Zeripatamig / 8rp8

A4DAB307_8rp8_H_L | Source PDB: 8rp8 | Chains: H, L | Done
99.55
Yes
10 Å
Yes
Avg A4D Score
-0.6933
Max Score
1.082
Min Score
-3.5459
Total Score
-294.64
Score CDR
-0.3547
Score non-CDR
-0.7324

Calculation Parameters

Chain Sequence(s) EVQLLESGGGLVQPGGSLRLSCAASGFTFSSYAMSWVRQAPGKGLEWVSAISGSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKSYGAFDYWGQGTLVTVSSASTKGPSVFPLAPSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKDIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYAASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQMHPRAPKTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.5459
Maximum Score 1.082
Average Score -0.6933
Total Score -294.6368

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.1678 Soluble
2 V H -1.4011 Soluble
3 Q H -1.4802 Soluble
4 L H 0.0000 Buried
5 L H 0.4011 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.3767 Soluble
8 G H -0.9739 Soluble
9 G H -0.5372 Soluble
11 G H -0.2530 Soluble
12 L H -0.0651 Soluble
13 V H -0.6148 Soluble
14 Q H -1.5763 Soluble
15 P H -1.4858 Soluble
16 G H -1.4262 Soluble
17 G H -1.0926 Soluble
18 S H -1.2241 Soluble
19 L H -1.1711 Soluble
20 R H -2.1240 Soluble
21 L H 0.0000 Buried
22 S H -0.4711 Soluble
23 C H 0.0000 Buried
24 A H -0.3145 Soluble
25 A H 0.0000 Buried
26 S H -1.0277 Soluble
27 G H -1.2657 Soluble
28 F H -0.4805 Soluble
29 T H -0.2120 Soluble
30 F H 0.0000 Buried
35 S H -0.5164 Soluble
36 S H -0.0772 Soluble
37 Y H 0.4384 Aggregation-prone
38 A H 0.1408 Aggregation-prone
39 M H 0.0000 Buried
40 S H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.8837 Soluble
45 A H -1.2168 Soluble
46 P H -1.2868 Soluble
47 G H -1.5493 Soluble
48 K H -2.3945 Soluble
49 G H -1.6813 Soluble
50 L H 0.0000 Buried
51 E H -1.1421 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 A H 0.0000 Buried
56 I H 0.0000 Buried
57 S H -0.1872 Soluble
58 G H -0.3018 Soluble
59 S H -0.6479 Soluble
62 G H -0.7187 Soluble
63 G H -0.7076 Soluble
64 S H -0.3385 Soluble
65 T H 0.0651 Aggregation-prone
66 Y H 0.2192 Aggregation-prone
67 Y H -0.6253 Soluble
68 A H -1.3001 Soluble
69 D H -2.4057 Soluble
70 S H -1.7382 Soluble
71 V H 0.0000 Buried
72 K H -2.5026 Soluble
74 G H -1.7205 Soluble
75 R H -1.4063 Soluble
76 F H 0.0000 Buried
77 T H -0.7807 Soluble
78 I H 0.0000 Buried
79 S H -0.4849 Soluble
80 R H -1.0279 Soluble
81 D H -1.5836 Soluble
82 N H -1.7947 Soluble
83 S H -1.6294 Soluble
84 K H -2.4541 Soluble
85 N H -1.9678 Soluble
86 T H -1.1340 Soluble
87 L H 0.0000 Buried
88 Y H -0.5579 Soluble
89 L H 0.0000 Buried
90 Q H -1.2941 Soluble
91 M H 0.0000 Buried
92 N H -1.3563 Soluble
93 S H -1.1951 Soluble
94 L H 0.0000 Buried
95 R H -2.2939 Soluble
96 A H -1.6615 Soluble
97 E H -2.2414 Soluble
98 D H 0.0000 Buried
99 T H -0.7047 Soluble
100 A H 0.0000 Buried
101 V H -0.0368 Soluble
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 K H 0.0000 Buried
107 S H 0.1940 Aggregation-prone
108 Y H 0.1860 Aggregation-prone
109 G H 0.0000 Buried
114 A H 0.2014 Aggregation-prone
115 F H 0.0000 Buried
116 D H -0.3709 Soluble
117 Y H -0.1903 Soluble
118 W H -0.2614 Soluble
119 G H 0.0000 Buried
120 Q H -1.3875 Soluble
121 G H 0.0000 Buried
122 T H -0.0534 Soluble
123 L H 0.2247 Aggregation-prone
124 V H 0.0000 Buried
125 T H -0.4333 Soluble
126 V H 0.0000 Buried
127 S H -0.7482 Soluble
128 S H -0.7113 Soluble
129 A H -0.4242 Soluble
130 S H -0.5292 Soluble
131 T H -0.6178 Soluble
132 K H -1.1408 Soluble
133 G H -1.3621 Soluble
134 P H 0.0000 Buried
135 S H -0.4054 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.1599 Soluble
139 L H 0.0000 Buried
140 A H -1.2103 Soluble
141 P H 0.0000 Buried
142 S H -0.7502 Soluble
143 G H -0.6395 Soluble
144 T H -0.4744 Soluble
145 A H -0.2507 Soluble
146 A H 0.0000 Buried
147 L H 0.0000 Buried
148 G H 0.0000 Buried
149 C H 0.0000 Buried
150 L H 0.0000 Buried
151 V H 0.0000 Buried
152 K H 0.0000 Buried
153 D H -0.3705 Soluble
154 Y H 0.0000 Buried
155 F H 0.0000 Buried
156 P H 0.0000 Buried
157 E H -0.6980 Soluble
158 P H -0.8217 Soluble
159 V H -0.6986 Soluble
160 T H -0.5589 Soluble
161 V H -0.1080 Soluble
162 S H -0.3424 Soluble
163 W H 0.0000 Buried
164 N H -0.7238 Soluble
165 S H -0.6043 Soluble
166 G H -0.5168 Soluble
167 A H -0.2470 Soluble
168 L H -0.0327 Soluble
169 T H -0.1855 Soluble
170 S H -0.1895 Soluble
171 G H -0.2718 Soluble
172 V H 0.1617 Aggregation-prone
173 H H -0.2484 Soluble
174 T H -0.1467 Soluble
175 F H 0.0000 Buried
176 P H -0.2439 Soluble
177 A H 0.2162 Aggregation-prone
178 V H 0.6075 Aggregation-prone
179 L H 1.0227 Aggregation-prone
180 Q H 0.2210 Aggregation-prone
181 S H -0.1975 Soluble
182 S H -0.2542 Soluble
183 G H -0.0198 Soluble
184 L H 0.0653 Aggregation-prone
185 Y H 0.3708 Aggregation-prone
186 S H 0.0000 Buried
187 L H 0.0000 Buried
188 S H 0.0000 Buried
189 S H 0.0000 Buried
190 V H 0.0000 Buried
191 V H 0.0000 Buried
192 T H -0.1361 Soluble
193 V H 0.0000 Buried
194 P H -0.5887 Soluble
195 S H -0.5522 Soluble
196 S H -0.6225 Soluble
197 S H -0.6262 Soluble
198 L H -0.8051 Soluble
199 G H -0.9902 Soluble
200 T H -0.7192 Soluble
201 Q H -1.2828 Soluble
202 T H -1.1709 Soluble
203 Y H 0.0000 Buried
204 I H -1.2395 Soluble
205 C H 0.0000 Buried
206 N H -1.3606 Soluble
207 V H 0.0000 Buried
208 N H -1.8961 Soluble
209 H H 0.0000 Buried
210 K H -2.7783 Soluble
211 P H -1.6360 Soluble
212 S H -1.8084 Soluble
213 N H -2.5648 Soluble
214 T H -2.0914 Soluble
215 K H -2.6604 Soluble
216 V H -1.6339 Soluble
217 D H -2.1452 Soluble
218 K H -2.1457 Soluble
219 R H -2.6462 Soluble
220 V H 0.0000 Buried
221 E H -2.8483 Soluble
222 P H -1.8207 Soluble
223 K H -2.3930 Soluble
1 D L -2.4837 Soluble
2 I L 0.0000 Buried
3 Q L -2.1252 Soluble
4 M L 0.0000 Buried
5 T L -1.4696 Soluble
6 Q L 0.0000 Buried
7 S L -0.7052 Soluble
8 P L -0.6676 Soluble
9 S L -0.9470 Soluble
10 S L -1.0972 Soluble
11 L L -0.8476 Soluble
12 S L -1.3603 Soluble
13 A L 0.0000 Buried
14 S L -1.0345 Soluble
15 V L -0.0421 Soluble
16 G L -0.9412 Soluble
17 D L -2.1481 Soluble
18 R L -2.5314 Soluble
19 V L 0.0000 Buried
20 T L -0.6565 Soluble
21 I L 0.0000 Buried
22 T L -0.8449 Soluble
23 C L 0.0000 Buried
24 R L -2.7739 Soluble
25 A L 0.0000 Buried
26 S L -1.7715 Soluble
27 Q L -1.4733 Soluble
28 S L -0.9885 Soluble
29 I L 0.0000 Buried
36 S L -0.5118 Soluble
37 S L -0.1628 Soluble
38 Y L 0.4337 Aggregation-prone
39 L L 0.0000 Buried
40 N L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.6635 Soluble
46 P L -1.3831 Soluble
47 G L -1.6308 Soluble
48 K L -2.4507 Soluble
49 A L -1.5274 Soluble
50 P L 0.0000 Buried
51 K L -1.4409 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.7249 Aggregation-prone
56 A L 0.3987 Aggregation-prone
57 A L 0.0000 Buried
65 S L -0.0831 Soluble
66 S L 0.2371 Aggregation-prone
67 L L 0.4841 Aggregation-prone
68 Q L -0.1974 Soluble
69 S L -0.4120 Soluble
70 G L -0.4948 Soluble
71 V L -0.3309 Soluble
72 P L -0.3036 Soluble
74 S L -0.4076 Soluble
75 R L -0.7447 Soluble
76 F L 0.0000 Buried
77 S L -0.3019 Soluble
78 G L -0.1811 Soluble
79 S L -0.6958 Soluble
80 G L -1.0831 Soluble
83 S L -0.7944 Soluble
84 G L -1.0577 Soluble
85 T L -1.6468 Soluble
86 D L -2.3240 Soluble
87 F L 0.0000 Buried
88 T L -0.7947 Soluble
89 L L 0.0000 Buried
90 T L -0.6591 Soluble
91 I L 0.0000 Buried
92 S L -1.5315 Soluble
93 S L -1.4018 Soluble
94 L L 0.0000 Buried
95 Q L -1.2094 Soluble
96 P L -1.5166 Soluble
97 E L -1.6058 Soluble
98 D L 0.0000 Buried
99 F L 0.0000 Buried
100 A L 0.0000 Buried
101 T L -1.2119 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 M L -0.0574 Soluble
108 H L -0.7094 Soluble
109 P L -1.0396 Soluble
113 R L -2.1269 Soluble
114 A L -1.7100 Soluble
115 P L -1.1649 Soluble
116 K L -0.8101 Soluble
117 T L -0.8110 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 Q L -1.8032 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -2.1895 Soluble
124 V L 0.0000 Buried
125 E L -1.9317 Soluble
126 I L 0.0000 Buried
127 K L -1.4201 Soluble
128 R L -0.5950 Soluble
129 T L 0.2799 Aggregation-prone
130 V L 1.0820 Aggregation-prone
131 A L 0.2983 Aggregation-prone
132 A L 0.0765 Aggregation-prone
133 P L 0.0000 Buried
134 S L -0.1130 Soluble
135 V L 0.0000 Buried
136 F L -0.0927 Soluble
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.6994 Soluble
140 P L 0.0000 Buried
141 S L -1.6733 Soluble
142 D L -2.7642 Soluble
143 E L -2.5288 Soluble
144 Q L 0.0000 Buried
145 L L -2.1724 Soluble
146 K L -2.7482 Soluble
147 S L -1.7232 Soluble
148 G L -1.4062 Soluble
149 T L -1.0014 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.7626 Soluble
159 F L 0.0000 Buried
160 Y L -0.8039 Soluble
161 P L -1.4957 Soluble
162 R L -2.9691 Soluble
163 E L -3.2637 Soluble
164 A L -2.4137 Soluble
165 K L -2.5704 Soluble
166 V L 0.0000 Buried
167 Q L -0.6813 Soluble
168 W L 0.0000 Buried
169 K L -0.5200 Soluble
170 V L 0.0000 Buried
171 D L -1.9342 Soluble
172 N L -1.3416 Soluble
173 A L -0.2298 Soluble
174 L L 0.8429 Aggregation-prone
175 Q L 0.0047 Aggregation-prone
176 S L -0.4241 Soluble
177 G L -0.8695 Soluble
178 N L -0.7835 Soluble
179 S L -0.9773 Soluble
180 Q L -1.1529 Soluble
181 E L -1.3766 Soluble
182 S L -0.5312 Soluble
183 V L -0.5537 Soluble
184 T L -0.9471 Soluble
185 E L -1.9446 Soluble
186 Q L 0.0000 Buried
187 D L -1.9814 Soluble
188 S L -2.0791 Soluble
189 K L -2.5238 Soluble
190 D L -1.6165 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.5378 Soluble
199 L L 0.0000 Buried
200 T L -0.4117 Soluble
201 L L -0.6404 Soluble
202 S L -1.0155 Soluble
203 K L -2.0887 Soluble
204 A L -1.8327 Soluble
205 D L -2.3844 Soluble
206 Y L 0.0000 Buried
207 E L -3.4745 Soluble
208 K L -3.5459 Soluble
209 H L -3.0301 Soluble
210 K L -3.1547 Soluble
211 V L -1.6308 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -1.1121 Soluble
216 V L 0.0000 Buried
217 T L -1.2837 Soluble
218 H L 0.0000 Buried
219 Q L -1.5903 Soluble
220 G L -0.1951 Soluble
221 L L -0.1172 Soluble
222 S L -0.4343 Soluble
223 S L -0.3764 Soluble
224 P L -0.5620 Soluble
225 V L -0.0354 Soluble
226 T L -0.6653 Soluble
227 K L -1.0545 Soluble
228 S L -0.7603 Soluble
229 F L -1.0449 Soluble
230 N L -1.9329 Soluble
231 R L -2.5954 Soluble
232 G L -2.2541 Soluble
233 E L -2.7136 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.8015 1.6586 CSV PDB
4.5 -0.8414 1.6144 CSV PDB
5.0 -0.8873 1.5491 CSV PDB
5.5 -0.9307 1.4739 CSV PDB
6.0 -0.9613 1.4005 CSV PDB
6.5 -0.9699 1.3447 CSV PDB
7.0 -0.9561 1.3108 CSV PDB
7.5 -0.9273 1.2939 CSV PDB
8.0 -0.8901 1.2873 CSV PDB
8.5 -0.8465 1.285 CSV PDB
9.0 -0.7971 1.2842 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH