Tomuzotuximab / 5th2

A4DAB272_5th2_D_C | Source PDB: 5th2 | Chains: D, C | Done
99.56
Yes
10 Å
Yes
Avg A4D Score
-0.6745
Max Score
1.8872
Min Score
-3.7749
Total Score
-287.33
Score CDR
-0.2746
Score non-CDR
-0.7229

Calculation Parameters

Chain Sequence(s) QVQLKQSGPGLVQPSQSLSITCTVSGFSLTNYGVHWVRQSPGKGLEWLGVIWSGGNTDYNTPFTSRLSINKDNSKSQVFFKMNLQSNDTAIYYCARALTYYDYEFAYWGQGTLVTVSAASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKDILLTQSPVILSVSPGERVSFSCRASQSIGTNIHWYQQRTNGSPRLLIKYASESISGIPSRFSGSGSGTDFTLSINSVESEDIADYYCQQNNNWPTTFGAGTKLELKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYAEVTHQGLSSPVTKSFNRGA input.pdb
Selected Chain(s) D (Heavy), C (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.7749
Maximum Score 1.8872
Average Score -0.6745
Total Score -287.3325

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q D -1.4356 Soluble
2 V D -0.8214 Soluble
3 Q D -1.4198 Soluble
4 L D 0.0000 Buried
5 K D -1.8800 Soluble
6 Q D 0.0000 Buried
7 S D -0.8214 Soluble
8 G D -1.1062 Soluble
9 P D -0.4898 Soluble
11 G D -0.3599 Soluble
12 L D 0.0142 Aggregation-prone
13 V D 0.0000 Buried
14 Q D -1.2369 Soluble
15 P D -1.5488 Soluble
16 S D -1.5666 Soluble
17 Q D -1.9730 Soluble
18 S D -1.9001 Soluble
19 L D 0.0000 Buried
20 S D -0.8762 Soluble
21 I D 0.0000 Buried
22 T D -0.4768 Soluble
23 C D 0.0000 Buried
24 T D -0.9690 Soluble
25 V D 0.0000 Buried
26 S D -1.0793 Soluble
27 G D -0.9403 Soluble
28 F D -0.5192 Soluble
29 S D -0.8611 Soluble
30 L D 0.0000 Buried
35 T D -0.8931 Soluble
36 N D -0.8432 Soluble
37 Y D 0.4170 Aggregation-prone
38 G D 0.0000 Buried
39 V D 0.0000 Buried
40 H D 0.0000 Buried
41 W D 0.0000 Buried
42 V D 0.0000 Buried
43 R D 0.0000 Buried
44 Q D -0.7270 Soluble
45 S D -1.1587 Soluble
46 P D -1.2665 Soluble
47 G D -1.7454 Soluble
48 K D -2.2495 Soluble
49 G D -1.2396 Soluble
50 L D 0.0000 Buried
51 E D 0.0000 Buried
52 W D 0.0000 Buried
53 L D 0.0000 Buried
54 G D 0.0000 Buried
55 V D 0.0000 Buried
56 I D 0.0000 Buried
57 W D -0.5161 Soluble
58 S D -0.6225 Soluble
59 G D -1.2087 Soluble
63 G D -1.5184 Soluble
64 N D -1.8511 Soluble
65 T D -1.3269 Soluble
66 D D -1.0763 Soluble
67 Y D -0.4622 Soluble
68 N D -0.2177 Soluble
69 T D -0.2554 Soluble
70 P D -0.4101 Soluble
71 F D 0.0000 Buried
72 T D -0.5244 Soluble
74 S D -0.7170 Soluble
75 R D -1.3423 Soluble
76 L D 0.0000 Buried
77 S D -1.2921 Soluble
78 I D 0.0000 Buried
79 N D -1.7528 Soluble
80 K D -1.4028 Soluble
81 D D -1.7689 Soluble
82 N D -2.1920 Soluble
83 S D -1.6677 Soluble
84 K D -2.3467 Soluble
85 S D -1.5578 Soluble
86 Q D -1.4145 Soluble
87 V D 0.0000 Buried
88 F D -0.2596 Soluble
89 F D 0.0000 Buried
90 K D -2.0331 Soluble
91 M D 0.0000 Buried
92 N D -2.3812 Soluble
94 L D 0.0000 Buried
95 Q D -2.2273 Soluble
96 S D -1.5642 Soluble
97 N D -2.1088 Soluble
98 D D 0.0000 Buried
99 T D -0.7295 Soluble
100 A D -0.5012 Soluble
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 A D 0.0000 Buried
106 R D 0.0000 Buried
107 A D 0.0000 Buried
108 L D 1.0091 Aggregation-prone
109 T D 1.1100 Aggregation-prone
110 Y D 1.8125 Aggregation-prone
111 Y D 1.8872 Aggregation-prone
112 D D 0.9847 Aggregation-prone
113 Y D 0.5018 Aggregation-prone
114 E D 0.0000 Buried
115 F D 0.0000 Buried
116 A D 0.4335 Aggregation-prone
117 Y D 0.3283 Aggregation-prone
118 W D -0.2991 Soluble
119 G D 0.0000 Buried
120 Q D -1.7797 Soluble
121 G D -1.0028 Soluble
122 T D 0.0000 Buried
123 L D 0.2349 Aggregation-prone
124 V D 0.0000 Buried
125 T D 0.0000 Buried
126 V D 0.0000 Buried
127 S D -0.6137 Soluble
128 A D -0.2505 Soluble
129 A D -0.2472 Soluble
130 S D -0.4404 Soluble
131 T D -0.6760 Soluble
132 K D -1.1170 Soluble
133 G D -1.2391 Soluble
134 P D 0.0000 Buried
135 S D -0.2402 Soluble
136 V D 0.0000 Buried
137 F D 0.0000 Buried
138 P D -1.1945 Soluble
139 L D 0.0000 Buried
140 A D -1.3858 Soluble
141 P D 0.0000 Buried
142 S D -1.2761 Soluble
143 S D -1.0613 Soluble
144 K D -1.4853 Soluble
145 S D 0.0000 Buried
146 T D -0.8439 Soluble
147 S D -0.8894 Soluble
148 G D -0.8515 Soluble
149 G D -0.9117 Soluble
150 T D -0.6373 Soluble
151 A D 0.0000 Buried
152 A D 0.0000 Buried
153 L D 0.0000 Buried
154 G D 0.0000 Buried
155 C D 0.0000 Buried
156 L D 0.0000 Buried
157 V D 0.0000 Buried
158 K D 0.0000 Buried
159 D D -0.4195 Soluble
160 Y D 0.0000 Buried
161 F D 0.0000 Buried
162 P D 0.0000 Buried
163 E D -0.2389 Soluble
164 P D -0.4324 Soluble
165 V D -0.5010 Soluble
166 T D -0.4409 Soluble
167 V D -0.1408 Soluble
168 S D -0.2591 Soluble
169 W D 0.0000 Buried
170 N D -0.7077 Soluble
171 S D -0.5670 Soluble
172 G D -0.5589 Soluble
173 A D -0.2710 Soluble
174 L D -0.0356 Soluble
175 T D -0.1916 Soluble
176 S D -0.2004 Soluble
177 G D -0.2538 Soluble
178 V D 0.1631 Aggregation-prone
179 H D -0.3007 Soluble
180 T D 0.0615 Aggregation-prone
181 F D 0.0000 Buried
182 P D -0.2755 Soluble
183 A D 0.2728 Aggregation-prone
184 V D 0.5041 Aggregation-prone
185 L D 0.9952 Aggregation-prone
186 Q D 0.1770 Aggregation-prone
187 S D -0.1224 Soluble
188 S D -0.2645 Soluble
189 G D -0.0292 Soluble
190 L D 0.0852 Aggregation-prone
191 Y D 0.3983 Aggregation-prone
192 S D 0.0000 Buried
193 L D 0.0000 Buried
194 S D 0.0000 Buried
195 S D 0.0000 Buried
196 V D 0.0000 Buried
197 V D 0.0000 Buried
198 T D -0.1316 Soluble
199 V D 0.0000 Buried
200 P D -0.5343 Soluble
201 S D -0.5729 Soluble
202 S D -0.4669 Soluble
203 S D -0.2643 Soluble
204 L D -0.4474 Soluble
205 G D -0.6909 Soluble
206 T D -0.2555 Soluble
208 T D -0.6994 Soluble
209 Y D -0.7755 Soluble
210 I D -1.0362 Soluble
211 C D 0.0000 Buried
212 N D -0.9657 Soluble
213 V D 0.0000 Buried
214 N D -1.5641 Soluble
215 H D 0.0000 Buried
216 K D -2.5223 Soluble
217 P D -1.3976 Soluble
218 S D -1.6775 Soluble
219 N D -2.4197 Soluble
220 T D -1.9119 Soluble
221 K D -2.2666 Soluble
222 V D -0.9983 Soluble
223 D D -1.2769 Soluble
224 K D -1.5633 Soluble
225 R D -2.3683 Soluble
226 V D 0.0000 Buried
227 E D -2.7332 Soluble
228 P D -1.8775 Soluble
229 K D -2.4348 Soluble
1 D C -1.3412 Soluble
2 I C 0.0000 Buried
3 L C 0.6104 Aggregation-prone
4 L C 0.0000 Buried
5 T C -0.4140 Soluble
6 Q C -0.0868 Soluble
7 S C 0.0273 Aggregation-prone
8 P C 0.7750 Aggregation-prone
9 V C 1.8550 Aggregation-prone
10 I C 1.3742 Aggregation-prone
11 L C 0.5903 Aggregation-prone
12 S C -0.3948 Soluble
13 V C 0.0000 Buried
14 S C -1.9142 Soluble
15 P C -2.1486 Soluble
16 G C -2.0838 Soluble
17 E C -2.6758 Soluble
18 R C -3.0274 Soluble
19 V C 0.0000 Buried
20 S C -0.6848 Soluble
21 F C 0.0000 Buried
23 C C 0.0000 Buried
24 R C -2.4716 Soluble
25 A C -1.2739 Soluble
26 S C -0.9938 Soluble
27 Q C -1.3323 Soluble
28 S C -1.5100 Soluble
29 I C 0.0000 Buried
36 G C -1.0236 Soluble
37 T C -0.7851 Soluble
38 N C -0.8147 Soluble
39 I C 0.0000 Buried
40 H C 0.0000 Buried
41 W C 0.0000 Buried
42 Y C 0.0000 Buried
43 Q C -0.6366 Soluble
44 Q C -1.1418 Soluble
45 R C -1.8344 Soluble
46 T C -1.7128 Soluble
47 N C -1.9947 Soluble
48 G C -1.4512 Soluble
49 S C -0.9197 Soluble
50 P C -0.5796 Soluble
52 L C 0.1900 Aggregation-prone
53 L C 0.0000 Buried
54 I C 0.0000 Buried
55 K C -0.6795 Soluble
56 Y C -0.5589 Soluble
57 A C 0.0000 Buried
65 S C -1.1011 Soluble
66 E C -1.6778 Soluble
67 S C -0.9840 Soluble
68 I C -0.3746 Soluble
69 S C -0.2689 Soluble
70 G C -0.3634 Soluble
71 I C 0.0430 Aggregation-prone
72 P C -0.2576 Soluble
74 S C -0.4895 Soluble
75 R C -1.0100 Soluble
76 F C 0.0000 Buried
77 S C -0.7356 Soluble
78 G C -0.6675 Soluble
79 S C -0.7969 Soluble
80 G C -1.0651 Soluble
83 S C -1.1349 Soluble
84 G C -1.3219 Soluble
85 T C -1.8635 Soluble
86 D C -2.4311 Soluble
87 F C 0.0000 Buried
88 T C -0.8236 Soluble
89 L C 0.0000 Buried
90 S C -0.7854 Soluble
91 I C 0.0000 Buried
92 N C -2.0530 Soluble
93 S C -2.1536 Soluble
94 V C 0.0000 Buried
95 E C -2.7670 Soluble
96 S C -2.3525 Soluble
97 E C -2.1584 Soluble
98 D C 0.0000 Buried
99 I C -1.4412 Soluble
100 A C 0.0000 Buried
101 D C -0.7794 Soluble
102 Y C 0.0000 Buried
103 Y C 0.0000 Buried
104 C C 0.0000 Buried
105 Q C 0.0000 Buried
106 Q C 0.0000 Buried
107 N C -0.6456 Soluble
108 N C -1.1803 Soluble
109 N C -0.9611 Soluble
114 W C 0.1301 Aggregation-prone
115 P C -0.2213 Soluble
116 T C 0.0000 Buried
117 T C -0.0115 Soluble
118 F C 0.1974 Aggregation-prone
119 G C 0.0000 Buried
120 A C 0.0099 Aggregation-prone
121 G C 0.0804 Aggregation-prone
122 T C 0.0000 Buried
123 K C -0.2017 Soluble
124 L C 0.0000 Buried
125 E C -0.8735 Soluble
126 L C 0.0000 Buried
127 K C -1.8038 Soluble
128 R C -1.2805 Soluble
129 T C -0.1300 Soluble
130 V C 0.4746 Aggregation-prone
131 A C 0.0399 Aggregation-prone
132 A C -0.1126 Soluble
133 P C 0.0000 Buried
134 S C -0.2430 Soluble
135 V C 0.0000 Buried
136 F C 0.0000 Buried
137 I C 0.0000 Buried
138 F C 0.0000 Buried
139 P C -0.7333 Soluble
140 P C 0.0000 Buried
141 S C -1.8768 Soluble
142 D C -3.1261 Soluble
143 E C -3.0227 Soluble
144 Q C 0.0000 Buried
145 L C -2.2990 Soluble
146 K C -2.8204 Soluble
147 S C -1.7459 Soluble
148 G C -1.3742 Soluble
149 T C -1.0333 Soluble
150 A C 0.0000 Buried
151 S C 0.0000 Buried
152 V C 0.0000 Buried
153 V C 0.0000 Buried
154 C C 0.0000 Buried
155 L C 0.0000 Buried
156 L C 0.0000 Buried
157 N C 0.0000 Buried
158 N C -0.9368 Soluble
159 F C 0.0000 Buried
160 Y C 0.0000 Buried
161 P C -1.4828 Soluble
162 R C -2.4165 Soluble
163 E C -2.8973 Soluble
164 A C -2.0534 Soluble
165 K C -2.2638 Soluble
166 V C -1.2322 Soluble
167 Q C -1.2611 Soluble
168 W C 0.0000 Buried
169 K C -1.3306 Soluble
170 V C 0.0000 Buried
171 D C -2.4142 Soluble
173 A C -0.3086 Soluble
174 L C 0.2686 Aggregation-prone
175 Q C -0.3730 Soluble
176 S C -0.5982 Soluble
177 G C -0.9308 Soluble
178 N C -0.8786 Soluble
179 S C -1.1114 Soluble
180 Q C -1.3268 Soluble
181 E C -1.5989 Soluble
182 S C -0.7861 Soluble
183 V C -0.5097 Soluble
184 T C -1.0152 Soluble
185 E C -2.2655 Soluble
186 Q C 0.0000 Buried
187 D C -1.9833 Soluble
188 S C -2.3135 Soluble
189 K C -2.6607 Soluble
190 D C -1.9313 Soluble
191 S C 0.0000 Buried
192 T C 0.0000 Buried
193 Y C 0.0000 Buried
194 S C 0.0000 Buried
195 L C 0.0000 Buried
196 S C 0.0000 Buried
197 S C 0.0000 Buried
198 T C -0.6356 Soluble
199 L C 0.0000 Buried
200 T C -0.4633 Soluble
201 L C -0.7215 Soluble
202 S C -1.2757 Soluble
203 K C -2.1264 Soluble
204 A C -1.9595 Soluble
205 D C -2.6459 Soluble
206 Y C 0.0000 Buried
207 E C -3.5669 Soluble
208 K C -3.7749 Soluble
209 H C -3.2261 Soluble
210 K C -3.1707 Soluble
211 V C -1.5698 Soluble
212 Y C 0.0000 Buried
213 A C -1.1914 Soluble
215 E C -1.7161 Soluble
216 V C 0.0000 Buried
217 T C -1.3287 Soluble
218 H C 0.0000 Buried
219 Q C -1.6483 Soluble
220 G C -0.4018 Soluble
221 L C -0.2199 Soluble
222 S C -0.4579 Soluble
223 S C -0.4437 Soluble
224 P C -0.7945 Soluble
225 V C -0.2719 Soluble
226 T C -0.7706 Soluble
227 K C -1.1082 Soluble
228 S C -0.9202 Soluble
229 F C 0.0000 Buried
230 N C -1.7110 Soluble
231 R C -2.1357 Soluble
232 G C -1.3163 Soluble
233 A C -0.7368 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6795 2.8641 CSV PDB
4.5 -0.7155 2.8087 CSV PDB
5.0 -0.7586 2.7357 CSV PDB
5.5 -0.7995 2.6542 CSV PDB
6.0 -0.8273 2.5696 CSV PDB
6.5 -0.8337 2.484 CSV PDB
7.0 -0.819 2.3981 CSV PDB
7.5 -0.7902 2.3127 CSV PDB
8.0 -0.7541 2.2287 CSV PDB
8.5 -0.7134 2.2018 CSV PDB
9.0 -0.6684 2.2517 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH