Opicinumab / 4oqt

A4DAB195_4oqt_H_L | Source PDB: 4oqt | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7376
Max Score
1.3562
Min Score
-3.6846
Total Score
-320.87
Score CDR
-0.4449
Score non-CDR
-0.7722

Calculation Parameters

Chain Sequence(s) EVQLLESGGGLVQPGGSLRLSCAASGFTFSAYEMKWVRQAPGKGLEWVSVIGPSGGFTFYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCATEGDNDAFDIWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDIQMTQSPATLSLSPGERATLSCRASQSVSSYLAWYQQKPGQAPRLLIYDASNRATGIPARFSGSGSGTDFTLTISSLEPEDFAVYYCQQRSNWPMYTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.6846
Maximum Score 1.3562
Average Score -0.7376
Total Score -320.8662

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -1.9984 Soluble
2 V H -1.1153 Soluble
3 Q H -1.4093 Soluble
4 L H 0.0000 Buried
5 L H 0.0120 Aggregation-prone
6 E H -0.3762 Soluble
7 S H -0.6708 Soluble
8 G H -0.8896 Soluble
9 G H -0.7324 Soluble
11 G H -0.3969 Soluble
12 L H -0.1929 Soluble
13 V H 0.0000 Buried
14 Q H -1.6471 Soluble
15 P H -1.7469 Soluble
16 G H -1.5162 Soluble
17 G H -1.2271 Soluble
18 S H -1.3701 Soluble
19 L H -1.1695 Soluble
20 R H -2.0333 Soluble
21 L H 0.0000 Buried
22 S H -0.4853 Soluble
23 C H 0.0000 Buried
24 A H -0.3300 Soluble
25 A H -0.6309 Soluble
26 S H -0.7498 Soluble
27 G H -1.0819 Soluble
28 F H -0.5273 Soluble
29 T H -0.0723 Soluble
30 F H 0.0000 Buried
35 S H -0.4330 Soluble
36 A H -0.0751 Soluble
37 Y H -0.4495 Soluble
38 E H -0.2732 Soluble
39 M H 0.0000 Buried
40 K H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -1.0045 Soluble
45 A H -1.2788 Soluble
46 P H -1.0265 Soluble
47 G H -1.6691 Soluble
48 K H -2.5428 Soluble
49 G H -1.9227 Soluble
50 L H 0.0000 Buried
51 E H -1.2095 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 V H 0.0000 Buried
56 I H 0.0000 Buried
57 G H 0.0000 Buried
58 P H -0.3524 Soluble
59 S H -0.5367 Soluble
62 G H -0.6116 Soluble
63 G H -0.3577 Soluble
64 F H 0.3984 Aggregation-prone
65 T H 0.3404 Aggregation-prone
66 F H 0.1703 Aggregation-prone
67 Y H -0.7926 Soluble
68 A H 0.0000 Buried
69 D H -2.3951 Soluble
70 S H -1.6459 Soluble
71 V H 0.0000 Buried
72 K H -2.5496 Soluble
74 G H -1.8789 Soluble
75 R H -1.5573 Soluble
76 F H 0.0000 Buried
77 T H -0.8283 Soluble
78 I H 0.0000 Buried
79 S H -0.2853 Soluble
80 R H -0.9613 Soluble
81 D H -1.4479 Soluble
82 N H -1.6465 Soluble
83 S H -1.5350 Soluble
84 K H -2.2978 Soluble
85 N H -1.6105 Soluble
86 T H -0.9428 Soluble
87 L H 0.0000 Buried
88 Y H -0.3200 Soluble
89 L H 0.0000 Buried
90 Q H -1.1890 Soluble
91 M H 0.0000 Buried
92 N H -1.7670 Soluble
93 S H -1.4563 Soluble
94 L H 0.0000 Buried
95 R H -2.4269 Soluble
96 A H -1.7679 Soluble
97 E H -2.2589 Soluble
98 D H 0.0000 Buried
99 T H -0.8513 Soluble
100 A H 0.0000 Buried
101 V H 0.0478 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 T H 0.0000 Buried
107 E H -0.8932 Soluble
108 G H 0.0000 Buried
109 D H -2.4759 Soluble
110 N H -2.4414 Soluble
113 D H -1.6518 Soluble
114 A H -1.0049 Soluble
115 F H -0.4954 Soluble
116 D H 0.0000 Buried
117 I H -0.1431 Soluble
118 W H 0.0000 Buried
119 G H 0.0000 Buried
120 Q H -1.5526 Soluble
121 G H -0.8634 Soluble
122 T H -0.3431 Soluble
123 T H -0.1411 Soluble
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -1.0011 Soluble
128 S H -0.7536 Soluble
129 A H -0.4829 Soluble
130 S H -0.5739 Soluble
131 T H -0.5503 Soluble
132 K H -1.0621 Soluble
133 G H -1.2434 Soluble
134 P H 0.0000 Buried
135 S H -0.2227 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.2138 Soluble
139 L H 0.0000 Buried
140 A H -1.2401 Soluble
141 P H 0.0000 Buried
142 S H -1.2508 Soluble
143 S H -1.5315 Soluble
144 K H -1.9184 Soluble
145 S H -1.1738 Soluble
146 T H -0.9176 Soluble
147 S H -0.8158 Soluble
148 G H -0.7888 Soluble
149 G H -0.5981 Soluble
150 T H -0.4284 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H 0.0000 Buried
159 D H -0.3046 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H 0.0000 Buried
163 E H -0.5135 Soluble
164 P H -0.7815 Soluble
165 V H -0.6762 Soluble
166 T H -0.6214 Soluble
167 V H 0.0000 Buried
168 S H -0.4534 Soluble
169 W H 0.0000 Buried
170 N H -0.6174 Soluble
171 S H -0.6744 Soluble
172 G H -0.5819 Soluble
173 A H -0.2160 Soluble
174 L H 0.0798 Aggregation-prone
175 T H -0.1380 Soluble
176 S H -0.1239 Soluble
177 G H -0.1436 Soluble
178 V H 0.2363 Aggregation-prone
179 H H -0.2389 Soluble
180 T H 0.0847 Aggregation-prone
181 F H 0.0000 Buried
182 P H -0.1104 Soluble
183 A H 0.3353 Aggregation-prone
184 V H 0.6457 Aggregation-prone
185 L H 1.3562 Aggregation-prone
186 Q H 0.2978 Aggregation-prone
187 S H -0.0705 Soluble
188 S H -0.1749 Soluble
189 G H 0.1100 Aggregation-prone
190 L H 0.3277 Aggregation-prone
191 Y H 0.5758 Aggregation-prone
192 S H 0.3577 Aggregation-prone
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.0925 Soluble
199 V H 0.0000 Buried
200 P H -0.5066 Soluble
201 S H -0.5556 Soluble
202 S H -0.7278 Soluble
203 S H -0.6951 Soluble
204 L H -0.8763 Soluble
205 G H -1.1485 Soluble
206 T H -0.8712 Soluble
207 Q H -1.4982 Soluble
208 T H -1.1946 Soluble
209 Y H 0.0000 Buried
210 I H -1.0984 Soluble
211 C H 0.0000 Buried
212 N H -1.4980 Soluble
213 V H 0.0000 Buried
214 N H -1.7415 Soluble
215 H H 0.0000 Buried
216 K H -2.6569 Soluble
217 P H -1.5188 Soluble
218 S H -1.7894 Soluble
219 N H -2.5023 Soluble
220 T H -1.9654 Soluble
221 K H -2.5772 Soluble
222 V H -1.7399 Soluble
223 D H -2.4595 Soluble
224 K H -1.8863 Soluble
225 K H -2.1792 Soluble
226 V H 0.0000 Buried
227 E H -2.8714 Soluble
228 P H -2.1623 Soluble
229 K H -2.4625 Soluble
230 S H -1.1743 Soluble
231 C H -0.0952 Soluble
1 D L -2.2833 Soluble
2 I L 0.0000 Buried
3 Q L -2.2357 Soluble
4 M L 0.0000 Buried
5 T L -1.4707 Soluble
6 Q L 0.0000 Buried
7 S L -0.8464 Soluble
8 P L -0.4351 Soluble
9 A L -0.6267 Soluble
10 T L -0.6671 Soluble
11 L L -0.2788 Soluble
12 S L -0.4097 Soluble
13 L L -0.8415 Soluble
14 S L -1.4697 Soluble
15 P L -1.8310 Soluble
16 G L -1.8807 Soluble
17 E L -2.5712 Soluble
18 R L -2.6732 Soluble
19 A L 0.0000 Buried
20 T L -0.7027 Soluble
21 L L 0.0000 Buried
22 S L -0.8787 Soluble
23 C L 0.0000 Buried
24 R L -2.8311 Soluble
25 A L 0.0000 Buried
26 S L -1.8192 Soluble
27 Q L -1.7836 Soluble
28 S L -1.0561 Soluble
29 V L 0.0000 Buried
36 S L -0.4202 Soluble
37 S L -0.0802 Soluble
38 Y L 0.4046 Aggregation-prone
39 L L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -1.0981 Soluble
45 K L -1.3756 Soluble
46 P L -1.2140 Soluble
47 G L -1.3051 Soluble
48 Q L -1.9223 Soluble
49 A L -1.3932 Soluble
50 P L 0.0000 Buried
51 R L -1.4935 Soluble
52 L L -0.5256 Soluble
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.3578 Soluble
56 D L -0.7339 Soluble
57 A L 0.0000 Buried
65 S L -0.9873 Soluble
66 N L -1.4341 Soluble
67 R L -1.7808 Soluble
68 A L -0.9693 Soluble
69 T L -0.6445 Soluble
70 G L -0.6619 Soluble
71 I L -0.5530 Soluble
72 P L -0.4304 Soluble
74 A L -0.4111 Soluble
75 R L -0.6946 Soluble
76 F L 0.0000 Buried
77 S L -0.6757 Soluble
78 G L -0.7625 Soluble
79 S L -0.8188 Soluble
80 G L -1.0548 Soluble
83 S L -0.9809 Soluble
84 G L -1.0275 Soluble
85 T L -1.7582 Soluble
86 D L -2.3616 Soluble
87 F L 0.0000 Buried
88 T L -0.8033 Soluble
89 L L 0.0000 Buried
90 T L -0.6144 Soluble
91 I L 0.0000 Buried
92 S L -1.5800 Soluble
93 S L -1.5945 Soluble
94 L L 0.0000 Buried
95 E L -2.1781 Soluble
96 P L -2.4211 Soluble
97 E L -2.7342 Soluble
98 D L 0.0000 Buried
99 F L -1.4542 Soluble
100 A L 0.0000 Buried
101 V L -0.7592 Soluble
102 Y L 0.0000 Buried
103 Y L -0.5335 Soluble
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 R L 0.0000 Buried
108 S L -0.3584 Soluble
109 N L -1.0792 Soluble
113 W L -0.0208 Soluble
114 P L 0.0000 Buried
115 M L -0.3038 Soluble
116 Y L 0.0000 Buried
117 T L -0.9086 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 Q L -2.0362 Soluble
121 G L -1.0564 Soluble
122 T L 0.0000 Buried
123 K L -1.7122 Soluble
124 L L 0.0000 Buried
125 E L -0.8418 Soluble
126 I L 0.0000 Buried
127 K L -1.0661 Soluble
128 R L -0.7036 Soluble
129 T L 0.3340 Aggregation-prone
130 V L 1.1439 Aggregation-prone
131 A L 0.4186 Aggregation-prone
132 A L -0.0420 Soluble
133 P L -0.1596 Soluble
134 S L -0.1896 Soluble
135 V L 0.0000 Buried
136 F L 0.1305 Aggregation-prone
137 I L 0.0884 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.6963 Soluble
140 P L -0.7068 Soluble
141 S L 0.0000 Buried
142 D L -2.9153 Soluble
143 E L -2.5010 Soluble
144 Q L 0.0000 Buried
145 L L -2.0893 Soluble
146 K L -2.6332 Soluble
147 S L -1.6163 Soluble
148 G L -1.0182 Soluble
149 T L -0.7424 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -1.1116 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.0221 Soluble
162 R L -1.8139 Soluble
163 E L -2.6079 Soluble
164 A L -2.0042 Soluble
165 K L -2.2322 Soluble
166 V L 0.0000 Buried
167 Q L -0.7226 Soluble
168 W L 0.0000 Buried
169 K L -0.6841 Soluble
170 V L 0.0000 Buried
171 D L -1.8404 Soluble
172 N L -1.5234 Soluble
173 A L -0.2688 Soluble
174 L L 0.6963 Aggregation-prone
175 Q L -0.1002 Soluble
176 S L -0.5172 Soluble
177 G L -0.9407 Soluble
178 N L -0.9129 Soluble
179 S L -1.3205 Soluble
180 Q L -1.7497 Soluble
181 E L -1.9991 Soluble
182 S L 0.0000 Buried
183 V L 0.0021 Aggregation-prone
184 T L -0.6828 Soluble
185 E L -1.7116 Soluble
186 Q L 0.0000 Buried
187 D L -2.6026 Soluble
188 S L -2.5442 Soluble
189 K L -3.1478 Soluble
190 D L -2.7111 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.8443 Soluble
199 L L 0.0000 Buried
200 T L -0.3717 Soluble
201 L L -0.6890 Soluble
202 S L -1.0349 Soluble
203 K L -2.0004 Soluble
204 A L -1.8212 Soluble
205 D L -2.7829 Soluble
206 Y L 0.0000 Buried
207 E L -3.6846 Soluble
208 K L -3.6505 Soluble
209 H L -3.1451 Soluble
210 K L -3.3757 Soluble
211 V L -1.7339 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.9340 Soluble
216 V L 0.0000 Buried
217 T L -1.1986 Soluble
218 H L 0.0000 Buried
219 Q L -1.5367 Soluble
220 G L -0.2792 Soluble
221 L L -0.2211 Soluble
222 S L -0.5061 Soluble
223 S L -0.4755 Soluble
224 P L -0.5957 Soluble
225 V L -0.0973 Soluble
226 T L -0.5200 Soluble
227 K L -0.6695 Soluble
228 S L -0.5973 Soluble
229 F L -1.1940 Soluble
230 N L -2.3194 Soluble
231 R L -2.7721 Soluble
232 G L -2.2801 Soluble
233 E L -2.4867 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7163 1.7928 CSV PDB
4.5 -0.7603 1.751 CSV PDB
5.0 -0.8126 1.7033 CSV PDB
5.5 -0.8636 1.6533 CSV PDB
6.0 -0.9018 1.6025 CSV PDB
6.5 -0.9188 1.5516 CSV PDB
7.0 -0.9137 1.5467 CSV PDB
7.5 -0.893 1.5458 CSV PDB
8.0 -0.8634 1.5455 CSV PDB
8.5 -0.8272 1.5454 CSV PDB
9.0 -0.7844 1.6307 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH