Sutacimig / 8che

A4DAB249_8che_H_L | Source PDB: 8che | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6977
Max Score
1.3219
Min Score
-3.8911
Total Score
-297.92
Score CDR
-0.4184
Score non-CDR
-0.7306

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFTFSRYWMTWVRQAPGKGLVWVGEINPDSSTINYAPSVKGRFTISRDNAKNTLYLQMNSLRAEDTAVYYCASGVFTWGQGTLVTVSSAKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPALQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESDIVMTQTPLSSPVTLGQPASISCRSSQSLVHRQGNTYFHWLQQRPGQPPRLLIYKVSNRFSGVPDRFSGSGAGTDFTLKIRVEAEDVGVYYCSQSTHVPYTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQCNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.8911
Maximum Score 1.3219
Average Score -0.6977
Total Score -297.922

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -1.5695 Soluble
2 V H -0.0530 Soluble
3 Q H -0.6639 Soluble
4 L H 0.0000 Buried
5 V H 0.9542 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.4796 Soluble
8 G H -0.8548 Soluble
9 G H -0.4966 Soluble
11 G H -0.1921 Soluble
12 L H 0.2931 Aggregation-prone
13 V H 0.0000 Buried
14 Q H -1.5457 Soluble
15 P H -1.7213 Soluble
16 G H -1.4140 Soluble
17 G H -1.1226 Soluble
18 S H -1.3087 Soluble
19 L H -1.1443 Soluble
20 R H -2.1961 Soluble
21 L H 0.0000 Buried
22 S H -0.4714 Soluble
23 C H 0.0000 Buried
24 A H -0.2608 Soluble
25 A H 0.0000 Buried
26 S H -0.6927 Soluble
27 G H -0.6818 Soluble
28 F H -0.2672 Soluble
29 T H -0.4501 Soluble
30 F H 0.0000 Buried
35 S H -1.5462 Soluble
36 R H -1.7579 Soluble
37 Y H -0.1416 Soluble
38 W H 0.2370 Aggregation-prone
39 M H 0.0000 Buried
40 T H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0346 Aggregation-prone
44 Q H -0.3952 Soluble
45 A H -0.9190 Soluble
46 P H -0.9369 Soluble
47 G H -1.4317 Soluble
48 K H -2.1569 Soluble
49 G H -1.1724 Soluble
50 L H 0.0000 Buried
51 V H 0.6740 Aggregation-prone
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 G H 0.0000 Buried
55 E H 0.0000 Buried
56 I H 0.0000 Buried
57 N H -0.6309 Soluble
58 P H -1.3583 Soluble
59 D H -2.2086 Soluble
62 S H -1.1110 Soluble
63 S H -0.7206 Soluble
64 T H -0.1843 Soluble
65 I H 0.3821 Aggregation-prone
66 N H 0.2120 Aggregation-prone
67 Y H -0.1475 Soluble
68 A H 0.0000 Buried
69 P H -1.0028 Soluble
70 S H -0.7490 Soluble
71 V H 0.0000 Buried
72 K H -2.1545 Soluble
74 G H -1.5740 Soluble
75 R H -1.4122 Soluble
76 F H 0.0000 Buried
77 T H -0.8788 Soluble
78 I H 0.0000 Buried
79 S H -0.5235 Soluble
80 R H -1.2165 Soluble
81 D H -1.7182 Soluble
82 N H -2.0456 Soluble
83 A H -1.3487 Soluble
84 K H -2.3003 Soluble
85 N H -1.7364 Soluble
86 T H 0.0000 Buried
87 L H 0.0000 Buried
88 Y H -0.6675 Soluble
89 L H 0.0000 Buried
90 Q H -1.5093 Soluble
91 M H 0.0000 Buried
92 N H -1.3846 Soluble
93 S H -1.1619 Soluble
94 L H 0.0000 Buried
95 R H -2.3864 Soluble
96 A H -1.7774 Soluble
97 E H -2.2499 Soluble
98 D H 0.0000 Buried
99 T H -0.7180 Soluble
100 A H 0.0000 Buried
101 V H 0.1008 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 S H 0.4616 Aggregation-prone
107 G H 0.3806 Aggregation-prone
108 V H 0.8490 Aggregation-prone
115 F H 0.0000 Buried
116 T H 0.1470 Aggregation-prone
118 W H -0.1628 Soluble
119 G H 0.0000 Buried
120 Q H -1.2293 Soluble
121 G H -0.5696 Soluble
122 T H -0.1718 Soluble
123 L H 0.0737 Aggregation-prone
124 V H 0.0000 Buried
125 T H -0.4047 Soluble
126 V H 0.0000 Buried
127 S H -0.6600 Soluble
128 S H -0.6004 Soluble
129 A H -0.3494 Soluble
132 K H -1.8952 Soluble
133 G H -1.7298 Soluble
134 P H -0.6985 Soluble
135 S H -0.4854 Soluble
136 V H 0.0000 Buried
137 F H -0.7214 Soluble
138 P H -1.1486 Soluble
139 L H 0.0000 Buried
140 A H -0.3892 Soluble
141 P H -0.4233 Soluble
142 C H -0.6084 Soluble
143 S H -1.0867 Soluble
144 R H -2.0559 Soluble
145 S H -1.3211 Soluble
146 T H -1.2233 Soluble
147 S H -1.5733 Soluble
148 E H -2.3853 Soluble
149 S H -1.3126 Soluble
150 T H -0.8845 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H 0.0000 Buried
159 D H -0.4472 Soluble
160 Y H 0.0000 Buried
161 F H -0.3767 Soluble
162 P H 0.0000 Buried
163 E H -0.6310 Soluble
164 P H -0.7236 Soluble
165 V H -0.6124 Soluble
166 T H -0.5313 Soluble
167 V H -0.1882 Soluble
168 S H -0.4840 Soluble
169 W H 0.0000 Buried
170 N H -1.1340 Soluble
171 S H -0.7786 Soluble
172 G H -0.6049 Soluble
173 A H -0.2977 Soluble
174 L H -0.1518 Soluble
175 T H -0.2174 Soluble
176 S H -0.2012 Soluble
177 G H -0.2443 Soluble
178 V H 0.1275 Aggregation-prone
179 H H -0.0917 Soluble
180 T H 0.0802 Aggregation-prone
181 F H 0.0000 Buried
182 P H -0.1981 Soluble
183 A H 0.1939 Aggregation-prone
185 L H 1.3219 Aggregation-prone
186 Q H 0.2450 Aggregation-prone
187 S H -0.1782 Soluble
188 S H -0.1706 Soluble
189 G H 0.0989 Aggregation-prone
190 L H 0.1059 Aggregation-prone
191 Y H 0.4343 Aggregation-prone
192 S H 0.3447 Aggregation-prone
193 L H 0.1430 Aggregation-prone
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H 0.0000 Buried
199 V H 0.0000 Buried
200 P H -0.6649 Soluble
201 S H -0.8226 Soluble
202 S H -0.6449 Soluble
203 S H -0.5306 Soluble
204 L H -0.7062 Soluble
205 G H -1.0685 Soluble
206 T H -0.8515 Soluble
207 K H -1.3341 Soluble
208 T H -1.4527 Soluble
209 Y H 0.0000 Buried
210 T H -1.9066 Soluble
211 C H 0.0000 Buried
212 N H -1.6657 Soluble
213 V H 0.0000 Buried
214 D H -1.8608 Soluble
215 H H 0.0000 Buried
216 K H -2.7627 Soluble
217 P H -1.7295 Soluble
218 S H -2.0641 Soluble
219 N H -2.6398 Soluble
220 T H -2.1998 Soluble
221 K H -2.7148 Soluble
222 V H -1.7359 Soluble
223 D H -2.7559 Soluble
224 K H -2.4618 Soluble
225 R H -2.9146 Soluble
226 V H 0.0000 Buried
227 E H -2.4727 Soluble
228 S H -1.1694 Soluble
1 D L -1.2495 Soluble
2 I L 0.0000 Buried
3 V L 0.7788 Aggregation-prone
4 M L 0.0000 Buried
5 T L -0.7303 Soluble
6 Q L 0.0000 Buried
7 T L -0.2139 Soluble
8 P L -0.1477 Soluble
9 L L 0.6226 Aggregation-prone
10 S L 0.0273 Aggregation-prone
11 S L -0.3745 Soluble
12 P L -0.7162 Soluble
13 V L 0.0000 Buried
14 T L -1.2464 Soluble
15 L L -1.0568 Soluble
16 G L -1.5735 Soluble
17 Q L -1.9610 Soluble
18 P L -1.8807 Soluble
19 A L 0.0000 Buried
20 S L -0.8767 Soluble
21 I L 0.0000 Buried
22 S L -1.2265 Soluble
23 C L 0.0000 Buried
24 R L -2.4309 Soluble
25 S L 0.0000 Buried
26 S L -0.8407 Soluble
27 Q L -1.1430 Soluble
28 S L -0.9673 Soluble
29 L L 0.0000 Buried
30 V L -0.3046 Soluble
31 H L -1.3855 Soluble
32 R L -2.5533 Soluble
34 Q L -2.3207 Soluble
35 G L -1.6972 Soluble
36 N L -1.2283 Soluble
37 T L -0.4571 Soluble
38 Y L 0.2087 Aggregation-prone
39 F L 0.0000 Buried
40 H L 0.0000 Buried
41 W L 0.0000 Buried
42 L L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 R L -1.3486 Soluble
46 P L -1.2226 Soluble
47 G L -1.2916 Soluble
48 Q L -1.7471 Soluble
49 P L -1.1460 Soluble
50 P L 0.0000 Buried
51 R L -0.6271 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.1051 Aggregation-prone
56 K L -0.6871 Soluble
57 V L -0.4321 Soluble
65 S L -0.6783 Soluble
66 N L -0.8957 Soluble
67 R L -1.2379 Soluble
68 F L -0.2487 Soluble
69 S L -0.4789 Soluble
70 G L -0.8033 Soluble
71 V L 0.0000 Buried
72 P L -1.3675 Soluble
74 D L -2.4699 Soluble
75 R L -2.3240 Soluble
76 F L 0.0000 Buried
77 S L -1.3743 Soluble
78 G L 0.0000 Buried
79 S L -0.9123 Soluble
80 G L -1.0865 Soluble
83 A L -0.7058 Soluble
84 G L -0.8020 Soluble
85 T L -1.7022 Soluble
86 D L -2.7663 Soluble
87 F L 0.0000 Buried
88 T L -1.1924 Soluble
89 L L 0.0000 Buried
90 K L -1.8032 Soluble
91 I L 0.0000 Buried
93 R L -3.0896 Soluble
94 V L 0.0000 Buried
95 E L -2.7657 Soluble
96 A L -2.2804 Soluble
97 E L -2.6238 Soluble
98 D L 0.0000 Buried
99 V L 0.0000 Buried
100 G L 0.0000 Buried
101 V L -0.2328 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 S L 0.0000 Buried
106 Q L 0.0000 Buried
107 S L 0.3999 Aggregation-prone
108 T L 0.0000 Buried
109 H L 0.2386 Aggregation-prone
114 V L 0.9706 Aggregation-prone
115 P L 0.4871 Aggregation-prone
116 Y L 0.7935 Aggregation-prone
117 T L 0.5288 Aggregation-prone
118 F L 0.4716 Aggregation-prone
119 G L 0.0000 Buried
120 Q L -1.2853 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -0.4883 Soluble
124 L L 0.0000 Buried
125 E L -0.7522 Soluble
126 I L 0.0000 Buried
127 K L -1.2501 Soluble
128 R L -0.7417 Soluble
129 T L 0.0169 Aggregation-prone
130 V L 0.4781 Aggregation-prone
131 A L 0.0504 Aggregation-prone
132 A L -0.1142 Soluble
133 P L 0.0000 Buried
134 S L -0.4094 Soluble
135 V L 0.0000 Buried
136 F L 0.0000 Buried
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.3853 Soluble
140 P L 0.0000 Buried
141 S L -1.5559 Soluble
142 D L -2.9093 Soluble
143 E L -3.0515 Soluble
144 Q L 0.0000 Buried
145 L L -2.3004 Soluble
146 K L -2.8508 Soluble
147 S L -1.7741 Soluble
148 G L -1.2172 Soluble
149 T L -0.9061 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.8376 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.4115 Soluble
162 R L -2.3811 Soluble
163 E L -2.9508 Soluble
164 A L -2.1920 Soluble
165 K L -2.4447 Soluble
166 V L -1.2410 Soluble
167 Q L -0.7923 Soluble
168 W L 0.0000 Buried
169 K L -0.7240 Soluble
170 V L 0.0000 Buried
171 D L -2.0045 Soluble
172 N L -1.5957 Soluble
173 A L -0.3041 Soluble
174 L L 0.6645 Aggregation-prone
175 Q L 0.0695 Aggregation-prone
177 C L 0.3155 Aggregation-prone
178 N L -0.2929 Soluble
179 S L -1.0589 Soluble
180 Q L -1.7939 Soluble
181 E L -2.1988 Soluble
182 S L -1.0287 Soluble
183 V L -0.3814 Soluble
184 T L -0.7660 Soluble
185 E L -1.6936 Soluble
186 Q L 0.0000 Buried
187 D L -1.9627 Soluble
188 S L -2.3812 Soluble
189 K L -2.7410 Soluble
190 D L -1.8424 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.8481 Soluble
199 L L 0.0000 Buried
200 T L -0.4216 Soluble
201 L L -0.8121 Soluble
202 S L -1.2524 Soluble
203 K L -2.2799 Soluble
204 A L -2.1657 Soluble
205 D L -3.0394 Soluble
206 Y L 0.0000 Buried
207 E L -3.8804 Soluble
208 K L -3.8911 Soluble
209 H L -3.1885 Soluble
210 K L -3.3829 Soluble
211 V L -1.5698 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -1.0700 Soluble
216 V L 0.0000 Buried
217 T L -1.2899 Soluble
218 H L 0.0000 Buried
219 Q L -1.6888 Soluble
220 G L -0.4329 Soluble
221 L L -0.2532 Soluble
222 S L -0.4825 Soluble
223 S L -0.4389 Soluble
224 P L -0.6192 Soluble
225 V L -0.0913 Soluble
226 T L -0.6124 Soluble
227 K L -0.8689 Soluble
228 S L -0.7465 Soluble
229 F L 0.0000 Buried
230 N L -1.9490 Soluble
231 R L -2.5304 Soluble
232 G L -1.9754 Soluble
233 E L -2.2156 Soluble
234 C L -0.7049 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7084 1.6564 CSV PDB
4.5 -0.7557 1.6481 CSV PDB
5.0 -0.8116 1.6382 CSV PDB
5.5 -0.8662 1.6274 CSV PDB
6.0 -0.9086 1.6201 CSV PDB
6.5 -0.9297 1.6497 CSV PDB
7.0 -0.9278 1.6929 CSV PDB
7.5 -0.9095 1.7437 CSV PDB
8.0 -0.8813 1.7975 CSV PDB
8.5 -0.8459 1.8523 CSV PDB
9.0 -0.8037 1.907 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH