Zemocimig / 8gv0

A4DAB305_8gv0_E_F | Source PDB: 8gv0 | Chains: E, F | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7768
Max Score
1.5708
Min Score
-3.4832
Total Score
-334.03
Score CDR
-0.5608
Score non-CDR
-0.8316

Calculation Parameters

Chain Sequence(s) QVQLVESGGGLVQPGGSLRLSCAASGFTFSYYDIQWVRQAPGKGLEWVSSISPSGQSTYYRREVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARRTGRIFDYWGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYTCNVDHKPSNTKVDKRVEEIVLTQSPATLSVSPGERATLSCRASRSVRRELAWYQQKPGQAPELLIYGASTRETGIPARFSGSGSGTDFTLTINSLEAEDAATYYCQQYRDPPGTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) E (Heavy), F (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.4832
Maximum Score 1.5708
Average Score -0.7768
Total Score -334.029

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q E -1.0944 Soluble
2 V E -0.3730 Soluble
3 Q E -0.6943 Soluble
4 L E 0.0000 Buried
5 V E 1.0708 Aggregation-prone
6 E E 0.0000 Buried
7 S E -0.1257 Soluble
8 G E -0.5710 Soluble
9 G E -0.3600 Soluble
11 G E 0.0000 Buried
12 L E -0.1192 Soluble
13 V E 0.0000 Buried
14 Q E -1.6426 Soluble
15 P E -1.7164 Soluble
16 G E -1.3392 Soluble
17 G E -1.0701 Soluble
18 S E -1.2348 Soluble
19 L E -1.0516 Soluble
20 R E -2.1249 Soluble
21 L E 0.0000 Buried
22 S E -0.4824 Soluble
23 C E 0.0000 Buried
24 A E -0.0954 Soluble
25 A E 0.0676 Aggregation-prone
26 S E -0.1442 Soluble
27 G E -0.0902 Soluble
28 F E 1.5708 Aggregation-prone
29 T E 1.1284 Aggregation-prone
30 F E 0.0000 Buried
35 S E 0.2674 Aggregation-prone
36 Y E 1.4529 Aggregation-prone
37 Y E 0.8983 Aggregation-prone
38 D E 0.0000 Buried
39 I E 0.0000 Buried
40 Q E 0.0000 Buried
41 W E 0.0000 Buried
42 V E 0.0000 Buried
43 R E 0.0000 Buried
44 Q E -0.7149 Soluble
45 A E -1.1139 Soluble
46 P E -0.9310 Soluble
47 G E -1.5229 Soluble
48 K E -2.2575 Soluble
49 G E -1.3806 Soluble
50 L E 0.0000 Buried
51 E E -0.8050 Soluble
52 W E 0.0000 Buried
53 V E 0.0000 Buried
54 S E 0.0000 Buried
55 S E 0.0000 Buried
56 I E 0.0000 Buried
57 S E -0.3728 Soluble
58 P E 0.0000 Buried
59 S E -0.5260 Soluble
62 G E -1.0950 Soluble
63 Q E -1.8302 Soluble
64 S E -0.8674 Soluble
65 T E -0.1196 Soluble
66 Y E 0.3384 Aggregation-prone
67 Y E -1.0218 Soluble
68 R E -2.2072 Soluble
69 R E -3.4509 Soluble
70 E E -3.3328 Soluble
71 V E 0.0000 Buried
72 K E -3.1130 Soluble
74 G E -2.0459 Soluble
75 R E -1.6418 Soluble
76 F E 0.0000 Buried
77 T E -0.8807 Soluble
78 I E 0.0000 Buried
79 S E -0.7924 Soluble
80 R E -1.4694 Soluble
81 D E -2.0385 Soluble
82 N E -1.9320 Soluble
83 S E -1.7018 Soluble
84 K E -2.4832 Soluble
85 N E -1.9106 Soluble
86 T E 0.0000 Buried
87 L E 0.0000 Buried
88 Y E 0.0000 Buried
89 L E 0.0000 Buried
90 Q E -1.4308 Soluble
91 M E 0.0000 Buried
92 N E -1.6520 Soluble
93 S E -1.2619 Soluble
94 L E 0.0000 Buried
95 R E -2.1413 Soluble
96 A E -1.6559 Soluble
97 E E -2.1888 Soluble
98 D E 0.0000 Buried
99 T E -0.6510 Soluble
100 A E 0.0000 Buried
101 V E 0.4694 Aggregation-prone
102 Y E 0.0000 Buried
103 Y E 0.0000 Buried
104 C E 0.0000 Buried
105 A E 0.0000 Buried
106 R E 0.0000 Buried
107 R E -0.3231 Soluble
108 T E -0.5972 Soluble
109 G E -1.4153 Soluble
113 R E -2.1011 Soluble
114 I E -0.2818 Soluble
115 F E 0.0000 Buried
116 D E -0.5884 Soluble
117 Y E -0.3828 Soluble
118 W E -0.5027 Soluble
119 G E 0.0000 Buried
120 Q E -1.2597 Soluble
121 G E 0.0000 Buried
122 T E 0.1264 Aggregation-prone
123 L E 0.2929 Aggregation-prone
124 V E 0.0000 Buried
125 T E 0.0000 Buried
126 V E 0.0000 Buried
127 S E -0.9075 Soluble
128 S E -0.6679 Soluble
129 A E -0.5255 Soluble
130 S E -0.7343 Soluble
131 T E -0.8523 Soluble
132 K E -1.5042 Soluble
133 G E -1.4204 Soluble
134 P E -0.8355 Soluble
135 S E -0.3558 Soluble
136 V E 0.0000 Buried
137 F E -0.7230 Soluble
138 P E 0.0000 Buried
139 L E 0.0000 Buried
140 A E -0.7923 Soluble
141 P E -0.3548 Soluble
142 C E -0.2660 Soluble
143 S E -1.1518 Soluble
144 R E -2.2474 Soluble
145 S E -1.5622 Soluble
146 T E -1.4493 Soluble
147 S E -1.4608 Soluble
148 E E -2.1225 Soluble
149 S E -1.2614 Soluble
150 T E -0.8465 Soluble
151 A E 0.0000 Buried
152 A E 0.0000 Buried
153 L E 0.0000 Buried
154 G E 0.0000 Buried
155 C E 0.0000 Buried
156 L E 0.0000 Buried
157 V E 0.0000 Buried
158 K E 0.0000 Buried
159 D E -0.3963 Soluble
160 Y E 0.0000 Buried
161 F E 0.0000 Buried
162 P E 0.0000 Buried
163 E E -0.4515 Soluble
164 P E -0.7374 Soluble
165 V E -0.5340 Soluble
166 T E -0.5772 Soluble
167 V E -0.2448 Soluble
168 S E -0.5101 Soluble
169 W E 0.0000 Buried
170 N E -0.9502 Soluble
171 S E -0.7799 Soluble
172 G E -0.4521 Soluble
173 A E -0.1886 Soluble
174 L E -0.0684 Soluble
175 T E -0.1952 Soluble
176 S E -0.2621 Soluble
177 G E -0.3932 Soluble
178 V E 0.0421 Aggregation-prone
179 H E -0.3811 Soluble
180 T E -0.0254 Soluble
181 F E 0.0000 Buried
182 P E -0.0983 Soluble
183 A E 0.4509 Aggregation-prone
184 V E 0.7317 Aggregation-prone
185 L E 1.5051 Aggregation-prone
186 Q E 0.5032 Aggregation-prone
187 S E -0.2609 Soluble
188 S E -0.1597 Soluble
189 G E 0.1469 Aggregation-prone
190 L E 0.1750 Aggregation-prone
191 Y E 0.5029 Aggregation-prone
192 S E 0.0000 Buried
193 L E 0.0000 Buried
194 S E 0.0000 Buried
195 S E 0.0000 Buried
196 V E 0.0000 Buried
197 V E 0.0000 Buried
198 T E -0.2325 Soluble
199 V E 0.0000 Buried
200 P E -0.5969 Soluble
201 S E -1.0919 Soluble
202 S E -0.7334 Soluble
203 S E -0.5475 Soluble
204 L E -1.0043 Soluble
205 G E -1.2598 Soluble
206 T E -0.7144 Soluble
207 Q E -1.0582 Soluble
208 T E -1.2641 Soluble
209 Y E 0.0000 Buried
210 T E -1.6215 Soluble
211 C E 0.0000 Buried
212 N E -1.6940 Soluble
213 V E 0.0000 Buried
214 D E -1.8023 Soluble
215 H E 0.0000 Buried
216 K E -2.6599 Soluble
217 P E -1.5198 Soluble
218 S E -1.8573 Soluble
219 N E -2.4341 Soluble
220 T E -2.1599 Soluble
221 K E -2.7384 Soluble
222 V E -1.7406 Soluble
223 D E -2.7219 Soluble
224 K E -2.2902 Soluble
225 R E -2.8828 Soluble
226 V E 0.0000 Buried
227 E E -2.5519 Soluble
1 E F -1.8128 Soluble
2 I F 0.0000 Buried
3 V F 0.6606 Aggregation-prone
4 L F 0.0000 Buried
5 T F -0.5483 Soluble
6 Q F 0.0000 Buried
7 S F -0.6194 Soluble
8 P F -0.3134 Soluble
9 A F -0.3411 Soluble
10 T F -0.3608 Soluble
11 L F -0.2746 Soluble
12 S F -0.6112 Soluble
13 V F -1.1022 Soluble
14 S F -1.8485 Soluble
15 P F -2.2961 Soluble
16 G F -2.5652 Soluble
17 E F -3.4832 Soluble
18 R F -3.4126 Soluble
19 A F 0.0000 Buried
20 T F -0.6335 Soluble
21 L F 0.0000 Buried
22 S F -0.7240 Soluble
23 C F 0.0000 Buried
24 R F -2.0121 Soluble
25 A F -1.1362 Soluble
26 S F -1.2332 Soluble
27 R F -2.0290 Soluble
28 S F -2.0179 Soluble
29 V F 0.0000 Buried
36 R F -3.1908 Soluble
37 R F -3.0057 Soluble
38 E F -1.8754 Soluble
39 L F 0.0000 Buried
40 A F 0.0000 Buried
41 W F 0.0000 Buried
42 Y F 0.0000 Buried
43 Q F 0.0000 Buried
44 Q F 0.0000 Buried
45 K F -2.0491 Soluble
46 P F -1.6838 Soluble
47 G F -1.7295 Soluble
48 Q F -2.2604 Soluble
49 A F -1.6152 Soluble
50 P F 0.0000 Buried
51 E F -2.0564 Soluble
52 L F 0.0000 Buried
53 L F 0.0000 Buried
54 I F 0.0000 Buried
55 Y F -0.9208 Soluble
56 G F -1.5903 Soluble
57 A F 0.0000 Buried
65 S F -1.1240 Soluble
66 T F -1.1396 Soluble
67 R F -2.1339 Soluble
68 E F -1.4501 Soluble
69 T F -0.8990 Soluble
70 G F -0.8015 Soluble
71 I F -0.8363 Soluble
72 P F -0.5665 Soluble
74 A F -0.6270 Soluble
75 R F -1.2235 Soluble
76 F F 0.0000 Buried
77 S F -0.8383 Soluble
78 G F -0.6673 Soluble
79 S F -0.7628 Soluble
80 G F -1.4234 Soluble
83 S F -1.7585 Soluble
84 G F -1.9706 Soluble
85 T F -2.3375 Soluble
86 D F -2.4068 Soluble
87 F F 0.0000 Buried
88 T F -0.7141 Soluble
89 L F 0.0000 Buried
90 T F 0.0000 Buried
91 I F 0.0000 Buried
92 N F -2.9986 Soluble
93 S F -2.5125 Soluble
94 L F 0.0000 Buried
95 E F -3.1997 Soluble
96 A F -2.9841 Soluble
97 E F -3.3273 Soluble
98 D F 0.0000 Buried
99 A F 0.0000 Buried
100 A F 0.0000 Buried
101 T F -0.7412 Soluble
102 Y F 0.0000 Buried
103 Y F 0.0000 Buried
104 C F 0.0000 Buried
105 Q F 0.0000 Buried
106 Q F 0.0000 Buried
107 Y F -0.7513 Soluble
108 R F 0.0000 Buried
109 D F -1.7107 Soluble
114 P F -1.0426 Soluble
115 P F -1.0876 Soluble
116 G F 0.0000 Buried
117 T F -0.3078 Soluble
118 F F 0.0000 Buried
119 G F 0.0000 Buried
120 G F -0.7324 Soluble
121 G F -0.9637 Soluble
122 T F 0.0000 Buried
123 K F -0.9886 Soluble
124 V F 0.0000 Buried
125 E F 0.0000 Buried
126 I F 0.0000 Buried
127 K F -1.4496 Soluble
128 R F -1.3186 Soluble
129 T F -0.1284 Soluble
130 V F 0.4968 Aggregation-prone
131 A F 0.1508 Aggregation-prone
132 A F -0.1246 Soluble
133 P F 0.0000 Buried
134 S F -0.2779 Soluble
135 V F 0.0000 Buried
136 F F -0.0178 Soluble
137 I F 0.0804 Aggregation-prone
138 F F 0.0000 Buried
139 P F 0.0000 Buried
140 P F 0.0000 Buried
141 S F -1.4768 Soluble
142 D F -2.9155 Soluble
143 E F -3.1386 Soluble
144 Q F 0.0000 Buried
145 L F -2.3858 Soluble
146 K F -2.9315 Soluble
147 S F -1.9635 Soluble
148 G F -1.5745 Soluble
149 T F -0.9730 Soluble
150 A F 0.0000 Buried
151 S F 0.0000 Buried
152 V F 0.0000 Buried
153 V F 0.0000 Buried
154 C F 0.0000 Buried
155 L F 0.0000 Buried
156 L F 0.0000 Buried
157 N F 0.0000 Buried
158 N F -1.2560 Soluble
159 F F 0.0000 Buried
160 Y F 0.0000 Buried
161 P F -1.5143 Soluble
162 R F -2.3743 Soluble
163 E F -2.8862 Soluble
164 A F -2.2049 Soluble
165 K F -2.4311 Soluble
166 V F -1.3162 Soluble
167 Q F -0.5220 Soluble
168 W F 0.0000 Buried
169 K F -0.7842 Soluble
170 V F 0.0000 Buried
171 D F -1.7099 Soluble
172 N F -1.5053 Soluble
173 A F -0.2696 Soluble
174 L F 0.6692 Aggregation-prone
175 Q F -0.4263 Soluble
176 S F -0.6421 Soluble
177 G F -1.0738 Soluble
178 N F -1.5072 Soluble
179 S F -1.3659 Soluble
180 Q F -1.2485 Soluble
181 E F -1.9399 Soluble
182 S F 0.0000 Buried
183 V F -0.6468 Soluble
184 T F -1.1935 Soluble
185 E F -2.0682 Soluble
186 Q F 0.0000 Buried
187 D F -2.4602 Soluble
188 S F -2.9087 Soluble
189 K F -3.4119 Soluble
190 D F -3.2309 Soluble
191 S F 0.0000 Buried
192 T F 0.0000 Buried
193 Y F 0.0000 Buried
194 S F 0.0000 Buried
195 L F 0.0000 Buried
196 S F 0.0000 Buried
197 S F 0.0000 Buried
198 T F 0.0000 Buried
199 L F 0.0000 Buried
200 T F -0.4960 Soluble
201 L F -0.6297 Soluble
202 S F -1.1872 Soluble
203 K F -2.1358 Soluble
204 A F -1.9555 Soluble
205 D F -2.1589 Soluble
206 Y F 0.0000 Buried
207 E F -3.0854 Soluble
208 K F -3.3112 Soluble
209 H F -2.8155 Soluble
210 K F -3.3125 Soluble
211 V F -1.4921 Soluble
212 Y F 0.0000 Buried
213 A F 0.0000 Buried
214 C F 0.0000 Buried
215 E F -0.7281 Soluble
216 V F 0.0000 Buried
217 T F -1.1996 Soluble
218 H F 0.0000 Buried
219 Q F -1.4389 Soluble
220 G F -0.3702 Soluble
221 L F -0.3001 Soluble
222 S F -0.5316 Soluble
223 S F -0.4406 Soluble
224 P F -0.5692 Soluble
225 V F -0.0653 Soluble
226 T F -0.5245 Soluble
227 K F -0.8552 Soluble
228 S F -0.6747 Soluble
229 F F -0.9349 Soluble
230 N F -2.1214 Soluble
231 R F -2.4478 Soluble
232 G F -2.0682 Soluble
233 E F -2.2163 Soluble
234 C F -0.5986 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.8116 4.0374 CSV PDB
4.5 -0.8569 4.0374 CSV PDB
5.0 -0.9118 4.0374 CSV PDB
5.5 -0.9645 4.0374 CSV PDB
6.0 -1.0029 4.0374 CSV PDB
6.5 -1.018 4.0374 CSV PDB
7.0 -1.0101 4.0374 CSV PDB
7.5 -0.9868 4.0374 CSV PDB
8.0 -0.9558 4.0374 CSV PDB
8.5 -0.9199 4.0372 CSV PDB
9.0 -0.8792 4.0366 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH