29 I L 0.0000 Buried
36 G L -1.9176 Soluble
37 D L -2.4742 Soluble
38 D L -2.0910 Soluble
39 L L 0.0000 Buried
40 G L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.0592 Soluble
46 P L -1.1533 Soluble
47 G L -1.1771 Soluble
48 K L -1.1684 Soluble
49 A L -0.5636 Soluble
50 P L 0.0000 Buried
51 I L 1.0670 Aggregation-prone
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.3720 Soluble
56 G L -1.0183 Soluble
57 T L 0.0000 Buried
65 S L -0.6624 Soluble
66 T L -0.2096 Soluble
67 L L 0.0935 Aggregation-prone
68 Q L -0.1097 Soluble
69 S L -0.4202 Soluble
70 G L -0.5301 Soluble
71 V L -0.0813 Soluble
72 P L -0.4750 Soluble
74 S L -0.6026 Soluble
75 R L -1.2442 Soluble
76 F L 0.0000 Buried
77 S L -0.4921 Soluble
78 G L -0.3348 Soluble
79 S L -0.8837 Soluble
80 G L -1.3378 Soluble
83 S L -1.7414 Soluble
84 G L -1.8008 Soluble
85 T L -2.0732 Soluble
86 D L -2.2205 Soluble
87 F L 0.0000 Buried
88 T L -0.7582 Soluble
89 L L 0.0000 Buried
90 T L -0.9090 Soluble
91 I L 0.0000 Buried
92 N L -2.3990 Soluble
93 S L -1.6020 Soluble
94 L L 0.0000 Buried
95 Q L -1.0285 Soluble
96 P L -0.8514 Soluble
97 E L -1.8940 Soluble
98 D L 0.0000 Buried
99 F L -0.3213 Soluble
100 A L 0.0000 Buried
101 T L -0.5027 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 L L 0.0000 Buried
106 Q L 0.0000 Buried
107 D L -0.9476 Soluble
108 S L -1.2567 Soluble
109 N L -1.2797 Soluble
114 Y L -0.0580 Soluble
115 P L -0.4527 Soluble
116 L L 0.0000 Buried
117 T L -0.3565 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 G L -0.9756 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 R L -0.7144 Soluble
124 L L 0.0000 Buried
125 E L 0.0000 Buried
126 I L -0.2145 Soluble
127 K L -1.2338 Soluble
128 R L -1.2613 Soluble
129 T L -0.3143 Soluble
130 V L 0.3887 Aggregation-prone
131 A L 0.0390 Aggregation-prone
132 A L -0.0751 Soluble
133 P L 0.0000 Buried
134 S L -0.1246 Soluble
135 V L 0.0000 Buried
136 F L 0.0000 Buried
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.5950 Soluble
140 P L 0.0000 Buried
141 S L -1.6945 Soluble
142 D L -3.0373 Soluble
143 E L -3.2310 Soluble
144 Q L 0.0000 Buried
145 L L -2.2495 Soluble
146 K L -2.8715 Soluble
147 S L -1.7683 Soluble
148 G L -1.3969 Soluble
149 T L -1.0692 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.6109 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.1967 Soluble
162 R L -1.3775 Soluble
163 E L -2.5550 Soluble
164 A L -1.9426 Soluble
165 K L -2.1645 Soluble
166 V L -1.0890 Soluble
167 Q L -0.7784 Soluble
168 W L 0.0000 Buried
169 K L -0.7801 Soluble
170 V L 0.0000 Buried
171 D L -1.5311 Soluble
172 N L -1.2502 Soluble
173 A L -0.4400 Soluble
174 L L 0.2838 Aggregation-prone
175 Q L -0.3378 Soluble
176 S L -0.5005 Soluble
177 G L -0.8144 Soluble
178 N L -0.6785 Soluble
179 S L -0.9082 Soluble
180 Q L -0.9219 Soluble
181 E L -1.4558 Soluble
182 S L -0.4535 Soluble
183 V L -0.1780 Soluble
184 T L -0.7330 Soluble
185 E L -1.8088 Soluble
186 Q L -1.4341 Soluble
187 D L -1.7241 Soluble
188 S L -1.5695 Soluble
189 K L -2.1816 Soluble
190 D L -1.5936 Soluble
191 S L -1.5862 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.5165 Soluble
199 L L 0.0000 Buried
200 T L -0.4072 Soluble
201 L L -0.7086 Soluble
202 S L -1.1285 Soluble
203 K L -2.0756 Soluble
204 A L -1.9066 Soluble
205 D L -2.8507 Soluble
206 Y L 0.0000 Buried
207 E L -3.1204 Soluble
208 K L -3.3683 Soluble
209 H L -2.9580 Soluble
210 K L -3.0688 Soluble
211 V L -1.5503 Soluble
212 Y L 0.0000 Buried
213 A L -0.9373 Soluble
214 C L 0.0000 Buried
215 E L -0.9571 Soluble
216 V L 0.0000 Buried
217 T L -1.1859 Soluble
218 H L 0.0000 Buried
219 Q L -1.5223 Soluble
220 G L -0.4026 Soluble
221 L L -0.2094 Soluble
222 S L -0.4490 Soluble
223 S L -0.4055 Soluble
224 P L -0.4832 Soluble
225 V L 0.0825 Aggregation-prone
226 T L -0.5076 Soluble
227 K L -0.7706 Soluble
228 S L -0.7680 Soluble
229 F L 0.0000 Buried
230 N L -2.3138 Soluble
231 R L -2.7869 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
Load structure to view sequence…
Downloads
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6795 1.8402 CSV PDB
4.5 -0.7269 1.8134 CSV PDB
5.0 -0.7821 1.779 CSV PDB
5.5 -0.8349 1.7417 CSV PDB
6.0 -0.8743 1.7316 CSV PDB
6.5 -0.8917 1.7597 CSV PDB
7.0 -0.8873 1.7952 CSV PDB
7.5 -0.8687 1.8347 CSV PDB
8.0 -0.842 1.8757 CSV PDB
8.5 -0.809 1.917 CSV PDB
9.0 -0.7698 1.9576 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH