Eblasakimab / 4hwb

A4DAB095_4hwb_H_L | Source PDB: 4hwb | Chains: H, L | Done
97.75
Yes
10 Å
Yes
Avg A4D Score
-0.7023
Max Score
0.9054
Min Score
-3.7452
Total Score
-299.88
Score CDR
-0.2865
Score non-CDR
-0.7477

Calculation Parameters

Chain Sequence(s) EVQLVQSGAEVKKPGESLKISCKGSGYSFTSYWIGWVRQMPGKGLEWMGVIYPGDSYTRYSPSFQGQVTISADKSISTAYLQWSSLKASDTAMYYCARMPNWGSFDYWGQGTLVTVSSASTKGPSVFPLAPSSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKEVVLTQSPGTLSLSPGERATLSCRASQSISSSYLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYETFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGECA input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.7452
Maximum Score 0.9054
Average Score -0.7023
Total Score -299.8797

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.1146 Soluble
2 V H -1.3199 Soluble
3 Q H -1.4118 Soluble
4 L H 0.0000 Buried
5 V H 0.1280 Aggregation-prone
6 Q H 0.0000 Buried
7 S H -0.5296 Soluble
8 G H -0.5124 Soluble
9 A H 0.0890 Aggregation-prone
11 E H -0.2421 Soluble
12 V H 0.7769 Aggregation-prone
13 K H -1.1465 Soluble
14 K H -2.4292 Soluble
15 P H -2.0363 Soluble
16 G H -1.9784 Soluble
17 E H -2.1826 Soluble
18 S H -1.7068 Soluble
19 L H 0.0000 Buried
20 K H -1.7471 Soluble
21 I H 0.0000 Buried
22 S H -0.5544 Soluble
23 C H 0.0000 Buried
24 K H -0.6176 Soluble
25 G H 0.0000 Buried
26 S H -0.7506 Soluble
27 G H -1.2419 Soluble
28 Y H -0.5355 Soluble
29 S H -0.3582 Soluble
30 F H 0.0000 Buried
35 T H -0.8059 Soluble
36 S H -0.1280 Soluble
37 Y H 0.1244 Aggregation-prone
38 W H 0.2732 Aggregation-prone
39 I H 0.0000 Buried
40 G H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H -0.6824 Soluble
44 Q H -0.8122 Soluble
45 M H -0.7808 Soluble
46 P H -0.8749 Soluble
47 G H -1.4322 Soluble
48 K H -2.3241 Soluble
49 G H -1.5104 Soluble
50 L H 0.0000 Buried
51 E H -1.1480 Soluble
52 W H 0.0000 Buried
53 M H 0.0000 Buried
54 G H 0.0000 Buried
55 V H 0.0000 Buried
56 I H 0.0000 Buried
57 Y H -0.2175 Soluble
58 P H 0.0000 Buried
59 G H -1.1878 Soluble
62 D H -1.6984 Soluble
63 S H -0.8385 Soluble
64 Y H 0.4635 Aggregation-prone
65 T H -0.0952 Soluble
66 R H -1.0006 Soluble
67 Y H -1.0110 Soluble
68 S H 0.0000 Buried
69 P H -1.2329 Soluble
70 S H -0.8599 Soluble
71 F H 0.0000 Buried
72 Q H -1.8702 Soluble
74 G H -1.4160 Soluble
75 Q H -1.4457 Soluble
76 V H 0.0000 Buried
77 T H -0.8879 Soluble
78 I H 0.0000 Buried
79 S H -0.4185 Soluble
80 A H -0.7977 Soluble
81 D H -1.2213 Soluble
82 K H -1.8787 Soluble
83 S H -0.3337 Soluble
84 I H 0.9054 Aggregation-prone
85 S H -0.3074 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.6030 Soluble
89 L H 0.0000 Buried
90 Q H -1.2102 Soluble
91 W H 0.0000 Buried
92 S H -1.0368 Soluble
93 S H -1.5045 Soluble
94 L H 0.0000 Buried
95 K H -2.1828 Soluble
96 A H -1.2181 Soluble
97 S H -0.6688 Soluble
98 D H 0.0000 Buried
99 T H -0.1049 Soluble
100 A H 0.0000 Buried
101 M H 0.2550 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 M H 0.0000 Buried
108 P H 0.0000 Buried
109 N H -0.7362 Soluble
110 W H 0.2875 Aggregation-prone
113 G H 0.0000 Buried
114 S H 0.0000 Buried
115 F H 0.0000 Buried
116 D H -0.3624 Soluble
117 Y H -0.3363 Soluble
118 W H -0.3247 Soluble
119 G H 0.0000 Buried
120 Q H -1.1607 Soluble
121 G H -0.4855 Soluble
122 T H 0.0000 Buried
123 L H 0.6833 Aggregation-prone
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -0.8634 Soluble
128 S H -1.1696 Soluble
129 A H -0.7365 Soluble
130 S H -0.7657 Soluble
131 T H -0.6694 Soluble
132 K H -1.2707 Soluble
133 G H -1.5215 Soluble
134 P H 0.0000 Buried
135 S H -0.4462 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.1661 Soluble
139 L H 0.0000 Buried
140 A H -1.1869 Soluble
141 P H 0.0000 Buried
142 S H -0.5907 Soluble
143 S H -0.4235 Soluble
144 G H -0.7844 Soluble
145 G H -0.8304 Soluble
146 T H -0.6086 Soluble
147 A H 0.0000 Buried
148 A H 0.0000 Buried
149 L H 0.0000 Buried
150 G H 0.0000 Buried
151 C H 0.0000 Buried
152 L H 0.0000 Buried
153 V H 0.0000 Buried
154 K H 0.0000 Buried
155 D H -0.4919 Soluble
156 Y H 0.0000 Buried
157 F H 0.0000 Buried
158 P H 0.0000 Buried
159 E H -0.5522 Soluble
160 P H -0.9229 Soluble
161 V H -0.9065 Soluble
162 T H -0.7824 Soluble
163 V H -0.4501 Soluble
164 S H -0.4415 Soluble
165 W H 0.0000 Buried
166 N H -0.6941 Soluble
167 S H -0.6428 Soluble
168 G H -0.5840 Soluble
169 A H -0.2740 Soluble
170 L H -0.0683 Soluble
171 T H -0.1938 Soluble
172 S H -0.1932 Soluble
173 G H -0.2078 Soluble
174 V H 0.1839 Aggregation-prone
175 H H -0.3052 Soluble
176 T H 0.0141 Aggregation-prone
177 F H 0.0000 Buried
178 P H -0.4291 Soluble
179 A H 0.0386 Aggregation-prone
180 V H 0.1774 Aggregation-prone
181 L H 0.7861 Aggregation-prone
182 Q H 0.0561 Aggregation-prone
183 S H -0.1421 Soluble
184 S H -0.2796 Soluble
185 G H -0.0671 Soluble
186 L H -0.0886 Soluble
187 Y H 0.0000 Buried
188 S H 0.1215 Aggregation-prone
189 L H 0.0000 Buried
190 S H 0.0000 Buried
191 S H 0.0000 Buried
192 V H 0.0000 Buried
193 V H 0.0000 Buried
194 T H -0.1255 Soluble
195 V H 0.0000 Buried
196 P H -0.4693 Soluble
197 S H -0.5582 Soluble
198 S H -0.6066 Soluble
199 S H -0.5907 Soluble
200 L H -0.7395 Soluble
201 G H -1.0744 Soluble
202 T H -0.8010 Soluble
203 Q H -1.2748 Soluble
204 T H -1.2742 Soluble
205 Y H 0.0000 Buried
206 I H -1.1176 Soluble
207 C H 0.0000 Buried
208 N H -1.5155 Soluble
209 V H 0.0000 Buried
210 N H -2.4789 Soluble
211 H H 0.0000 Buried
212 K H -2.8808 Soluble
213 P H -1.7231 Soluble
214 S H -1.8844 Soluble
215 N H -2.6621 Soluble
216 T H -2.2360 Soluble
217 K H -2.8435 Soluble
218 V H -1.5480 Soluble
219 D H -2.4080 Soluble
220 K H -2.0229 Soluble
221 K H -2.6611 Soluble
222 V H 0.0000 Buried
223 E H -2.7544 Soluble
224 P H -1.9527 Soluble
225 K H -2.1951 Soluble
1 E L -1.5851 Soluble
2 V L -0.2415 Soluble
3 V L 0.7585 Aggregation-prone
4 L L 0.0000 Buried
5 T L -0.3931 Soluble
6 Q L 0.0000 Buried
7 S L -0.6004 Soluble
8 P L -0.4396 Soluble
9 G L -0.8148 Soluble
10 T L -0.3689 Soluble
11 L L -0.1742 Soluble
12 S L -0.6302 Soluble
13 L L -1.0627 Soluble
14 S L -1.6779 Soluble
15 P L -2.1062 Soluble
16 G L -2.5848 Soluble
17 E L -3.0001 Soluble
18 R L -3.0811 Soluble
19 A L 0.0000 Buried
20 T L -0.5257 Soluble
21 L L 0.0000 Buried
22 S L -0.8633 Soluble
23 C L 0.0000 Buried
24 R L -1.9141 Soluble
25 A L 0.0000 Buried
26 S L -0.8835 Soluble
27 Q L -1.4209 Soluble
28 S L -1.2376 Soluble
29 I L -0.5366 Soluble
30 S L -0.2846 Soluble
36 S L -0.2342 Soluble
37 S L 0.1789 Aggregation-prone
38 Y L 0.5613 Aggregation-prone
39 L L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -0.8535 Soluble
45 K L -1.3174 Soluble
46 P L -1.1930 Soluble
47 G L -1.2600 Soluble
48 Q L -1.9775 Soluble
49 A L -1.3051 Soluble
50 P L 0.0000 Buried
51 R L -1.2098 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.0771 Aggregation-prone
56 G L -0.0234 Soluble
57 A L 0.0000 Buried
65 S L -0.6456 Soluble
66 S L -0.6377 Soluble
67 R L -1.3793 Soluble
68 A L -0.7402 Soluble
69 T L -0.5297 Soluble
70 G L -0.7854 Soluble
71 I L -0.8776 Soluble
72 P L -1.2075 Soluble
74 D L -2.3133 Soluble
75 R L -1.9408 Soluble
76 F L 0.0000 Buried
77 S L -0.8876 Soluble
78 G L -0.5066 Soluble
79 S L -0.7620 Soluble
80 G L -1.1854 Soluble
83 S L -1.1450 Soluble
84 G L -1.2160 Soluble
85 T L -1.8328 Soluble
86 D L -2.4646 Soluble
87 F L 0.0000 Buried
88 T L -0.8323 Soluble
89 L L 0.0000 Buried
90 T L -0.7845 Soluble
91 I L 0.0000 Buried
92 S L -2.4278 Soluble
93 R L -3.6084 Soluble
94 L L 0.0000 Buried
95 E L -2.7656 Soluble
96 P L -1.9217 Soluble
97 E L -2.2961 Soluble
98 D L 0.0000 Buried
99 F L -0.6568 Soluble
100 A L 0.0000 Buried
101 V L -0.1159 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.3481 Aggregation-prone
107 Y L -0.0630 Soluble
116 E L -0.9583 Soluble
117 T L -0.3251 Soluble
118 F L -0.0802 Soluble
119 G L 0.0000 Buried
120 Q L -1.3953 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -0.6293 Soluble
124 V L 0.0000 Buried
125 E L 0.0000 Buried
126 I L -0.6399 Soluble
127 K L -1.4566 Soluble
128 R L -1.0765 Soluble
129 T L -0.1396 Soluble
130 V L 0.5669 Aggregation-prone
131 A L 0.0116 Aggregation-prone
132 A L -0.1201 Soluble
133 P L 0.0000 Buried
134 S L -0.1184 Soluble
135 V L 0.0000 Buried
136 F L 0.0909 Aggregation-prone
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.4983 Soluble
140 P L 0.0000 Buried
141 S L -1.6917 Soluble
142 D L -3.0278 Soluble
143 E L -3.0950 Soluble
144 Q L 0.0000 Buried
145 L L 0.0000 Buried
146 K L -2.8714 Soluble
147 S L -1.8099 Soluble
148 G L -1.2940 Soluble
149 T L -0.8910 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.9413 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.6154 Soluble
162 R L -2.9222 Soluble
163 E L -3.0903 Soluble
164 A L -2.3070 Soluble
165 K L -2.2967 Soluble
166 V L 0.0000 Buried
167 Q L -0.5622 Soluble
168 W L 0.0000 Buried
169 K L -0.5139 Soluble
170 V L 0.0000 Buried
171 D L -1.5822 Soluble
172 N L -1.2756 Soluble
173 A L -0.1867 Soluble
174 L L 0.7816 Aggregation-prone
175 Q L -0.1078 Soluble
176 S L -0.4239 Soluble
177 G L -0.9969 Soluble
178 N L -0.9486 Soluble
179 S L -1.3611 Soluble
180 Q L -1.8881 Soluble
181 E L -2.2118 Soluble
182 S L 0.0000 Buried
183 V L -0.8498 Soluble
184 T L -1.1898 Soluble
185 E L -2.2526 Soluble
186 Q L -1.8838 Soluble
187 D L -2.0529 Soluble
188 S L -1.8002 Soluble
189 K L -2.3743 Soluble
190 D L -1.9550 Soluble
191 S L -1.8159 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.8466 Soluble
199 L L 0.0000 Buried
200 T L -0.4906 Soluble
201 L L -0.6703 Soluble
202 S L -1.1270 Soluble
203 K L -2.2226 Soluble
204 A L -1.8924 Soluble
205 D L -2.5262 Soluble
206 Y L 0.0000 Buried
207 E L -3.7452 Soluble
208 K L -3.6076 Soluble
209 H L -2.9475 Soluble
210 K L -3.0409 Soluble
211 V L -1.5083 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.6557 Soluble
216 V L 0.0000 Buried
217 T L -1.2361 Soluble
218 H L 0.0000 Buried
219 Q L -1.5525 Soluble
220 G L -0.4448 Soluble
221 L L -0.2671 Soluble
222 S L -0.4865 Soluble
223 S L -0.4461 Soluble
224 P L -0.5528 Soluble
225 V L 0.0545 Aggregation-prone
226 T L -0.3957 Soluble
227 K L -0.6191 Soluble
228 S L -0.7101 Soluble
229 F L -1.1136 Soluble
230 N L -2.1549 Soluble
231 R L -2.7859 Soluble
232 G L -2.1686 Soluble
233 E L -2.2631 Soluble
234 C L -0.9522 Soluble
235 A L -0.6713 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7242 1.7563 CSV PDB
4.5 -0.7683 1.7563 CSV PDB
5.0 -0.8206 1.7563 CSV PDB
5.5 -0.8706 1.7563 CSV PDB
6.0 -0.9063 1.7563 CSV PDB
6.5 -0.9179 1.7563 CSV PDB
7.0 -0.9037 1.7563 CSV PDB
7.5 -0.8714 1.7563 CSV PDB
8.0 -0.8289 1.7562 CSV PDB
8.5 -0.7794 1.756 CSV PDB
9.0 -0.7238 1.7553 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH