Eblasakimab / 4hwe

A4DAB095_4hwe_H_L | Source PDB: 4hwe | Chains: H, L | Done
97.75
Yes
10 Å
Yes
Avg A4D Score
-0.6891
Max Score
1.1457
Min Score
-3.3799
Total Score
-291.5
Score CDR
-0.2604
Score non-CDR
-0.7364

Calculation Parameters

Chain Sequence(s) EVQLVQSGAEVKKPGESLKISCKGSGYSFTSYWIGWVRQMPGKGLEWMGVIYPGDSYTRYSPSFQGQVTISADKSISTAYLQWSSLKASDTAMYYCARMPNWGSFDYWGQGTLVTVSSASTKGPSVFPLAPGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKEVVLTQSPGTLSLSPGERATLSCRASQSISSSYLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYETFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.3799
Maximum Score 1.1457
Average Score -0.6891
Total Score -291.5027

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.1632 Soluble
2 V H -1.3165 Soluble
3 Q H -1.5353 Soluble
4 L H 0.0000 Buried
5 V H 0.1945 Aggregation-prone
6 Q H 0.0000 Buried
7 S H -0.4450 Soluble
8 G H -0.1318 Soluble
9 A H 0.2967 Aggregation-prone
11 E H 0.2747 Aggregation-prone
12 V H 1.1190 Aggregation-prone
13 K H -0.6749 Soluble
14 K H -1.6540 Soluble
15 P H -1.9934 Soluble
16 G H -1.8890 Soluble
17 E H -2.0239 Soluble
18 S H -1.7163 Soluble
19 L H 0.0000 Buried
20 K H -1.9684 Soluble
21 I H 0.0000 Buried
22 S H -0.5127 Soluble
23 C H 0.0000 Buried
24 K H -0.6270 Soluble
25 G H 0.0000 Buried
26 S H -0.8825 Soluble
27 G H -1.3401 Soluble
28 Y H -0.5806 Soluble
29 S H -0.2142 Soluble
30 F H 0.0000 Buried
35 T H -0.7402 Soluble
36 S H -0.3444 Soluble
37 Y H 0.0526 Aggregation-prone
38 W H 0.2469 Aggregation-prone
39 I H 0.0000 Buried
40 G H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H -0.8733 Soluble
44 Q H -1.0198 Soluble
45 M H -0.9225 Soluble
46 P H -0.9895 Soluble
47 G H -1.4463 Soluble
48 K H -2.4131 Soluble
49 G H -1.6942 Soluble
50 L H 0.0000 Buried
51 E H -1.7186 Soluble
52 W H 0.0000 Buried
53 M H 0.0000 Buried
54 G H 0.0000 Buried
55 V H 0.0000 Buried
56 I H 0.0000 Buried
57 Y H -0.2311 Soluble
58 P H 0.0000 Buried
59 G H -1.1903 Soluble
62 D H -1.7170 Soluble
63 S H -0.8238 Soluble
64 Y H 0.5406 Aggregation-prone
65 T H 0.0004 Aggregation-prone
66 R H -0.6737 Soluble
67 Y H -0.8257 Soluble
68 S H 0.0000 Buried
69 P H -1.0990 Soluble
70 S H -0.9040 Soluble
71 F H 0.0000 Buried
72 Q H -1.5333 Soluble
74 G H -1.4300 Soluble
75 Q H -1.8011 Soluble
76 V H 0.0000 Buried
77 T H -0.8256 Soluble
78 I H 0.0000 Buried
79 S H -0.2845 Soluble
80 A H -0.8449 Soluble
81 D H -1.3396 Soluble
82 K H -2.0139 Soluble
83 S H -0.4954 Soluble
84 I H 0.5795 Aggregation-prone
85 S H -0.5237 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.6239 Soluble
89 L H 0.0000 Buried
90 Q H -1.3719 Soluble
91 W H 0.0000 Buried
92 S H -1.1738 Soluble
93 S H -1.6302 Soluble
94 L H 0.0000 Buried
95 K H -2.3890 Soluble
96 A H -1.2374 Soluble
97 S H -0.7508 Soluble
98 D H -0.8203 Soluble
99 T H -0.1730 Soluble
100 A H 0.0000 Buried
101 M H 0.2699 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 M H 0.0000 Buried
108 P H 0.0000 Buried
109 N H -0.8516 Soluble
110 W H 0.2229 Aggregation-prone
113 G H 0.0000 Buried
114 S H 0.0000 Buried
115 F H 0.0000 Buried
116 D H -0.2030 Soluble
117 Y H -0.1080 Soluble
118 W H -0.2389 Soluble
119 G H 0.0000 Buried
120 Q H -1.1008 Soluble
121 G H -0.4396 Soluble
122 T H 0.0000 Buried
123 L H 0.8700 Aggregation-prone
124 V H 0.0000 Buried
125 T H 0.1815 Aggregation-prone
126 V H 0.0000 Buried
127 S H -0.6741 Soluble
128 S H -0.9397 Soluble
129 A H -0.4052 Soluble
130 S H -0.4761 Soluble
131 T H -0.5353 Soluble
132 K H -1.1679 Soluble
133 G H -1.3269 Soluble
134 P H -0.5564 Soluble
135 S H -0.4050 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.1285 Soluble
139 L H 0.0000 Buried
140 A H -1.2054 Soluble
141 P H -0.8199 Soluble
142 G H -0.7819 Soluble
143 G H -0.8786 Soluble
144 T H -0.6166 Soluble
145 A H -0.3194 Soluble
146 A H 0.0000 Buried
147 L H 0.0000 Buried
148 G H 0.0000 Buried
149 C H 0.0000 Buried
150 L H 0.0000 Buried
151 V H 0.0000 Buried
152 K H 0.0000 Buried
153 D H -0.4312 Soluble
154 Y H 0.0000 Buried
155 F H 0.0000 Buried
156 P H -0.6311 Soluble
157 E H -0.8564 Soluble
158 P H -1.0125 Soluble
159 V H -0.7521 Soluble
160 T H -0.5406 Soluble
161 V H -0.3112 Soluble
162 S H -0.3908 Soluble
163 W H 0.0000 Buried
164 N H -0.8227 Soluble
165 S H -0.7104 Soluble
166 G H -0.6019 Soluble
167 A H -0.2832 Soluble
168 L H -0.0396 Soluble
169 T H -0.1775 Soluble
170 S H -0.1475 Soluble
171 G H -0.1356 Soluble
172 V H 0.2096 Aggregation-prone
173 H H -0.2876 Soluble
174 T H 0.0347 Aggregation-prone
175 F H 0.0000 Buried
176 P H -0.4419 Soluble
177 A H -0.0202 Soluble
178 V H 0.2788 Aggregation-prone
179 L H 0.8542 Aggregation-prone
180 Q H 0.1530 Aggregation-prone
181 S H -0.1130 Soluble
182 S H -0.2553 Soluble
183 G H -0.0511 Soluble
184 L H -0.0401 Soluble
185 Y H 0.0000 Buried
186 S H 0.0000 Buried
187 L H 0.0000 Buried
188 S H 0.0000 Buried
189 S H 0.0000 Buried
190 V H 0.0000 Buried
191 V H 0.0000 Buried
192 T H -0.1134 Soluble
193 V H 0.0000 Buried
194 P H -0.5416 Soluble
195 S H -0.6311 Soluble
196 S H -0.6016 Soluble
197 S H 0.0000 Buried
198 L H -0.6828 Soluble
199 G H -0.9285 Soluble
200 T H -0.7504 Soluble
201 Q H -1.1261 Soluble
202 T H -1.1820 Soluble
203 Y H 0.0000 Buried
204 I H -1.3309 Soluble
205 C H 0.0000 Buried
206 N H -1.4598 Soluble
207 V H 0.0000 Buried
208 N H -1.7398 Soluble
209 H H 0.0000 Buried
210 K H -2.7660 Soluble
211 P H -1.7297 Soluble
212 S H -1.8305 Soluble
213 N H -2.5195 Soluble
214 T H -2.0202 Soluble
215 K H -2.5805 Soluble
216 V H -1.6851 Soluble
217 D H -2.5037 Soluble
218 K H -1.9898 Soluble
219 K H -2.5604 Soluble
220 V H 0.0000 Buried
221 E H -2.7388 Soluble
222 P H -1.8497 Soluble
223 K H -2.2382 Soluble
1 E L -1.4789 Soluble
2 V L -0.1445 Soluble
3 V L 0.7843 Aggregation-prone
4 L L 0.0000 Buried
5 T L -0.5980 Soluble
6 Q L 0.0000 Buried
7 S L -0.7399 Soluble
8 P L -0.4642 Soluble
9 G L -0.8443 Soluble
10 T L -0.4124 Soluble
11 L L -0.2226 Soluble
12 S L -0.5201 Soluble
13 L L -0.9700 Soluble
14 S L -1.5500 Soluble
15 P L -1.9458 Soluble
16 G L -2.2573 Soluble
17 E L -2.8591 Soluble
18 R L -3.2284 Soluble
19 A L 0.0000 Buried
20 T L -0.5366 Soluble
21 L L 0.0000 Buried
22 S L -0.9306 Soluble
23 C L 0.0000 Buried
24 R L -2.1314 Soluble
25 A L 0.0000 Buried
26 S L -0.9299 Soluble
27 Q L -1.6918 Soluble
28 S L -1.3697 Soluble
29 I L 0.0000 Buried
30 S L -0.2850 Soluble
36 S L -0.5643 Soluble
37 S L -0.0600 Soluble
38 Y L 0.5893 Aggregation-prone
39 L L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.5216 Soluble
46 P L -1.4250 Soluble
47 G L -1.3071 Soluble
48 Q L -2.0418 Soluble
49 A L -1.3218 Soluble
50 P L 0.0000 Buried
51 R L -1.2596 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.0724 Aggregation-prone
56 G L -0.1012 Soluble
57 A L 0.0000 Buried
65 S L -0.6205 Soluble
66 S L -0.5971 Soluble
67 R L -1.2549 Soluble
68 A L -0.6952 Soluble
69 T L -0.5369 Soluble
70 G L -0.8726 Soluble
71 I L 0.0000 Buried
72 P L -1.3067 Soluble
74 D L -2.2101 Soluble
75 R L -2.0298 Soluble
76 F L 0.0000 Buried
77 S L -0.9669 Soluble
78 G L -0.5187 Soluble
79 S L -0.8532 Soluble
80 G L -1.3417 Soluble
83 S L -1.2811 Soluble
84 G L -1.3824 Soluble
85 T L -2.0136 Soluble
86 D L -2.7506 Soluble
87 F L 0.0000 Buried
88 T L -0.9127 Soluble
89 L L 0.0000 Buried
90 T L -0.8455 Soluble
91 I L 0.0000 Buried
92 S L -2.4228 Soluble
93 R L -3.3799 Soluble
94 L L 0.0000 Buried
95 E L -2.5433 Soluble
96 P L -1.7544 Soluble
97 E L -2.2604 Soluble
98 D L 0.0000 Buried
99 F L -0.6509 Soluble
100 A L 0.0000 Buried
101 V L -0.2593 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 Y L 0.6836 Aggregation-prone
116 E L -0.1967 Soluble
117 T L -0.0377 Soluble
118 F L -0.0294 Soluble
119 G L 0.0000 Buried
120 Q L -1.4258 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -0.5751 Soluble
124 V L 0.0000 Buried
125 E L 0.0000 Buried
126 I L -0.7413 Soluble
127 K L -1.3749 Soluble
128 R L -0.8788 Soluble
129 T L 0.1833 Aggregation-prone
130 V L 1.1457 Aggregation-prone
131 A L 0.3526 Aggregation-prone
132 A L 0.0581 Aggregation-prone
133 P L 0.0000 Buried
134 S L -0.2092 Soluble
135 V L 0.0000 Buried
136 F L 0.1076 Aggregation-prone
137 I L 0.1028 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.6966 Soluble
140 P L 0.0000 Buried
141 S L -1.7870 Soluble
142 D L -2.9121 Soluble
143 E L -2.9048 Soluble
144 Q L 0.0000 Buried
145 L L -2.1798 Soluble
146 K L -2.7494 Soluble
147 S L -1.6819 Soluble
148 G L -1.1640 Soluble
149 T L -0.9180 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.8334 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.3214 Soluble
162 R L -2.5576 Soluble
163 E L -2.9331 Soluble
164 A L -2.1669 Soluble
165 K L -2.1939 Soluble
166 V L -0.9760 Soluble
167 Q L -0.3884 Soluble
168 W L 0.0000 Buried
169 K L -0.2468 Soluble
170 V L 0.0000 Buried
171 D L -1.6499 Soluble
172 N L -1.4067 Soluble
173 A L -0.1262 Soluble
174 L L 0.8344 Aggregation-prone
175 Q L -0.1778 Soluble
176 S L -0.5784 Soluble
177 G L -1.1865 Soluble
178 N L -1.4050 Soluble
179 S L -1.4302 Soluble
180 Q L -1.5383 Soluble
181 E L -1.9667 Soluble
182 S L -0.8800 Soluble
183 V L -0.8335 Soluble
184 T L -1.1365 Soluble
185 E L -2.2011 Soluble
186 Q L -1.7638 Soluble
187 D L -1.9375 Soluble
188 S L -1.7364 Soluble
189 K L -2.3089 Soluble
190 D L -1.8502 Soluble
191 S L -1.7294 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.7244 Soluble
199 L L 0.0000 Buried
200 T L -0.5790 Soluble
201 L L -0.7007 Soluble
202 S L -0.9166 Soluble
203 K L -1.8897 Soluble
204 A L -1.6753 Soluble
205 D L -2.1513 Soluble
206 Y L 0.0000 Buried
207 E L -3.1883 Soluble
208 K L -3.3339 Soluble
209 H L -2.8591 Soluble
210 K L -3.1462 Soluble
211 V L -1.2902 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.7879 Soluble
216 V L 0.0000 Buried
217 T L -1.1899 Soluble
218 H L 0.0000 Buried
219 Q L -1.3929 Soluble
220 G L -0.1997 Soluble
221 L L -0.1152 Soluble
222 S L -0.4358 Soluble
223 S L -0.4333 Soluble
224 P L -0.6050 Soluble
225 V L -0.1300 Soluble
226 T L -0.5517 Soluble
227 K L -0.7544 Soluble
228 S L -0.6466 Soluble
229 F L -0.9856 Soluble
230 N L -1.9970 Soluble
231 R L -2.5936 Soluble
232 G L -2.1962 Soluble
233 E L -2.5397 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.701 1.6284 CSV PDB
4.5 -0.7459 1.5653 CSV PDB
5.0 -0.7988 1.5594 CSV PDB
5.5 -0.8495 1.5594 CSV PDB
6.0 -0.8861 1.5594 CSV PDB
6.5 -0.8982 1.5594 CSV PDB
7.0 -0.8842 1.5594 CSV PDB
7.5 -0.8517 1.5594 CSV PDB
8.0 -0.8083 1.5593 CSV PDB
8.5 -0.7573 1.5591 CSV PDB
9.0 -0.7 1.5602 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH