Ravagalimab / 6pe9

A4DAB219_6pe9_H_L | Source PDB: 6pe9 | Chains: H, L | Done
98.25
Yes
10 Å
Yes
Avg A4D Score
-0.7177
Max Score
1.2368
Min Score
-3.5245
Total Score
-311.49
Score CDR
-0.3461
Score non-CDR
-0.765

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVKPGGSLRLSCAASGFTFSDYGMNWVRQAPGKGLEWVSYISSGRSNIYYADTVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCARSWGYFDVWGQGTTVTVSSASTKGPSVFPLAPSSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDIVMTQSPDSLAVSLGERATINCKSSQSLLNLGNQKNYLTWFQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQNDYTYPLTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.5245
Maximum Score 1.2368
Average Score -0.7177
Total Score -311.4868

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -1.8741 Soluble
2 V H -0.7626 Soluble
3 Q H -0.8577 Soluble
4 L H 0.0000 Buried
5 V H 0.7876 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.4316 Soluble
8 G H -0.9073 Soluble
9 G H -0.4925 Soluble
11 G H -0.6302 Soluble
12 L H -0.2701 Soluble
13 V H -0.7990 Soluble
14 K H -2.0432 Soluble
15 P H -1.8872 Soluble
16 G H -1.5351 Soluble
17 G H -1.2538 Soluble
18 S H -1.3040 Soluble
19 L H -0.9942 Soluble
20 R H -1.9415 Soluble
21 L H 0.0000 Buried
22 S H -0.3612 Soluble
23 C H 0.0000 Buried
24 A H -0.1285 Soluble
25 A H 0.0000 Buried
26 S H -0.7300 Soluble
27 G H -0.9783 Soluble
28 F H -0.4944 Soluble
29 T H -0.5676 Soluble
30 F H 0.0000 Buried
35 S H -1.1418 Soluble
36 D H -1.2080 Soluble
37 Y H -0.2391 Soluble
38 G H 0.0000 Buried
39 M H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.8677 Soluble
45 A H -1.1780 Soluble
46 P H -1.0110 Soluble
47 G H -1.4713 Soluble
48 K H -2.3342 Soluble
49 G H -1.4781 Soluble
50 L H 0.0000 Buried
51 E H -0.9564 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 Y H 0.6816 Aggregation-prone
56 I H 0.0000 Buried
57 S H -0.4965 Soluble
58 S H -1.1522 Soluble
59 G H -1.7469 Soluble
62 R H -2.1781 Soluble
63 S H -1.1488 Soluble
64 N H -0.5272 Soluble
65 I H 0.8847 Aggregation-prone
66 Y H 0.8698 Aggregation-prone
67 Y H -0.0553 Soluble
68 A H 0.0000 Buried
69 D H -2.3526 Soluble
70 T H -1.6830 Soluble
71 V H 0.0000 Buried
72 K H -2.4405 Soluble
74 G H -1.6657 Soluble
75 R H 0.0000 Buried
76 F H 0.0000 Buried
77 T H -0.5769 Soluble
78 I H 0.0000 Buried
79 S H -0.2420 Soluble
80 R H -0.9850 Soluble
81 D H -1.5205 Soluble
82 N H -1.8564 Soluble
83 A H -1.4292 Soluble
84 K H -2.3400 Soluble
85 N H -1.8519 Soluble
86 S H 0.0000 Buried
87 L H 0.0000 Buried
88 Y H -0.5249 Soluble
89 L H 0.0000 Buried
90 Q H -1.1754 Soluble
91 M H 0.0000 Buried
92 N H -1.4515 Soluble
93 S H -1.2954 Soluble
94 L H 0.0000 Buried
95 R H -2.3052 Soluble
96 A H -1.7557 Soluble
97 E H -2.2068 Soluble
98 D H 0.0000 Buried
99 T H -0.7387 Soluble
100 A H 0.0000 Buried
101 V H -0.0167 Soluble
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 S H 0.3605 Aggregation-prone
108 W H 0.8070 Aggregation-prone
109 G H 0.3311 Aggregation-prone
114 Y H 0.3471 Aggregation-prone
115 F H 0.0000 Buried
116 D H -0.2999 Soluble
117 V H -0.2306 Soluble
118 W H 0.0000 Buried
119 G H 0.0000 Buried
120 Q H -1.2102 Soluble
121 G H -0.5968 Soluble
122 T H -0.2235 Soluble
123 T H -0.2848 Soluble
124 V H 0.0000 Buried
125 T H -0.5860 Soluble
126 V H 0.0000 Buried
127 S H -1.0788 Soluble
128 S H -0.8312 Soluble
129 A H -0.5363 Soluble
130 S H -0.7023 Soluble
131 T H -1.1543 Soluble
132 K H -1.8204 Soluble
133 G H -1.9213 Soluble
134 P H -0.9879 Soluble
135 S H -0.5341 Soluble
136 V H 0.0000 Buried
137 F H -0.5968 Soluble
138 P H -1.1920 Soluble
139 L H 0.0000 Buried
140 A H -1.1962 Soluble
141 P H 0.0000 Buried
142 S H -0.6633 Soluble
143 S H -0.2576 Soluble
144 G H -0.6545 Soluble
145 T H -0.4857 Soluble
146 A H -0.2971 Soluble
147 A H 0.0000 Buried
148 L H 0.0000 Buried
149 G H 0.0000 Buried
150 C H 0.0000 Buried
151 L H 0.0000 Buried
152 V H 0.0000 Buried
153 K H 0.0000 Buried
154 D H -1.0100 Soluble
155 Y H 0.0000 Buried
156 F H -0.5268 Soluble
157 P H 0.0000 Buried
158 E H -0.6270 Soluble
159 P H -0.8835 Soluble
160 V H 0.0000 Buried
161 T H -0.7068 Soluble
162 V H -0.3174 Soluble
163 S H -0.4791 Soluble
164 W H 0.0000 Buried
165 N H -0.8381 Soluble
166 S H -0.7181 Soluble
167 G H -0.5541 Soluble
168 A H -0.2727 Soluble
169 L H -0.0348 Soluble
170 T H -0.2016 Soluble
171 S H -0.1737 Soluble
172 G H -0.1726 Soluble
173 V H 0.1347 Aggregation-prone
174 H H -0.2970 Soluble
175 T H -0.1340 Soluble
176 F H 0.0000 Buried
177 P H -0.2715 Soluble
178 A H 0.1671 Aggregation-prone
179 V H 0.4393 Aggregation-prone
180 L H 1.2368 Aggregation-prone
181 Q H 0.1474 Aggregation-prone
182 S H -0.0872 Soluble
183 S H -0.3191 Soluble
184 G H -0.0598 Soluble
185 L H -0.1262 Soluble
186 Y H 0.2090 Aggregation-prone
187 S H 0.0000 Buried
188 L H 0.0000 Buried
189 S H 0.0000 Buried
190 S H 0.0000 Buried
191 V H 0.0000 Buried
192 V H 0.0000 Buried
193 T H -0.1272 Soluble
194 V H 0.0000 Buried
195 P H -0.5069 Soluble
196 S H -0.5914 Soluble
197 S H -0.6618 Soluble
198 S H -0.7010 Soluble
199 L H -0.8831 Soluble
200 G H -1.0489 Soluble
201 T H -0.8308 Soluble
202 Q H -1.4363 Soluble
203 T H -1.2535 Soluble
204 Y H 0.0000 Buried
205 I H -1.4450 Soluble
206 C H 0.0000 Buried
207 N H -1.5532 Soluble
208 V H 0.0000 Buried
209 N H -2.0956 Soluble
210 H H 0.0000 Buried
211 K H -2.8303 Soluble
212 P H -1.6816 Soluble
213 S H -1.9867 Soluble
214 N H -2.6983 Soluble
215 T H -2.2471 Soluble
216 K H -2.8346 Soluble
217 V H -1.8222 Soluble
218 D H -2.6336 Soluble
219 K H -2.2885 Soluble
220 K H -2.6141 Soluble
221 V H 0.0000 Buried
222 E H -2.9311 Soluble
223 P H -1.9000 Soluble
224 K H -2.3129 Soluble
225 S H -0.8636 Soluble
226 C H 0.0830 Aggregation-prone
1 D L -1.4246 Soluble
2 I L 0.0000 Buried
3 V L 0.7234 Aggregation-prone
4 M L 0.0000 Buried
5 T L -0.8331 Soluble
6 Q L 0.0000 Buried
7 S L -1.3678 Soluble
8 P L -1.2613 Soluble
9 D L -2.2333 Soluble
10 S L -1.2781 Soluble
11 L L -0.7113 Soluble
12 A L -0.5038 Soluble
13 V L 0.0000 Buried
14 S L -1.3698 Soluble
15 L L -0.8727 Soluble
16 G L -1.5547 Soluble
17 E L -2.9255 Soluble
18 R L -2.8980 Soluble
19 A L 0.0000 Buried
20 T L -0.7862 Soluble
21 I L 0.0000 Buried
22 N L -1.4057 Soluble
23 C L 0.0000 Buried
24 K L -2.3128 Soluble
25 S L 0.0000 Buried
26 S L -0.9788 Soluble
27 Q L -1.4725 Soluble
28 S L -0.8449 Soluble
29 L L 0.0000 Buried
30 L L -0.2979 Soluble
31 N L -0.5585 Soluble
32 L L 0.2617 Aggregation-prone
33 G L -0.7136 Soluble
34 N L -1.4632 Soluble
35 Q L -1.5337 Soluble
36 K L -1.2756 Soluble
37 N L -0.4978 Soluble
38 Y L 0.0428 Aggregation-prone
39 L L 0.0000 Buried
40 T L 0.0000 Buried
41 W L 0.0000 Buried
42 F L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -0.8302 Soluble
45 K L -1.1900 Soluble
46 P L -1.0043 Soluble
47 G L -1.1659 Soluble
48 Q L -1.5342 Soluble
49 P L -1.2057 Soluble
50 P L 0.0000 Buried
51 K L -1.4670 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.0079 Aggregation-prone
56 W L 0.2206 Aggregation-prone
57 A L 0.0000 Buried
65 S L -0.4751 Soluble
66 T L -0.3597 Soluble
67 R L -1.2710 Soluble
68 E L -1.2787 Soluble
69 S L -0.7849 Soluble
70 G L -1.0355 Soluble
71 V L 0.0000 Buried
72 P L -1.3858 Soluble
74 D L -2.2483 Soluble
75 R L -1.7179 Soluble
76 F L 0.0000 Buried
77 S L -0.8681 Soluble
78 G L -0.4698 Soluble
79 S L -0.6757 Soluble
80 G L -1.2504 Soluble
83 S L -1.0340 Soluble
84 G L -1.1472 Soluble
85 T L -1.6549 Soluble
86 D L -2.2595 Soluble
87 F L 0.0000 Buried
88 T L -0.9357 Soluble
89 L L 0.0000 Buried
90 T L -0.6688 Soluble
91 I L 0.0000 Buried
92 S L -1.9500 Soluble
93 S L -1.7375 Soluble
94 L L 0.0000 Buried
95 Q L -1.6590 Soluble
96 A L -1.6769 Soluble
97 E L -1.7516 Soluble
98 D L 0.0000 Buried
99 V L 0.0000 Buried
100 A L 0.0000 Buried
101 V L -0.4466 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 N L 0.0000 Buried
107 D L 0.3644 Aggregation-prone
108 Y L 0.4374 Aggregation-prone
109 T L 0.2579 Aggregation-prone
114 Y L 0.4634 Aggregation-prone
115 P L -0.2478 Soluble
116 L L 0.0000 Buried
117 T L 0.0510 Aggregation-prone
118 F L 0.0792 Aggregation-prone
119 G L 0.0000 Buried
120 Q L -1.2371 Soluble
121 G L -1.1902 Soluble
122 T L 0.0000 Buried
123 K L -1.1700 Soluble
124 L L 0.0000 Buried
125 E L -1.1423 Soluble
126 I L 0.0000 Buried
127 K L -1.3820 Soluble
128 R L -0.7047 Soluble
129 T L 0.0931 Aggregation-prone
130 V L 0.6335 Aggregation-prone
131 A L 0.0917 Aggregation-prone
132 A L -0.0697 Soluble
133 P L 0.0000 Buried
134 S L -0.1461 Soluble
135 V L 0.0000 Buried
136 F L 0.0105 Aggregation-prone
137 I L 0.0150 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.5091 Soluble
140 P L 0.0000 Buried
141 S L -1.6944 Soluble
142 D L -2.9763 Soluble
143 E L -2.5126 Soluble
144 Q L 0.0000 Buried
145 L L -2.1379 Soluble
146 K L -2.6921 Soluble
147 S L -1.6843 Soluble
148 G L -1.2251 Soluble
149 T L -0.9660 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.8823 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.5890 Soluble
162 R L -2.7767 Soluble
163 E L -3.1453 Soluble
164 A L -2.3712 Soluble
165 K L -2.5080 Soluble
166 V L -1.4286 Soluble
167 Q L -0.7771 Soluble
168 W L 0.0000 Buried
169 K L -0.6534 Soluble
170 V L 0.0000 Buried
171 D L -1.9421 Soluble
172 N L -1.5620 Soluble
173 A L -0.2816 Soluble
174 L L 0.6888 Aggregation-prone
175 Q L -0.2861 Soluble
176 S L -0.6386 Soluble
177 G L -1.3081 Soluble
178 N L -1.5778 Soluble
179 S L -1.7229 Soluble
180 Q L -2.0688 Soluble
181 E L -2.2457 Soluble
182 S L -0.9956 Soluble
183 V L -0.6495 Soluble
184 T L -0.8178 Soluble
185 E L -1.5750 Soluble
186 Q L 0.0000 Buried
187 D L -2.0899 Soluble
188 S L -2.1480 Soluble
189 K L -2.6116 Soluble
190 D L -1.8807 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.9339 Soluble
199 L L 0.0000 Buried
200 T L -0.6998 Soluble
201 L L -0.7869 Soluble
202 S L -1.0363 Soluble
203 K L -1.9691 Soluble
204 A L -1.8334 Soluble
205 D L -2.5802 Soluble
206 Y L 0.0000 Buried
207 E L -3.3279 Soluble
208 K L -3.5245 Soluble
209 H L -3.0262 Soluble
210 K L -3.0578 Soluble
211 V L -1.4064 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -1.0066 Soluble
216 V L 0.0000 Buried
217 T L -1.2234 Soluble
218 H L 0.0000 Buried
219 Q L -1.6459 Soluble
220 G L -0.3508 Soluble
221 L L -0.2095 Soluble
222 S L -0.4781 Soluble
223 S L -0.3922 Soluble
224 P L -0.5850 Soluble
225 V L 0.0032 Aggregation-prone
226 T L -0.5692 Soluble
227 K L -0.8707 Soluble
228 S L -0.6604 Soluble
229 F L -0.8180 Soluble
230 N L -1.8057 Soluble
231 R L -2.4436 Soluble
232 G L -2.0811 Soluble
233 E L -2.0610 Soluble
234 C L -0.5185 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6869 3.4678 CSV PDB
4.5 -0.7353 3.4183 CSV PDB
5.0 -0.7906 3.3622 CSV PDB
5.5 -0.8421 3.3034 CSV PDB
6.0 -0.878 3.2438 CSV PDB
6.5 -0.8891 3.1841 CSV PDB
7.0 -0.8757 3.1248 CSV PDB
7.5 -0.8462 3.067 CSV PDB
8.0 -0.8076 3.013 CSV PDB
8.5 -0.7615 2.9671 CSV PDB
9.0 -0.7078 2.9336 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH