Nesfrotamig / 1fve

A4DAB178_1fve_D_C | Source PDB: 1fve | Chains: D, C | Done
99.56
Yes
10 Å
Yes
Avg A4D Score
-0.7307
Max Score
1.5069
Min Score
-4.0114
Total Score
-319.3
Score CDR
-0.5117
Score non-CDR
-0.7571

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCSRWGGDGFYAMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDIQMTQSPSSLSASVGDRVTITCRASQDVNTAVAWYQQKPGKAPKLLIYSASFLESGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) D (Heavy), C (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -4.0114
Maximum Score 1.5069
Average Score -0.7307
Total Score -319.3026

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E D -2.0674 Soluble
2 V D -1.2266 Soluble
3 Q D -0.9983 Soluble
4 L D 0.0000 Buried
5 V D 0.9029 Aggregation-prone
6 E D 0.0000 Buried
7 S D -0.2821 Soluble
8 G D -0.7235 Soluble
9 G D -0.1726 Soluble
11 G D 0.1309 Aggregation-prone
12 L D 0.1468 Aggregation-prone
13 V D -0.5136 Soluble
14 Q D -1.4397 Soluble
15 P D -1.4894 Soluble
16 G D -1.2830 Soluble
17 G D -0.9633 Soluble
18 S D -1.1783 Soluble
19 L D -1.0980 Soluble
20 R D -1.9486 Soluble
21 L D 0.0000 Buried
22 S D -0.3295 Soluble
23 C D 0.0000 Buried
24 A D -0.1050 Soluble
25 A D 0.0000 Buried
26 S D -0.9473 Soluble
27 G D -1.3734 Soluble
28 F D -1.2745 Soluble
29 N D -2.0319 Soluble
30 I D 0.0000 Buried
35 K D -2.0246 Soluble
36 D D -2.6467 Soluble
37 T D 0.0000 Buried
38 Y D -0.4983 Soluble
39 I D 0.0000 Buried
40 H D 0.0000 Buried
41 W D 0.0000 Buried
42 V D 0.0000 Buried
43 R D 0.0000 Buried
44 Q D -0.8838 Soluble
45 A D -1.2579 Soluble
46 P D -1.0875 Soluble
47 G D -1.4816 Soluble
48 K D -2.4073 Soluble
49 G D -1.6976 Soluble
50 L D 0.0000 Buried
51 E D -1.4378 Soluble
52 W D 0.0000 Buried
53 V D 0.0000 Buried
54 A D 0.0000 Buried
55 R D -0.5343 Soluble
56 I D 0.0000 Buried
57 Y D -0.4016 Soluble
58 P D 0.0000 Buried
59 T D -1.1215 Soluble
62 N D -1.2289 Soluble
63 G D -0.3512 Soluble
64 Y D 0.4302 Aggregation-prone
65 T D -0.4569 Soluble
66 R D -1.9916 Soluble
67 Y D -1.9084 Soluble
68 A D -1.8805 Soluble
69 D D -2.6327 Soluble
70 S D -1.4726 Soluble
71 V D 0.0000 Buried
72 K D -2.8227 Soluble
74 G D -1.7334 Soluble
75 R D -1.4675 Soluble
76 F D 0.0000 Buried
77 T D -1.0859 Soluble
78 I D 0.0000 Buried
79 S D -0.1180 Soluble
80 A D -0.6028 Soluble
81 D D -1.4213 Soluble
82 T D -1.6521 Soluble
83 S D -1.6008 Soluble
84 K D -2.2628 Soluble
85 N D -1.7253 Soluble
86 T D -0.9397 Soluble
87 A D 0.0000 Buried
88 Y D -0.5342 Soluble
89 L D 0.0000 Buried
90 Q D -1.1421 Soluble
91 M D 0.0000 Buried
92 N D -1.2600 Soluble
93 S D -1.0916 Soluble
94 L D 0.0000 Buried
95 R D -1.8862 Soluble
96 A D -1.5266 Soluble
97 E D -2.1210 Soluble
98 D D 0.0000 Buried
99 T D -0.5376 Soluble
100 A D 0.0000 Buried
101 V D 0.4136 Aggregation-prone
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 S D 0.0000 Buried
106 R D -0.4037 Soluble
107 W D -0.2128 Soluble
108 G D 0.0000 Buried
109 G D -0.8802 Soluble
110 D D -1.7077 Soluble
111 G D 0.0000 Buried
112 F D 1.0706 Aggregation-prone
113 Y D 0.8463 Aggregation-prone
114 A D 0.0000 Buried
115 M D 0.0000 Buried
116 D D -0.1069 Soluble
117 Y D -0.2016 Soluble
118 W D 0.0000 Buried
119 G D 0.0000 Buried
120 Q D -1.1244 Soluble
121 G D -0.3477 Soluble
122 T D 0.3540 Aggregation-prone
123 L D 0.8197 Aggregation-prone
124 V D 0.0000 Buried
125 T D -0.2255 Soluble
126 V D 0.0000 Buried
127 S D -0.8159 Soluble
128 S D -0.6729 Soluble
129 A D -0.4251 Soluble
130 S D -0.5329 Soluble
131 T D -0.7804 Soluble
132 K D -1.1658 Soluble
133 G D -1.4231 Soluble
134 P D 0.0000 Buried
135 S D -0.3408 Soluble
136 V D 0.0000 Buried
137 F D -0.7499 Soluble
138 P D -0.9961 Soluble
139 L D 0.0000 Buried
140 A D -1.2334 Soluble
141 P D 0.0000 Buried
142 S D -1.5083 Soluble
143 S D -1.9511 Soluble
144 K D -2.2364 Soluble
145 S D -1.3038 Soluble
146 T D -0.8132 Soluble
147 S D -0.5637 Soluble
148 G D -0.7804 Soluble
149 G D -1.0082 Soluble
150 T D -0.5506 Soluble
151 A D 0.0000 Buried
152 A D 0.0000 Buried
153 L D 0.0000 Buried
154 G D 0.0000 Buried
155 C D 0.0000 Buried
156 L D 0.0000 Buried
157 V D 0.0000 Buried
158 K D 0.0000 Buried
159 D D -0.3381 Soluble
160 Y D 0.0000 Buried
161 F D 0.0000 Buried
162 P D 0.0000 Buried
163 E D -1.0918 Soluble
164 P D -1.0143 Soluble
165 V D 0.0000 Buried
166 T D -0.6540 Soluble
167 V D -0.3560 Soluble
168 S D -0.3472 Soluble
169 W D 0.0000 Buried
170 N D -0.7912 Soluble
171 S D -0.6782 Soluble
172 G D -0.5859 Soluble
173 A D -0.2625 Soluble
174 L D 0.0055 Aggregation-prone
175 T D -0.1761 Soluble
176 S D -0.1651 Soluble
177 G D -0.2331 Soluble
178 V D 0.2081 Aggregation-prone
179 H D -0.2903 Soluble
180 T D -0.1338 Soluble
181 F D 0.0000 Buried
182 P D -0.2011 Soluble
183 A D 0.2547 Aggregation-prone
184 V D 0.6345 Aggregation-prone
185 L D 1.4581 Aggregation-prone
186 Q D 0.3829 Aggregation-prone
187 S D -0.1287 Soluble
188 S D -0.1723 Soluble
189 G D 0.1059 Aggregation-prone
190 L D 0.2928 Aggregation-prone
191 Y D 0.4291 Aggregation-prone
192 S D 0.0000 Buried
193 L D 0.0000 Buried
194 S D 0.0000 Buried
195 S D 0.0000 Buried
196 V D 0.0000 Buried
197 V D 0.0000 Buried
198 T D -0.1322 Soluble
199 V D 0.0000 Buried
200 P D -0.4989 Soluble
201 S D 0.0000 Buried
202 S D -0.8993 Soluble
203 S D -0.8357 Soluble
204 L D -1.2485 Soluble
205 G D -1.4136 Soluble
206 T D -0.8186 Soluble
207 Q D -1.4309 Soluble
208 T D -1.3463 Soluble
209 Y D 0.0000 Buried
210 I D -1.4253 Soluble
211 C D 0.0000 Buried
212 N D -1.4160 Soluble
213 V D 0.0000 Buried
214 N D -1.7758 Soluble
215 H D 0.0000 Buried
216 K D -2.8374 Soluble
217 P D -1.7306 Soluble
218 S D -1.8339 Soluble
219 N D -2.5886 Soluble
220 T D -2.0608 Soluble
221 K D -2.6165 Soluble
222 V D -1.6703 Soluble
223 D D -2.5574 Soluble
224 K D -2.1106 Soluble
225 K D -2.6340 Soluble
226 V D 0.0000 Buried
227 E D -2.9017 Soluble
228 P D -2.3087 Soluble
229 K D -2.4321 Soluble
230 S D -1.2258 Soluble
231 C D -0.3280 Soluble
1 D C -2.1599 Soluble
2 I C 0.0000 Buried
3 Q C -2.2131 Soluble
4 M C 0.0000 Buried
5 T C -1.1831 Soluble
6 Q C 0.0000 Buried
7 S C -0.6732 Soluble
8 P C -0.6632 Soluble
9 S C -0.9718 Soluble
10 S C -0.9423 Soluble
11 L C -0.6124 Soluble
12 S C -0.5602 Soluble
13 A C -0.8768 Soluble
14 S C -0.8233 Soluble
15 V C -0.1183 Soluble
16 G C -0.9764 Soluble
17 D C -2.1836 Soluble
18 R C -2.5425 Soluble
19 V C 0.0000 Buried
20 T C -0.6793 Soluble
21 I C 0.0000 Buried
22 T C -0.7216 Soluble
23 C C 0.0000 Buried
24 R C -2.4095 Soluble
25 A C 0.0000 Buried
26 S C -2.1780 Soluble
27 Q C -2.9403 Soluble
28 D C -2.9581 Soluble
29 V C 0.0000 Buried
36 N C -2.0353 Soluble
37 T C -0.9648 Soluble
38 A C -0.1172 Soluble
39 V C 0.0000 Buried
40 A C 0.0000 Buried
41 W C 0.0000 Buried
42 Y C 0.0000 Buried
43 Q C 0.0000 Buried
44 Q C 0.0000 Buried
45 K C -2.4468 Soluble
46 P C -1.8221 Soluble
47 G C -1.6492 Soluble
48 K C -2.7103 Soluble
49 A C -1.7381 Soluble
50 P C 0.0000 Buried
51 K C -1.5118 Soluble
52 L C 0.0000 Buried
53 L C 0.0000 Buried
54 I C 0.0000 Buried
55 Y C 1.0055 Aggregation-prone
56 S C 0.5799 Aggregation-prone
57 A C 0.0000 Buried
65 S C 0.2918 Aggregation-prone
66 F C 1.5069 Aggregation-prone
67 L C 0.9105 Aggregation-prone
68 E C 0.0079 Aggregation-prone
69 S C -0.2880 Soluble
70 G C -0.5414 Soluble
71 V C -0.3633 Soluble
72 P C -0.3579 Soluble
74 S C -0.4422 Soluble
75 R C -0.7078 Soluble
76 F C 0.0000 Buried
77 S C 0.0093 Aggregation-prone
78 G C 0.0000 Buried
79 S C -0.8057 Soluble
80 R C -1.7689 Soluble
83 S C -1.3391 Soluble
84 G C -1.6891 Soluble
85 T C -2.1462 Soluble
86 D C -1.8685 Soluble
87 F C 0.0000 Buried
88 T C -0.7476 Soluble
89 L C 0.0000 Buried
90 T C -0.6071 Soluble
91 I C 0.0000 Buried
92 S C -1.4935 Soluble
93 S C -1.2783 Soluble
94 L C 0.0000 Buried
95 Q C -1.1079 Soluble
96 P C -1.4283 Soluble
97 E C -2.2114 Soluble
98 D C 0.0000 Buried
99 F C -1.3511 Soluble
100 A C 0.0000 Buried
101 T C 0.0000 Buried
102 Y C 0.0000 Buried
103 Y C 0.0000 Buried
104 C C 0.0000 Buried
105 Q C 0.0000 Buried
106 Q C 0.0000 Buried
107 H C 0.5241 Aggregation-prone
108 Y C 0.3548 Aggregation-prone
109 T C 0.0120 Aggregation-prone
114 T C -0.5469 Soluble
115 P C -0.9444 Soluble
116 P C 0.0000 Buried
117 T C -0.7316 Soluble
118 F C -0.5022 Soluble
119 G C 0.0000 Buried
120 Q C -1.6608 Soluble
121 G C 0.0000 Buried
122 T C 0.0000 Buried
123 K C -1.9949 Soluble
124 V C 0.0000 Buried
125 E C 0.0000 Buried
126 I C 0.0000 Buried
127 K C -1.1084 Soluble
128 R C -0.8023 Soluble
129 T C 0.0247 Aggregation-prone
130 V C 0.7159 Aggregation-prone
131 A C 0.2307 Aggregation-prone
132 A C -0.0625 Soluble
133 P C 0.0000 Buried
134 S C -0.1168 Soluble
135 V C -0.0172 Soluble
136 F C 0.0000 Buried
137 I C 0.0394 Aggregation-prone
138 F C 0.0000 Buried
139 P C -0.9734 Soluble
140 P C -1.0577 Soluble
141 S C -1.9371 Soluble
142 D C -3.0945 Soluble
143 E C -2.5209 Soluble
144 Q C 0.0000 Buried
145 L C 0.0000 Buried
146 K C -2.7598 Soluble
147 S C -1.6827 Soluble
148 G C -1.5787 Soluble
149 T C -1.3481 Soluble
150 A C 0.0000 Buried
151 S C 0.0000 Buried
152 V C 0.0000 Buried
153 V C 0.0000 Buried
154 C C 0.0000 Buried
155 L C 0.0000 Buried
156 L C 0.0000 Buried
157 N C 0.0000 Buried
158 N C -1.0150 Soluble
159 F C 0.0000 Buried
160 Y C 0.0000 Buried
161 P C -1.2829 Soluble
162 R C -1.8595 Soluble
163 E C -2.7844 Soluble
164 A C -2.0989 Soluble
165 K C -2.2816 Soluble
166 V C -1.0892 Soluble
167 Q C -0.7324 Soluble
168 W C 0.0000 Buried
169 K C -0.6466 Soluble
170 V C 0.0000 Buried
171 D C -2.0862 Soluble
172 N C -1.6391 Soluble
173 A C -0.2948 Soluble
174 L C 0.5883 Aggregation-prone
175 Q C -0.6386 Soluble
176 S C -0.8459 Soluble
177 G C -1.4517 Soluble
178 N C -1.8285 Soluble
179 S C -1.7043 Soluble
180 Q C -1.8886 Soluble
181 E C -1.8820 Soluble
182 S C -0.8561 Soluble
183 V C -0.3123 Soluble
184 T C -0.8455 Soluble
185 E C -1.8200 Soluble
186 Q C 0.0000 Buried
187 D C -1.9738 Soluble
188 S C -2.1555 Soluble
189 K C -2.5810 Soluble
190 D C -1.9448 Soluble
191 S C -1.7751 Soluble
192 T C 0.0000 Buried
193 Y C 0.0000 Buried
194 S C 0.0000 Buried
195 L C 0.0000 Buried
196 S C 0.0000 Buried
197 S C 0.0000 Buried
198 T C -0.8653 Soluble
199 L C 0.0000 Buried
200 T C -0.6802 Soluble
201 L C -0.6939 Soluble
202 S C -1.1551 Soluble
203 K C -2.3728 Soluble
204 A C -2.0637 Soluble
205 D C -2.5866 Soluble
206 Y C 0.0000 Buried
207 E C -4.0114 Soluble
208 K C -3.6896 Soluble
209 H C -3.2656 Soluble
210 K C -3.3494 Soluble
211 V C -1.6873 Soluble
212 Y C 0.0000 Buried
213 A C 0.0000 Buried
214 C C 0.0000 Buried
215 E C -0.8361 Soluble
216 V C 0.0000 Buried
217 T C -1.1843 Soluble
218 H C 0.0000 Buried
219 Q C -1.6054 Soluble
220 G C -0.3567 Soluble
221 L C -0.2548 Soluble
222 S C -0.4557 Soluble
223 S C -0.4099 Soluble
224 P C -0.5674 Soluble
225 V C 0.0693 Aggregation-prone
226 T C -0.4321 Soluble
227 K C -0.7348 Soluble
228 S C -0.6765 Soluble
229 F C -0.9666 Soluble
230 N C -1.9876 Soluble
231 R C -2.3626 Soluble
232 G C -1.9462 Soluble
233 E C -2.4625 Soluble
234 C C -0.5785 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7433 3.5811 CSV PDB
4.5 -0.7893 3.4656 CSV PDB
5.0 -0.8433 3.3358 CSV PDB
5.5 -0.8946 3.2015 CSV PDB
6.0 -0.9312 3.0657 CSV PDB
6.5 -0.9439 2.9283 CSV PDB
7.0 -0.9323 2.7902 CSV PDB
7.5 -0.9045 2.6538 CSV PDB
8.0 -0.8679 2.524 CSV PDB
8.5 -0.8246 2.5192 CSV PDB
9.0 -0.7743 2.5189 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH