Izalontamab / 5t1l

A4DAB149_5t1l_D_C | Source PDB: 5t1l | Chains: D, C | Done
99.56
Yes
10 Å
Yes
Avg A4D Score
-0.66
Max Score
1.9787
Min Score
-3.7014
Total Score
-280.48
Score CDR
-0.295
Score non-CDR
-0.7043

Calculation Parameters

Chain Sequence(s) QVQLKQSGPGLVQPSQSLSITCTVSGFSLTNYGVHWVRQSPGKGLEWLGVIWSGGNTDYNTPFTSRLSINKDNSKSQVFFKMNSLQNDTAIYYCARALTYYDYEFAYWGQGTLVTVSAASTKGPSVFPLAPSSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKDILLTQSPVILSVSPGERVSFSCRASQSIGTNIHWYQQRTNGSPRLLIKYASESISGIPSRFSGSGSGTDFTLSINSVESEDIADYYCQQNNNWPTTFGAGTKLELKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGA input.pdb
Selected Chain(s) D (Heavy), C (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.7014
Maximum Score 1.9787
Average Score -0.66
Total Score -280.4794

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q D -1.4284 Soluble
2 V D -0.7989 Soluble
3 Q D -1.5475 Soluble
4 L D 0.0000 Buried
5 K D -2.0980 Soluble
6 Q D 0.0000 Buried
7 S D -0.8505 Soluble
8 G D -1.0337 Soluble
9 P D -0.3617 Soluble
11 G D -0.2468 Soluble
12 L D -0.0326 Soluble
13 V D 0.0000 Buried
14 Q D -1.6842 Soluble
15 P D -1.5471 Soluble
16 S D -1.5941 Soluble
17 Q D -1.9902 Soluble
18 S D -1.9217 Soluble
19 L D 0.0000 Buried
20 S D -0.8975 Soluble
21 I D 0.0000 Buried
22 T D -0.5301 Soluble
23 C D 0.0000 Buried
24 T D -1.0522 Soluble
25 V D 0.0000 Buried
26 S D -1.1821 Soluble
27 G D -0.9706 Soluble
28 F D -0.5436 Soluble
29 S D -0.8719 Soluble
30 L D 0.0000 Buried
35 T D -0.8996 Soluble
36 N D -0.8422 Soluble
37 Y D 0.4858 Aggregation-prone
38 G D 0.0000 Buried
39 V D 0.0000 Buried
40 H D 0.0000 Buried
41 W D 0.0000 Buried
42 V D 0.0000 Buried
43 R D 0.0000 Buried
44 Q D -0.4807 Soluble
45 S D -1.0465 Soluble
46 P D -1.2220 Soluble
47 G D -1.7790 Soluble
48 K D -2.2476 Soluble
49 G D -1.1770 Soluble
50 L D 0.0000 Buried
51 E D 0.0000 Buried
52 W D 0.0000 Buried
53 L D 0.0000 Buried
54 G D 0.0000 Buried
55 V D 0.0000 Buried
56 I D 0.0000 Buried
57 W D -0.5107 Soluble
58 S D -0.6125 Soluble
59 G D -1.2210 Soluble
63 G D -1.5365 Soluble
64 N D -1.8644 Soluble
65 T D -1.3290 Soluble
66 D D -1.0321 Soluble
67 Y D -0.3899 Soluble
68 N D 0.0000 Buried
69 T D -0.2900 Soluble
70 P D -0.3769 Soluble
71 F D 0.0000 Buried
72 T D -0.4971 Soluble
74 S D -0.7147 Soluble
75 R D -1.3748 Soluble
76 L D 0.0000 Buried
77 S D -1.3226 Soluble
78 I D 0.0000 Buried
79 N D -1.7468 Soluble
80 K D -1.3607 Soluble
81 D D -1.7035 Soluble
82 N D -2.1459 Soluble
83 S D -1.6522 Soluble
84 K D -2.3386 Soluble
85 S D -1.5802 Soluble
86 Q D -1.4385 Soluble
87 V D 0.0000 Buried
88 F D -0.2910 Soluble
89 F D 0.0000 Buried
90 K D -2.0929 Soluble
91 M D 0.0000 Buried
92 N D -2.3642 Soluble
94 L D 0.0000 Buried
95 Q D -2.1253 Soluble
97 N D -1.9709 Soluble
98 D D 0.0000 Buried
99 T D -0.5354 Soluble
100 A D 0.0000 Buried
101 I D 0.4058 Aggregation-prone
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 A D 0.0000 Buried
106 R D 0.0000 Buried
107 A D 0.0000 Buried
108 L D 1.0614 Aggregation-prone
109 T D 1.1693 Aggregation-prone
110 Y D 1.8834 Aggregation-prone
111 Y D 1.9787 Aggregation-prone
112 D D 0.0000 Buried
113 Y D 0.6119 Aggregation-prone
114 E D 0.0000 Buried
115 F D 0.0000 Buried
116 A D 0.2175 Aggregation-prone
117 Y D 0.1330 Aggregation-prone
118 W D 0.0000 Buried
119 G D 0.0000 Buried
120 Q D -1.8054 Soluble
121 G D -0.9166 Soluble
122 T D 0.0000 Buried
123 L D 0.3167 Aggregation-prone
124 V D 0.0000 Buried
125 T D 0.0000 Buried
126 V D -0.7537 Soluble
127 S D -0.4990 Soluble
128 A D -0.3794 Soluble
129 A D -0.3173 Soluble
130 S D -0.5553 Soluble
131 T D -0.6028 Soluble
132 K D -1.2641 Soluble
133 G D -1.3328 Soluble
134 P D 0.0000 Buried
135 S D -0.4088 Soluble
136 V D 0.0000 Buried
137 F D 0.0000 Buried
138 P D -1.2763 Soluble
139 L D 0.0000 Buried
140 A D -1.2079 Soluble
141 P D -0.8213 Soluble
142 S D -0.9730 Soluble
143 S D -0.4425 Soluble
144 G D -0.8688 Soluble
145 G D -0.8495 Soluble
146 T D -0.6048 Soluble
147 A D 0.0000 Buried
148 A D 0.0000 Buried
149 L D 0.0000 Buried
150 G D 0.0000 Buried
151 C D 0.0000 Buried
152 L D 0.0000 Buried
153 V D 0.0000 Buried
154 K D 0.0000 Buried
155 D D -0.4097 Soluble
156 Y D 0.0000 Buried
157 F D 0.0000 Buried
158 P D 0.0000 Buried
159 E D -0.0593 Soluble
160 P D -0.3510 Soluble
161 V D -0.4803 Soluble
162 T D -0.4724 Soluble
163 V D -0.1926 Soluble
164 S D -0.3725 Soluble
165 W D 0.0000 Buried
166 N D -0.7308 Soluble
167 S D -0.6252 Soluble
168 G D -0.5265 Soluble
169 A D -0.2265 Soluble
170 L D 0.0308 Aggregation-prone
171 T D -0.1446 Soluble
172 S D -0.1513 Soluble
173 G D -0.1674 Soluble
174 V D 0.1782 Aggregation-prone
175 H D -0.3208 Soluble
176 T D -0.0098 Soluble
177 F D 0.0000 Buried
178 P D -0.5240 Soluble
179 A D 0.2714 Aggregation-prone
180 V D 0.5541 Aggregation-prone
181 L D 1.3123 Aggregation-prone
182 Q D 0.3610 Aggregation-prone
183 S D -0.0148 Soluble
184 S D -0.1836 Soluble
185 G D 0.0957 Aggregation-prone
186 L D 0.2129 Aggregation-prone
187 Y D 0.5364 Aggregation-prone
188 S D 0.0000 Buried
189 L D 0.0000 Buried
190 S D 0.0000 Buried
191 S D 0.0000 Buried
192 V D 0.0000 Buried
193 V D 0.0000 Buried
194 T D -0.1136 Soluble
195 V D 0.0000 Buried
196 P D -0.6158 Soluble
197 S D -0.6218 Soluble
198 S D -0.6400 Soluble
199 S D -0.5725 Soluble
200 L D -0.8678 Soluble
201 G D -0.9801 Soluble
202 T D -0.6867 Soluble
203 Q D -1.1683 Soluble
204 T D -1.1496 Soluble
205 Y D 0.0000 Buried
206 I D -1.2795 Soluble
207 C D 0.0000 Buried
208 N D -1.3675 Soluble
209 V D 0.0000 Buried
210 N D -1.8507 Soluble
211 H D 0.0000 Buried
212 K D -2.2058 Soluble
213 P D -1.3181 Soluble
214 S D -1.6657 Soluble
215 N D -2.3495 Soluble
216 T D -1.9596 Soluble
217 K D -2.5706 Soluble
218 V D -1.5704 Soluble
219 D D -2.1214 Soluble
220 K D -1.9940 Soluble
221 R D -2.5536 Soluble
222 V D 0.0000 Buried
223 E D -2.8569 Soluble
224 P D -1.9269 Soluble
225 K D -2.4224 Soluble
1 D C -1.4072 Soluble
2 I C 0.0000 Buried
3 L C 0.6320 Aggregation-prone
4 L C 0.0000 Buried
5 T C -0.3440 Soluble
6 Q C 0.0000 Buried
7 S C 0.1106 Aggregation-prone
8 P C 0.8436 Aggregation-prone
9 V C 1.9280 Aggregation-prone
10 I C 1.4462 Aggregation-prone
11 L C 0.5543 Aggregation-prone
12 S C -0.2442 Soluble
13 V C 0.0000 Buried
14 S C -1.9089 Soluble
15 P C -2.1284 Soluble
16 G C -2.2058 Soluble
17 E C -3.0221 Soluble
18 R C -3.1928 Soluble
19 V C 0.0000 Buried
20 S C -0.9126 Soluble
21 F C 0.0000 Buried
23 C C 0.0000 Buried
24 R C -2.3829 Soluble
25 A C 0.0000 Buried
26 S C -0.9245 Soluble
27 Q C -1.4000 Soluble
28 S C -1.4281 Soluble
29 I C 0.0000 Buried
36 G C -0.9693 Soluble
37 T C -0.7597 Soluble
38 N C -0.8198 Soluble
39 I C 0.0000 Buried
40 H C 0.0000 Buried
41 W C 0.0000 Buried
42 Y C 0.0000 Buried
43 Q C 0.0000 Buried
44 Q C -1.2510 Soluble
45 R C -1.6325 Soluble
46 T C -1.6094 Soluble
47 N C -1.7604 Soluble
48 G C -1.4663 Soluble
49 S C -1.0168 Soluble
50 P C 0.0000 Buried
51 R C -0.8763 Soluble
52 L C 0.0000 Buried
53 L C 0.0000 Buried
54 I C 0.0000 Buried
55 K C -0.7638 Soluble
56 Y C -0.5996 Soluble
57 A C 0.0000 Buried
65 S C -1.2977 Soluble
66 E C -1.9892 Soluble
67 S C -1.1834 Soluble
68 I C -0.5025 Soluble
69 S C -0.3358 Soluble
70 G C -0.4726 Soluble
71 I C -0.2838 Soluble
72 P C -0.3288 Soluble
74 S C -0.5393 Soluble
75 R C -1.0438 Soluble
76 F C 0.0000 Buried
77 S C -0.8804 Soluble
78 G C -0.7720 Soluble
79 S C -0.8565 Soluble
80 G C -1.0629 Soluble
83 S C -0.8876 Soluble
84 G C -1.2139 Soluble
85 T C -1.7967 Soluble
86 D C -2.3359 Soluble
87 F C 0.0000 Buried
88 T C -0.8530 Soluble
89 L C 0.0000 Buried
90 S C -0.8609 Soluble
91 I C 0.0000 Buried
92 N C -2.1130 Soluble
93 S C -2.1875 Soluble
94 V C 0.0000 Buried
95 E C -2.4510 Soluble
96 S C -1.9479 Soluble
98 D C 0.0000 Buried
99 I C 0.0000 Buried
100 A C 0.0000 Buried
101 D C -0.8713 Soluble
102 Y C 0.0000 Buried
103 Y C 0.0000 Buried
104 C C 0.0000 Buried
105 Q C 0.0000 Buried
106 Q C 0.0000 Buried
107 N C -0.6209 Soluble
108 N C -1.2539 Soluble
109 N C -0.9466 Soluble
114 W C 0.1893 Aggregation-prone
115 P C 0.0000 Buried
116 T C 0.0000 Buried
117 T C -0.0009 Soluble
118 F C 0.2152 Aggregation-prone
119 G C 0.0000 Buried
120 A C 0.0264 Aggregation-prone
121 G C 0.0954 Aggregation-prone
122 T C 0.0000 Buried
123 K C -0.2606 Soluble
124 L C 0.0000 Buried
125 E C -1.1308 Soluble
126 L C 0.0000 Buried
127 K C -1.6749 Soluble
128 R C -1.3400 Soluble
129 T C -0.2700 Soluble
130 V C 0.1140 Aggregation-prone
131 A C 0.0263 Aggregation-prone
132 A C -0.1550 Soluble
133 P C 0.0000 Buried
134 S C -0.1712 Soluble
135 V C 0.0000 Buried
136 F C 0.1085 Aggregation-prone
137 I C 0.0529 Aggregation-prone
138 F C 0.0000 Buried
139 P C -0.5076 Soluble
140 P C -0.7599 Soluble
141 S C 0.0000 Buried
142 D C -2.9763 Soluble
143 E C -2.7568 Soluble
144 Q C 0.0000 Buried
145 L C -2.1915 Soluble
146 K C -2.6877 Soluble
147 S C -1.6596 Soluble
148 G C -1.1916 Soluble
149 T C -0.8358 Soluble
150 A C 0.0000 Buried
151 S C 0.0000 Buried
152 V C 0.0000 Buried
153 V C 0.0000 Buried
154 C C 0.0000 Buried
155 L C 0.0000 Buried
156 L C 0.0000 Buried
157 N C 0.0000 Buried
158 N C -0.9281 Soluble
159 F C 0.0000 Buried
160 Y C 0.0000 Buried
161 P C -1.8268 Soluble
162 R C -3.1935 Soluble
163 E C -3.2923 Soluble
164 A C -2.3793 Soluble
165 K C -2.5086 Soluble
166 V C -1.2996 Soluble
167 Q C -0.7906 Soluble
168 W C 0.0000 Buried
169 K C -0.5793 Soluble
170 V C 0.0000 Buried
171 D C -1.9601 Soluble
172 N C -1.5479 Soluble
173 A C -0.2644 Soluble
174 L C 0.6389 Aggregation-prone
175 Q C -0.1674 Soluble
176 S C -0.4229 Soluble
177 G C -0.8978 Soluble
178 N C -0.8159 Soluble
179 S C -1.1937 Soluble
180 Q C -1.4166 Soluble
181 E C -2.0015 Soluble
182 S C -1.0857 Soluble
183 V C -0.9704 Soluble
184 T C -1.2317 Soluble
185 E C -2.3287 Soluble
186 Q C 0.0000 Buried
187 D C -1.8947 Soluble
188 S C -1.9335 Soluble
189 K C -2.4570 Soluble
190 D C -1.8596 Soluble
191 S C 0.0000 Buried
192 T C 0.0000 Buried
193 Y C 0.0000 Buried
194 S C 0.0000 Buried
195 L C 0.0000 Buried
196 S C 0.0000 Buried
197 S C 0.0000 Buried
198 T C -0.6780 Soluble
199 L C 0.0000 Buried
200 T C -0.3127 Soluble
201 L C -0.6830 Soluble
202 S C -1.0467 Soluble
203 K C -2.0114 Soluble
204 A C -1.9367 Soluble
205 D C -2.6179 Soluble
206 Y C 0.0000 Buried
207 E C -3.5842 Soluble
208 K C -3.7014 Soluble
209 H C -3.2354 Soluble
210 K C -3.1763 Soluble
211 V C -1.4738 Soluble
212 Y C 0.0000 Buried
213 A C 0.0000 Buried
214 C C 0.0000 Buried
215 E C -0.9994 Soluble
216 V C 0.0000 Buried
217 T C -1.2736 Soluble
218 H C 0.0000 Buried
219 Q C -1.8054 Soluble
220 G C -0.4675 Soluble
221 L C -0.2662 Soluble
222 S C -0.4673 Soluble
223 S C -0.4458 Soluble
224 P C -0.5930 Soluble
225 V C -0.1190 Soluble
226 T C -0.5250 Soluble
227 K C -0.7513 Soluble
228 S C -0.6027 Soluble
229 F C -0.7624 Soluble
230 N C -1.7533 Soluble
231 R C -2.2418 Soluble
232 G C -1.4278 Soluble
233 A C -1.0178 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6683 2.7452 CSV PDB
4.5 -0.7028 2.686 CSV PDB
5.0 -0.7438 2.6125 CSV PDB
5.5 -0.7829 2.5326 CSV PDB
6.0 -0.8101 2.4506 CSV PDB
6.5 -0.8173 2.3678 CSV PDB
7.0 -0.8047 2.285 CSV PDB
7.5 -0.7789 2.2028 CSV PDB
8.0 -0.7458 2.1992 CSV PDB
8.5 -0.7078 2.1972 CSV PDB
9.0 -0.6654 2.1907 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH