Ebribafusp / 7ue9

A4DAB096_7ue9_H_L | Source PDB: 7ue9 | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6495
Max Score
1.3223
Min Score
-3.4419
Total Score
-280.59
Score CDR
-0.1093
Score non-CDR
-0.7092

Calculation Parameters

Chain Sequence(s) VQLVQSGAEVKKPGASVKVSCKASGYTFTNYYINWVRQAPGQGLEWMGVINPYSGGTSYNQKFKGRVTMTVDTSTSTAYMELSSLRSEDTAVYFCSSPYWGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESKYGDVVMTQSPLSLPVTLGQPASISCKSSQSLLDSDGKTYLNWFQQRPGQSPRRLIYLVSKLDSGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCWQGTHFPRTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.4419
Maximum Score 1.3223
Average Score -0.6495
Total Score -280.5907

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V H 1.1490 Aggregation-prone
3 Q H -0.5765 Soluble
4 L H 0.0000 Buried
5 V H -0.3333 Soluble
6 Q H 0.0000 Buried
7 S H -0.6572 Soluble
8 G H -0.5419 Soluble
9 A H -0.0049 Soluble
11 E H -0.2219 Soluble
12 V H 0.1801 Aggregation-prone
13 K H -1.1364 Soluble
14 K H -2.1501 Soluble
15 P H -1.8019 Soluble
16 G H -1.3912 Soluble
17 A H -1.0161 Soluble
18 S H -1.2584 Soluble
19 V H 0.0000 Buried
20 K H -1.9824 Soluble
21 V H 0.0000 Buried
22 S H -0.6602 Soluble
23 C H 0.0000 Buried
24 K H -1.3407 Soluble
25 A H 0.0000 Buried
26 S H -0.3899 Soluble
27 G H 0.0103 Aggregation-prone
28 Y H 0.1007 Aggregation-prone
29 T H -0.3552 Soluble
30 F H 0.0000 Buried
35 T H 0.2024 Aggregation-prone
36 N H -0.4316 Soluble
37 Y H 0.7656 Aggregation-prone
38 Y H 1.3223 Aggregation-prone
39 I H 0.0000 Buried
40 N H 0.1874 Aggregation-prone
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H -0.3270 Soluble
44 Q H -0.5858 Soluble
45 A H -0.9534 Soluble
46 P H -0.8060 Soluble
47 G H -1.2240 Soluble
48 Q H -1.7414 Soluble
49 G H -1.0994 Soluble
50 L H -0.4749 Soluble
51 E H -0.5897 Soluble
52 W H 0.0000 Buried
53 M H 0.0000 Buried
54 G H 0.0000 Buried
55 V H 0.0000 Buried
56 I H 0.0000 Buried
57 N H 0.4046 Aggregation-prone
58 P H 0.0000 Buried
59 Y H 1.0255 Aggregation-prone
62 S H 0.0817 Aggregation-prone
63 G H -0.2194 Soluble
64 G H -0.2985 Soluble
65 T H -0.2044 Soluble
66 S H -0.2278 Soluble
67 Y H -0.6784 Soluble
68 N H -1.2545 Soluble
69 Q H -2.5072 Soluble
70 K H -2.8670 Soluble
71 F H 0.0000 Buried
72 K H -2.9314 Soluble
74 G H -1.8455 Soluble
75 R H -1.6270 Soluble
76 V H 0.0000 Buried
77 T H -0.8586 Soluble
78 M H 0.0000 Buried
79 T H -0.2795 Soluble
80 V H -0.2445 Soluble
81 D H -1.0436 Soluble
82 T H -0.3975 Soluble
83 S H -0.5198 Soluble
84 T H -0.6783 Soluble
85 S H -0.7367 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.5910 Soluble
89 M H 0.0000 Buried
90 E H -1.5205 Soluble
91 L H 0.0000 Buried
92 S H -1.0138 Soluble
93 S H -1.0046 Soluble
94 L H 0.0000 Buried
95 R H -2.1422 Soluble
96 S H -1.9337 Soluble
97 E H -2.2412 Soluble
98 D H 0.0000 Buried
99 T H -0.7044 Soluble
100 A H 0.0000 Buried
101 V H 0.3345 Aggregation-prone
102 Y H 0.0000 Buried
103 F H 0.0000 Buried
104 C H 0.0000 Buried
105 S H 0.0000 Buried
106 S H 0.0000 Buried
116 P H 0.5235 Aggregation-prone
117 Y H 0.1514 Aggregation-prone
118 W H 0.0000 Buried
119 G H 0.0000 Buried
120 Q H -1.3850 Soluble
121 G H -0.5860 Soluble
122 T H 0.0000 Buried
123 L H 0.3730 Aggregation-prone
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -1.1318 Soluble
128 S H -0.9366 Soluble
129 A H -0.5785 Soluble
130 S H -0.6070 Soluble
131 T H -0.6002 Soluble
132 K H -1.2324 Soluble
133 G H -1.4380 Soluble
134 P H -0.6718 Soluble
135 S H -0.3848 Soluble
136 V H 0.0000 Buried
137 F H -0.7109 Soluble
138 P H -1.0444 Soluble
139 L H 0.0000 Buried
140 A H -0.5279 Soluble
141 P H -0.1917 Soluble
142 C H -0.4620 Soluble
143 S H -0.3359 Soluble
144 R H -0.8986 Soluble
145 S H -0.6479 Soluble
146 T H -1.0842 Soluble
147 S H -1.2182 Soluble
148 E H -2.1406 Soluble
149 S H -1.2726 Soluble
150 T H -0.8924 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H 0.0000 Buried
159 D H -0.5032 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H -0.6857 Soluble
163 E H -0.6490 Soluble
164 P H -1.0089 Soluble
165 V H -0.9495 Soluble
166 T H -0.7765 Soluble
167 V H -0.3650 Soluble
168 S H -0.5706 Soluble
169 W H 0.0000 Buried
170 N H -1.0710 Soluble
171 S H -0.8026 Soluble
172 G H -0.5922 Soluble
173 A H -0.2568 Soluble
174 L H -0.0517 Soluble
175 T H -0.1643 Soluble
176 S H -0.1622 Soluble
177 G H -0.1820 Soluble
178 V H 0.1611 Aggregation-prone
179 H H -0.3148 Soluble
180 T H 0.0102 Aggregation-prone
181 F H 0.0000 Buried
182 P H -0.5562 Soluble
183 A H -0.0911 Soluble
184 V H 0.2056 Aggregation-prone
185 L H 0.8510 Aggregation-prone
186 Q H 0.0877 Aggregation-prone
187 S H -0.1587 Soluble
188 S H -0.2494 Soluble
189 G H -0.0696 Soluble
190 L H -0.0726 Soluble
191 Y H 0.0000 Buried
192 S H 0.0000 Buried
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.1137 Soluble
199 V H 0.0000 Buried
200 P H -0.7212 Soluble
201 S H -0.8543 Soluble
202 S H -0.8575 Soluble
203 S H 0.0000 Buried
204 L H -1.0428 Soluble
205 G H -1.2072 Soluble
206 T H -1.1023 Soluble
207 K H -1.8684 Soluble
208 T H -1.5860 Soluble
209 Y H 0.0000 Buried
210 T H -1.7871 Soluble
211 C H 0.0000 Buried
212 N H -1.8383 Soluble
213 V H 0.0000 Buried
214 D H -2.4904 Soluble
215 H H 0.0000 Buried
216 K H -2.9051 Soluble
217 P H -1.7241 Soluble
218 S H -1.9002 Soluble
219 N H -2.6584 Soluble
220 T H -2.2401 Soluble
221 K H -2.8742 Soluble
222 V H -1.8823 Soluble
223 D H -2.9008 Soluble
224 K H -2.3715 Soluble
225 R H -2.8323 Soluble
226 V H 0.0000 Buried
227 E H -2.1050 Soluble
228 S H -1.3096 Soluble
229 K H -1.7048 Soluble
230 Y H -0.1828 Soluble
231 G H -0.5772 Soluble
1 D L -1.1071 Soluble
2 V L 0.0554 Aggregation-prone
3 V L 0.8978 Aggregation-prone
4 M L 0.0000 Buried
5 T L -0.1952 Soluble
6 Q L 0.0000 Buried
7 S L -0.0938 Soluble
8 P L 0.4227 Aggregation-prone
9 L L 1.2261 Aggregation-prone
10 S L 0.3046 Aggregation-prone
11 L L 0.1599 Aggregation-prone
12 P L -0.6159 Soluble
13 V L 0.0000 Buried
14 T L -0.8467 Soluble
15 L L -0.3686 Soluble
16 G L -1.2606 Soluble
17 Q L -1.8607 Soluble
18 P L -1.8272 Soluble
19 A L 0.0000 Buried
20 S L -0.7488 Soluble
21 I L 0.0000 Buried
22 S L -0.7773 Soluble
23 C L 0.0000 Buried
24 K L -1.8853 Soluble
25 S L 0.0000 Buried
26 S L -0.8424 Soluble
27 Q L -1.4197 Soluble
28 S L -0.7741 Soluble
29 L L 0.0000 Buried
30 L L 0.3218 Aggregation-prone
31 D L -0.9429 Soluble
32 S L -1.3105 Soluble
34 D L -2.3689 Soluble
35 G L -1.6877 Soluble
36 K L -1.5427 Soluble
37 T L -0.3119 Soluble
38 Y L 0.2115 Aggregation-prone
39 L L 0.0000 Buried
40 N L -0.0490 Soluble
41 W L 0.0000 Buried
42 F L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 R L -1.7074 Soluble
46 P L -1.3682 Soluble
47 G L -1.3201 Soluble
48 Q L -1.7719 Soluble
49 S L -1.3213 Soluble
50 P L 0.0000 Buried
51 R L -1.0964 Soluble
52 R L -0.5454 Soluble
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.0701 Aggregation-prone
56 L L 0.0828 Aggregation-prone
57 V L 0.0000 Buried
65 S L -0.6356 Soluble
66 K L -1.0215 Soluble
67 L L -0.6990 Soluble
68 D L 0.0000 Buried
69 S L -0.5430 Soluble
70 G L -0.7712 Soluble
71 V L 0.0000 Buried
72 P L -1.1211 Soluble
74 D L -2.0020 Soluble
75 R L -2.0526 Soluble
76 F L 0.0000 Buried
77 S L -1.2367 Soluble
78 G L -0.6964 Soluble
79 S L -0.8531 Soluble
80 G L -1.0338 Soluble
83 S L -0.7348 Soluble
84 G L -0.7870 Soluble
85 T L -1.5026 Soluble
86 D L -2.2279 Soluble
87 F L 0.0000 Buried
88 T L -1.0155 Soluble
89 L L 0.0000 Buried
90 K L -1.5867 Soluble
91 I L 0.0000 Buried
92 S L -2.2023 Soluble
93 R L -2.7286 Soluble
94 V L 0.0000 Buried
95 E L -1.8266 Soluble
96 A L -0.9650 Soluble
97 E L -2.0821 Soluble
98 D L 0.0000 Buried
99 V L -0.4635 Soluble
100 G L -0.7462 Soluble
101 V L 0.0511 Aggregation-prone
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 W L 0.1962 Aggregation-prone
106 Q L 0.0000 Buried
107 G L 0.3990 Aggregation-prone
108 T L 0.0000 Buried
109 H L 0.2309 Aggregation-prone
114 F L 1.1672 Aggregation-prone
115 P L 0.1132 Aggregation-prone
116 R L 0.2279 Aggregation-prone
117 T L 0.2644 Aggregation-prone
118 F L 0.2831 Aggregation-prone
119 G L 0.0000 Buried
120 G L -0.2724 Soluble
121 G L -0.1600 Soluble
122 T L 0.0000 Buried
123 K L -0.1025 Soluble
124 V L 0.0000 Buried
125 E L 0.0000 Buried
126 I L -0.5897 Soluble
127 K L -1.3668 Soluble
128 R L -1.1323 Soluble
129 T L -0.1857 Soluble
130 V L 0.3141 Aggregation-prone
131 A L 0.1082 Aggregation-prone
132 A L -0.1184 Soluble
133 P L 0.0000 Buried
134 S L -0.1384 Soluble
135 V L 0.0000 Buried
136 F L 0.1672 Aggregation-prone
137 I L 0.1756 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.3651 Soluble
140 P L -0.7444 Soluble
141 S L -1.8227 Soluble
142 D L -3.2090 Soluble
143 E L -3.1034 Soluble
144 Q L 0.0000 Buried
145 L L -2.2914 Soluble
146 K L -2.8959 Soluble
147 S L -1.7565 Soluble
148 G L -1.3238 Soluble
149 T L -1.0001 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.8673 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.5451 Soluble
162 R L -2.5933 Soluble
163 E L -2.9935 Soluble
164 A L -2.1920 Soluble
165 K L -2.3079 Soluble
166 V L -0.9446 Soluble
167 Q L -0.3842 Soluble
168 W L 0.0000 Buried
169 K L -0.4594 Soluble
170 V L 0.0000 Buried
171 D L -1.8610 Soluble
172 N L -1.4570 Soluble
173 A L -0.1762 Soluble
174 L L 0.8299 Aggregation-prone
175 Q L -0.0196 Soluble
176 S L -0.4016 Soluble
177 G L -0.8773 Soluble
178 N L -0.8160 Soluble
179 S L -1.0813 Soluble
180 Q L -1.4077 Soluble
181 E L -1.9510 Soluble
182 S L -0.8895 Soluble
183 V L -0.8072 Soluble
184 T L -1.1658 Soluble
185 E L -2.3334 Soluble
186 Q L -1.8265 Soluble
187 D L -2.0002 Soluble
188 S L -1.7059 Soluble
189 K L -2.2575 Soluble
190 D L -1.7353 Soluble
191 S L -1.7294 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.6326 Soluble
199 L L 0.0000 Buried
200 T L -0.4092 Soluble
201 L L -0.5964 Soluble
202 S L -0.9578 Soluble
203 K L -1.9259 Soluble
204 A L -1.7149 Soluble
205 D L -2.3716 Soluble
206 Y L 0.0000 Buried
207 E L -3.2007 Soluble
208 K L -3.4419 Soluble
209 H L -2.8862 Soluble
210 K L -3.1625 Soluble
211 V L -1.3446 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.7690 Soluble
216 V L 0.0000 Buried
217 T L -1.1773 Soluble
218 H L 0.0000 Buried
219 Q L -1.7047 Soluble
220 G L -0.3940 Soluble
221 L L -0.1435 Soluble
222 S L -0.4071 Soluble
223 S L -0.3988 Soluble
224 P L -0.5124 Soluble
225 V L 0.0778 Aggregation-prone
226 T L -0.3659 Soluble
227 K L -0.5693 Soluble
228 S L -0.3880 Soluble
229 F L -0.7171 Soluble
230 N L -1.7325 Soluble
231 R L -2.3333 Soluble
232 G L -1.9009 Soluble
233 E L -2.0966 Soluble
234 C L -0.4114 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6814 3.551 CSV PDB
4.5 -0.7286 3.4758 CSV PDB
5.0 -0.7831 3.397 CSV PDB
5.5 -0.8339 3.3264 CSV PDB
6.0 -0.8684 3.2708 CSV PDB
6.5 -0.8771 3.2217 CSV PDB
7.0 -0.8598 3.1624 CSV PDB
7.5 -0.8251 3.0889 CSV PDB
8.0 -0.7807 3.0105 CSV PDB
8.5 -0.7291 2.9401 CSV PDB
9.0 -0.6705 2.8902 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH