Fulranumab / 4m6o

A4DAB121_4m6o_H_L | Source PDB: 4m6o | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6875
Max Score
1.5367
Min Score
-3.6163
Total Score
-301.13
Score CDR
-0.3464
Score non-CDR
-0.7315

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFTLRSYSMNWVRQAPGKGLEWVSYISRSSHTIFYADSVKGRFTISRDNAKNSLYLQMDSLRDEDTAMYYCARVYSSGWHVSDYFDYWGQGILVTVSSASTKGPSVFPLAPSSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCHHHAIQLTQSPSSLSASVGDRVTITCRASQGISSALAWYQQKPGKAPKLLIYDASSLESGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQFNSYPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.6163
Maximum Score 1.5367
Average Score -0.6875
Total Score -301.1312

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -1.9768 Soluble
2 V H -0.8064 Soluble
3 Q H -0.9376 Soluble
4 L H 0.0000 Buried
5 V H 0.8847 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.3764 Soluble
8 G H -0.8009 Soluble
9 G H -0.4188 Soluble
11 G H -0.2746 Soluble
12 L H -0.1514 Soluble
13 V H -0.6896 Soluble
14 Q H -1.8151 Soluble
15 P H -1.8599 Soluble
16 G H -1.5690 Soluble
17 G H -1.0434 Soluble
18 S H -1.2774 Soluble
19 L H -1.1219 Soluble
20 R H -2.1861 Soluble
21 L H 0.0000 Buried
22 S H -0.5028 Soluble
23 C H 0.0000 Buried
24 A H -0.1679 Soluble
25 A H 0.0000 Buried
26 S H -0.8288 Soluble
27 G H -1.0333 Soluble
28 F H -0.6079 Soluble
29 T H -0.9141 Soluble
30 L H 0.0000 Buried
35 R H -2.7207 Soluble
36 S H -1.1813 Soluble
37 Y H -0.6616 Soluble
38 S H 0.0000 Buried
39 M H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.8401 Soluble
45 A H -1.2411 Soluble
46 P H -1.0370 Soluble
47 G H -1.5618 Soluble
48 K H -2.3004 Soluble
49 G H -1.4961 Soluble
50 L H 0.0000 Buried
51 E H -1.0868 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 Y H 0.8554 Aggregation-prone
56 I H 0.0000 Buried
57 S H -0.8785 Soluble
58 R H -2.1363 Soluble
59 S H -1.5419 Soluble
62 S H -1.2944 Soluble
63 H H -1.1378 Soluble
64 T H 0.0278 Aggregation-prone
65 I H 1.3602 Aggregation-prone
66 F H 1.1098 Aggregation-prone
67 Y H -0.2396 Soluble
68 A H -1.2391 Soluble
69 D H -2.4046 Soluble
70 S H -1.7776 Soluble
71 V H 0.0000 Buried
72 K H -2.5049 Soluble
74 G H -1.7526 Soluble
75 R H -1.4036 Soluble
76 F H 0.0000 Buried
77 T H -0.7454 Soluble
78 I H 0.0000 Buried
79 S H -0.3800 Soluble
80 R H -1.4595 Soluble
81 D H -1.8988 Soluble
82 N H -2.2679 Soluble
83 A H -1.5717 Soluble
84 K H -2.4151 Soluble
85 N H -1.9277 Soluble
86 S H 0.0000 Buried
87 L H 0.0000 Buried
88 Y H 0.0000 Buried
89 L H 0.0000 Buried
90 Q H -1.6014 Soluble
91 M H 0.0000 Buried
92 D H -1.5056 Soluble
93 S H -1.1835 Soluble
94 L H 0.0000 Buried
95 R H -3.0248 Soluble
96 D H -3.4731 Soluble
97 E H -3.0450 Soluble
98 D H 0.0000 Buried
99 T H -1.2378 Soluble
100 A H 0.0000 Buried
101 M H 0.1397 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 V H 0.0000 Buried
108 Y H 0.8026 Aggregation-prone
109 S H 0.3606 Aggregation-prone
110 S H 0.2557 Aggregation-prone
111 G H 0.1668 Aggregation-prone
111A W H 0.8241 Aggregation-prone
112B H H 0.2790 Aggregation-prone
112A V H 1.1496 Aggregation-prone
112 S H 0.4120 Aggregation-prone
113 D H 0.0905 Aggregation-prone
114 Y H 0.0000 Buried
115 F H 0.0000 Buried
116 D H 0.0015 Aggregation-prone
117 Y H 0.2494 Aggregation-prone
118 W H -0.3040 Soluble
119 G H 0.0000 Buried
120 Q H -1.3210 Soluble
121 G H -0.5109 Soluble
122 I H 0.2149 Aggregation-prone
123 L H 0.2799 Aggregation-prone
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -1.1398 Soluble
128 S H -0.8803 Soluble
129 A H -0.5443 Soluble
130 S H -0.7300 Soluble
131 T H -0.8680 Soluble
132 K H -1.5154 Soluble
133 G H -1.6254 Soluble
134 P H 0.0000 Buried
135 S H -0.3824 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H 0.0000 Buried
139 L H 0.0000 Buried
140 A H -1.0125 Soluble
141 P H 0.0000 Buried
142 S H -0.8043 Soluble
143 S H -0.2335 Soluble
144 G H -0.6490 Soluble
145 T H -0.4737 Soluble
146 A H -0.2787 Soluble
147 A H 0.0000 Buried
148 L H 0.0000 Buried
149 G H 0.0000 Buried
150 C H 0.0000 Buried
151 L H 0.0000 Buried
152 V H 0.0000 Buried
153 K H 0.0000 Buried
154 D H -0.4254 Soluble
155 Y H 0.0000 Buried
156 F H 0.0000 Buried
157 P H 0.0000 Buried
158 E H -0.6544 Soluble
159 P H -0.8027 Soluble
160 V H -0.8797 Soluble
161 T H -0.7417 Soluble
162 V H -0.2793 Soluble
163 S H -0.4296 Soluble
164 W H 0.0000 Buried
165 N H -0.6371 Soluble
166 S H -0.5699 Soluble
167 G H -0.5223 Soluble
168 A H -0.2633 Soluble
169 L H -0.0432 Soluble
170 T H -0.2107 Soluble
171 S H -0.2268 Soluble
172 G H -0.2947 Soluble
173 V H 0.1307 Aggregation-prone
174 H H -0.3663 Soluble
175 T H -0.1790 Soluble
176 F H 0.0000 Buried
177 P H -0.4948 Soluble
178 A H 0.2187 Aggregation-prone
179 V H 0.6428 Aggregation-prone
180 L H 1.5367 Aggregation-prone
181 Q H 0.4458 Aggregation-prone
182 S H -0.1269 Soluble
183 S H -0.1530 Soluble
184 G H 0.1099 Aggregation-prone
185 L H 0.1641 Aggregation-prone
186 Y H 0.5081 Aggregation-prone
187 S H 0.0000 Buried
188 L H 0.0000 Buried
189 S H 0.0000 Buried
190 S H 0.0000 Buried
191 V H 0.0000 Buried
192 V H 0.0000 Buried
193 T H -0.1474 Soluble
194 V H 0.0000 Buried
195 P H -0.5047 Soluble
196 S H -0.6698 Soluble
197 S H -0.6143 Soluble
198 S H -0.6362 Soluble
199 L H -0.7808 Soluble
200 G H -0.9999 Soluble
201 T H -0.7273 Soluble
202 Q H -1.2098 Soluble
203 T H -1.0597 Soluble
204 Y H 0.0000 Buried
205 I H -0.8999 Soluble
206 C H 0.0000 Buried
207 N H -1.3394 Soluble
208 V H 0.0000 Buried
209 N H -2.4026 Soluble
210 H H 0.0000 Buried
211 K H -2.9067 Soluble
212 P H -1.7155 Soluble
213 S H -1.9867 Soluble
214 N H -2.5884 Soluble
215 T H -2.2316 Soluble
216 K H -2.7756 Soluble
217 V H -1.3683 Soluble
218 D H -1.8587 Soluble
219 K H -1.5608 Soluble
220 K H -2.2690 Soluble
221 V H 0.0000 Buried
222 E H -2.5530 Soluble
223 P H -1.5662 Soluble
224 K H -2.1277 Soluble
225 S H -1.2281 Soluble
226 C H -0.8002 Soluble
227 H H -1.4359 Soluble
228 H H -1.7444 Soluble
229 H H -1.5598 Soluble
1 A L -0.3187 Soluble
2 I L -0.8771 Soluble
3 Q L -1.6096 Soluble
4 L L 0.0000 Buried
5 T L -1.1704 Soluble
6 Q L -0.9806 Soluble
7 S L -0.6431 Soluble
8 P L -0.6688 Soluble
9 S L -0.9492 Soluble
10 S L -0.9988 Soluble
11 L L -0.6868 Soluble
12 S L -0.8087 Soluble
13 A L 0.0000 Buried
14 S L -0.8501 Soluble
15 V L -0.2366 Soluble
16 G L -0.9026 Soluble
17 D L -1.8854 Soluble
18 R L -2.3418 Soluble
19 V L 0.0000 Buried
20 T L -0.6336 Soluble
21 I L 0.0000 Buried
22 T L -0.7043 Soluble
23 C L 0.0000 Buried
24 R L -2.3373 Soluble
25 A L 0.0000 Buried
26 S L -1.6554 Soluble
27 Q L -2.1064 Soluble
28 G L -1.6382 Soluble
29 I L 0.0000 Buried
36 S L -1.1117 Soluble
37 S L -0.9836 Soluble
38 A L 0.0000 Buried
39 L L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.8865 Soluble
46 P L -1.5175 Soluble
47 G L -1.7027 Soluble
48 K L -2.5919 Soluble
49 A L -1.6423 Soluble
50 P L 0.0000 Buried
51 K L -1.8407 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.5167 Soluble
56 D L -1.0346 Soluble
57 A L 0.0000 Buried
65 S L -0.6939 Soluble
66 S L -0.4086 Soluble
67 L L -0.0754 Soluble
68 E L -0.5667 Soluble
69 S L -0.4813 Soluble
70 G L -0.5588 Soluble
71 V L -0.4308 Soluble
72 P L -0.3544 Soluble
74 S L -0.3783 Soluble
75 R L -0.7580 Soluble
76 F L 0.0000 Buried
77 S L -0.3015 Soluble
78 G L -0.4454 Soluble
79 S L -0.7668 Soluble
80 G L -1.0692 Soluble
83 S L -0.9860 Soluble
84 G L -1.2342 Soluble
85 T L -1.6313 Soluble
86 D L -1.8907 Soluble
87 F L 0.0000 Buried
88 T L -0.6607 Soluble
89 L L 0.0000 Buried
90 T L -0.5783 Soluble
91 I L 0.0000 Buried
92 S L -1.3677 Soluble
93 S L -1.2030 Soluble
94 L L 0.0000 Buried
95 Q L -1.0982 Soluble
96 P L -1.7344 Soluble
97 E L -2.3636 Soluble
98 D L 0.0000 Buried
99 F L 0.0000 Buried
100 A L 0.0000 Buried
101 T L -1.0120 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 F L 0.0000 Buried
108 N L -0.8654 Soluble
109 S L -0.2546 Soluble
114 Y L 0.1077 Aggregation-prone
115 P L -0.1518 Soluble
116 L L 0.0000 Buried
117 T L -0.4593 Soluble
118 F L -0.3649 Soluble
119 G L 0.0000 Buried
120 G L -1.2733 Soluble
121 G L -1.0853 Soluble
122 T L 0.0000 Buried
123 K L -1.9662 Soluble
124 V L 0.0000 Buried
125 E L -1.2221 Soluble
126 I L 0.0000 Buried
127 K L -1.0022 Soluble
128 R L -0.6226 Soluble
129 T L 0.2030 Aggregation-prone
130 V L 1.2092 Aggregation-prone
131 A L 0.4398 Aggregation-prone
132 A L 0.0549 Aggregation-prone
133 P L 0.0000 Buried
134 S L -0.0727 Soluble
135 V L 0.0550 Aggregation-prone
136 F L 0.1923 Aggregation-prone
137 I L 0.1272 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.4443 Soluble
140 P L 0.0000 Buried
141 S L 0.0000 Buried
142 D L -1.8901 Soluble
143 E L -1.8094 Soluble
144 Q L 0.0000 Buried
145 L L -1.6388 Soluble
146 K L -2.3628 Soluble
147 S L -1.4778 Soluble
148 G L -1.1393 Soluble
149 T L -0.9077 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.9218 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.5085 Soluble
162 R L -2.7003 Soluble
163 E L -3.1299 Soluble
164 A L -2.3496 Soluble
165 K L -2.4422 Soluble
166 V L -1.1557 Soluble
167 Q L -0.6417 Soluble
168 W L 0.0000 Buried
169 K L -0.3984 Soluble
170 V L 0.0000 Buried
171 D L -1.5557 Soluble
172 N L -1.3561 Soluble
173 A L -0.0974 Soluble
174 L L 0.7912 Aggregation-prone
175 Q L -0.3398 Soluble
176 S L -0.6901 Soluble
177 G L -1.3319 Soluble
178 N L -1.7337 Soluble
179 S L -1.4792 Soluble
180 Q L -1.3244 Soluble
181 E L -1.8190 Soluble
182 S L -0.9168 Soluble
183 V L -0.6802 Soluble
184 T L -1.0900 Soluble
185 E L -2.0690 Soluble
186 Q L 0.0000 Buried
187 D L -2.3152 Soluble
188 S L -2.4487 Soluble
189 K L -2.6971 Soluble
190 D L -2.0121 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.7204 Soluble
199 L L 0.0000 Buried
200 T L -0.6440 Soluble
201 L L -0.8341 Soluble
202 S L -1.0797 Soluble
203 K L -2.0362 Soluble
204 A L -1.9787 Soluble
205 D L -2.8041 Soluble
206 Y L 0.0000 Buried
207 E L -3.5874 Soluble
208 K L -3.6163 Soluble
209 H L -3.1281 Soluble
210 K L -3.0340 Soluble
211 V L -1.3433 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.7727 Soluble
216 V L 0.0000 Buried
217 T L -1.2756 Soluble
218 H L 0.0000 Buried
219 Q L -1.6019 Soluble
220 G L -0.2243 Soluble
221 L L -0.1463 Soluble
222 S L -0.4816 Soluble
223 S L -0.4435 Soluble
224 P L -0.5939 Soluble
225 V L -0.0905 Soluble
226 T L -0.5413 Soluble
227 K L -0.8101 Soluble
228 S L -0.6566 Soluble
229 F L -1.0881 Soluble
230 N L -2.0887 Soluble
231 R L -2.3736 Soluble
232 G L -2.0674 Soluble
233 E L -2.1454 Soluble
234 C L -0.8511 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7938 2.4707 CSV PDB
4.5 -0.8329 2.4257 CSV PDB
5.0 -0.8772 2.3804 CSV PDB
5.5 -0.9168 2.34 CSV PDB
6.0 -0.9409 2.3286 CSV PDB
6.5 -0.9419 2.449 CSV PDB
7.0 -0.9221 2.5699 CSV PDB
7.5 -0.8901 2.6475 CSV PDB
8.0 -0.8515 2.6822 CSV PDB
8.5 -0.8072 2.6947 CSV PDB
9.0 -0.7569 2.6989 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH