Paridiprubart / 4r7n

A4DAB203_4r7n_M_N | Source PDB: 4r7n | Chains: M, N | Done
96
Yes
10 Å
Yes
Avg A4D Score
-0.6931
Max Score
1.7739
Min Score
-4.0917
Total Score
-297.35
Score CDR
-0.2115
Score non-CDR
-0.7496

Calculation Parameters

Chain Sequence(s) QVQLQESGPGLVKPSDTLSLTCAVSGYPIRFGYSWHWIRQPPGKGLEWMGYIHYSGYTDFNPSLKTRITISRDTSKNQFSLKLSSVTAVDTAVYYCARKDSGNYFPYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPEIVLTQSPDFQSVTPKEKVTITCRASQSISDHLHWYQQKPDQSPKLLIKYASHAISGVPSRFSGSGSGTDFTLTINSLEAEDAATYYCQQGYDFPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNR input.pdb
Selected Chain(s) M (Heavy), N (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -4.0917
Maximum Score 1.7739
Average Score -0.6931
Total Score -297.3512

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q M -1.5139 Soluble
2 V M 0.0000 Buried
3 Q M -1.8337 Soluble
4 L M 0.0000 Buried
5 Q M -1.5873 Soluble
6 E M 0.0000 Buried
7 S M -0.8094 Soluble
8 G M -0.8004 Soluble
9 P M -0.6091 Soluble
11 G M -0.5367 Soluble
12 L M -0.2946 Soluble
13 V M 0.0000 Buried
14 K M -2.0964 Soluble
15 P M -1.3754 Soluble
16 S M -1.1944 Soluble
17 D M -1.5480 Soluble
18 T M -1.1883 Soluble
19 L M 0.0000 Buried
20 S M -0.8431 Soluble
21 L M 0.0000 Buried
22 T M -0.8305 Soluble
23 C M 0.0000 Buried
24 A M -1.1960 Soluble
25 V M 0.0000 Buried
26 S M -1.2623 Soluble
27 G M -0.9942 Soluble
28 Y M -0.2038 Soluble
29 P M -0.2805 Soluble
30 I M 0.0000 Buried
31 R M -0.9822 Soluble
35 F M 1.2028 Aggregation-prone
36 G M 0.6278 Aggregation-prone
37 Y M 0.4827 Aggregation-prone
38 S M 0.0000 Buried
39 W M 0.0000 Buried
40 H M 0.0000 Buried
41 W M 0.0000 Buried
42 I M 0.0000 Buried
43 R M 0.0000 Buried
44 Q M -0.8386 Soluble
45 P M -0.6525 Soluble
46 P M -1.0288 Soluble
47 G M -1.5121 Soluble
48 K M -2.2937 Soluble
49 G M -1.4234 Soluble
50 L M 0.0000 Buried
51 E M -0.7780 Soluble
52 W M 0.0000 Buried
53 M M 0.0000 Buried
54 G M 0.0000 Buried
55 Y M 0.0000 Buried
56 I M 0.0000 Buried
57 H M 0.3347 Aggregation-prone
58 Y M 0.5433 Aggregation-prone
59 S M 0.2844 Aggregation-prone
63 G M 0.0255 Aggregation-prone
64 Y M 1.0164 Aggregation-prone
65 T M 0.2886 Aggregation-prone
66 D M -0.2739 Soluble
67 F M -0.2517 Soluble
68 N M 0.0000 Buried
69 P M -0.8430 Soluble
70 S M -0.6965 Soluble
71 L M 0.0000 Buried
72 K M -1.5937 Soluble
74 T M -0.9445 Soluble
75 R M -1.1713 Soluble
76 I M 0.0000 Buried
77 T M -0.8683 Soluble
78 I M 0.0000 Buried
79 S M -0.8579 Soluble
80 R M -1.6964 Soluble
81 D M -2.4237 Soluble
82 T M -1.9452 Soluble
83 S M -1.8375 Soluble
84 K M -2.4577 Soluble
85 N M -2.0198 Soluble
86 Q M -1.9002 Soluble
87 F M 0.0000 Buried
88 S M -0.9021 Soluble
89 L M 0.0000 Buried
90 K M -1.3877 Soluble
91 L M 0.0000 Buried
92 S M -1.0558 Soluble
93 S M -0.9029 Soluble
94 V M 0.0000 Buried
95 T M 0.0485 Aggregation-prone
96 A M 0.6236 Aggregation-prone
97 V M 1.7739 Aggregation-prone
98 D M 0.0000 Buried
99 T M 0.6328 Aggregation-prone
100 A M 0.0000 Buried
101 V M 0.0219 Aggregation-prone
102 Y M 0.0000 Buried
103 Y M 0.0000 Buried
104 C M 0.0000 Buried
105 A M 0.0000 Buried
106 R M 0.0000 Buried
107 K M -0.2736 Soluble
108 D M -0.2757 Soluble
109 S M -0.3758 Soluble
110 G M -0.3221 Soluble
113 N M -0.3058 Soluble
114 Y M -0.1127 Soluble
115 F M 0.0000 Buried
116 P M -0.1026 Soluble
117 Y M -0.3408 Soluble
118 W M -0.6501 Soluble
119 G M 0.0000 Buried
120 Q M -1.9747 Soluble
121 G M -1.1417 Soluble
122 T M -0.3734 Soluble
123 L M -0.0017 Soluble
124 V M 0.0000 Buried
125 T M 0.0406 Aggregation-prone
126 V M 0.0000 Buried
127 S M -0.6868 Soluble
128 S M -0.8910 Soluble
129 A M -0.6304 Soluble
130 S M -0.7909 Soluble
131 T M -0.9456 Soluble
132 K M -1.9035 Soluble
133 G M -1.8212 Soluble
134 P M 0.0000 Buried
135 S M -0.5783 Soluble
136 V M 0.0000 Buried
137 F M -0.6735 Soluble
138 P M -1.1642 Soluble
139 L M 0.0000 Buried
140 A M -0.5849 Soluble
141 P M 0.0000 Buried
142 S M -0.7190 Soluble
143 S M -1.1276 Soluble
144 K M -2.1389 Soluble
145 S M -1.4454 Soluble
146 T M -1.0802 Soluble
147 S M -1.1691 Soluble
148 G M -1.1630 Soluble
149 G M -0.9858 Soluble
150 T M -0.7352 Soluble
151 A M 0.0000 Buried
152 A M 0.0000 Buried
153 L M 0.0000 Buried
154 G M 0.0000 Buried
155 C M 0.0000 Buried
156 L M 0.0000 Buried
157 V M 0.0000 Buried
158 K M -0.3707 Soluble
159 D M -0.8260 Soluble
160 Y M 0.0000 Buried
161 F M 0.0000 Buried
162 P M 0.0000 Buried
163 E M -0.2436 Soluble
164 P M -0.5007 Soluble
165 V M -0.5351 Soluble
166 T M -0.5005 Soluble
167 V M -0.2111 Soluble
168 S M -0.2777 Soluble
169 W M 0.0000 Buried
170 N M -0.6664 Soluble
171 S M -0.5455 Soluble
172 G M -0.5986 Soluble
173 A M -0.2871 Soluble
174 L M -0.0658 Soluble
175 T M -0.2120 Soluble
176 S M -0.1935 Soluble
177 G M -0.1831 Soluble
178 V M 0.1292 Aggregation-prone
179 H M -0.1577 Soluble
180 T M 0.0141 Aggregation-prone
181 F M 0.0000 Buried
182 P M -0.3021 Soluble
183 A M 0.2475 Aggregation-prone
184 V M 0.4773 Aggregation-prone
185 L M 1.2510 Aggregation-prone
186 Q M 0.1969 Aggregation-prone
187 S M -0.0616 Soluble
188 S M -0.2406 Soluble
189 G M 0.0406 Aggregation-prone
190 L M -0.0706 Soluble
191 Y M 0.3915 Aggregation-prone
192 S M 0.0000 Buried
193 L M 0.0000 Buried
194 S M 0.0000 Buried
195 S M 0.0000 Buried
196 V M 0.0000 Buried
197 V M 0.0000 Buried
198 T M -0.1575 Soluble
199 V M 0.0000 Buried
200 P M -0.5131 Soluble
201 S M -0.8659 Soluble
202 S M -0.6793 Soluble
203 S M -0.5771 Soluble
204 L M -0.7947 Soluble
205 G M -0.9729 Soluble
206 T M -0.7300 Soluble
207 Q M -1.0730 Soluble
208 T M -1.1150 Soluble
209 Y M 0.0000 Buried
210 I M -1.2871 Soluble
211 C M 0.0000 Buried
212 N M -1.5620 Soluble
213 V M 0.0000 Buried
214 N M -1.6991 Soluble
215 H M 0.0000 Buried
216 K M -2.5332 Soluble
217 P M -1.6425 Soluble
218 S M -2.0046 Soluble
219 N M -2.5217 Soluble
220 T M -2.0062 Soluble
221 K M -2.5806 Soluble
222 V M -1.5603 Soluble
223 D M -2.7028 Soluble
224 K M -2.3218 Soluble
225 R M -2.7945 Soluble
226 V M 0.0000 Buried
227 E M -2.3951 Soluble
228 P M -1.1925 Soluble
1 E N -1.6220 Soluble
2 I N 0.0000 Buried
3 V N 0.6835 Aggregation-prone
4 L N 0.0000 Buried
5 T N -0.7997 Soluble
6 Q N 0.0000 Buried
7 S N -1.0078 Soluble
8 P N -0.7286 Soluble
9 D N -1.3588 Soluble
10 F N 0.3912 Aggregation-prone
11 Q N -0.1288 Soluble
12 S N -0.2684 Soluble
13 V N -1.3230 Soluble
14 T N -1.7981 Soluble
15 P N -2.3363 Soluble
16 K N -3.3993 Soluble
17 E N -3.8751 Soluble
18 K N -3.4743 Soluble
19 V N 0.0000 Buried
20 T N -0.8689 Soluble
21 I N 0.0000 Buried
22 T N -0.8080 Soluble
23 C N 0.0000 Buried
24 R N -2.1754 Soluble
25 A N 0.0000 Buried
26 S N -0.7234 Soluble
27 Q N -0.9850 Soluble
28 S N -1.1080 Soluble
29 I N 0.0000 Buried
36 S N -1.2162 Soluble
37 D N -1.8248 Soluble
38 H N -1.0048 Soluble
39 L N 0.0000 Buried
40 H N 0.0000 Buried
41 W N 0.0000 Buried
42 Y N 0.0000 Buried
43 Q N 0.0000 Buried
44 Q N 0.0000 Buried
45 K N -1.9474 Soluble
46 P N -1.8513 Soluble
47 D N -2.6719 Soluble
48 Q N -2.4181 Soluble
49 S N -1.4571 Soluble
50 P N 0.0000 Buried
51 K N -1.0948 Soluble
52 L N 0.0000 Buried
53 L N 0.0000 Buried
54 I N 0.0000 Buried
55 K N -0.6241 Soluble
56 Y N -0.6148 Soluble
57 A N 0.0000 Buried
65 S N -1.0109 Soluble
66 H N -0.9955 Soluble
67 A N -0.4580 Soluble
68 I N -0.1366 Soluble
69 S N -0.3291 Soluble
70 G N -0.4188 Soluble
71 V N -0.2628 Soluble
72 P N -0.3580 Soluble
74 S N -0.6272 Soluble
75 R N -1.1889 Soluble
76 F N 0.0000 Buried
77 S N -0.7575 Soluble
78 G N -0.6665 Soluble
79 S N -0.7320 Soluble
80 G N -1.2287 Soluble
83 S N -1.2263 Soluble
84 G N -1.3966 Soluble
85 T N -1.4308 Soluble
86 D N -1.8756 Soluble
87 F N 0.0000 Buried
88 T N -0.7344 Soluble
89 L N 0.0000 Buried
90 T N -0.8982 Soluble
91 I N 0.0000 Buried
92 N N -2.9502 Soluble
93 S N -2.9689 Soluble
94 L N 0.0000 Buried
95 E N -2.4712 Soluble
96 A N -2.5056 Soluble
97 E N -2.7806 Soluble
98 D N 0.0000 Buried
99 A N -1.4594 Soluble
100 A N 0.0000 Buried
101 T N -0.5403 Soluble
102 Y N 0.0000 Buried
103 Y N 0.0000 Buried
104 C N 0.0000 Buried
105 Q N 0.0000 Buried
106 Q N 0.0000 Buried
107 G N 0.0000 Buried
108 Y N -0.1435 Soluble
109 D N -1.1047 Soluble
114 F N -0.2401 Soluble
115 P N -0.4261 Soluble
116 L N 0.0000 Buried
117 T N -0.0764 Soluble
118 F N 0.1860 Aggregation-prone
119 G N 0.0000 Buried
120 G N -1.0341 Soluble
121 G N -0.9261 Soluble
122 T N 0.0000 Buried
123 K N -0.6845 Soluble
124 V N 0.0000 Buried
125 E N -0.5948 Soluble
126 I N 0.0000 Buried
127 K N -1.1979 Soluble
128 R N -0.8606 Soluble
129 T N 0.1195 Aggregation-prone
130 V N 0.9335 Aggregation-prone
131 A N 0.3712 Aggregation-prone
132 A N 0.0251 Aggregation-prone
133 P N 0.0000 Buried
134 S N -0.1428 Soluble
135 V N 0.0000 Buried
136 F N 0.1197 Aggregation-prone
137 I N 0.1564 Aggregation-prone
138 F N 0.0000 Buried
139 P N -0.6139 Soluble
140 P N 0.0000 Buried
141 S N -1.3065 Soluble
142 D N -2.5147 Soluble
143 E N -2.1389 Soluble
144 Q N 0.0000 Buried
145 L N -2.0459 Soluble
146 K N -2.5456 Soluble
147 S N -1.5960 Soluble
148 G N -1.3295 Soluble
149 T N -0.8730 Soluble
150 A N 0.0000 Buried
151 S N 0.0000 Buried
152 V N 0.0000 Buried
153 V N 0.0000 Buried
154 C N 0.0000 Buried
155 L N 0.0000 Buried
156 L N 0.0000 Buried
157 N N 0.0000 Buried
158 N N -0.7706 Soluble
159 F N 0.0000 Buried
160 Y N 0.0000 Buried
161 P N -1.5712 Soluble
162 R N -2.8353 Soluble
163 E N -3.1321 Soluble
164 A N -2.2974 Soluble
165 K N -2.2823 Soluble
166 V N -1.1809 Soluble
167 Q N -0.5690 Soluble
168 W N 0.0000 Buried
169 K N -0.5325 Soluble
170 V N 0.0000 Buried
171 D N -1.8414 Soluble
172 N N -1.4357 Soluble
173 A N -0.1981 Soluble
174 L N 0.8101 Aggregation-prone
175 Q N -0.0529 Soluble
176 S N -0.4837 Soluble
177 G N -0.9198 Soluble
178 N N -0.7893 Soluble
179 S N -1.2857 Soluble
180 Q N -1.8419 Soluble
181 E N -2.1315 Soluble
182 S N -0.8533 Soluble
183 V N -0.3801 Soluble
184 T N -0.5601 Soluble
185 E N -1.4636 Soluble
186 Q N 0.0000 Buried
187 D N -1.9590 Soluble
188 S N -2.4260 Soluble
189 K N -2.8957 Soluble
190 D N -2.2627 Soluble
191 S N 0.0000 Buried
192 T N 0.0000 Buried
193 Y N 0.0000 Buried
194 S N 0.0000 Buried
195 L N 0.0000 Buried
196 S N 0.0000 Buried
197 S N 0.0000 Buried
198 T N -0.8514 Soluble
199 L N 0.0000 Buried
200 T N -0.3158 Soluble
201 L N -0.6648 Soluble
202 S N -1.1679 Soluble
203 K N -2.3176 Soluble
204 A N -1.9873 Soluble
205 D N -2.9490 Soluble
206 Y N 0.0000 Buried
207 E N -4.0917 Soluble
208 K N -4.0352 Soluble
209 H N -3.5955 Soluble
210 K N -3.8718 Soluble
211 V N -1.9881 Soluble
212 Y N 0.0000 Buried
213 A N -0.6885 Soluble
214 C N 0.0000 Buried
215 E N -0.9746 Soluble
216 V N 0.0000 Buried
217 T N -1.1733 Soluble
218 H N 0.0000 Buried
219 Q N -1.5228 Soluble
220 G N -0.3561 Soluble
221 L N -0.2023 Soluble
222 S N -0.4693 Soluble
223 S N -0.4334 Soluble
224 P N -0.5962 Soluble
225 V N -0.1089 Soluble
226 T N -0.4712 Soluble
227 K N -0.5594 Soluble
228 S N -0.5808 Soluble
229 F N -1.0733 Soluble
230 N N -2.6012 Soluble
231 R N -3.5706 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
Load structure to view sequence…
Downloads
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7444 3.1313 CSV PDB
4.5 -0.7869 2.9471 CSV PDB
5.0 -0.8368 2.7377 CSV PDB
5.5 -0.8829 2.5986 CSV PDB
6.0 -0.9128 2.6061 CSV PDB
6.5 -0.9182 2.6105 CSV PDB
7.0 -0.901 2.6124 CSV PDB
7.5 -0.8694 2.613 CSV PDB
8.0 -0.8294 2.6131 CSV PDB
8.5 -0.7832 2.6127 CSV PDB
9.0 -0.7304 2.6114 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH