Sutacimig / 8cn9

A4DAB249_8cn9_Q_P | Source PDB: 8cn9 | Chains: Q, P | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6936
Max Score
1.0654
Min Score
-3.7571
Total Score
-295.49
Score CDR
-0.4051
Score non-CDR
-0.7311

Calculation Parameters

Chain Sequence(s) QVQLQESGPGLVKPSQTLSLTCTVSGYSISSDSAWSWIRQPPGKGLEWIGYIQYSGSTNYNPSLKSRVTISRDTSKNQFSLKLSSVTAADTAVYYCARSVNYYGNSFAVGYWGQGTLVTVSSASTKGPSVFPLAPCSRSTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVEIVLTQSPATLSLSPGERATLSCRASQGISDYLHWYQQKPGEAPRLLIKYTSQPATGIPARFSGSGSGTDFTLTISSLEPEDFAVYYCQNGHSFPLTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) Q (Heavy), P (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.7571
Maximum Score 1.0654
Average Score -0.6936
Total Score -295.4854

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q Q -1.4204 Soluble
2 V Q -0.8571 Soluble
3 Q Q -1.6615 Soluble
4 L Q -1.3341 Soluble
5 Q Q -1.9416 Soluble
6 E Q -1.2631 Soluble
7 S Q -0.8311 Soluble
8 G Q -0.7610 Soluble
9 P Q -0.5919 Soluble
11 G Q 0.0000 Buried
12 L Q -0.5404 Soluble
13 V Q 0.0000 Buried
14 K Q -2.1961 Soluble
15 P Q -1.4973 Soluble
16 S Q -1.3665 Soluble
17 Q Q -1.9306 Soluble
18 T Q -1.3509 Soluble
19 L Q 0.0000 Buried
20 S Q -0.7688 Soluble
21 L Q 0.0000 Buried
22 T Q -0.7282 Soluble
23 C Q 0.0000 Buried
24 T Q -1.2208 Soluble
25 V Q 0.0000 Buried
26 S Q -1.1764 Soluble
27 G Q -0.7788 Soluble
28 Y Q -0.2489 Soluble
29 S Q -0.6053 Soluble
30 I Q 0.0000 Buried
31 S Q -0.7526 Soluble
35 S Q -0.9476 Soluble
36 D Q -1.7265 Soluble
37 S Q 0.0000 Buried
38 A Q 0.0000 Buried
39 W Q 0.0000 Buried
40 S Q 0.0000 Buried
41 W Q 0.0000 Buried
42 I Q 0.0000 Buried
43 R Q 0.0000 Buried
44 Q Q 0.0000 Buried
45 P Q -0.7207 Soluble
46 P Q -0.7740 Soluble
47 G Q -1.2423 Soluble
48 K Q -1.9066 Soluble
49 G Q -1.5358 Soluble
50 L Q 0.0000 Buried
51 E Q -1.3963 Soluble
52 W Q 0.0000 Buried
53 I Q 0.0000 Buried
54 G Q 0.0000 Buried
55 Y Q 0.0000 Buried
56 I Q 0.0000 Buried
57 Q Q -0.6357 Soluble
58 Y Q -0.4466 Soluble
59 S Q -0.5293 Soluble
63 G Q -0.6006 Soluble
64 S Q -0.5121 Soluble
65 T Q -0.5038 Soluble
66 N Q -0.8232 Soluble
67 Y Q -0.7632 Soluble
68 N Q -1.0975 Soluble
69 P Q -1.1318 Soluble
70 S Q -0.7156 Soluble
71 L Q -0.8421 Soluble
72 K Q -1.8039 Soluble
74 S Q -1.1086 Soluble
75 R Q -1.0741 Soluble
76 V Q 0.0000 Buried
77 T Q -0.8496 Soluble
78 I Q 0.0000 Buried
79 S Q -0.8060 Soluble
80 R Q -1.2042 Soluble
81 D Q -1.6823 Soluble
82 T Q -1.3634 Soluble
83 S Q -1.4888 Soluble
84 K Q -2.3399 Soluble
85 N Q -1.6697 Soluble
86 Q Q -1.5282 Soluble
87 F Q 0.0000 Buried
88 S Q -0.7003 Soluble
89 L Q 0.0000 Buried
90 K Q -1.2180 Soluble
91 L Q 0.0000 Buried
92 S Q -0.9888 Soluble
93 S Q -1.0011 Soluble
94 V Q 0.0000 Buried
95 T Q -0.5661 Soluble
96 A Q -0.2178 Soluble
97 A Q 0.0556 Aggregation-prone
98 D Q 0.0000 Buried
99 T Q 0.0798 Aggregation-prone
100 A Q 0.0000 Buried
101 V Q 0.3650 Aggregation-prone
102 Y Q 0.0000 Buried
103 Y Q 0.0000 Buried
104 C Q 0.0000 Buried
105 A Q 0.0000 Buried
106 R Q -0.0104 Soluble
107 S Q 0.0000 Buried
108 V Q -0.0397 Soluble
109 N Q -0.4717 Soluble
110 Y Q 0.0000 Buried
111 Y Q 0.3605 Aggregation-prone
111A G Q -0.7290 Soluble
112A N Q -1.2142 Soluble
112 S Q 0.0000 Buried
113 F Q 0.0000 Buried
114 A Q 0.0000 Buried
115 V Q 0.0000 Buried
116 G Q -0.0321 Soluble
117 Y Q 0.3287 Aggregation-prone
118 W Q -0.6126 Soluble
119 G Q 0.0000 Buried
120 Q Q -2.0956 Soluble
121 G Q -1.1730 Soluble
122 T Q -0.6354 Soluble
123 L Q 0.0139 Aggregation-prone
124 V Q 0.0000 Buried
125 T Q 0.0000 Buried
126 V Q 0.0000 Buried
127 S Q -0.7090 Soluble
128 S Q -0.7960 Soluble
129 A Q -0.5447 Soluble
130 S Q -0.6621 Soluble
131 T Q -0.6487 Soluble
132 K Q -1.1664 Soluble
133 G Q -1.4241 Soluble
134 P Q 0.0000 Buried
135 S Q -0.5085 Soluble
136 V Q 0.0000 Buried
137 F Q -0.4488 Soluble
138 P Q 0.0000 Buried
139 L Q 0.0000 Buried
140 A Q 0.4773 Aggregation-prone
141 P Q -0.0607 Soluble
142 C Q -0.3403 Soluble
143 S Q -1.0091 Soluble
144 R Q -1.7222 Soluble
145 S Q -0.5999 Soluble
146 T Q -0.5479 Soluble
147 A Q -0.3102 Soluble
148 A Q 0.0000 Buried
149 L Q 0.0000 Buried
150 G Q 0.0000 Buried
151 C Q 0.0000 Buried
152 L Q 0.0000 Buried
153 V Q 0.0000 Buried
154 K Q 0.0000 Buried
155 D Q -0.5956 Soluble
156 Y Q 0.0000 Buried
157 F Q 0.0000 Buried
158 P Q 0.0000 Buried
159 E Q -0.3678 Soluble
160 P Q -0.6699 Soluble
161 V Q 0.0000 Buried
162 T Q -0.5688 Soluble
163 V Q 0.0000 Buried
164 S Q -0.4696 Soluble
165 W Q 0.0000 Buried
166 N Q -0.9237 Soluble
167 S Q -0.7604 Soluble
168 G Q -0.5909 Soluble
169 A Q -0.2130 Soluble
170 L Q -0.0388 Soluble
171 T Q -0.1904 Soluble
172 S Q -0.2273 Soluble
173 G Q -0.2241 Soluble
174 V Q 0.1070 Aggregation-prone
175 H Q -0.1629 Soluble
176 T Q -0.0036 Soluble
177 F Q 0.0000 Buried
178 P Q -0.2502 Soluble
179 A Q 0.2448 Aggregation-prone
180 V Q 0.4209 Aggregation-prone
181 L Q 1.0654 Aggregation-prone
182 Q Q 0.0534 Aggregation-prone
183 S Q -0.3149 Soluble
184 S Q -0.2429 Soluble
185 G Q 0.0152 Aggregation-prone
186 L Q 0.1752 Aggregation-prone
187 Y Q 0.4680 Aggregation-prone
188 S Q 0.0000 Buried
189 L Q 0.0000 Buried
190 S Q 0.0000 Buried
191 S Q 0.0000 Buried
192 V Q 0.0000 Buried
193 V Q 0.0000 Buried
194 T Q -0.1234 Soluble
195 V Q 0.0000 Buried
196 P Q -0.2617 Soluble
197 S Q -0.4779 Soluble
198 S Q -0.3086 Soluble
199 S Q -0.2394 Soluble
200 L Q 0.3849 Aggregation-prone
201 G Q -0.3454 Soluble
202 T Q -0.6406 Soluble
203 K Q -1.0201 Soluble
204 T Q -0.7096 Soluble
205 Y Q 0.0000 Buried
206 T Q -1.4063 Soluble
207 C Q 0.0000 Buried
208 N Q -1.8118 Soluble
209 V Q 0.0000 Buried
210 D Q -1.9285 Soluble
211 H Q 0.0000 Buried
212 K Q -2.5036 Soluble
213 P Q -1.5418 Soluble
214 S Q -1.7835 Soluble
215 N Q -2.3467 Soluble
216 T Q -1.8870 Soluble
217 K Q -2.7074 Soluble
218 V Q -1.6949 Soluble
219 D Q -2.8380 Soluble
220 K Q -2.0095 Soluble
221 R Q -1.8062 Soluble
222 V Q 0.4917 Aggregation-prone
1 E P -1.5038 Soluble
2 I P 0.0000 Buried
3 V P 0.6204 Aggregation-prone
4 L P 0.0000 Buried
5 T P -0.8520 Soluble
6 Q P 0.0000 Buried
7 S P -0.8117 Soluble
8 P P -0.3112 Soluble
9 A P -0.4393 Soluble
10 T P -0.2878 Soluble
11 L P -0.0487 Soluble
12 S P -0.3682 Soluble
13 L P -0.8947 Soluble
14 S P -1.5016 Soluble
15 P P -1.8427 Soluble
16 G P -1.6693 Soluble
17 E P -2.2839 Soluble
18 R P -2.4989 Soluble
19 A P 0.0000 Buried
20 T P -0.5680 Soluble
21 L P 0.0000 Buried
22 S P -1.0857 Soluble
23 C P 0.0000 Buried
24 R P -2.5978 Soluble
25 A P 0.0000 Buried
26 S P -1.1601 Soluble
27 Q P -1.9709 Soluble
28 G P -1.7334 Soluble
29 I P 0.0000 Buried
36 S P -1.0077 Soluble
37 D P -0.8523 Soluble
38 Y P 0.0109 Aggregation-prone
39 L P 0.0000 Buried
40 H P 0.0000 Buried
41 W P 0.0000 Buried
42 Y P 0.0000 Buried
43 Q P 0.0000 Buried
44 Q P -1.1187 Soluble
45 K P -1.6623 Soluble
46 P P -1.4729 Soluble
47 G P -1.8013 Soluble
48 E P -2.5034 Soluble
49 A P -1.4954 Soluble
50 P P 0.0000 Buried
51 R P -1.0974 Soluble
52 L P -0.3080 Soluble
53 L P 0.0000 Buried
54 I P 0.0000 Buried
55 K P -0.6108 Soluble
56 Y P -0.3048 Soluble
57 T P 0.0000 Buried
65 S P -1.0504 Soluble
66 Q P -1.3849 Soluble
67 P P -0.8213 Soluble
68 A P -0.5553 Soluble
69 T P -0.3773 Soluble
70 G P -0.4042 Soluble
71 I P -0.2145 Soluble
72 P P -0.2280 Soluble
74 A P -0.1987 Soluble
75 R P -0.4483 Soluble
76 F P 0.0000 Buried
77 S P -0.5463 Soluble
78 G P -0.5605 Soluble
79 S P -0.9060 Soluble
80 G P -1.1268 Soluble
83 S P -1.1615 Soluble
84 G P -1.5862 Soluble
85 T P -2.1988 Soluble
86 D P -2.9451 Soluble
87 F P 0.0000 Buried
88 T P -0.9195 Soluble
89 L P 0.0000 Buried
90 T P -0.6121 Soluble
91 I P 0.0000 Buried
92 S P -1.3255 Soluble
93 S P -1.4602 Soluble
94 L P 0.0000 Buried
95 E P -1.8821 Soluble
96 P P -2.3753 Soluble
97 E P -2.8118 Soluble
98 D P 0.0000 Buried
99 F P 0.0000 Buried
100 A P 0.0000 Buried
101 V P 0.0000 Buried
102 Y P 0.0000 Buried
103 Y P 0.0000 Buried
104 C P 0.0000 Buried
105 Q P 0.0000 Buried
106 N P 0.0000 Buried
107 G P 0.0000 Buried
108 H P -0.6963 Soluble
109 S P -0.6654 Soluble
114 F P -0.4300 Soluble
115 P P -0.6489 Soluble
116 L P 0.0000 Buried
117 T P -0.4045 Soluble
118 F P 0.0000 Buried
119 G P 0.0000 Buried
120 Q P -1.3790 Soluble
121 G P -1.1570 Soluble
122 T P 0.0000 Buried
123 K P -1.0522 Soluble
124 L P 0.0000 Buried
125 E P 0.0000 Buried
126 I P 0.0000 Buried
127 K P -1.4533 Soluble
128 R P -1.1405 Soluble
129 T P -0.1408 Soluble
130 V P 0.5178 Aggregation-prone
131 A P 0.0980 Aggregation-prone
132 A P -0.1154 Soluble
133 P P 0.0000 Buried
134 S P -0.1640 Soluble
135 V P 0.0000 Buried
136 F P 0.1450 Aggregation-prone
137 I P 0.1888 Aggregation-prone
138 F P 0.0000 Buried
139 P P -0.2821 Soluble
140 P P 0.0000 Buried
141 S P -1.5966 Soluble
142 D P -2.9693 Soluble
143 E P -3.2230 Soluble
144 Q P 0.0000 Buried
145 L P -2.1899 Soluble
146 K P -2.8509 Soluble
147 S P -1.7261 Soluble
148 G P -1.2968 Soluble
149 T P -0.9979 Soluble
150 A P 0.0000 Buried
151 S P 0.0000 Buried
152 V P 0.0000 Buried
153 V P 0.0000 Buried
154 C P 0.0000 Buried
155 L P 0.0000 Buried
156 L P 0.0000 Buried
157 N P 0.0000 Buried
158 N P -1.0381 Soluble
159 F P 0.0000 Buried
160 Y P -0.9741 Soluble
161 P P -1.4734 Soluble
162 R P -2.4692 Soluble
163 E P -2.9259 Soluble
164 A P 0.0000 Buried
165 K P -2.7318 Soluble
166 V P -1.7286 Soluble
167 Q P -1.8031 Soluble
168 W P -1.2011 Soluble
169 K P -1.7150 Soluble
170 V P -1.6401 Soluble
171 D P -2.6787 Soluble
172 N P -2.1427 Soluble
173 S P -1.1518 Soluble
174 G P -1.3237 Soluble
175 N P -1.9710 Soluble
176 S P -1.7612 Soluble
177 Q P -1.9521 Soluble
178 E P -2.2923 Soluble
179 S P 0.0000 Buried
180 V P -0.1110 Soluble
181 T P -0.7512 Soluble
182 E P -2.0327 Soluble
183 Q P 0.0000 Buried
184 D P -2.1762 Soluble
185 S P -2.4380 Soluble
186 K P -2.7022 Soluble
187 D P -2.1346 Soluble
188 S P 0.0000 Buried
189 T P 0.0000 Buried
190 Y P 0.0000 Buried
191 S P 0.0000 Buried
192 L P 0.0000 Buried
193 S P 0.0000 Buried
194 S P 0.0000 Buried
195 T P -0.8887 Soluble
196 L P 0.0000 Buried
197 T P -0.6546 Soluble
198 L P -0.6861 Soluble
199 S P -1.1368 Soluble
200 K P -1.9976 Soluble
201 A P -1.8860 Soluble
202 D P -2.4580 Soluble
203 Y P 0.0000 Buried
204 E P -3.7571 Soluble
205 K P -3.7020 Soluble
206 H P -3.4292 Soluble
207 K P -2.9965 Soluble
208 V P -1.3544 Soluble
209 Y P 0.0000 Buried
210 A P 0.0000 Buried
211 C P 0.0000 Buried
212 E P -1.2567 Soluble
213 V P 0.0000 Buried
214 T P -0.9144 Soluble
215 H P 0.0000 Buried
216 Q P -1.3194 Soluble
217 G P -0.4217 Soluble
218 L P -0.1955 Soluble
219 S P -0.4198 Soluble
220 S P -0.3047 Soluble
221 P P -0.1894 Soluble
222 V P 0.3266 Aggregation-prone
223 T P -0.5473 Soluble
224 K P -0.7731 Soluble
225 S P -0.5069 Soluble
226 F P -0.6805 Soluble
227 N P -1.3981 Soluble
228 R P -2.0046 Soluble
229 G P -1.7922 Soluble
230 E P -2.1594 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7298 1.4056 CSV PDB
4.5 -0.7667 1.4056 CSV PDB
5.0 -0.8124 1.4056 CSV PDB
5.5 -0.8571 1.4056 CSV PDB
6.0 -0.8896 1.4145 CSV PDB
6.5 -0.9012 1.4831 CSV PDB
7.0 -0.8917 1.5714 CSV PDB
7.5 -0.8675 1.6686 CSV PDB
8.0 -0.835 1.7689 CSV PDB
8.5 -0.7972 1.87 CSV PDB
9.0 -0.754 1.9703 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH