Izalontamab / 5id0

A4DAB149_5id0_D_C | Source PDB: 5id0 | Chains: D, C | Done
99.56
Yes
10 Å
Yes
Avg A4D Score
-0.657
Max Score
2.008
Min Score
-3.64
Total Score
-277.24
Score CDR
-0.247
Score non-CDR
-0.7071

Calculation Parameters

Chain Sequence(s) VQLKQSGPGLVQPSQSLSITCTVSGFSLTNYGVHWVRQSPGKGLEWLGVIWSGGNTDYNTPFTSRLSINKDNSKSQVFFKMNSLQSNDTAIYYCARALTYYDYEFAYWGQGTLVTVSAASTKGPSVFPLAGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKDILLTQSPVILSVSPGERVSFSCRASQSIGTNIHWYQQRTNGSPRLLIKYASESISGIPSRFSGSGSGTDFTLSINSVESEDIADYYCQQNNNWPTTFGAGTKLELKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG input.pdb
Selected Chain(s) D (Heavy), C (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.64
Maximum Score 2.008
Average Score -0.657
Total Score -277.2364

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V D 0.4313 Aggregation-prone
3 Q D -1.0856 Soluble
4 L D 0.0000 Buried
5 K D -2.3867 Soluble
6 Q D 0.0000 Buried
7 S D -0.8849 Soluble
8 G D -1.1571 Soluble
9 P D -0.4932 Soluble
11 G D -0.4136 Soluble
12 L D -0.0947 Soluble
13 V D -0.7885 Soluble
14 Q D -1.5701 Soluble
15 P D -1.6912 Soluble
16 S D -1.6667 Soluble
17 Q D -2.0553 Soluble
18 S D -1.8239 Soluble
19 L D 0.0000 Buried
20 S D -0.6032 Soluble
21 I D 0.0000 Buried
22 T D -0.5305 Soluble
23 C D 0.0000 Buried
24 T D -1.2018 Soluble
25 V D 0.0000 Buried
26 S D -0.8442 Soluble
27 G D -0.2750 Soluble
28 F D -0.1788 Soluble
29 S D -0.7990 Soluble
30 L D 0.0000 Buried
35 T D -0.9236 Soluble
36 N D -0.8521 Soluble
37 Y D 0.4818 Aggregation-prone
38 G D 0.0000 Buried
39 V D 0.0000 Buried
40 H D 0.0000 Buried
41 W D 0.0000 Buried
42 V D 0.0000 Buried
43 R D 0.0000 Buried
44 Q D -0.4645 Soluble
45 S D -0.9963 Soluble
46 P D -1.1409 Soluble
47 G D -1.7218 Soluble
48 K D -2.2687 Soluble
49 G D -1.1816 Soluble
50 L D 0.0000 Buried
51 E D -0.5997 Soluble
52 W D 0.0000 Buried
53 L D 0.0000 Buried
54 G D 0.0000 Buried
55 V D 0.0000 Buried
56 I D 0.0000 Buried
57 W D -0.5062 Soluble
58 S D -0.6433 Soluble
59 G D -1.2211 Soluble
63 G D -1.5283 Soluble
64 N D -1.8351 Soluble
65 T D -1.2780 Soluble
66 D D -0.9860 Soluble
67 Y D -0.3945 Soluble
68 N D -0.2342 Soluble
69 T D -0.3489 Soluble
70 P D -0.4005 Soluble
71 F D 0.0000 Buried
72 T D -0.4792 Soluble
74 S D -0.6698 Soluble
75 R D -1.1823 Soluble
76 L D 0.0000 Buried
77 S D -0.9989 Soluble
78 I D 0.0000 Buried
79 N D -1.7153 Soluble
80 K D -1.4973 Soluble
81 D D -1.8597 Soluble
82 N D -2.2746 Soluble
83 S D -1.7260 Soluble
84 K D -2.3949 Soluble
85 S D -1.6281 Soluble
86 Q D -1.5699 Soluble
87 V D 0.0000 Buried
88 F D -0.2998 Soluble
89 F D 0.0000 Buried
90 K D -1.3444 Soluble
91 M D 0.0000 Buried
92 N D -2.2604 Soluble
93 S D -1.8089 Soluble
94 L D 0.0000 Buried
95 Q D -2.1690 Soluble
96 S D -1.5195 Soluble
97 N D -2.0510 Soluble
98 D D 0.0000 Buried
99 T D -0.5933 Soluble
100 A D 0.0000 Buried
101 I D 0.5783 Aggregation-prone
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 A D 0.0000 Buried
106 R D 0.0000 Buried
107 A D 0.0000 Buried
108 L D 1.1905 Aggregation-prone
109 T D 1.2525 Aggregation-prone
110 Y D 1.9126 Aggregation-prone
111 Y D 2.0080 Aggregation-prone
112 D D 0.0000 Buried
113 Y D 0.7199 Aggregation-prone
114 E D 0.0000 Buried
115 F D 0.0000 Buried
116 A D 0.4137 Aggregation-prone
117 Y D 0.3745 Aggregation-prone
118 W D 0.0000 Buried
119 G D 0.0000 Buried
120 Q D -1.8549 Soluble
121 G D -0.9104 Soluble
122 T D 0.0000 Buried
123 L D 0.2221 Aggregation-prone
124 V D 0.0000 Buried
125 T D -0.3295 Soluble
126 V D 0.0000 Buried
127 S D -0.6663 Soluble
128 A D -0.3479 Soluble
129 A D -0.3456 Soluble
130 S D -0.6654 Soluble
131 T D -1.0150 Soluble
132 K D -1.8989 Soluble
133 G D -1.8050 Soluble
134 P D -0.8283 Soluble
135 S D -0.4088 Soluble
136 V D 0.0000 Buried
137 F D -0.4402 Soluble
138 P D 0.0000 Buried
139 L D 0.0000 Buried
140 A D -0.8910 Soluble
141 G D -0.6434 Soluble
142 T D -0.4777 Soluble
143 A D -0.2205 Soluble
144 A D 0.0318 Aggregation-prone
145 L D 0.0000 Buried
146 G D 0.0000 Buried
147 C D 0.0000 Buried
148 L D 0.0000 Buried
149 V D 0.0000 Buried
150 K D 0.0000 Buried
151 D D -0.6465 Soluble
152 Y D 0.0000 Buried
153 F D 0.0000 Buried
154 P D 0.0000 Buried
155 E D -0.4081 Soluble
156 P D -0.5681 Soluble
157 V D -0.6066 Soluble
158 T D -0.5192 Soluble
159 V D -0.2170 Soluble
160 S D -0.3849 Soluble
161 W D 0.0000 Buried
162 N D -0.7285 Soluble
163 S D -0.6456 Soluble
164 G D -0.5009 Soluble
165 A D -0.2171 Soluble
166 L D 0.0164 Aggregation-prone
167 T D -0.1603 Soluble
168 S D -0.1823 Soluble
169 G D -0.2298 Soluble
170 V D 0.1423 Aggregation-prone
171 H D -0.3043 Soluble
172 T D -0.0046 Soluble
173 F D 0.0000 Buried
174 P D -0.3090 Soluble
175 A D 0.2752 Aggregation-prone
176 V D 0.5716 Aggregation-prone
177 L D 1.3033 Aggregation-prone
178 Q D 0.3522 Aggregation-prone
179 S D -0.0395 Soluble
180 S D -0.1849 Soluble
181 G D 0.1092 Aggregation-prone
182 L D 0.1183 Aggregation-prone
183 Y D 0.3930 Aggregation-prone
184 S D 0.0000 Buried
185 L D 0.0000 Buried
186 S D 0.0000 Buried
187 S D 0.0000 Buried
188 V D 0.0000 Buried
189 V D 0.0000 Buried
190 T D -0.1322 Soluble
191 V D 0.0000 Buried
192 P D -0.4828 Soluble
193 S D -0.5185 Soluble
194 S D -0.6014 Soluble
195 S D -0.5847 Soluble
196 L D -0.6667 Soluble
197 G D -0.8741 Soluble
198 T D -0.7175 Soluble
199 Q D -1.1229 Soluble
200 T D -1.0306 Soluble
201 Y D 0.0000 Buried
202 I D -1.2676 Soluble
203 C D 0.0000 Buried
204 N D -1.4399 Soluble
205 V D 0.0000 Buried
206 N D -1.8821 Soluble
207 H D 0.0000 Buried
208 K D -2.5886 Soluble
209 P D -1.6157 Soluble
210 S D -1.9286 Soluble
211 N D -2.5976 Soluble
212 T D -2.1095 Soluble
213 K D -2.6299 Soluble
214 V D -1.5038 Soluble
215 D D -2.4224 Soluble
216 K D -2.0363 Soluble
217 R D -2.4038 Soluble
218 V D 0.0000 Buried
219 E D -2.0610 Soluble
220 P D -1.5328 Soluble
221 K D -2.2286 Soluble
1 D C -1.2665 Soluble
2 I C -0.2954 Soluble
3 L C 0.7070 Aggregation-prone
4 L C 0.0000 Buried
5 T C -0.4272 Soluble
6 Q C -0.3701 Soluble
7 S C 0.0394 Aggregation-prone
8 P C 0.7871 Aggregation-prone
9 V C 1.8526 Aggregation-prone
10 I C 1.3364 Aggregation-prone
11 L C 0.5516 Aggregation-prone
12 S C -0.1719 Soluble
13 V C -1.0306 Soluble
14 S C -1.7484 Soluble
15 P C -1.9810 Soluble
16 G C -2.1498 Soluble
17 E C -3.0623 Soluble
18 R C -3.3234 Soluble
19 V C 0.0000 Buried
20 S C -0.9866 Soluble
21 F C 0.0000 Buried
22 S C -0.9020 Soluble
23 C C 0.0000 Buried
24 R C -2.4975 Soluble
25 A C 0.0000 Buried
26 S C -0.9003 Soluble
27 Q C -1.2394 Soluble
28 S C -1.4418 Soluble
29 I C 0.0000 Buried
36 G C -0.9725 Soluble
37 T C -0.7398 Soluble
38 N C -0.7643 Soluble
39 I C 0.0000 Buried
40 H C 0.0000 Buried
41 W C 0.0000 Buried
42 Y C 0.0000 Buried
43 Q C 0.0000 Buried
44 Q C -1.2869 Soluble
45 R C -1.9700 Soluble
46 T C -1.7395 Soluble
47 N C -1.8126 Soluble
48 G C -1.5375 Soluble
49 S C -0.9891 Soluble
50 P C 0.0000 Buried
51 R C -0.6872 Soluble
52 L C 0.0000 Buried
53 L C 0.0000 Buried
54 I C 0.0000 Buried
55 K C -0.7334 Soluble
56 Y C -0.5692 Soluble
57 A C 0.0000 Buried
65 S C -1.2438 Soluble
66 E C -1.9895 Soluble
67 S C -1.1825 Soluble
68 I C -0.4922 Soluble
69 S C -0.3444 Soluble
70 G C -0.4706 Soluble
71 I C -0.2538 Soluble
72 P C -0.3134 Soluble
74 S C -0.5674 Soluble
75 R C -1.1052 Soluble
76 F C 0.0000 Buried
77 S C -0.9356 Soluble
78 G C -0.7663 Soluble
79 S C -0.8550 Soluble
80 G C -1.0879 Soluble
83 S C -0.9176 Soluble
84 G C -1.2632 Soluble
85 T C -1.8429 Soluble
86 D C -2.5028 Soluble
87 F C 0.0000 Buried
88 T C -0.9058 Soluble
89 L C 0.0000 Buried
90 S C -0.9592 Soluble
91 I C 0.0000 Buried
92 N C -2.4847 Soluble
93 S C -2.3431 Soluble
94 V C 0.0000 Buried
95 E C -2.7071 Soluble
96 S C -2.3788 Soluble
97 E C -2.3227 Soluble
98 D C 0.0000 Buried
99 I C 0.0000 Buried
100 A C 0.0000 Buried
101 D C -0.8346 Soluble
102 Y C 0.0000 Buried
103 Y C 0.0000 Buried
104 C C 0.0000 Buried
105 Q C 0.0000 Buried
106 Q C 0.0000 Buried
107 N C -0.5580 Soluble
108 N C -1.1515 Soluble
109 N C -1.0047 Soluble
114 W C 0.0350 Aggregation-prone
115 P C 0.0000 Buried
116 T C 0.0000 Buried
117 T C -0.0237 Soluble
118 F C 0.1921 Aggregation-prone
119 G C 0.0000 Buried
120 A C 0.0146 Aggregation-prone
121 G C 0.0765 Aggregation-prone
122 T C 0.0000 Buried
123 K C -0.1994 Soluble
124 L C 0.0000 Buried
125 E C -1.1098 Soluble
126 L C 0.0000 Buried
127 K C -1.4864 Soluble
128 R C -1.0828 Soluble
129 T C 0.1840 Aggregation-prone
130 V C 0.9986 Aggregation-prone
131 A C 0.4707 Aggregation-prone
132 A C 0.0703 Aggregation-prone
133 P C 0.0000 Buried
134 S C -0.1650 Soluble
135 V C 0.0000 Buried
136 F C 0.1859 Aggregation-prone
137 I C 0.1573 Aggregation-prone
138 F C 0.0000 Buried
139 P C -0.5989 Soluble
140 P C -0.6421 Soluble
141 S C 0.0000 Buried
142 D C -2.5658 Soluble
143 E C -1.9334 Soluble
144 Q C 0.0000 Buried
145 L C -1.9620 Soluble
146 K C -2.4665 Soluble
147 S C -1.5460 Soluble
148 G C -1.1478 Soluble
149 T C -0.9141 Soluble
150 A C 0.0000 Buried
151 S C 0.0000 Buried
152 V C 0.0000 Buried
153 V C 0.0000 Buried
154 C C 0.0000 Buried
155 L C 0.0000 Buried
156 L C 0.0000 Buried
157 N C 0.0000 Buried
158 N C -0.9096 Soluble
159 F C 0.0000 Buried
160 Y C 0.0000 Buried
161 P C -1.5717 Soluble
162 R C -2.8777 Soluble
163 E C -2.8658 Soluble
164 A C -1.4822 Soluble
166 V C -0.6569 Soluble
167 Q C -1.0740 Soluble
168 W C 0.0000 Buried
169 K C -0.9486 Soluble
170 V C 0.0000 Buried
171 D C -2.1107 Soluble
172 N C -1.6415 Soluble
173 A C -0.4428 Soluble
174 L C 0.1859 Aggregation-prone
175 Q C -0.4218 Soluble
176 S C -0.5722 Soluble
177 G C -0.8723 Soluble
178 N C -0.7933 Soluble
179 S C -1.1257 Soluble
180 Q C -1.1822 Soluble
181 E C -1.5142 Soluble
182 S C -0.8257 Soluble
183 V C -0.9467 Soluble
184 T C -1.1532 Soluble
185 E C -2.3016 Soluble
186 Q C 0.0000 Buried
187 D C -2.0097 Soluble
188 S C -2.4064 Soluble
189 K C -2.7105 Soluble
190 D C -1.9464 Soluble
191 S C 0.0000 Buried
192 T C 0.0000 Buried
193 Y C -1.4071 Soluble
194 S C 0.0000 Buried
195 L C 0.0000 Buried
196 S C 0.0000 Buried
197 S C 0.0000 Buried
198 T C -0.5745 Soluble
199 L C 0.0000 Buried
200 T C -0.3046 Soluble
201 L C -0.6484 Soluble
202 S C -1.0230 Soluble
203 K C -1.9943 Soluble
204 A C -1.8535 Soluble
205 D C -2.5177 Soluble
206 Y C 0.0000 Buried
207 E C -3.4337 Soluble
208 K C -3.6400 Soluble
209 H C -3.2000 Soluble
210 K C -3.3928 Soluble
211 V C -1.8234 Soluble
212 Y C 0.0000 Buried
213 A C 0.0000 Buried
214 C C 0.0000 Buried
215 E C -0.9098 Soluble
216 V C 0.0000 Buried
217 T C -0.7497 Soluble
218 H C 0.0000 Buried
219 Q C -1.4367 Soluble
220 G C -0.1751 Soluble
221 L C -0.1275 Soluble
222 S C -0.4525 Soluble
223 S C -0.4242 Soluble
224 P C -0.3741 Soluble
225 V C -0.0990 Soluble
226 T C -0.5280 Soluble
227 K C -0.5848 Soluble
228 S C -0.6446 Soluble
229 F C -0.9770 Soluble
230 N C -2.2565 Soluble
231 R C -2.5825 Soluble
232 G C -1.5601 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6652 2.3071 CSV PDB
4.5 -0.701 2.2368 CSV PDB
5.0 -0.7434 2.2297 CSV PDB
5.5 -0.7842 2.2297 CSV PDB
6.0 -0.8139 2.2297 CSV PDB
6.5 -0.8248 2.2297 CSV PDB
7.0 -0.8164 2.2296 CSV PDB
7.5 -0.795 2.2294 CSV PDB
8.0 -0.766 2.2288 CSV PDB
8.5 -0.7315 2.227 CSV PDB
9.0 -0.6915 2.2212 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH