Paridiprubart / 4r7d

A4DAB203_4r7d_E_F | Source PDB: 4r7d | Chains: E, F | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6495
Max Score
1.802
Min Score
-3.9209
Total Score
-272.79
Score CDR
-0.1157
Score non-CDR
-0.7136

Calculation Parameters

Chain Sequence(s) VQLQESGPGLVKPSDTLSLTCAVSGYSITGGYSWHWIRQPPGKGLEWMGYIHYSGYTDFNPSLKTRITISRDTSKNQFSLKLSSVTAVDTAVYYCARKDPSDAFPYWGQGTLVTVSSASTKGPSVFPLAPTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPIVLTQSPDFQSVTPKEKVTITCRASQSISDHLHWYQQKPDQSPKLLIKYASHAISGVPSRFSGSGSGTDFTLTINSLEAEDAATYYCQQGHSFPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG input.pdb
Selected Chain(s) E (Heavy), F (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.9209
Maximum Score 1.802
Average Score -0.6495
Total Score -272.7917

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V E 0.9034 Aggregation-prone
3 Q E -0.7328 Soluble
4 L E 0.0000 Buried
5 Q E -1.5493 Soluble
6 E E -1.0158 Soluble
7 S E -0.8326 Soluble
8 G E -0.5330 Soluble
9 P E -0.3320 Soluble
11 G E -0.4823 Soluble
12 L E -0.2816 Soluble
13 V E 0.0000 Buried
14 K E -2.2053 Soluble
15 P E -1.5768 Soluble
16 S E -1.6520 Soluble
17 D E -2.4730 Soluble
18 T E -1.6877 Soluble
19 L E 0.0000 Buried
20 S E -1.0357 Soluble
21 L E 0.0000 Buried
22 T E -0.7781 Soluble
23 C E 0.0000 Buried
24 A E -0.9882 Soluble
25 V E 0.0000 Buried
26 S E -0.5693 Soluble
27 G E -0.0515 Soluble
28 Y E 0.3175 Aggregation-prone
29 S E -0.0901 Soluble
30 I E 0.0000 Buried
31 T E -0.1079 Soluble
35 G E -0.2094 Soluble
36 G E -0.2008 Soluble
37 Y E 0.0696 Aggregation-prone
38 S E 0.0000 Buried
39 W E 0.0000 Buried
40 H E 0.0000 Buried
41 W E 0.0000 Buried
42 I E 0.0000 Buried
43 R E 0.0000 Buried
44 Q E -0.7618 Soluble
45 P E -0.5955 Soluble
46 P E -1.1677 Soluble
47 G E -1.4777 Soluble
48 K E -2.2849 Soluble
49 G E -1.4642 Soluble
50 L E 0.0000 Buried
51 E E -0.8683 Soluble
52 W E 0.0000 Buried
53 M E 0.0000 Buried
54 G E 0.0000 Buried
55 Y E -0.0573 Soluble
56 I E 0.0000 Buried
57 H E 0.2387 Aggregation-prone
58 Y E 0.5844 Aggregation-prone
59 S E 0.2514 Aggregation-prone
63 G E 0.3480 Aggregation-prone
64 Y E 0.8561 Aggregation-prone
65 T E -0.0435 Soluble
66 D E -1.1439 Soluble
67 F E -0.9369 Soluble
68 N E -0.8290 Soluble
69 P E -1.1054 Soluble
70 S E -0.9203 Soluble
71 L E 0.0000 Buried
72 K E -2.1766 Soluble
74 T E -1.1574 Soluble
75 R E -1.2095 Soluble
76 I E 0.0000 Buried
77 T E -1.0915 Soluble
78 I E 0.0000 Buried
79 S E -0.7221 Soluble
80 R E -1.3182 Soluble
81 D E -1.6021 Soluble
82 T E -1.2911 Soluble
83 S E -1.4402 Soluble
84 K E -2.2548 Soluble
85 N E -1.5173 Soluble
86 Q E -1.4403 Soluble
87 F E 0.0000 Buried
88 S E -0.8327 Soluble
89 L E 0.0000 Buried
90 K E -1.6027 Soluble
91 L E 0.0000 Buried
92 S E -1.1447 Soluble
93 S E -1.0787 Soluble
94 V E 0.0000 Buried
95 T E 0.0295 Aggregation-prone
96 A E 0.9203 Aggregation-prone
97 V E 1.8020 Aggregation-prone
98 D E 0.0000 Buried
99 T E 0.6334 Aggregation-prone
100 A E 0.0000 Buried
101 V E -0.0539 Soluble
102 Y E 0.0000 Buried
103 Y E 0.0000 Buried
104 C E 0.0000 Buried
105 A E 0.0000 Buried
106 R E 0.0000 Buried
107 K E -0.2460 Soluble
108 D E -0.4539 Soluble
109 P E -0.4906 Soluble
110 S E -0.4476 Soluble
113 D E -0.6653 Soluble
114 A E 0.0000 Buried
115 F E 0.0000 Buried
116 P E -0.1224 Soluble
117 Y E 0.3640 Aggregation-prone
118 W E -0.4576 Soluble
119 G E 0.0000 Buried
120 Q E -2.0747 Soluble
121 G E -1.2463 Soluble
122 T E -0.4642 Soluble
123 L E -0.0642 Soluble
124 V E 0.0000 Buried
125 T E 0.0169 Aggregation-prone
126 V E -0.3116 Soluble
127 S E -0.5583 Soluble
128 S E -0.7729 Soluble
129 A E -0.5472 Soluble
130 S E -0.7489 Soluble
131 T E -0.8915 Soluble
132 K E -1.7519 Soluble
133 G E -1.6533 Soluble
134 P E -0.6756 Soluble
135 S E -0.3604 Soluble
136 V E -0.2938 Soluble
137 F E -0.4964 Soluble
138 P E -1.0434 Soluble
139 L E 0.0000 Buried
140 A E -0.9430 Soluble
141 P E -0.5950 Soluble
142 T E -0.2808 Soluble
143 A E -0.2010 Soluble
144 A E 0.0000 Buried
145 L E 0.0000 Buried
146 G E 0.0000 Buried
147 C E 0.0000 Buried
148 L E 0.0000 Buried
149 V E 0.0000 Buried
150 K E -0.2265 Soluble
151 D E -0.4054 Soluble
152 Y E 0.0000 Buried
153 F E 0.0000 Buried
154 P E 0.0000 Buried
155 E E -0.8385 Soluble
156 P E -0.7550 Soluble
157 V E -0.4895 Soluble
158 T E -0.5562 Soluble
159 V E -0.1778 Soluble
160 S E -0.3913 Soluble
161 W E 0.0000 Buried
162 N E -0.7382 Soluble
163 S E -0.6150 Soluble
164 G E -0.3467 Soluble
165 A E -0.1564 Soluble
166 L E 0.0312 Aggregation-prone
167 T E -0.1302 Soluble
168 S E -0.1718 Soluble
169 G E -0.2175 Soluble
170 V E 0.1929 Aggregation-prone
171 H E -0.2617 Soluble
172 T E 0.0627 Aggregation-prone
173 F E 0.0000 Buried
174 P E -0.1223 Soluble
175 A E 0.2748 Aggregation-prone
176 V E 0.6436 Aggregation-prone
177 L E 1.5116 Aggregation-prone
178 Q E 0.4104 Aggregation-prone
179 S E -0.0313 Soluble
180 S E -0.1815 Soluble
181 G E 0.1040 Aggregation-prone
182 L E 0.0391 Aggregation-prone
183 Y E 0.4312 Aggregation-prone
184 S E 0.0000 Buried
185 L E 0.0000 Buried
186 S E 0.0000 Buried
187 S E 0.0000 Buried
188 V E 0.0000 Buried
189 V E 0.0000 Buried
190 T E -0.1605 Soluble
191 V E 0.0000 Buried
192 P E -0.5398 Soluble
193 S E -0.4931 Soluble
194 S E -0.5751 Soluble
195 S E -0.6687 Soluble
196 L E -0.8683 Soluble
197 G E -1.0356 Soluble
198 T E -0.7750 Soluble
199 Q E -1.1951 Soluble
200 T E -1.1970 Soluble
201 Y E 0.0000 Buried
202 I E -1.3957 Soluble
203 C E 0.0000 Buried
204 N E -1.4973 Soluble
205 V E 0.0000 Buried
206 N E -1.9265 Soluble
207 H E 0.0000 Buried
208 K E -2.7906 Soluble
209 P E -1.7371 Soluble
210 S E -1.9584 Soluble
211 N E -2.6685 Soluble
212 T E -2.1337 Soluble
213 K E -2.6230 Soluble
214 V E -1.3558 Soluble
215 D E -2.5807 Soluble
216 K E -2.1859 Soluble
217 R E -2.9139 Soluble
218 V E 0.0000 Buried
219 E E -2.5402 Soluble
220 P E -1.4386 Soluble
2 I F 1.2756 Aggregation-prone
3 V F 1.4919 Aggregation-prone
4 L F 0.0000 Buried
5 T F -0.8393 Soluble
6 Q F 0.0000 Buried
7 S F -0.9485 Soluble
8 P F -0.8779 Soluble
9 D F -1.5645 Soluble
10 F F -0.0384 Soluble
11 Q F -0.3816 Soluble
12 S F -0.5386 Soluble
13 V F 0.0000 Buried
14 T F -2.1623 Soluble
15 P F -2.4458 Soluble
16 K F -3.4090 Soluble
17 E F -3.6705 Soluble
18 K F -3.3109 Soluble
19 V F 0.0000 Buried
20 T F -0.7283 Soluble
21 I F 0.0000 Buried
22 T F -0.7690 Soluble
23 C F 0.0000 Buried
24 R F -2.2346 Soluble
25 A F 0.0000 Buried
26 S F -0.5986 Soluble
27 Q F -1.3514 Soluble
28 S F -1.4901 Soluble
29 I F 0.0000 Buried
36 S F -1.1135 Soluble
37 D F -1.2000 Soluble
38 H F -1.0033 Soluble
39 L F 0.0000 Buried
40 H F 0.0000 Buried
41 W F 0.0000 Buried
42 Y F 0.0000 Buried
43 Q F 0.0000 Buried
44 Q F 0.0000 Buried
45 K F -2.1056 Soluble
46 P F -1.8135 Soluble
47 D F -2.7472 Soluble
48 Q F -2.7888 Soluble
49 S F -1.7186 Soluble
50 P F 0.0000 Buried
51 K F -1.5024 Soluble
52 L F 0.0000 Buried
53 L F 0.0000 Buried
54 I F 0.0000 Buried
55 K F -0.3800 Soluble
56 Y F -0.0644 Soluble
57 A F 0.0000 Buried
65 S F -0.6731 Soluble
66 H F -0.8304 Soluble
67 A F -0.3227 Soluble
68 I F -0.0688 Soluble
69 S F -0.2222 Soluble
70 G F -0.3622 Soluble
71 V F -0.1280 Soluble
72 P F -0.3105 Soluble
74 S F -0.6786 Soluble
75 R F -1.5155 Soluble
76 F F 0.0000 Buried
77 S F -0.7714 Soluble
78 G F -0.4349 Soluble
79 S F -0.7137 Soluble
80 G F -0.9811 Soluble
83 S F -1.1594 Soluble
84 G F -1.3938 Soluble
85 T F -1.7042 Soluble
86 D F -2.0571 Soluble
87 F F 0.0000 Buried
88 T F -0.7094 Soluble
89 L F 0.0000 Buried
90 T F -0.8906 Soluble
91 I F 0.0000 Buried
92 N F -2.8172 Soluble
93 S F -2.8328 Soluble
94 L F 0.0000 Buried
95 E F -2.8508 Soluble
96 A F -2.6856 Soluble
97 E F -2.9074 Soluble
98 D F 0.0000 Buried
99 A F 0.0000 Buried
100 A F 0.0000 Buried
101 T F -0.5503 Soluble
102 Y F 0.0000 Buried
103 Y F 0.0000 Buried
104 C F 0.0000 Buried
105 Q F 0.0000 Buried
106 Q F 0.0000 Buried
107 G F 0.0000 Buried
108 H F -0.8442 Soluble
109 S F 0.2310 Aggregation-prone
114 F F 1.2952 Aggregation-prone
115 P F 0.6119 Aggregation-prone
116 L F 0.0000 Buried
117 T F 0.4940 Aggregation-prone
118 F F 0.0000 Buried
119 G F 0.0000 Buried
120 G F -1.0656 Soluble
121 G F -1.0421 Soluble
122 T F 0.0000 Buried
123 K F -0.6741 Soluble
124 V F 0.0000 Buried
125 E F 0.0000 Buried
126 I F 0.0000 Buried
127 K F -1.6634 Soluble
128 R F -0.8223 Soluble
129 T F 0.1673 Aggregation-prone
130 V F 1.1008 Aggregation-prone
131 A F 0.3836 Aggregation-prone
132 A F 0.0859 Aggregation-prone
133 P F 0.0000 Buried
134 S F -0.0602 Soluble
135 V F 0.1038 Aggregation-prone
136 F F 0.2205 Aggregation-prone
137 I F 0.1468 Aggregation-prone
138 F F 0.0000 Buried
139 P F -0.6596 Soluble
140 P F -0.7588 Soluble
141 S F -1.3763 Soluble
142 D F -2.6325 Soluble
143 E F -2.3669 Soluble
144 Q F 0.0000 Buried
145 L F -1.8874 Soluble
146 K F -2.6334 Soluble
147 S F -1.5794 Soluble
148 G F -1.3482 Soluble
149 T F -1.0018 Soluble
150 A F 0.0000 Buried
151 S F 0.0000 Buried
152 V F 0.0000 Buried
153 V F 0.0000 Buried
154 C F 0.0000 Buried
155 L F 0.0000 Buried
156 L F 0.0000 Buried
157 N F 0.0000 Buried
158 N F -0.8782 Soluble
159 F F 0.0000 Buried
160 Y F 0.0000 Buried
161 P F -1.2522 Soluble
162 R F -2.0946 Soluble
163 E F -2.8817 Soluble
164 A F -2.1483 Soluble
165 K F -2.3278 Soluble
166 V F -1.0359 Soluble
167 Q F -0.5450 Soluble
168 W F 0.0000 Buried
169 K F -0.4762 Soluble
170 V F 0.0000 Buried
171 D F -1.8279 Soluble
172 N F -1.3142 Soluble
173 A F -0.2529 Soluble
174 L F 0.7852 Aggregation-prone
175 Q F -0.0962 Soluble
176 S F -0.3677 Soluble
177 G F -0.8422 Soluble
178 N F -0.7455 Soluble
179 S F -1.0215 Soluble
180 Q F -1.1381 Soluble
181 E F -1.7415 Soluble
182 S F 0.0000 Buried
183 V F -0.1280 Soluble
184 T F -0.6586 Soluble
185 E F -1.1660 Soluble
186 Q F 0.0000 Buried
187 D F -2.0279 Soluble
188 S F -2.4251 Soluble
189 K F -2.8559 Soluble
190 D F -2.0608 Soluble
191 S F 0.0000 Buried
192 T F 0.0000 Buried
193 Y F 0.0000 Buried
194 S F 0.0000 Buried
195 L F 0.0000 Buried
196 S F 0.0000 Buried
197 S F 0.0000 Buried
198 T F -0.6341 Soluble
199 L F 0.0000 Buried
200 T F -0.4805 Soluble
201 L F -0.9545 Soluble
202 S F -1.3627 Soluble
203 K F -2.3938 Soluble
204 A F -2.2903 Soluble
205 D F -3.1121 Soluble
206 Y F 0.0000 Buried
207 E F -3.9209 Soluble
208 K F -3.7419 Soluble
209 H F -3.1059 Soluble
210 K F -3.1274 Soluble
211 V F -1.8335 Soluble
212 Y F 0.0000 Buried
213 A F 0.0000 Buried
214 C F 0.0000 Buried
215 E F -0.7817 Soluble
216 V F 0.0000 Buried
217 T F -1.1809 Soluble
218 H F 0.0000 Buried
219 Q F -1.5480 Soluble
220 G F -0.2474 Soluble
221 L F -0.1434 Soluble
222 S F -0.4364 Soluble
223 S F -0.3994 Soluble
224 P F -0.5239 Soluble
225 V F -0.0374 Soluble
226 T F -0.4724 Soluble
227 K F -0.6748 Soluble
228 S F -0.7633 Soluble
229 F F -0.9588 Soluble
230 N F -2.1120 Soluble
231 R F -2.4737 Soluble
232 G F -1.5163 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6451 3.6822 CSV PDB
4.5 -0.6881 3.6605 CSV PDB
5.0 -0.7378 3.6354 CSV PDB
5.5 -0.7832 3.6158 CSV PDB
6.0 -0.8125 3.6089 CSV PDB
6.5 -0.8178 3.6105 CSV PDB
7.0 -0.8004 3.6056 CSV PDB
7.5 -0.7692 3.5868 CSV PDB
8.0 -0.7305 3.5586 CSV PDB
8.5 -0.6858 3.5279 CSV PDB
9.0 -0.6339 3.5006 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH