Casdozokitug / 7zxk

A4DAB059_7zxk_J_I | Source PDB: 7zxk | Chains: J, I | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6987
Max Score
1.8367
Min Score
-3.4717
Total Score
-311.61
Score CDR
-0.2757
Score non-CDR
-0.7619

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVKPGGSLRLSCAASGFTFRSYGMNWVRQAPGKGLEWVSGISSSGSYIYYADSVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCARDGGRTSYTATAHNWFDPWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDIVMTQSPDSLAVSLGERATINCKSSQSVLFSSNKNYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQHASAPPTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) J (Heavy), I (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.4717
Maximum Score 1.8367
Average Score -0.6987
Total Score -311.6144

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E J -1.7148 Soluble
2 V J -0.3827 Soluble
3 Q J -0.9024 Soluble
4 L J 0.0000 Buried
5 V J 1.2188 Aggregation-prone
6 E J 0.0000 Buried
7 S J -0.2983 Soluble
8 G J -0.8663 Soluble
9 G J -0.4551 Soluble
11 G J -0.2490 Soluble
12 L J -0.2093 Soluble
13 V J 0.0000 Buried
14 K J -2.0938 Soluble
15 P J -1.8078 Soluble
16 G J -1.4709 Soluble
17 G J -1.2523 Soluble
18 S J -1.1974 Soluble
19 L J -1.1988 Soluble
20 R J -2.0034 Soluble
21 L J 0.0000 Buried
22 S J -0.2733 Soluble
23 C J 0.0000 Buried
24 A J -0.1157 Soluble
25 A J 0.0000 Buried
26 S J -0.6686 Soluble
27 G J -0.7986 Soluble
28 F J -0.4632 Soluble
29 T J -0.7299 Soluble
30 F J 0.0000 Buried
35 R J -2.2074 Soluble
36 S J -1.3980 Soluble
37 Y J -1.0333 Soluble
38 G J -0.4885 Soluble
39 M J 0.0000 Buried
40 N J 0.0000 Buried
41 W J 0.0000 Buried
42 V J 0.0000 Buried
43 R J 0.0000 Buried
44 Q J -0.8949 Soluble
45 A J -1.2494 Soluble
46 P J -0.9686 Soluble
47 G J -1.4689 Soluble
48 K J -2.3153 Soluble
49 G J -1.4817 Soluble
50 L J 0.0000 Buried
51 E J -1.2915 Soluble
52 W J 0.0000 Buried
53 V J 0.0000 Buried
54 S J 0.0000 Buried
55 G J 0.0000 Buried
56 I J 0.0000 Buried
57 S J 0.0000 Buried
58 S J -0.8847 Soluble
59 S J -0.5775 Soluble
62 G J -0.1172 Soluble
63 S J 0.5362 Aggregation-prone
64 Y J 1.7425 Aggregation-prone
65 I J 1.8367 Aggregation-prone
66 Y J 1.0269 Aggregation-prone
67 Y J -0.3270 Soluble
68 A J -1.2535 Soluble
69 D J -2.3757 Soluble
70 S J -1.7767 Soluble
71 V J 0.0000 Buried
72 K J -2.5244 Soluble
74 G J -1.7463 Soluble
75 R J -1.4018 Soluble
76 F J 0.0000 Buried
77 T J -0.5908 Soluble
78 I J 0.0000 Buried
79 S J -0.1983 Soluble
80 R J -1.1334 Soluble
81 D J -1.7318 Soluble
82 N J -2.1567 Soluble
83 A J -1.6038 Soluble
84 K J -2.2535 Soluble
85 N J -1.9312 Soluble
86 S J 0.0000 Buried
87 L J 0.0000 Buried
88 Y J -0.3340 Soluble
89 L J 0.0000 Buried
90 Q J -1.0642 Soluble
91 M J 0.0000 Buried
92 N J -1.3139 Soluble
93 S J -1.1893 Soluble
94 L J 0.0000 Buried
95 R J -2.0938 Soluble
96 A J -1.6664 Soluble
97 E J -2.1472 Soluble
98 D J 0.0000 Buried
99 T J -0.6961 Soluble
100 A J 0.0000 Buried
101 V J 0.1943 Aggregation-prone
102 Y J 0.0000 Buried
103 Y J 0.0000 Buried
104 C J 0.0000 Buried
105 A J 0.0000 Buried
106 R J -0.3329 Soluble
107 D J 0.0000 Buried
108 G J -0.6671 Soluble
109 G J -0.9577 Soluble
110 R J -1.8134 Soluble
111 T J -0.7996 Soluble
111A S J -0.2330 Soluble
111B Y J 0.2936 Aggregation-prone
111C T J 0.0000 Buried
112C A J -0.0987 Soluble
112B T J 0.0000 Buried
112A A J 0.0000 Buried
112 H J -0.1457 Soluble
113 N J 0.0000 Buried
114 W J 0.0000 Buried
115 F J 0.0000 Buried
116 D J -0.7222 Soluble
117 P J -0.7186 Soluble
118 W J 0.0000 Buried
119 G J 0.0000 Buried
120 Q J -1.2300 Soluble
121 G J 0.0000 Buried
122 T J 0.0306 Aggregation-prone
123 L J 0.0453 Aggregation-prone
124 V J 0.0000 Buried
125 T J -0.5289 Soluble
126 V J 0.0000 Buried
127 S J -1.1104 Soluble
128 S J -0.9282 Soluble
129 A J -0.6487 Soluble
130 S J -0.7761 Soluble
131 T J -0.8907 Soluble
132 K J -1.7795 Soluble
133 G J -1.8379 Soluble
134 P J -0.8821 Soluble
135 S J -0.5481 Soluble
136 V J 0.0000 Buried
137 F J 0.0000 Buried
138 P J -1.0974 Soluble
139 L J 0.0000 Buried
140 A J -0.9319 Soluble
141 P J 0.0000 Buried
142 S J -1.0103 Soluble
143 S J -1.2635 Soluble
144 K J -1.9007 Soluble
145 S J -1.2330 Soluble
146 T J -0.7689 Soluble
147 S J -0.7259 Soluble
148 G J -0.7549 Soluble
149 G J -0.9658 Soluble
150 T J -0.7470 Soluble
151 A J 0.0000 Buried
152 A J 0.0000 Buried
153 L J 0.0000 Buried
154 G J 0.0000 Buried
155 C J 0.0000 Buried
156 L J 0.0000 Buried
157 V J 0.0000 Buried
158 K J 0.0000 Buried
159 D J -0.6256 Soluble
160 Y J 0.0000 Buried
161 F J 0.0000 Buried
162 P J 0.0000 Buried
163 E J -0.6487 Soluble
164 P J -0.7375 Soluble
165 V J -0.7451 Soluble
166 T J -0.6522 Soluble
167 V J -0.2231 Soluble
168 S J -0.4026 Soluble
169 W J 0.0000 Buried
170 N J -0.6858 Soluble
171 S J -0.6274 Soluble
172 G J -0.4697 Soluble
173 A J -0.2151 Soluble
174 L J 0.0420 Aggregation-prone
175 T J -0.1505 Soluble
176 S J -0.1729 Soluble
177 G J -0.2066 Soluble
178 V J 0.1382 Aggregation-prone
179 H J -0.3071 Soluble
180 T J 0.0208 Aggregation-prone
181 F J 0.0000 Buried
182 P J -0.2355 Soluble
183 A J 0.2969 Aggregation-prone
184 V J 0.6585 Aggregation-prone
185 L J 1.5087 Aggregation-prone
186 Q J 0.4223 Aggregation-prone
187 S J -0.1119 Soluble
188 S J -0.2211 Soluble
189 G J 0.0178 Aggregation-prone
190 L J -0.0266 Soluble
191 Y J 0.4362 Aggregation-prone
192 S J 0.0000 Buried
193 L J 0.2353 Aggregation-prone
195 S J 0.0000 Buried
196 V J 0.0000 Buried
197 V J 0.0000 Buried
198 T J -0.1551 Soluble
199 V J 0.0000 Buried
200 P J -0.5708 Soluble
201 S J -0.7483 Soluble
202 S J -0.5755 Soluble
204 L J -0.8389 Soluble
205 G J -0.9889 Soluble
206 T J -0.7800 Soluble
207 Q J -1.1459 Soluble
208 T J -1.1080 Soluble
209 Y J 0.0000 Buried
210 I J -1.1190 Soluble
211 C J 0.0000 Buried
212 N J -1.4044 Soluble
213 V J 0.0000 Buried
214 N J -2.2105 Soluble
215 H J 0.0000 Buried
216 K J -2.8530 Soluble
217 P J -1.6796 Soluble
218 S J -2.0486 Soluble
219 N J -2.6712 Soluble
220 T J -2.2267 Soluble
221 K J -2.7837 Soluble
222 V J -1.5992 Soluble
223 D J -2.3917 Soluble
224 K J -1.9618 Soluble
225 K J -2.3237 Soluble
226 V J 0.0000 Buried
227 E J -2.4760 Soluble
228 P J -1.5518 Soluble
229 K J -0.9817 Soluble
230 S J -0.3935 Soluble
231 C J 0.4270 Aggregation-prone
1 D I -1.5366 Soluble
2 I I 0.0000 Buried
3 V I 0.8379 Aggregation-prone
4 M I 0.0000 Buried
5 T I -0.6448 Soluble
6 Q I -1.2062 Soluble
7 S I -1.2595 Soluble
8 P I -1.4335 Soluble
9 D I -2.3315 Soluble
10 S I -1.4611 Soluble
11 L I -0.7665 Soluble
12 A I -0.5558 Soluble
13 V I -0.8435 Soluble
14 S I -0.8937 Soluble
15 L I -0.0369 Soluble
16 G I -0.8341 Soluble
17 E I -2.2918 Soluble
18 R I -2.5708 Soluble
19 A I 0.0000 Buried
20 T I -0.6595 Soluble
21 I I 0.0000 Buried
22 N I -1.6008 Soluble
23 C I 0.0000 Buried
24 K I -1.8151 Soluble
25 S I 0.0000 Buried
26 S I -0.9156 Soluble
27 Q I -1.5647 Soluble
28 S I -0.9289 Soluble
29 V I 0.0000 Buried
30 L I 0.4378 Aggregation-prone
31 F I 0.6397 Aggregation-prone
32 S I -0.0413 Soluble
33 S I -0.1688 Soluble
34 N I -0.3183 Soluble
36 K I -0.4133 Soluble
37 N I -0.1535 Soluble
38 Y I 0.0000 Buried
39 L I 0.0000 Buried
40 A I 0.0000 Buried
41 W I 0.0000 Buried
42 Y I 0.0000 Buried
43 Q I 0.0000 Buried
44 Q I 0.0000 Buried
45 K I -1.7691 Soluble
46 P I -1.3650 Soluble
47 G I -1.5249 Soluble
48 Q I -2.0230 Soluble
49 P I -1.4862 Soluble
50 P I 0.0000 Buried
51 K I -1.7045 Soluble
52 L I 0.0000 Buried
53 L I 0.0000 Buried
54 I I 0.0000 Buried
55 Y I -0.1925 Soluble
56 W I -0.1217 Soluble
57 A I 0.0000 Buried
65 S I -0.7066 Soluble
66 T I -0.6657 Soluble
67 R I -1.7148 Soluble
68 E I -1.4562 Soluble
69 S I -1.0817 Soluble
70 G I -1.0858 Soluble
71 V I 0.0000 Buried
72 P I -1.4911 Soluble
74 D I -2.3982 Soluble
75 R I -1.7328 Soluble
76 F I 0.0000 Buried
77 S I -1.0940 Soluble
78 G I -0.5831 Soluble
79 S I -0.8417 Soluble
80 G I -1.2840 Soluble
83 S I -1.0284 Soluble
84 G I -0.9102 Soluble
85 T I -1.6272 Soluble
86 D I -2.6624 Soluble
87 F I 0.0000 Buried
88 T I -1.0640 Soluble
89 L I 0.0000 Buried
90 T I -0.7182 Soluble
91 I I 0.0000 Buried
92 S I -1.7244 Soluble
93 S I -1.4700 Soluble
94 L I 0.0000 Buried
95 Q I -1.2701 Soluble
96 A I -1.7228 Soluble
97 E I -2.5260 Soluble
98 D I 0.0000 Buried
99 V I 0.0000 Buried
100 A I 0.0000 Buried
101 V I -0.6940 Soluble
102 Y I 0.0000 Buried
103 Y I 0.0000 Buried
104 C I 0.0000 Buried
105 Q I 0.0000 Buried
106 Q I 0.0000 Buried
107 H I 0.0000 Buried
108 A I 0.0000 Buried
109 S I -0.6774 Soluble
114 A I -0.3642 Soluble
115 P I -0.7092 Soluble
116 P I 0.0000 Buried
117 T I -0.1192 Soluble
118 F I 0.0972 Aggregation-prone
119 G I 0.0000 Buried
120 G I -1.1260 Soluble
121 G I -1.1631 Soluble
122 T I 0.0000 Buried
123 K I -1.5136 Soluble
124 V I 0.0000 Buried
125 E I -1.3043 Soluble
126 I I 0.0000 Buried
127 K I -1.1959 Soluble
128 R I -0.8465 Soluble
129 T I -0.1520 Soluble
130 V I 0.3089 Aggregation-prone
131 A I -0.0566 Soluble
132 A I -0.1033 Soluble
133 P I 0.0000 Buried
134 S I -0.1354 Soluble
135 V I 0.0000 Buried
136 F I -0.0972 Soluble
137 I I 0.1076 Aggregation-prone
138 F I 0.0000 Buried
139 P I -0.4744 Soluble
140 P I 0.0000 Buried
141 S I -1.6770 Soluble
142 D I -2.9556 Soluble
143 E I -2.8947 Soluble
144 Q I 0.0000 Buried
145 L I -2.2872 Soluble
146 K I -2.9066 Soluble
147 S I -1.9080 Soluble
148 G I -1.6560 Soluble
149 T I -1.1186 Soluble
150 A I 0.0000 Buried
151 S I 0.0000 Buried
152 V I 0.0000 Buried
153 V I 0.0000 Buried
154 C I 0.0000 Buried
155 L I 0.0000 Buried
156 L I 0.0000 Buried
157 N I -0.3262 Soluble
158 N I -0.8450 Soluble
159 F I 0.0000 Buried
160 Y I 0.0000 Buried
161 P I -1.6747 Soluble
162 R I -2.8223 Soluble
163 E I -3.0896 Soluble
164 A I -2.1130 Soluble
165 K I -1.9881 Soluble
166 V I -0.7997 Soluble
167 Q I -0.3955 Soluble
168 W I 0.0000 Buried
169 K I -0.5175 Soluble
170 V I 0.0000 Buried
171 D I -1.7994 Soluble
172 N I -1.5420 Soluble
173 A I -0.2982 Soluble
174 L I 0.7624 Aggregation-prone
175 Q I -0.2601 Soluble
176 S I -0.6164 Soluble
177 G I -1.2001 Soluble
178 N I -1.4660 Soluble
179 S I -1.3485 Soluble
180 Q I -1.2982 Soluble
181 E I -1.8378 Soluble
182 S I -0.9419 Soluble
183 V I -0.7614 Soluble
184 T I -0.9709 Soluble
185 E I -1.9203 Soluble
186 Q I 0.0000 Buried
187 D I -2.0518 Soluble
188 S I -2.3998 Soluble
189 K I -2.5776 Soluble
190 D I -1.7896 Soluble
191 S I 0.0000 Buried
192 T I 0.0000 Buried
193 Y I 0.0000 Buried
194 S I 0.0000 Buried
195 L I 0.0000 Buried
196 S I -0.5367 Soluble
197 S I 0.0000 Buried
198 T I -0.6748 Soluble
199 L I 0.0000 Buried
200 T I -0.5951 Soluble
201 L I -0.7330 Soluble
202 S I -1.2304 Soluble
203 K I -2.0182 Soluble
204 A I -1.8476 Soluble
205 D I -2.4847 Soluble
206 Y I 0.0000 Buried
207 E I -3.2714 Soluble
208 K I -3.4717 Soluble
209 H I -3.0413 Soluble
210 K I -3.4490 Soluble
211 V I -1.9047 Soluble
212 Y I 0.0000 Buried
213 A I 0.0000 Buried
214 C I 0.0000 Buried
215 E I -0.7351 Soluble
216 V I 0.0000 Buried
217 T I -1.0755 Soluble
218 H I 0.0000 Buried
219 Q I -1.7159 Soluble
220 G I -0.4398 Soluble
221 L I -0.2731 Soluble
222 S I -0.4800 Soluble
223 S I -0.4560 Soluble
224 P I -0.5742 Soluble
225 V I -0.0802 Soluble
226 T I -0.4457 Soluble
227 K I -0.6583 Soluble
228 S I -0.6061 Soluble
229 F I 0.0000 Buried
230 N I -2.3151 Soluble
231 R I -2.7893 Soluble
232 G I -2.1599 Soluble
233 E I -2.2270 Soluble
234 C I -0.4251 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7382 1.998 CSV PDB
4.5 -0.7843 1.998 CSV PDB
5.0 -0.8378 1.998 CSV PDB
5.5 -0.8877 1.998 CSV PDB
6.0 -0.9221 1.998 CSV PDB
6.5 -0.9322 1.998 CSV PDB
7.0 -0.9189 1.998 CSV PDB
7.5 -0.8904 1.9979 CSV PDB
8.0 -0.8536 1.9975 CSV PDB
8.5 -0.8102 1.9962 CSV PDB
9.0 -0.7599 1.9923 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH