Erlizumab / 2fgw

A4DAB110_2fgw_H_L | Source PDB: 2fgw | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.796
Max Score
0.9168
Min Score
-4.3874
Total Score
-345.45
Score CDR
-0.6872
Score non-CDR
-0.8248

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCATSGYTFTEYTMHWMRQAPGKGLEWVAGINPKNGGTSHNQRFMDRFTISVDKSTSTAYMQMNSLRAEDTAVYYCARWRRYFDVWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDDIQMTQSPSSLSASVGDRVTITCRASQDINNYLNWYQQKPGKAPKLLIYYTSTLHSGVPSRFSGSGSGTDYTLTISSLQPEDFATYYCQQGNTLPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -4.3874
Maximum Score 0.9168
Average Score -0.796
Total Score -345.4523

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.0605 Soluble
2 V H -1.1777 Soluble
3 Q H -1.3889 Soluble
4 L H 0.0000 Buried
5 V H 0.1306 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.6518 Soluble
8 G H -0.9873 Soluble
9 G H -0.5434 Soluble
11 G H -0.2129 Soluble
12 L H -0.0964 Soluble
13 V H 0.0000 Buried
14 Q H -1.6224 Soluble
15 P H -1.6619 Soluble
16 G H -1.4067 Soluble
17 G H -1.0924 Soluble
18 S H -1.3862 Soluble
19 L H -1.3114 Soluble
20 R H -2.3471 Soluble
21 L H 0.0000 Buried
22 S H -0.5928 Soluble
23 C H 0.0000 Buried
24 A H -0.0074 Soluble
25 T H -0.2361 Soluble
26 S H -0.8372 Soluble
27 G H -1.2396 Soluble
28 Y H -0.7482 Soluble
29 T H -0.5595 Soluble
30 F H 0.0000 Buried
35 T H -2.0170 Soluble
36 E H -2.0484 Soluble
37 Y H -1.1079 Soluble
38 T H 0.0000 Buried
39 M H 0.0000 Buried
40 H H -0.2494 Soluble
41 W H 0.0000 Buried
42 M H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.7218 Soluble
45 A H -1.0924 Soluble
46 P H -0.9331 Soluble
47 G H -1.4736 Soluble
48 K H -2.3811 Soluble
49 G H -1.6046 Soluble
50 L H 0.0000 Buried
51 E H -0.8087 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 A H 0.0000 Buried
55 G H 0.0000 Buried
56 I H 0.0000 Buried
57 N H -1.7635 Soluble
58 P H 0.0000 Buried
59 K H -3.1089 Soluble
62 N H -2.4286 Soluble
63 G H -1.9152 Soluble
64 G H -1.2335 Soluble
65 T H -0.5645 Soluble
66 S H -0.2537 Soluble
67 H H -0.7536 Soluble
68 N H -1.2822 Soluble
69 Q H -2.3176 Soluble
70 R H -2.6797 Soluble
71 F H 0.0000 Buried
72 M H -1.3658 Soluble
74 D H -2.3586 Soluble
75 R H -1.7569 Soluble
76 F H 0.0000 Buried
77 T H -0.7891 Soluble
78 I H -0.2219 Soluble
79 S H -0.4775 Soluble
80 V H -0.7038 Soluble
81 D H -1.5166 Soluble
82 K H -2.3097 Soluble
83 S H -1.2313 Soluble
84 T H -0.8551 Soluble
85 S H -0.8069 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.5895 Soluble
89 M H 0.0000 Buried
90 Q H -1.7122 Soluble
91 M H 0.0000 Buried
92 N H -2.0164 Soluble
93 S H -1.4394 Soluble
94 L H 0.0000 Buried
95 R H -2.1172 Soluble
96 A H -1.6722 Soluble
97 E H -2.1552 Soluble
98 D H 0.0000 Buried
99 T H -0.6616 Soluble
100 A H 0.0000 Buried
101 V H 0.1395 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 W H -0.6467 Soluble
108 R H -1.4755 Soluble
109 R H -0.8488 Soluble
114 Y H -0.2959 Soluble
115 F H 0.0000 Buried
116 D H -0.5689 Soluble
117 V H -0.3825 Soluble
118 W H 0.0000 Buried
119 G H 0.0000 Buried
120 Q H -1.5656 Soluble
121 G H -0.7575 Soluble
122 T H -0.1540 Soluble
123 L H 0.2157 Aggregation-prone
124 V H 0.0000 Buried
125 T H -0.3959 Soluble
126 V H 0.0000 Buried
127 S H -0.6359 Soluble
128 S H -0.7372 Soluble
129 A H -0.4776 Soluble
130 S H -0.5715 Soluble
131 T H -0.6206 Soluble
132 K H -1.3732 Soluble
133 G H -1.3841 Soluble
134 P H -0.7184 Soluble
135 S H -0.4031 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.0052 Soluble
139 L H 0.0000 Buried
140 A H -1.1916 Soluble
141 P H 0.0000 Buried
142 S H -1.5169 Soluble
143 S H -1.7889 Soluble
144 K H -2.4546 Soluble
145 S H -1.3660 Soluble
146 T H -0.9714 Soluble
147 S H -1.1601 Soluble
148 G H -1.3960 Soluble
149 G H -1.0934 Soluble
150 T H -0.7982 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H 0.0000 Buried
159 D H -0.5546 Soluble
160 Y H 0.0000 Buried
161 F H -0.2712 Soluble
162 P H 0.0000 Buried
163 E H -0.4592 Soluble
164 P H -0.4756 Soluble
165 V H 0.0000 Buried
166 T H -0.4162 Soluble
167 V H -0.3546 Soluble
168 S H -0.3353 Soluble
169 W H 0.0000 Buried
170 N H -0.6552 Soluble
171 S H -0.6746 Soluble
172 G H -0.5028 Soluble
173 A H -0.2127 Soluble
174 L H 0.1067 Aggregation-prone
175 T H -0.1281 Soluble
176 S H -0.1260 Soluble
177 G H -0.1430 Soluble
178 V H 0.2223 Aggregation-prone
179 H H -0.2230 Soluble
180 T H 0.0013 Aggregation-prone
181 F H 0.0000 Buried
182 P H -0.3262 Soluble
183 A H 0.1524 Aggregation-prone
184 V H 0.3724 Aggregation-prone
185 L H 0.9168 Aggregation-prone
186 Q H 0.1122 Aggregation-prone
187 S H -0.3393 Soluble
188 S H -0.2715 Soluble
189 G H -0.0541 Soluble
190 L H 0.0197 Aggregation-prone
191 Y H 0.3617 Aggregation-prone
192 S H 0.0000 Buried
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.1058 Soluble
199 V H 0.0000 Buried
200 P H -0.5313 Soluble
201 S H -1.0353 Soluble
202 S H -0.9358 Soluble
203 S H -0.7127 Soluble
204 L H 0.0000 Buried
205 G H -1.1459 Soluble
206 T H -0.9266 Soluble
207 Q H -1.5700 Soluble
208 T H -1.2969 Soluble
209 Y H 0.0000 Buried
210 I H -1.0310 Soluble
211 C H 0.0000 Buried
212 N H -1.4939 Soluble
213 V H 0.0000 Buried
214 N H -1.6164 Soluble
215 H H 0.0000 Buried
216 K H -1.8512 Soluble
217 P H -1.1376 Soluble
218 S H -1.5412 Soluble
219 N H -2.2572 Soluble
220 T H -1.8852 Soluble
221 K H -2.5246 Soluble
222 V H -1.6983 Soluble
223 D H -2.4212 Soluble
224 K H -2.0292 Soluble
225 K H -2.1808 Soluble
226 V H 0.0000 Buried
227 E H -2.7379 Soluble
228 P H -2.2513 Soluble
229 K H -2.6701 Soluble
230 S H -1.7709 Soluble
231 C H -1.9926 Soluble
232 D H -2.9003 Soluble
1 D L -2.0860 Soluble
2 I L 0.0000 Buried
3 Q L -2.0748 Soluble
4 M L 0.0000 Buried
5 T L -1.1677 Soluble
6 Q L 0.0000 Buried
7 S L -0.5785 Soluble
8 P L -0.5135 Soluble
9 S L -0.6733 Soluble
10 S L -0.6233 Soluble
11 L L -0.5852 Soluble
12 S L -1.0524 Soluble
13 A L 0.0000 Buried
14 S L -1.4034 Soluble
15 V L -0.8047 Soluble
16 G L -1.2754 Soluble
17 D L -2.4121 Soluble
18 R L -2.6077 Soluble
19 V L 0.0000 Buried
20 T L -0.5074 Soluble
21 I L 0.0000 Buried
22 T L -0.6564 Soluble
23 C L 0.0000 Buried
24 R L -2.2899 Soluble
25 A L 0.0000 Buried
26 S L -2.2295 Soluble
27 Q L -3.1906 Soluble
28 D L -3.3190 Soluble
29 I L 0.0000 Buried
36 N L -2.2910 Soluble
37 N L -1.5258 Soluble
38 Y L -0.4428 Soluble
39 L L 0.0000 Buried
40 N L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.3080 Soluble
46 P L -1.2594 Soluble
47 G L -1.4685 Soluble
48 K L -2.3514 Soluble
49 A L -1.5216 Soluble
50 P L 0.0000 Buried
51 K L -1.5029 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.4729 Aggregation-prone
56 Y L 0.4191 Aggregation-prone
57 T L 0.0000 Buried
65 S L -0.0608 Soluble
66 T L 0.3338 Aggregation-prone
67 L L 0.5103 Aggregation-prone
68 H L -0.2632 Soluble
69 S L -0.3410 Soluble
70 G L -0.4702 Soluble
71 V L -0.2797 Soluble
72 P L -0.3195 Soluble
74 S L -0.4154 Soluble
75 R L -0.9151 Soluble
76 F L 0.0000 Buried
77 S L -0.3178 Soluble
78 G L -0.1544 Soluble
79 S L -0.6671 Soluble
80 G L -1.3407 Soluble
83 S L -1.7129 Soluble
84 G L -2.3102 Soluble
85 T L -2.5008 Soluble
86 D L -2.3902 Soluble
87 Y L 0.0000 Buried
88 T L -0.6772 Soluble
89 L L 0.0000 Buried
90 T L -0.6704 Soluble
91 I L 0.0000 Buried
92 S L -1.7149 Soluble
93 S L -1.5416 Soluble
94 L L 0.0000 Buried
95 Q L -1.2676 Soluble
96 P L -1.7874 Soluble
97 E L -1.6915 Soluble
98 D L 0.0000 Buried
99 F L 0.0000 Buried
100 A L 0.0000 Buried
101 T L -0.5307 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 G L 0.0000 Buried
108 N L -1.1536 Soluble
109 T L -0.3852 Soluble
114 L L 0.2893 Aggregation-prone
115 P L -0.3870 Soluble
116 P L -0.6150 Soluble
117 T L -0.8349 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 Q L -1.6482 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -0.9195 Soluble
124 V L 0.0000 Buried
125 E L -1.4389 Soluble
126 I L 0.0000 Buried
127 K L -1.7882 Soluble
128 R L -1.2263 Soluble
129 T L -0.1900 Soluble
130 V L 0.4529 Aggregation-prone
131 A L 0.1160 Aggregation-prone
132 A L -0.1277 Soluble
133 P L 0.0000 Buried
134 S L -0.0739 Soluble
135 V L 0.0000 Buried
136 F L 0.0812 Aggregation-prone
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L 0.0000 Buried
140 P L 0.0000 Buried
141 S L -1.6139 Soluble
142 D L -2.9891 Soluble
143 E L -2.5343 Soluble
144 Q L 0.0000 Buried
145 L L 0.0000 Buried
146 K L -2.7769 Soluble
147 S L -1.7135 Soluble
148 G L -1.2118 Soluble
149 T L -1.2888 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.8228 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.8796 Soluble
162 R L -3.0675 Soluble
163 E L -3.2605 Soluble
164 A L -2.3922 Soluble
165 K L -2.6133 Soluble
166 V L -1.6110 Soluble
167 Q L -1.1158 Soluble
168 W L 0.0000 Buried
169 K L -0.6890 Soluble
170 V L 0.0000 Buried
171 D L -1.8746 Soluble
172 N L -1.5901 Soluble
173 A L -0.3999 Soluble
174 L L 0.5995 Aggregation-prone
175 Q L -0.5435 Soluble
176 S L -0.8159 Soluble
177 G L -1.4321 Soluble
178 N L -1.8160 Soluble
179 S L -1.8376 Soluble
180 Q L -1.9631 Soluble
181 E L -2.1119 Soluble
182 S L -0.9391 Soluble
183 V L -0.9264 Soluble
184 T L -1.1754 Soluble
185 E L -2.1654 Soluble
186 Q L 0.0000 Buried
187 D L -2.3584 Soluble
188 S L -2.2718 Soluble
189 K L -2.8488 Soluble
190 D L -2.6433 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.9468 Soluble
199 L L 0.0000 Buried
200 T L -0.7302 Soluble
201 L L -0.8930 Soluble
202 S L -1.2310 Soluble
203 K L -2.5116 Soluble
204 A L -2.1262 Soluble
205 D L -2.9326 Soluble
206 Y L 0.0000 Buried
207 E L -4.3874 Soluble
208 K L -4.1130 Soluble
209 H L -3.4482 Soluble
210 K L -3.4297 Soluble
211 V L -1.3661 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -1.1594 Soluble
216 V L 0.0000 Buried
217 T L -1.3544 Soluble
218 H L 0.0000 Buried
219 Q L -1.6178 Soluble
220 G L -0.5351 Soluble
221 L L -0.3250 Soluble
222 S L -0.5155 Soluble
223 S L -0.4683 Soluble
224 P L -0.6609 Soluble
225 V L -0.0409 Soluble
226 T L -0.4413 Soluble
227 K L -0.4138 Soluble
228 S L -0.2937 Soluble
229 F L -0.5001 Soluble
230 N L -1.8842 Soluble
231 R L -3.0769 Soluble
232 G L 0.0000 Buried
233 E L -3.8708 Soluble
234 C L -2.8672 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.8124 1.3665 CSV PDB
4.5 -0.8638 1.2763 CSV PDB
5.0 -0.9242 1.1705 CSV PDB
5.5 -0.983 1.1105 CSV PDB
6.0 -1.0284 1.1067 CSV PDB
6.5 -1.0501 1.1067 CSV PDB
7.0 -1.0475 1.1067 CSV PDB
7.5 -1.0292 1.1868 CSV PDB
8.0 -1.0023 1.3314 CSV PDB
8.5 -0.9684 1.48 CSV PDB
9.0 -0.9265 1.6294 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH