Ivonescimab / 1cz8

A4DAB147_1cz8_H_L | Source PDB: 1cz8 | Chains: H, L | Done
97.83
Yes
10 Å
Yes
Avg A4D Score
-0.6603
Max Score
2.4555
Min Score
-3.5341
Total Score
-284.59
Score CDR
-0.0949
Score non-CDR
-0.7345

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGYDFTHYGMNWVRQAPGKGLEWVGWINTYTGEPTYAADFKRRFTFSLDTSKSTAYLQMNSLRAEDTAVYYCAKYPYYYGTSHWYFDVWGQGTLVTVSSASTKGPSVFPLAPSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKDIQLTQSPSSLSASVGDRVTITCSASQDISNYLNWYQQKPGKAPKVLIYFTSSLHSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYSTVPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.5341
Maximum Score 2.4555
Average Score -0.6603
Total Score -284.5875

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.0176 Soluble
2 V H -1.2246 Soluble
3 Q H -0.9348 Soluble
4 L H 0.0000 Buried
5 V H 1.0166 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.4252 Soluble
8 G H -0.9374 Soluble
9 G H -0.6963 Soluble
11 G H -0.3331 Soluble
12 L H -0.1682 Soluble
13 V H 0.0000 Buried
14 Q H -1.7202 Soluble
15 P H -1.9130 Soluble
16 G H -1.6267 Soluble
17 G H -1.3746 Soluble
18 S H -1.6867 Soluble
19 L H -1.4409 Soluble
20 R H -2.3536 Soluble
21 L H 0.0000 Buried
22 S H -0.4274 Soluble
23 C H 0.0000 Buried
24 A H 0.0027 Aggregation-prone
25 A H 0.0000 Buried
26 S H -1.0848 Soluble
27 G H -1.4859 Soluble
28 Y H -1.2316 Soluble
29 D H -1.6959 Soluble
30 F H 0.0000 Buried
35 T H -0.4358 Soluble
36 H H -0.8046 Soluble
37 Y H -0.0365 Soluble
38 G H -0.1188 Soluble
39 M H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H -0.7279 Soluble
44 Q H -1.0318 Soluble
45 A H -1.3073 Soluble
46 P H -1.0147 Soluble
47 G H -1.4658 Soluble
48 K H -2.4038 Soluble
49 G H -1.7084 Soluble
50 L H 0.0000 Buried
51 E H -1.4901 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 G H 0.0000 Buried
55 W H -0.1106 Soluble
56 I H 0.0000 Buried
57 N H -0.6238 Soluble
58 T H 0.0000 Buried
59 Y H 0.6874 Aggregation-prone
62 T H -0.1629 Soluble
63 G H -0.9283 Soluble
64 E H -1.8581 Soluble
65 P H -1.2406 Soluble
66 T H -0.5692 Soluble
67 Y H -0.7612 Soluble
68 A H 0.0000 Buried
69 A H -1.6239 Soluble
70 D H -2.6170 Soluble
71 F H 0.0000 Buried
72 K H -3.1297 Soluble
74 R H -3.3763 Soluble
75 R H -2.5538 Soluble
76 F H 0.0000 Buried
77 T H -1.3810 Soluble
78 F H 0.0000 Buried
79 S H -0.4840 Soluble
80 L H -0.4954 Soluble
81 D H -1.1823 Soluble
82 T H -1.0079 Soluble
83 S H -1.2593 Soluble
84 K H -2.0317 Soluble
85 S H -1.3571 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H 0.0000 Buried
89 L H 0.0000 Buried
90 Q H -1.7002 Soluble
91 M H 0.0000 Buried
92 N H -2.4091 Soluble
93 S H -1.7540 Soluble
94 L H 0.0000 Buried
95 R H -2.6211 Soluble
96 A H -1.8439 Soluble
97 E H -2.2891 Soluble
98 D H 0.0000 Buried
99 T H -0.8336 Soluble
100 A H 0.0000 Buried
101 V H 0.0749 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 K H 0.0000 Buried
107 Y H 0.0000 Buried
108 P H 1.0543 Aggregation-prone
109 Y H 2.0099 Aggregation-prone
110 Y H 2.4555 Aggregation-prone
111 Y H 1.9474 Aggregation-prone
111A G H 0.5888 Aggregation-prone
112B T H 0.5154 Aggregation-prone
112A S H 0.7799 Aggregation-prone
112 H H 1.0417 Aggregation-prone
113 W H 0.7887 Aggregation-prone
114 Y H 0.0000 Buried
115 F H 0.0000 Buried
116 D H -0.2889 Soluble
117 V H -0.3009 Soluble
118 W H 0.0000 Buried
119 G H 0.0000 Buried
120 Q H -1.3156 Soluble
121 G H -0.6398 Soluble
122 T H -0.1024 Soluble
123 L H 0.1203 Aggregation-prone
124 V H 0.0000 Buried
125 T H -0.5663 Soluble
126 V H 0.0000 Buried
127 S H -0.8853 Soluble
128 S H -0.7702 Soluble
129 A H -0.4605 Soluble
130 S H -0.5386 Soluble
131 T H -0.6668 Soluble
132 K H -1.0639 Soluble
133 G H -1.3448 Soluble
134 P H 0.0000 Buried
135 S H -0.3381 Soluble
136 V H 0.0000 Buried
137 F H -0.6065 Soluble
138 P H -1.1826 Soluble
139 L H 0.0000 Buried
140 A H -1.2058 Soluble
141 P H 0.0000 Buried
142 S H -0.6224 Soluble
143 G H -0.6363 Soluble
144 T H -0.5061 Soluble
145 A H -0.2551 Soluble
146 A H 0.0000 Buried
147 L H 0.0000 Buried
148 G H 0.0000 Buried
149 C H 0.0000 Buried
150 L H 0.0000 Buried
151 V H 0.0000 Buried
152 K H 0.0000 Buried
153 D H -0.3741 Soluble
154 Y H 0.0000 Buried
155 F H 0.0000 Buried
156 P H 0.0000 Buried
157 E H -0.4475 Soluble
158 P H -0.6998 Soluble
159 V H -0.7141 Soluble
160 T H -0.6708 Soluble
161 V H -0.4026 Soluble
162 S H -0.4099 Soluble
163 W H 0.0000 Buried
164 N H -0.7763 Soluble
165 S H -0.6574 Soluble
166 G H -0.4528 Soluble
167 A H -0.1969 Soluble
168 L H 0.0230 Aggregation-prone
169 T H -0.1532 Soluble
170 S H -0.1754 Soluble
171 G H -0.1977 Soluble
172 V H 0.1414 Aggregation-prone
173 H H -0.2185 Soluble
174 T H 0.0488 Aggregation-prone
175 F H 0.0000 Buried
176 P H -0.1729 Soluble
177 A H 0.2836 Aggregation-prone
178 V H 0.6334 Aggregation-prone
179 L H 1.1713 Aggregation-prone
180 Q H 0.3127 Aggregation-prone
181 S H -0.1320 Soluble
182 S H -0.1926 Soluble
183 G H 0.0680 Aggregation-prone
184 L H 0.1766 Aggregation-prone
185 Y H 0.5083 Aggregation-prone
186 S H 0.0000 Buried
187 L H 0.0000 Buried
188 S H 0.0000 Buried
189 S H 0.0000 Buried
190 V H 0.0000 Buried
191 V H 0.0000 Buried
192 T H -0.1805 Soluble
193 V H 0.0000 Buried
194 P H -0.5844 Soluble
195 S H -0.5348 Soluble
196 S H -0.5950 Soluble
197 S H -0.5882 Soluble
198 L H -0.8536 Soluble
199 G H -0.9883 Soluble
200 T H -0.8866 Soluble
201 Q H -1.2147 Soluble
202 T H -1.1492 Soluble
203 Y H 0.0000 Buried
204 I H -1.4446 Soluble
205 C H 0.0000 Buried
206 N H -1.5242 Soluble
207 V H 0.0000 Buried
208 N H -2.0814 Soluble
209 H H 0.0000 Buried
210 K H -2.8119 Soluble
211 P H -1.6008 Soluble
212 S H -1.7959 Soluble
213 N H -2.5771 Soluble
214 T H -2.0734 Soluble
215 K H -2.7251 Soluble
216 V H -1.6853 Soluble
217 D H -2.5235 Soluble
218 K H -2.1351 Soluble
219 K H -2.5398 Soluble
220 V H 0.0000 Buried
221 E H -2.8963 Soluble
222 P H -1.9276 Soluble
223 K H -2.4613 Soluble
1 D L -2.0540 Soluble
2 I L 0.0000 Buried
3 Q L -2.0305 Soluble
4 L L 0.0000 Buried
5 T L -0.9077 Soluble
6 Q L 0.0000 Buried
7 S L -0.5782 Soluble
8 P L -0.4842 Soluble
9 S L -0.7397 Soluble
10 S L -0.6472 Soluble
11 L L -0.4579 Soluble
12 S L -0.6626 Soluble
13 A L -0.9978 Soluble
14 S L -0.8964 Soluble
15 V L -0.0179 Soluble
16 G L -0.8294 Soluble
17 D L -1.8046 Soluble
18 R L -2.3969 Soluble
19 V L 0.0000 Buried
20 T L -0.6611 Soluble
21 I L 0.0000 Buried
22 T L -0.5923 Soluble
23 C L 0.0000 Buried
24 S L -1.4793 Soluble
25 A L -1.7592 Soluble
26 S L -2.0216 Soluble
27 Q L -2.7279 Soluble
28 D L -2.7611 Soluble
29 I L 0.0000 Buried
36 S L -1.0906 Soluble
37 N L -0.4759 Soluble
38 Y L 0.4470 Aggregation-prone
39 L L 0.0000 Buried
40 N L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -1.1891 Soluble
45 K L -1.6345 Soluble
46 P L -1.3496 Soluble
47 G L -1.7561 Soluble
48 K L -2.5131 Soluble
49 A L -1.5409 Soluble
50 P L 0.0000 Buried
51 K L -1.3125 Soluble
52 V L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.8515 Aggregation-prone
56 F L 1.2129 Aggregation-prone
57 T L 0.0000 Buried
65 S L 0.1388 Aggregation-prone
66 S L 0.3683 Aggregation-prone
67 L L 0.4016 Aggregation-prone
68 H L -0.2730 Soluble
69 S L -0.3812 Soluble
70 G L -0.5002 Soluble
71 V L -0.2944 Soluble
72 P L -0.3173 Soluble
74 S L -0.3958 Soluble
75 R L -0.6771 Soluble
76 F L 0.0000 Buried
77 S L -0.2601 Soluble
78 G L 0.0000 Buried
79 S L -0.6620 Soluble
80 G L -1.1789 Soluble
83 S L -1.2787 Soluble
84 G L -1.7962 Soluble
85 T L -2.1692 Soluble
86 D L -2.5083 Soluble
87 F L 0.0000 Buried
88 T L -0.6835 Soluble
89 L L 0.0000 Buried
90 T L -0.5930 Soluble
91 I L 0.0000 Buried
92 S L -1.3495 Soluble
93 S L -1.2004 Soluble
94 L L 0.0000 Buried
95 Q L -1.0062 Soluble
96 P L -1.4052 Soluble
97 E L -1.5344 Soluble
98 D L 0.0000 Buried
99 F L 0.0000 Buried
100 A L 0.0000 Buried
101 T L -0.7106 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 Y L 0.0000 Buried
108 S L -0.1361 Soluble
109 T L 0.1108 Aggregation-prone
114 V L 0.6067 Aggregation-prone
115 P L 0.0960 Aggregation-prone
116 W L -0.0780 Soluble
117 T L -0.7436 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 Q L -1.5965 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -1.1365 Soluble
124 V L 0.0000 Buried
125 E L -0.9529 Soluble
126 I L 0.0000 Buried
127 K L -1.3136 Soluble
128 R L -0.8666 Soluble
129 T L 0.0162 Aggregation-prone
130 V L 0.7253 Aggregation-prone
131 A L 0.3241 Aggregation-prone
132 A L -0.0176 Soluble
133 P L 0.0000 Buried
134 S L -0.1332 Soluble
135 V L 0.0000 Buried
136 F L 0.1501 Aggregation-prone
137 I L 0.0759 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.4726 Soluble
140 P L 0.0000 Buried
141 S L -1.7188 Soluble
142 D L -2.9863 Soluble
143 E L -2.6872 Soluble
144 Q L 0.0000 Buried
145 L L -2.2373 Soluble
146 K L -2.7585 Soluble
147 S L -1.6943 Soluble
148 G L -1.1501 Soluble
149 T L -0.8339 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.7072 Soluble
159 F L 0.0000 Buried
160 Y L -0.7364 Soluble
161 P L -1.2414 Soluble
162 R L -2.0512 Soluble
163 E L -2.7829 Soluble
164 A L -2.0614 Soluble
165 K L -2.1897 Soluble
166 V L 0.0000 Buried
167 Q L -1.1134 Soluble
168 W L 0.0000 Buried
169 K L -1.0446 Soluble
170 V L 0.0000 Buried
171 D L -2.1342 Soluble
172 N L -1.7909 Soluble
173 A L -0.6487 Soluble
174 L L -0.0246 Soluble
175 Q L -0.4880 Soluble
176 S L -0.5910 Soluble
177 G L -0.9256 Soluble
178 N L -0.9040 Soluble
179 S L -1.0538 Soluble
180 Q L -1.1439 Soluble
181 E L -1.4204 Soluble
182 S L -0.6246 Soluble
183 V L -0.2495 Soluble
184 T L -0.6873 Soluble
185 E L -1.6004 Soluble
186 Q L 0.0000 Buried
187 D L -1.8363 Soluble
188 S L -2.0557 Soluble
189 K L -2.5533 Soluble
190 D L -1.9898 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.5063 Soluble
199 L L 0.0000 Buried
200 T L -0.3409 Soluble
201 L L -0.6223 Soluble
202 S L -1.1599 Soluble
203 K L -1.9685 Soluble
204 A L -1.7788 Soluble
205 D L -2.3909 Soluble
206 Y L 0.0000 Buried
207 E L -3.3138 Soluble
208 K L -3.5341 Soluble
209 H L -2.9863 Soluble
210 K L -2.9663 Soluble
211 V L -1.2475 Soluble
212 Y L 0.0000 Buried
213 A L -0.9212 Soluble
214 C L 0.0000 Buried
215 E L -1.2927 Soluble
216 V L 0.0000 Buried
217 T L -1.3009 Soluble
218 H L 0.0000 Buried
219 Q L -1.4325 Soluble
220 G L -0.3486 Soluble
221 L L -0.2544 Soluble
222 S L -0.4902 Soluble
223 S L -0.4594 Soluble
224 P L -0.6468 Soluble
225 V L -0.1947 Soluble
226 T L -0.6117 Soluble
227 K L -0.6756 Soluble
228 S L -0.5020 Soluble
229 F L -0.8386 Soluble
230 N L -1.8023 Soluble
231 R L -2.4539 Soluble
232 G L -2.1063 Soluble
233 E L -2.4580 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6725 2.7824 CSV PDB
4.5 -0.7209 2.7827 CSV PDB
5.0 -0.7765 2.7837 CSV PDB
5.5 -0.8293 2.7861 CSV PDB
6.0 -0.8683 2.7906 CSV PDB
6.5 -0.8844 2.7963 CSV PDB
7.0 -0.8774 2.8007 CSV PDB
7.5 -0.8556 2.8029 CSV PDB
8.0 -0.8257 2.8032 CSV PDB
8.5 -0.7889 2.8017 CSV PDB
9.0 -0.7444 2.7961 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH