Gantenerumab / 5csz

A4DAB124_5csz_H_L | Source PDB: 5csz | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6995
Max Score
1.2186
Min Score
-3.7182
Total Score
-297.97
Score CDR
—
Score non-CDR
—

Calculation Parameters

Chain Sequence(s) VELVESGGGLVQPGGSLRLSCAASGFTFSSYAMSWVRQAPGKGLEWVSAINASGTRTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARGKGYVRYFDVWGQGTLVTVSSASTKGPSVFPLAPSTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSDIVLTQSPATLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGVPARFSGSGSGTDFTLTISSLEPEDFATYYCLQIYNMPITFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.7182
Maximum Score 1.2186
Average Score -0.6995
Total Score -297.9709

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V H 0.7429 Aggregation-prone
3 E H -0.9356 Soluble
4 L H 0.0000 Buried
5 V H 0.1980 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.5105 Soluble
8 G H -0.8158 Soluble
9 G H -0.4431 Soluble
11 G H -0.2500 Soluble
12 L H -0.0312 Soluble
13 V H -0.6152 Soluble
14 Q H -1.5914 Soluble
15 P H -1.6857 Soluble
16 G H -1.3595 Soluble
17 G H 0.0000 Buried
18 S H -1.1794 Soluble
19 L H -0.9670 Soluble
20 R H -2.0076 Soluble
21 L H 0.0000 Buried
22 S H -0.5035 Soluble
23 C H 0.0000 Buried
24 A H -0.4520 Soluble
25 A H 0.0000 Buried
26 S H -0.4200 Soluble
27 G H -0.1473 Soluble
28 F H 0.0719 Aggregation-prone
29 T H -0.0612 Soluble
30 F H 0.0000 Buried
35 S H -0.7877 Soluble
36 S H -0.2447 Soluble
37 Y H -0.0737 Soluble
38 A H -0.2725 Soluble
39 M H 0.0000 Buried
40 S H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.8145 Soluble
45 A H -1.1811 Soluble
46 P H -0.9383 Soluble
47 G H -1.4954 Soluble
48 K H -2.3960 Soluble
49 G H -1.6763 Soluble
50 L H 0.0000 Buried
51 E H -1.1798 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 A H 0.0000 Buried
56 I H 0.0000 Buried
57 N H -0.8368 Soluble
58 A H -0.6537 Soluble
59 S H -0.9219 Soluble
62 G H -1.1605 Soluble
63 T H -1.2256 Soluble
64 R H -2.0315 Soluble
65 T H -0.9314 Soluble
66 Y H -0.5315 Soluble
67 Y H -0.7590 Soluble
68 A H 0.0000 Buried
69 D H -2.3959 Soluble
70 S H -1.7429 Soluble
71 V H 0.0000 Buried
72 K H -2.5134 Soluble
74 G H -1.6832 Soluble
75 R H -1.3763 Soluble
76 F H 0.0000 Buried
77 T H -0.8738 Soluble
78 I H 0.0000 Buried
79 S H -0.7179 Soluble
80 R H -1.0448 Soluble
81 D H -1.5767 Soluble
82 N H -1.6986 Soluble
83 S H -1.5764 Soluble
84 K H -2.3881 Soluble
85 N H -1.7644 Soluble
86 T H -1.0800 Soluble
87 L H 0.0000 Buried
88 Y H -0.6345 Soluble
89 L H 0.0000 Buried
90 Q H -1.4416 Soluble
91 M H 0.0000 Buried
92 N H -1.2925 Soluble
93 S H -1.1674 Soluble
94 L H 0.0000 Buried
95 R H -2.3205 Soluble
96 A H -1.7372 Soluble
97 E H -2.2418 Soluble
98 D H 0.0000 Buried
99 T H -0.7207 Soluble
100 A H 0.0000 Buried
101 V H 0.2895 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 G H 0.0000 Buried
108 K H -0.5965 Soluble
109 G H -0.4006 Soluble
110 Y H 1.2186 Aggregation-prone
112 V H 0.4711 Aggregation-prone
113 R H -0.0571 Soluble
114 Y H 0.0000 Buried
115 F H 0.0000 Buried
116 D H -0.5846 Soluble
117 V H -0.1391 Soluble
118 W H -0.2670 Soluble
119 G H 0.0000 Buried
120 Q H -1.4240 Soluble
121 G H 0.0000 Buried
122 T H -0.0163 Soluble
123 L H 0.3545 Aggregation-prone
124 V H 0.0000 Buried
125 T H -0.4317 Soluble
126 V H 0.0000 Buried
127 S H -1.0093 Soluble
128 S H -0.7388 Soluble
129 A H -0.4786 Soluble
130 S H -0.5864 Soluble
131 T H -0.5802 Soluble
132 K H -1.1292 Soluble
133 G H -1.3748 Soluble
134 P H 0.0000 Buried
135 S H -0.2474 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.3317 Soluble
139 L H 0.0000 Buried
140 A H -1.2484 Soluble
141 P H 0.0000 Buried
142 S H -0.6723 Soluble
143 T H -0.2598 Soluble
144 A H -0.1671 Soluble
145 A H 0.0000 Buried
146 L H 0.0000 Buried
147 G H 0.0000 Buried
148 C H 0.0000 Buried
149 L H 0.0000 Buried
150 V H 0.0000 Buried
151 K H 0.0000 Buried
152 D H -0.4792 Soluble
153 Y H 0.0000 Buried
154 F H 0.0000 Buried
155 P H 0.0000 Buried
156 E H -0.5556 Soluble
157 P H -0.7954 Soluble
158 V H -0.8390 Soluble
159 T H -0.7132 Soluble
160 V H -0.3959 Soluble
161 S H -0.4442 Soluble
162 W H 0.0000 Buried
163 N H -0.7791 Soluble
164 S H -0.6844 Soluble
165 G H -0.5372 Soluble
166 A H -0.2568 Soluble
167 L H -0.0282 Soluble
168 T H -0.1783 Soluble
169 S H -0.3708 Soluble
170 G H -0.4464 Soluble
171 V H 0.1356 Aggregation-prone
172 H H 0.0000 Buried
173 T H 0.0554 Aggregation-prone
174 F H 0.0000 Buried
175 P H -0.3563 Soluble
176 A H 0.1398 Aggregation-prone
177 V H 0.4476 Aggregation-prone
178 L H 0.9762 Aggregation-prone
179 Q H 0.1538 Aggregation-prone
180 S H -0.1789 Soluble
181 S H -0.2331 Soluble
182 G H -0.0074 Soluble
183 L H 0.0746 Aggregation-prone
184 Y H 0.3754 Aggregation-prone
185 S H 0.0000 Buried
186 L H 0.0000 Buried
187 S H 0.0000 Buried
188 S H 0.0000 Buried
189 V H 0.0000 Buried
190 V H 0.0000 Buried
191 T H -0.1493 Soluble
192 V H 0.0000 Buried
193 P H -0.4993 Soluble
194 S H -0.4682 Soluble
195 S H -0.5237 Soluble
196 S H -0.5631 Soluble
197 L H -0.7721 Soluble
198 G H -1.2942 Soluble
199 T H -0.8504 Soluble
200 Q H -1.1345 Soluble
201 T H -1.1173 Soluble
202 Y H 0.0000 Buried
203 I H -1.3006 Soluble
204 C H 0.0000 Buried
205 N H -1.4827 Soluble
206 V H 0.0000 Buried
207 N H -2.3045 Soluble
208 H H 0.0000 Buried
209 K H -2.8541 Soluble
210 P H -1.6377 Soluble
211 S H -1.8491 Soluble
212 N H -2.6604 Soluble
213 T H -2.1504 Soluble
214 K H -2.7861 Soluble
215 V H -1.5788 Soluble
216 D H -2.4118 Soluble
217 K H -2.0629 Soluble
218 K H -2.4890 Soluble
219 V H 0.0000 Buried
220 E H -2.9431 Soluble
221 P H -1.9898 Soluble
222 K H -2.6282 Soluble
223 S H -1.3302 Soluble
1 D L -1.5446 Soluble
2 I L -0.3534 Soluble
3 V L 0.8599 Aggregation-prone
4 L L 0.0000 Buried
5 T L -0.3843 Soluble
6 Q L 0.0000 Buried
7 S L -0.5710 Soluble
8 P L -0.2924 Soluble
9 A L -0.5338 Soluble
10 T L -0.5774 Soluble
11 L L -0.3822 Soluble
12 S L -0.6017 Soluble
13 L L -0.9639 Soluble
14 S L -1.3973 Soluble
15 P L -1.7730 Soluble
16 G L -1.8564 Soluble
17 E L -2.4718 Soluble
18 R L -2.6241 Soluble
19 A L 0.0000 Buried
20 T L -0.6757 Soluble
21 L L 0.0000 Buried
22 S L -0.7667 Soluble
23 C L 0.0000 Buried
24 R L -1.7123 Soluble
25 A L 0.0000 Buried
26 S L -0.8314 Soluble
27 Q L -1.6259 Soluble
28 S L -1.2075 Soluble
29 V L 0.0000 Buried
30 S L -0.1936 Soluble
36 S L -0.4173 Soluble
37 S L -0.0672 Soluble
38 Y L 0.2714 Aggregation-prone
39 L L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.6723 Soluble
46 P L -1.4630 Soluble
47 G L -1.4725 Soluble
48 Q L -2.0766 Soluble
49 A L -1.3008 Soluble
50 P L 0.0000 Buried
51 R L -1.1378 Soluble
52 L L -0.5035 Soluble
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.2452 Soluble
56 G L -0.3629 Soluble
57 A L 0.0000 Buried
65 S L -0.6491 Soluble
66 S L -0.7060 Soluble
67 R L -1.2519 Soluble
68 A L -0.7958 Soluble
69 T L -0.5696 Soluble
70 G L -0.6693 Soluble
71 V L 0.0000 Buried
72 P L -0.4647 Soluble
74 A L -0.4067 Soluble
75 R L -0.8300 Soluble
76 F L 0.0000 Buried
77 S L -0.5639 Soluble
78 G L 0.0000 Buried
79 S L -0.7721 Soluble
80 G L -1.2101 Soluble
83 S L -1.2269 Soluble
84 G L -1.3342 Soluble
85 T L -1.8508 Soluble
86 D L -2.6069 Soluble
87 F L 0.0000 Buried
88 T L -0.8135 Soluble
89 L L 0.0000 Buried
90 T L -0.6081 Soluble
91 I L 0.0000 Buried
92 S L -1.4070 Soluble
93 S L -1.7760 Soluble
94 L L 0.0000 Buried
95 E L -2.0224 Soluble
96 P L -1.8718 Soluble
97 E L -2.3119 Soluble
98 D L 0.0000 Buried
99 F L -1.0263 Soluble
100 A L 0.0000 Buried
101 T L -0.9253 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 L L 0.0000 Buried
106 Q L 0.0000 Buried
107 I L 0.0000 Buried
108 Y L 0.4508 Aggregation-prone
109 N L -0.9576 Soluble
114 M L -0.4244 Soluble
115 P L -0.5174 Soluble
116 I L 0.0000 Buried
117 T L -0.1140 Soluble
118 F L 0.0028 Aggregation-prone
119 G L 0.0000 Buried
120 Q L -1.5204 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -1.6138 Soluble
124 V L 0.0000 Buried
125 E L -1.0837 Soluble
126 I L -0.7446 Soluble
127 K L -1.0993 Soluble
128 R L -0.8443 Soluble
129 T L -0.0230 Soluble
130 V L 0.4157 Aggregation-prone
131 A L 0.0058 Aggregation-prone
132 A L -0.1209 Soluble
133 P L 0.0000 Buried
134 S L -0.1288 Soluble
135 V L 0.0000 Buried
136 F L 0.1085 Aggregation-prone
137 I L 0.0799 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.6132 Soluble
140 P L 0.0000 Buried
141 S L -1.9352 Soluble
142 D L -3.2380 Soluble
143 E L -3.2024 Soluble
144 Q L 0.0000 Buried
145 L L -2.4092 Soluble
146 K L -2.8973 Soluble
147 S L -1.7787 Soluble
148 G L -1.3463 Soluble
149 T L -0.9715 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.7563 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.4992 Soluble
162 R L -2.6921 Soluble
163 E L -3.0605 Soluble
164 A L -2.3102 Soluble
165 K L -2.5549 Soluble
166 V L 0.0000 Buried
167 Q L -0.8491 Soluble
168 W L 0.0000 Buried
169 K L -0.8411 Soluble
170 V L 0.0000 Buried
171 D L -2.1708 Soluble
172 N L -1.6711 Soluble
173 A L -0.3583 Soluble
174 L L 0.6004 Aggregation-prone
175 Q L -0.1425 Soluble
176 S L -0.3753 Soluble
177 G L -0.7189 Soluble
178 N L -0.8265 Soluble
179 S L -1.0427 Soluble
180 Q L -1.2237 Soluble
181 E L -1.7489 Soluble
182 S L -0.7843 Soluble
183 V L -0.8528 Soluble
184 T L -1.1501 Soluble
185 E L -2.2495 Soluble
186 Q L -1.8143 Soluble
187 D L -1.7881 Soluble
188 S L -1.8887 Soluble
189 K L -2.2505 Soluble
190 D L -1.5810 Soluble
191 S L -1.6186 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.5400 Soluble
199 L L 0.0000 Buried
200 T L -0.3168 Soluble
201 L L -0.6022 Soluble
202 S L -1.1528 Soluble
203 K L -1.9819 Soluble
204 A L -1.8506 Soluble
205 D L -2.4655 Soluble
206 Y L 0.0000 Buried
207 E L -3.6787 Soluble
208 K L -3.7182 Soluble
209 H L -3.3174 Soluble
210 K L -3.3603 Soluble
211 V L -1.6730 Soluble
212 Y L 0.0000 Buried
213 A L -1.0155 Soluble
214 C L 0.0000 Buried
215 E L -1.2100 Soluble
216 V L 0.0000 Buried
217 T L -1.3392 Soluble
218 H L 0.0000 Buried
219 Q L -1.7393 Soluble
220 G L -0.4353 Soluble
221 L L -0.2461 Soluble
222 S L -0.4757 Soluble
223 S L -0.4207 Soluble
224 P L -0.5960 Soluble
225 V L -0.0586 Soluble
226 T L -0.6002 Soluble
227 K L -0.7134 Soluble
228 S L -0.7613 Soluble
229 F L -0.9779 Soluble
230 N L -2.1449 Soluble
231 R L -2.4660 Soluble
232 G L -1.5514 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7376 1.5767 CSV PDB
4.5 -0.7799 1.5217 CSV PDB
5.0 -0.8302 1.4367 CSV PDB
5.5 -0.879 1.3353 CSV PDB
6.0 -0.9149 1.2337 CSV PDB
6.5 -0.9286 1.2428 CSV PDB
7.0 -0.919 1.2674 CSV PDB
7.5 -0.8932 1.2936 CSV PDB
8.0 -0.858 1.3784 CSV PDB
8.5 -0.8164 1.5332 CSV PDB
9.0 -0.7688 1.6885 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH