Ravagalimab / 6pe9

A4DAB219_6pe9_E_F | Source PDB: 6pe9 | Chains: E, F | Done
98.25
Yes
10 Å
Yes
Avg A4D Score
-0.7158
Max Score
1.3038
Min Score
-3.5847
Total Score
-307.8
Score CDR
-0.3788
Score non-CDR
-0.7592

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVKPGGSLRLSCAASGFTFSDYGMNWVRQAPGKGLEWVSYISSGRSNIYYADTVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCARSWGYFDVWGQGTTVTVSSASTKGPSVFPLAPSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPDIVMTQSPDSLAVSLGERATINCKSSQSLLNLGNQKNYLTWFQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQNDYTYPLTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) E (Heavy), F (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.5847
Maximum Score 1.3038
Average Score -0.7158
Total Score -307.8003

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E E -1.9842 Soluble
2 V E -0.9676 Soluble
3 Q E -0.9245 Soluble
4 L E 0.0000 Buried
5 V E 0.9712 Aggregation-prone
6 E E 0.0000 Buried
7 S E -0.4120 Soluble
8 G E -0.9839 Soluble
9 G E -0.5305 Soluble
11 G E -0.4579 Soluble
12 L E -0.0818 Soluble
13 V E -0.8552 Soluble
14 K E -2.1073 Soluble
15 P E -2.0179 Soluble
16 G E -1.6397 Soluble
17 G E -1.3186 Soluble
18 S E -1.2179 Soluble
19 L E -1.1662 Soluble
20 R E -2.0517 Soluble
21 L E 0.0000 Buried
22 S E -0.3763 Soluble
23 C E 0.0000 Buried
24 A E 0.0068 Aggregation-prone
25 A E 0.0000 Buried
26 S E -0.7620 Soluble
27 G E -1.1636 Soluble
28 F E -0.6667 Soluble
29 T E -0.6685 Soluble
30 F E 0.0000 Buried
35 S E -1.3014 Soluble
36 D E -1.5609 Soluble
37 Y E -0.4189 Soluble
38 G E 0.0000 Buried
39 M E 0.0000 Buried
40 N E 0.0000 Buried
41 W E 0.0000 Buried
42 V E 0.0000 Buried
43 R E 0.0000 Buried
44 Q E -0.8118 Soluble
45 A E -1.1992 Soluble
46 P E -0.9962 Soluble
47 G E -1.4672 Soluble
48 K E -2.3127 Soluble
49 G E -1.4716 Soluble
50 L E 0.0000 Buried
51 E E -1.1860 Soluble
52 W E 0.0000 Buried
53 V E 0.0000 Buried
54 S E 0.0000 Buried
55 Y E 0.7355 Aggregation-prone
56 I E 0.0000 Buried
57 S E -0.5619 Soluble
58 S E -1.3161 Soluble
59 G E -1.8253 Soluble
62 R E -2.1656 Soluble
63 S E -1.2019 Soluble
64 N E -0.5107 Soluble
65 I E 0.9964 Aggregation-prone
66 Y E 0.9286 Aggregation-prone
67 Y E -0.1343 Soluble
68 A E 0.0000 Buried
69 D E -2.5554 Soluble
70 T E -1.7140 Soluble
71 V E 0.0000 Buried
72 K E -2.4968 Soluble
74 G E -1.8009 Soluble
75 R E -1.5483 Soluble
76 F E 0.0000 Buried
77 T E -0.5771 Soluble
78 I E 0.0000 Buried
79 S E -0.2798 Soluble
80 R E -0.9995 Soluble
81 D E -1.5492 Soluble
82 N E -1.9796 Soluble
83 A E -1.4405 Soluble
84 K E -2.3092 Soluble
85 N E -1.7444 Soluble
86 S E -1.0230 Soluble
87 L E 0.0000 Buried
88 Y E -0.4614 Soluble
89 L E 0.0000 Buried
90 Q E -1.1858 Soluble
91 M E 0.0000 Buried
92 N E -1.4964 Soluble
93 S E -1.4096 Soluble
94 L E 0.0000 Buried
95 R E -2.6100 Soluble
96 A E -1.8950 Soluble
97 E E -2.3125 Soluble
98 D E 0.0000 Buried
99 T E -0.7516 Soluble
100 A E 0.0000 Buried
101 V E 0.1480 Aggregation-prone
102 Y E 0.0000 Buried
103 Y E 0.0000 Buried
104 C E 0.0000 Buried
105 A E 0.0000 Buried
106 R E 0.0000 Buried
107 S E 0.0000 Buried
108 W E 0.7944 Aggregation-prone
109 G E 0.2850 Aggregation-prone
114 Y E 0.3855 Aggregation-prone
115 F E 0.0000 Buried
116 D E -0.3294 Soluble
117 V E -0.0520 Soluble
118 W E -0.3512 Soluble
119 G E 0.0000 Buried
120 Q E -1.1633 Soluble
121 G E -0.4984 Soluble
122 T E 0.0000 Buried
123 T E -0.0775 Soluble
124 V E 0.0000 Buried
125 T E 0.0000 Buried
126 V E 0.0000 Buried
127 S E -0.9741 Soluble
128 S E -0.7966 Soluble
129 A E -0.6071 Soluble
130 S E -0.7773 Soluble
131 T E -0.8980 Soluble
132 K E -1.7322 Soluble
133 G E -1.7110 Soluble
134 P E -0.7888 Soluble
135 S E -0.3256 Soluble
136 V E 0.0000 Buried
137 F E -0.6157 Soluble
138 P E -1.0663 Soluble
139 L E 0.0000 Buried
140 A E -0.7367 Soluble
141 P E 0.0000 Buried
142 S E -0.2859 Soluble
143 G E -0.6082 Soluble
144 T E -0.4779 Soluble
145 A E -0.2731 Soluble
146 A E 0.0000 Buried
147 L E 0.0000 Buried
148 G E 0.0000 Buried
149 C E 0.0000 Buried
150 L E 0.0000 Buried
151 V E 0.0000 Buried
152 K E 0.0000 Buried
153 D E -0.6439 Soluble
154 Y E 0.0000 Buried
155 F E 0.0000 Buried
156 P E 0.0000 Buried
157 E E -0.4827 Soluble
158 P E -0.7504 Soluble
159 V E -0.7324 Soluble
160 T E -0.6089 Soluble
161 V E 0.0015 Aggregation-prone
162 S E -0.1443 Soluble
163 W E 0.0000 Buried
164 N E -0.5914 Soluble
165 S E -0.5369 Soluble
166 G E -0.3864 Soluble
167 A E -0.1089 Soluble
168 L E 0.0687 Aggregation-prone
169 T E -0.1069 Soluble
170 S E -0.1914 Soluble
171 G E -0.2221 Soluble
172 V E 0.2043 Aggregation-prone
173 H E 0.0000 Buried
174 T E 0.0474 Aggregation-prone
175 F E 0.0000 Buried
176 P E -0.1670 Soluble
177 A E 0.4019 Aggregation-prone
178 V E 0.7233 Aggregation-prone
179 L E 1.3038 Aggregation-prone
180 Q E 0.2535 Aggregation-prone
181 S E -0.0532 Soluble
182 S E -0.2935 Soluble
183 G E -0.1514 Soluble
184 L E -0.0584 Soluble
185 Y E 0.3534 Aggregation-prone
186 S E 0.2673 Aggregation-prone
187 L E 0.0000 Buried
188 S E 0.0000 Buried
189 S E 0.0000 Buried
190 V E 0.0000 Buried
191 V E 0.0000 Buried
192 T E -0.1107 Soluble
193 V E 0.0000 Buried
194 P E -0.4680 Soluble
195 S E -0.5086 Soluble
196 S E -0.5766 Soluble
197 S E -0.6007 Soluble
198 L E -0.8120 Soluble
199 G E -1.1072 Soluble
200 T E -0.7522 Soluble
201 Q E -1.0236 Soluble
202 T E -1.0399 Soluble
203 Y E 0.0000 Buried
204 I E -1.0539 Soluble
205 C E 0.0000 Buried
206 N E -1.4008 Soluble
207 V E 0.0000 Buried
208 N E -2.0380 Soluble
209 H E 0.0000 Buried
210 K E -2.7333 Soluble
211 P E -1.6349 Soluble
212 S E -1.9629 Soluble
213 N E -2.4720 Soluble
214 T E -2.1723 Soluble
215 K E -2.7413 Soluble
216 V E -1.5463 Soluble
217 D E -2.6030 Soluble
218 K E -2.0920 Soluble
219 K E -2.5437 Soluble
220 V E 0.0000 Buried
221 E E -2.3956 Soluble
222 P E -1.1231 Soluble
1 D F -1.6333 Soluble
2 I F 0.0000 Buried
3 V F 0.7393 Aggregation-prone
4 M F 0.0000 Buried
5 T F -0.6966 Soluble
6 Q F 0.0000 Buried
7 S F -1.0953 Soluble
8 P F -1.2579 Soluble
9 D F -2.2844 Soluble
10 S F -1.4698 Soluble
11 L F -0.7894 Soluble
12 A F -0.7872 Soluble
13 V F -1.2567 Soluble
14 S F -1.5776 Soluble
15 L F -0.8801 Soluble
16 G F -1.6522 Soluble
17 E F -2.8623 Soluble
18 R F -2.8047 Soluble
19 A F 0.0000 Buried
20 T F -0.7650 Soluble
21 I F 0.0000 Buried
22 N F -1.3727 Soluble
23 C F 0.0000 Buried
24 K F -1.9591 Soluble
25 S F -0.9516 Soluble
26 S F -0.8627 Soluble
27 Q F -1.5136 Soluble
28 S F -1.0596 Soluble
29 L F 0.0000 Buried
30 L F -0.2165 Soluble
31 N F -0.3948 Soluble
32 L F 0.3917 Aggregation-prone
33 G F -0.7113 Soluble
34 N F -1.5748 Soluble
35 Q F -1.5358 Soluble
36 K F -1.0579 Soluble
37 N F -0.3855 Soluble
38 Y F 0.0837 Aggregation-prone
39 L F 0.0000 Buried
40 T F 0.0000 Buried
41 W F 0.0000 Buried
42 F F 0.0000 Buried
43 Q F 0.0000 Buried
44 Q F -1.1162 Soluble
45 K F -1.6133 Soluble
46 P F -1.2697 Soluble
47 G F -1.2261 Soluble
48 Q F -1.6444 Soluble
49 P F -1.2375 Soluble
50 P F 0.0000 Buried
51 K F -1.6919 Soluble
52 L F 0.0000 Buried
53 L F 0.0000 Buried
54 I F 0.0000 Buried
55 Y F -0.1597 Soluble
56 W F 0.0596 Aggregation-prone
57 A F 0.0000 Buried
65 S F -0.5759 Soluble
66 T F -0.6341 Soluble
67 R F -1.7074 Soluble
68 E F -1.3081 Soluble
69 S F -0.8755 Soluble
70 G F -1.0967 Soluble
71 V F 0.0000 Buried
72 P F -1.4546 Soluble
74 D F -2.2322 Soluble
75 R F -1.7042 Soluble
76 F F 0.0000 Buried
77 S F -0.8879 Soluble
78 G F -0.5209 Soluble
79 S F -0.7479 Soluble
80 G F -1.2705 Soluble
83 S F -1.1669 Soluble
84 G F -1.2300 Soluble
85 T F -1.7312 Soluble
86 D F -2.6831 Soluble
87 F F 0.0000 Buried
88 T F -0.9906 Soluble
89 L F 0.0000 Buried
90 T F -0.6849 Soluble
91 I F 0.0000 Buried
92 S F -1.9917 Soluble
93 S F -1.7388 Soluble
94 L F 0.0000 Buried
95 Q F -1.6207 Soluble
96 A F -1.7708 Soluble
97 E F -1.8502 Soluble
98 D F 0.0000 Buried
99 V F 0.0000 Buried
100 A F 0.0000 Buried
101 V F -0.8490 Soluble
102 Y F 0.0000 Buried
103 Y F 0.0000 Buried
104 C F 0.0000 Buried
105 Q F 0.0000 Buried
106 N F 0.0000 Buried
107 D F 0.2646 Aggregation-prone
108 Y F 0.4241 Aggregation-prone
109 T F 0.2576 Aggregation-prone
114 Y F 0.5379 Aggregation-prone
115 P F -0.2523 Soluble
116 L F 0.0000 Buried
117 T F -0.0209 Soluble
118 F F 0.0296 Aggregation-prone
119 G F 0.0000 Buried
120 Q F -1.2639 Soluble
121 G F 0.0000 Buried
122 T F 0.0000 Buried
123 K F -1.8336 Soluble
124 L F 0.0000 Buried
125 E F -1.7210 Soluble
126 I F 0.0000 Buried
127 K F -1.8027 Soluble
128 R F -1.2113 Soluble
129 T F -0.2589 Soluble
130 V F 0.4058 Aggregation-prone
131 A F 0.0823 Aggregation-prone
132 A F -0.1537 Soluble
133 P F -0.2934 Soluble
134 S F -0.2795 Soluble
135 V F 0.0000 Buried
136 F F 0.0923 Aggregation-prone
137 I F 0.0943 Aggregation-prone
138 F F 0.0000 Buried
139 P F -0.8200 Soluble
140 P F 0.0000 Buried
141 S F -1.4385 Soluble
142 D F -2.6960 Soluble
143 E F -2.5725 Soluble
144 Q F 0.0000 Buried
145 L F -2.0610 Soluble
146 K F -2.6391 Soluble
147 S F -1.5917 Soluble
148 G F -1.2856 Soluble
149 T F -0.9320 Soluble
150 A F 0.0000 Buried
151 S F 0.0000 Buried
152 V F 0.0000 Buried
153 V F 0.0000 Buried
154 C F 0.0000 Buried
155 L F 0.0000 Buried
156 L F 0.0000 Buried
157 N F 0.0000 Buried
158 N F -0.8970 Soluble
159 F F 0.0000 Buried
160 Y F 0.0000 Buried
161 P F -1.8859 Soluble
162 R F -3.2542 Soluble
163 E F -3.2668 Soluble
164 A F -2.4991 Soluble
165 K F -2.6489 Soluble
166 V F -1.3530 Soluble
167 Q F -0.9987 Soluble
168 W F 0.0000 Buried
169 K F -0.7764 Soluble
170 V F 0.0000 Buried
171 D F -1.5290 Soluble
172 N F -1.2248 Soluble
173 A F -0.1649 Soluble
174 L F 0.6557 Aggregation-prone
175 Q F -0.2664 Soluble
176 S F -0.5308 Soluble
177 G F -0.9661 Soluble
178 N F -0.8770 Soluble
179 S F -1.1173 Soluble
180 Q F -1.2919 Soluble
181 E F -1.2980 Soluble
182 S F 0.0000 Buried
183 V F -0.6360 Soluble
184 T F -1.1966 Soluble
185 E F -2.3421 Soluble
186 Q F 0.0000 Buried
187 D F -1.9765 Soluble
188 S F -2.1831 Soluble
189 K F -2.4415 Soluble
190 D F -1.6556 Soluble
191 S F 0.0000 Buried
192 T F 0.0000 Buried
193 Y F 0.0000 Buried
194 S F 0.0000 Buried
195 L F 0.0000 Buried
196 S F 0.0000 Buried
197 S F 0.0000 Buried
198 T F -0.5694 Soluble
199 L F 0.0000 Buried
200 T F -0.2731 Soluble
201 L F -0.3784 Soluble
202 S F -1.0633 Soluble
203 K F -2.0899 Soluble
204 A F -1.9804 Soluble
205 D F -3.0020 Soluble
206 Y F 0.0000 Buried
207 E F -3.3164 Soluble
208 K F -3.5847 Soluble
209 H F -3.0272 Soluble
210 K F -3.0985 Soluble
211 V F -1.4537 Soluble
212 Y F 0.0000 Buried
213 A F 0.0000 Buried
214 C F 0.0000 Buried
215 E F -1.1184 Soluble
216 V F 0.0000 Buried
217 T F -1.2392 Soluble
218 H F 0.0000 Buried
219 Q F -1.6942 Soluble
220 G F -0.3772 Soluble
221 L F -0.1759 Soluble
222 S F -0.4342 Soluble
223 S F -0.3830 Soluble
224 P F -0.4920 Soluble
225 V F 0.0544 Aggregation-prone
226 T F -0.5681 Soluble
227 K F -0.7204 Soluble
228 S F -0.6987 Soluble
229 F F -0.9936 Soluble
230 N F -1.9977 Soluble
231 R F -2.5075 Soluble
232 G F -1.9117 Soluble
233 E F -2.2731 Soluble
234 C F -0.5069 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6762 3.5188 CSV PDB
4.5 -0.7222 3.4622 CSV PDB
5.0 -0.7755 3.3967 CSV PDB
5.5 -0.8258 3.3275 CSV PDB
6.0 -0.8613 3.2572 CSV PDB
6.5 -0.8735 3.1865 CSV PDB
7.0 -0.8625 3.1161 CSV PDB
7.5 -0.8359 3.0471 CSV PDB
8.0 -0.8007 2.9817 CSV PDB
8.5 -0.7589 2.9248 CSV PDB
9.0 -0.7098 2.8821 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH