Diridavumab / 3gbm

A4DAB087_3gbm_I_M | Source PDB: 3gbm | Chains: I, M | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.592
Max Score
3.0411
Min Score
-3.2972
Total Score
-242.72
Score CDR
-0.2694
Score non-CDR
-0.6777

Calculation Parameters

Chain Sequence(s) EVQLVESGAEVKKPGSSVKVSCKASGGPFRSYAISWVRQAPGQGPEWMGGIIPIFGTTKYAPKFQGRVTITADDFAGTVYMELSSLRSEDTAMYYCAKHMGYQVRETMDVWGKGTTVTVSSASTKGPSVFPLAPSSTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVVLTQPPSVSAAPGQKVTISCSGSSSNIGNDYVSWYQQLPGTAPKLLIYDNNKRPSGIPDRFSGSKSGTSATLGITGLQTGDEANYYCATWDRTAYVVFGGGTKLTVLAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKAAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHESTVEKTVAP input.pdb
Selected Chain(s) I (Heavy), M (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.2972
Maximum Score 3.0411
Average Score -0.592
Total Score -242.7247

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E I -2.0498 Soluble
2 V I -1.2947 Soluble
3 Q I -1.6405 Soluble
4 L I 0.0000 Buried
5 V I 0.3785 Aggregation-prone
6 E I -0.3355 Soluble
7 S I -0.4963 Soluble
8 G I -0.4637 Soluble
9 A I -0.1938 Soluble
11 E I -0.3623 Soluble
12 V I 0.1558 Aggregation-prone
13 K I -1.2215 Soluble
14 K I -2.2897 Soluble
15 P I -2.1228 Soluble
16 G I -1.5875 Soluble
17 S I -1.2216 Soluble
18 S I -1.4312 Soluble
19 V I 0.0000 Buried
20 K I -2.0335 Soluble
21 V I 0.0000 Buried
22 S I -0.7171 Soluble
23 C I 0.0000 Buried
24 K I -1.6719 Soluble
25 A I 0.0000 Buried
26 S I -1.2494 Soluble
27 G I -1.1102 Soluble
28 G I -0.1061 Soluble
29 P I 0.0843 Aggregation-prone
30 F I 1.5536 Aggregation-prone
35 R I 0.7228 Aggregation-prone
36 S I 0.7329 Aggregation-prone
37 Y I 0.9137 Aggregation-prone
38 A I 0.0000 Buried
39 I I 0.0000 Buried
40 S I 0.0000 Buried
41 W I 0.0000 Buried
42 V I 0.0000 Buried
43 R I 0.0000 Buried
44 Q I -0.6039 Soluble
45 A I -1.0134 Soluble
46 P I -1.0377 Soluble
47 G I -1.2134 Soluble
48 Q I -1.7252 Soluble
49 G I -1.0886 Soluble
50 P I 0.0000 Buried
51 E I -0.7160 Soluble
52 W I 0.0000 Buried
53 M I 0.0000 Buried
54 G I 0.0000 Buried
55 G I 0.0000 Buried
56 I I 0.0000 Buried
57 I I 1.1326 Aggregation-prone
58 P I 0.0000 Buried
59 I I 3.0411 Aggregation-prone
62 F I 2.9274 Aggregation-prone
63 G I 1.1978 Aggregation-prone
64 T I 0.5706 Aggregation-prone
65 T I -0.1249 Soluble
66 K I -0.7488 Soluble
67 Y I -0.8584 Soluble
68 A I 0.0000 Buried
69 P I -1.4273 Soluble
70 K I -2.1939 Soluble
71 F I 0.0000 Buried
72 Q I -1.9811 Soluble
74 G I -1.4611 Soluble
75 R I -1.4438 Soluble
76 V I 0.0000 Buried
77 T I -0.9942 Soluble
78 I I 0.0000 Buried
79 T I -0.5429 Soluble
80 A I -0.5446 Soluble
81 D I -0.7701 Soluble
82 D I -0.4797 Soluble
83 F I 1.2631 Aggregation-prone
84 A I 0.0704 Aggregation-prone
85 G I -0.5458 Soluble
86 T I 0.0000 Buried
87 V I 0.0000 Buried
88 Y I -0.7804 Soluble
89 M I 0.0000 Buried
90 E I -1.8763 Soluble
91 L I 0.0000 Buried
92 S I -1.2694 Soluble
93 S I -1.2324 Soluble
94 L I 0.0000 Buried
95 R I -3.0770 Soluble
96 S I -2.4217 Soluble
97 E I -2.5683 Soluble
98 D I 0.0000 Buried
99 T I -0.9958 Soluble
100 A I 0.0000 Buried
101 M I 0.0117 Aggregation-prone
102 Y I 0.0000 Buried
103 Y I 0.0000 Buried
104 C I 0.0000 Buried
105 A I 0.0000 Buried
106 K I -0.1042 Soluble
107 H I 0.0000 Buried
108 M I 0.3352 Aggregation-prone
109 G I 0.3114 Aggregation-prone
110 Y I 1.4544 Aggregation-prone
111 Q I -0.0005 Soluble
112A V I 0.0000 Buried
112 R I -0.2457 Soluble
113 E I 0.0000 Buried
114 T I 0.0000 Buried
115 M I 0.0000 Buried
116 D I -0.5421 Soluble
117 V I -0.6653 Soluble
118 W I -0.4133 Soluble
119 G I 0.0000 Buried
120 K I -1.4697 Soluble
121 G I 0.0000 Buried
122 T I 0.0000 Buried
123 T I 0.0046 Aggregation-prone
124 V I 0.0000 Buried
125 T I -0.6386 Soluble
126 V I 0.0000 Buried
127 S I -1.2835 Soluble
128 S I -1.2483 Soluble
129 A I -0.7886 Soluble
130 S I -0.8506 Soluble
131 T I -0.9829 Soluble
132 K I -1.5755 Soluble
133 G I -1.5136 Soluble
134 P I -0.6473 Soluble
135 S I -0.2547 Soluble
136 V I 0.0000 Buried
137 F I 0.0000 Buried
138 P I -0.5470 Soluble
139 L I 0.0000 Buried
140 A I 0.0042 Aggregation-prone
141 P I -0.0748 Soluble
142 S I -0.3363 Soluble
143 S I -0.2658 Soluble
144 T I -0.2127 Soluble
145 A I -0.2007 Soluble
146 A I -0.1780 Soluble
147 L I 0.0000 Buried
148 G I 0.0000 Buried
149 C I 0.0000 Buried
150 L I 0.0000 Buried
151 V I 0.0000 Buried
152 K I 0.0000 Buried
153 D I -0.4536 Soluble
154 Y I 0.0000 Buried
155 F I 0.0000 Buried
156 P I 0.0000 Buried
157 E I -0.7989 Soluble
158 P I -0.9744 Soluble
159 V I -0.6136 Soluble
160 T I -0.7414 Soluble
161 V I -0.2642 Soluble
162 S I -0.3940 Soluble
163 W I 0.0000 Buried
164 N I -0.7708 Soluble
165 S I -0.6565 Soluble
166 G I -0.5262 Soluble
167 A I -0.2183 Soluble
168 L I -0.0026 Soluble
169 T I -0.1678 Soluble
170 S I -0.2281 Soluble
171 G I -0.2560 Soluble
172 V I 0.2459 Aggregation-prone
173 H I -0.0640 Soluble
174 T I -0.0015 Soluble
175 F I 0.0000 Buried
176 P I -0.0581 Soluble
177 A I 0.1758 Aggregation-prone
178 V I 0.0000 Buried
179 L I 0.9673 Aggregation-prone
180 Q I 0.2582 Aggregation-prone
181 S I -0.1233 Soluble
182 S I -0.1873 Soluble
183 G I 0.0601 Aggregation-prone
184 L I -0.0406 Soluble
185 Y I 0.2090 Aggregation-prone
186 S I 0.0000 Buried
187 L I 0.0000 Buried
188 S I 0.0000 Buried
189 S I 0.0000 Buried
190 V I 0.1590 Aggregation-prone
191 V I 0.0000 Buried
192 T I -0.1948 Soluble
193 V I 0.0000 Buried
194 P I -0.5319 Soluble
195 S I -0.4286 Soluble
196 S I -0.5400 Soluble
197 S I -0.6167 Soluble
198 L I -0.3758 Soluble
199 G I -0.7041 Soluble
200 T I -0.7449 Soluble
201 Q I -1.1949 Soluble
202 T I -0.8042 Soluble
203 Y I 0.0000 Buried
204 I I -1.1422 Soluble
205 C I 0.0000 Buried
206 N I -1.6087 Soluble
207 V I 0.0000 Buried
208 N I -2.1325 Soluble
209 H I 0.0000 Buried
210 K I -2.7571 Soluble
211 P I -1.7312 Soluble
212 S I -1.9102 Soluble
213 N I -2.5929 Soluble
214 T I -2.2715 Soluble
215 K I -2.7690 Soluble
216 V I -1.6185 Soluble
217 D I -2.6062 Soluble
218 K I -1.8902 Soluble
219 R I -2.1383 Soluble
220 V I -0.5889 Soluble
3 V M 1.3641 Aggregation-prone
4 L M 0.0000 Buried
5 T M 0.1201 Aggregation-prone
6 Q M 0.0000 Buried
7 P M -0.6456 Soluble
8 P M -0.9354 Soluble
9 S M -1.0025 Soluble
11 V M -0.4095 Soluble
12 S M -0.1680 Soluble
13 A M -0.4744 Soluble
14 A M -0.6130 Soluble
15 P M -1.2719 Soluble
16 G M -1.8804 Soluble
17 Q M -2.5358 Soluble
18 K M -2.6531 Soluble
19 V M 0.0000 Buried
20 T M -0.5056 Soluble
21 I M 0.0000 Buried
22 S M -0.3079 Soluble
23 C M 0.0000 Buried
24 S M -0.1180 Soluble
25 G M -0.2219 Soluble
26 S M -0.6465 Soluble
27 S M -1.0226 Soluble
28 S M -1.6181 Soluble
29 N M 0.0000 Buried
30 I M 0.0000 Buried
35 G M -1.2576 Soluble
36 N M -1.9354 Soluble
37 D M -1.0691 Soluble
38 Y M 0.1434 Aggregation-prone
39 V M 0.0000 Buried
40 S M 0.0000 Buried
41 W M 0.0000 Buried
42 Y M 0.0000 Buried
43 Q M 0.0000 Buried
44 Q M 0.0000 Buried
45 L M -0.0747 Soluble
46 P M -0.3477 Soluble
47 G M -0.6545 Soluble
48 T M -0.6324 Soluble
49 A M 0.0000 Buried
50 P M 0.0000 Buried
51 K M -0.8277 Soluble
52 L M 0.0000 Buried
53 L M 0.0000 Buried
54 I M 0.0000 Buried
55 Y M -0.8131 Soluble
56 D M -0.8396 Soluble
57 N M -1.2611 Soluble
65 N M -2.1926 Soluble
66 K M -2.4664 Soluble
67 R M -2.1439 Soluble
68 P M -0.9595 Soluble
69 S M -0.9311 Soluble
70 G M -0.8127 Soluble
71 I M -1.0135 Soluble
72 P M -1.3174 Soluble
74 D M -2.3680 Soluble
75 R M -1.5678 Soluble
76 F M 0.0000 Buried
77 S M -1.4608 Soluble
78 G M -1.2615 Soluble
79 S M -1.1395 Soluble
80 K M -1.0760 Soluble
83 S M -0.7991 Soluble
84 G M -1.0004 Soluble
85 T M -0.9822 Soluble
86 S M -0.6226 Soluble
87 A M 0.0000 Buried
88 T M -0.5394 Soluble
89 L M 0.0000 Buried
90 G M 0.0000 Buried
91 I M 0.0000 Buried
92 T M -1.9291 Soluble
93 G M -1.6343 Soluble
94 L M 0.0000 Buried
95 Q M -1.9601 Soluble
96 T M -1.1069 Soluble
97 G M -1.1907 Soluble
98 D M 0.0000 Buried
99 E M -1.7758 Soluble
100 A M 0.0000 Buried
101 N M -1.1881 Soluble
102 Y M 0.0000 Buried
103 Y M 0.0000 Buried
104 C M 0.0000 Buried
105 A M 0.0000 Buried
106 T M 0.0000 Buried
107 W M -0.7307 Soluble
108 D M -1.8096 Soluble
109 R M -2.1248 Soluble
113 T M -0.5935 Soluble
114 A M -0.9499 Soluble
115 Y M 0.0000 Buried
116 V M 0.0000 Buried
117 V M 0.0000 Buried
118 F M 0.2606 Aggregation-prone
119 G M 0.0000 Buried
120 G M -0.5263 Soluble
121 G M -1.0626 Soluble
122 T M 0.0000 Buried
123 K M -2.1641 Soluble
124 L M 0.0000 Buried
125 T M -0.5155 Soluble
126 V M 0.0000 Buried
127 L M 1.1718 Aggregation-prone
128 A M -0.0316 Soluble
129 P M -0.2870 Soluble
130 S M -0.5619 Soluble
131 V M 0.0000 Buried
132 T M -0.4603 Soluble
133 L M 0.0000 Buried
134 F M 0.0000 Buried
135 P M -0.0315 Soluble
136 P M 0.0000 Buried
137 S M -0.7441 Soluble
138 S M -1.3140 Soluble
139 E M -2.0031 Soluble
140 E M 0.0000 Buried
141 L M -1.6837 Soluble
142 Q M -2.1620 Soluble
143 A M -1.6928 Soluble
144 N M -2.3062 Soluble
145 K M -1.8688 Soluble
146 A M 0.0000 Buried
147 T M 0.0000 Buried
148 L M 0.0000 Buried
149 V M 0.0000 Buried
150 C M 0.0000 Buried
151 L M 0.0000 Buried
152 I M 0.0000 Buried
153 S M -0.9937 Soluble
154 D M -2.1445 Soluble
155 F M 0.0000 Buried
156 Y M 0.3456 Aggregation-prone
157 P M -0.0116 Soluble
158 G M -0.3724 Soluble
159 A M 0.0904 Aggregation-prone
160 V M 0.1601 Aggregation-prone
161 T M -0.0216 Soluble
162 V M 0.3059 Aggregation-prone
163 A M -0.2177 Soluble
164 W M -0.6271 Soluble
165 K M -1.6928 Soluble
166 A M -1.4096 Soluble
167 A M -0.1909 Soluble
168 G M -0.1930 Soluble
169 V M 0.2284 Aggregation-prone
170 E M -0.0920 Soluble
171 T M 0.0786 Aggregation-prone
172 T M -0.0080 Soluble
173 T M -0.0995 Soluble
174 P M -0.4498 Soluble
175 S M -1.2238 Soluble
176 K M -2.5965 Soluble
177 Q M -2.3539 Soluble
178 S M -1.9089 Soluble
179 N M -2.7127 Soluble
180 N M -2.8183 Soluble
181 K M -2.3163 Soluble
182 Y M -1.6744 Soluble
183 A M 0.0000 Buried
184 A M 0.0000 Buried
185 S M 0.0000 Buried
186 S M 0.0000 Buried
187 Y M 0.0000 Buried
188 L M 0.0000 Buried
189 S M -0.3625 Soluble
190 L M -0.7439 Soluble
191 T M -1.5372 Soluble
192 P M -2.1406 Soluble
193 E M -3.1380 Soluble
194 Q M -2.4313 Soluble
195 W M 0.0000 Buried
196 K M -3.2972 Soluble
197 S M -2.6498 Soluble
198 H M -2.8300 Soluble
199 R M -2.5920 Soluble
200 S M -1.6269 Soluble
201 Y M 0.0000 Buried
202 S M -1.1123 Soluble
203 C M 0.0000 Buried
204 Q M -0.9748 Soluble
205 V M 0.0000 Buried
206 T M -0.5508 Soluble
207 H M -0.7321 Soluble
208 E M -1.9173 Soluble
209 S M -0.7621 Soluble
210 T M -0.5597 Soluble
211 V M -0.1722 Soluble
212 E M -1.3661 Soluble
213 K M -1.8856 Soluble
214 T M -0.8584 Soluble
215 V M -0.2309 Soluble
216 A M -0.7535 Soluble
217 P M -0.9650 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.5608 5.9221 CSV PDB
4.5 -0.5904 5.9221 CSV PDB
5.0 -0.625 5.9221 CSV PDB
5.5 -0.6559 5.9221 CSV PDB
6.0 -0.6731 5.9221 CSV PDB
6.5 -0.6692 5.9221 CSV PDB
7.0 -0.6447 5.9221 CSV PDB
7.5 -0.6072 5.9221 CSV PDB
8.0 -0.5633 5.9221 CSV PDB
8.5 -0.5154 5.922 CSV PDB
9.0 -0.4635 5.9217 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH