Anbenitamab / 6bhz

A4DAB016_6bhz_H_L | Source PDB: 6bhz | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.696
Max Score
1.6753
Min Score
-3.3308
Total Score
-292.34
Score CDR
-0.4897
Score non-CDR
-0.7208

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYRWGGDGFYAMDYWGQGTLVTVSSATKGPSVFPLAPTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPDIQMTQSPSSLSASVGDRVTITCRASQDVNTAVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKAAYEKHKVYAEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.3308
Maximum Score 1.6753
Average Score -0.696
Total Score -292.3354

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.1593 Soluble
2 V H -1.3907 Soluble
3 Q H -1.3765 Soluble
4 L H 0.0000 Buried
5 V H 0.7943 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.4629 Soluble
8 G H -0.9434 Soluble
9 G H -0.4903 Soluble
11 G H 0.0118 Aggregation-prone
12 L H 0.1351 Aggregation-prone
13 V H -0.5278 Soluble
14 Q H -1.5062 Soluble
15 P H -1.6589 Soluble
16 G H -1.4143 Soluble
17 G H -1.1023 Soluble
18 S H -1.1409 Soluble
19 L H -1.2012 Soluble
20 R H -2.1991 Soluble
21 L H 0.0000 Buried
22 S H -0.4261 Soluble
23 C H 0.0000 Buried
24 A H -0.0862 Soluble
25 A H -0.4092 Soluble
26 S H -0.8197 Soluble
27 G H -1.1876 Soluble
28 F H -0.8728 Soluble
30 I H 0.0000 Buried
35 K H -2.7984 Soluble
36 D H -2.8549 Soluble
37 T H 0.0000 Buried
38 Y H -0.7302 Soluble
39 I H 0.0000 Buried
40 H H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.7851 Soluble
45 A H -1.1632 Soluble
46 P H -0.9591 Soluble
47 G H -1.4771 Soluble
48 K H -2.3861 Soluble
49 G H -1.6394 Soluble
50 L H 0.0000 Buried
51 E H -1.0674 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 A H 0.0000 Buried
55 R H -0.5435 Soluble
56 I H 0.0000 Buried
57 Y H -0.5256 Soluble
58 P H 0.0000 Buried
59 T H -1.4250 Soluble
62 N H -1.1880 Soluble
63 G H -0.4719 Soluble
64 Y H 0.5117 Aggregation-prone
65 T H -0.3284 Soluble
66 R H -1.6641 Soluble
67 Y H -1.7048 Soluble
68 A H -1.7847 Soluble
69 D H -2.6487 Soluble
70 S H -1.7809 Soluble
71 V H 0.0000 Buried
72 K H -2.8019 Soluble
74 G H -1.7106 Soluble
75 R H -1.4224 Soluble
76 F H 0.0000 Buried
77 T H -1.0968 Soluble
78 I H 0.0000 Buried
79 S H -0.1642 Soluble
80 A H -0.9667 Soluble
81 D H -1.5311 Soluble
82 T H -1.7775 Soluble
83 S H -1.5293 Soluble
84 K H -2.1383 Soluble
85 N H -1.6106 Soluble
86 T H -0.7781 Soluble
87 A H 0.0000 Buried
88 Y H -0.6001 Soluble
89 L H 0.0000 Buried
90 Q H -1.4590 Soluble
91 M H 0.0000 Buried
92 N H -1.3499 Soluble
93 S H -1.2002 Soluble
94 L H 0.0000 Buried
95 R H -2.2919 Soluble
96 A H -1.7108 Soluble
97 E H -2.2498 Soluble
98 D H 0.0000 Buried
99 T H -0.6617 Soluble
100 A H 0.0000 Buried
101 V H 0.2882 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
106 R H -0.5497 Soluble
107 W H 0.0000 Buried
108 G H 0.0000 Buried
109 G H -1.0183 Soluble
110 D H -1.8466 Soluble
111 G H 0.0000 Buried
112 F H 0.7085 Aggregation-prone
113 Y H 0.7002 Aggregation-prone
114 A H 0.0000 Buried
115 M H 0.0000 Buried
116 D H -0.0780 Soluble
117 Y H -0.2947 Soluble
118 W H -0.2893 Soluble
119 G H 0.0000 Buried
120 Q H -1.3223 Soluble
121 G H -0.5441 Soluble
122 T H -0.0093 Soluble
123 L H 0.3253 Aggregation-prone
124 V H 0.0000 Buried
125 T H -0.3476 Soluble
126 V H 0.0000 Buried
127 S H -0.8694 Soluble
128 S H -0.6119 Soluble
129 A H -0.3090 Soluble
131 T H -0.6088 Soluble
132 K H -1.0540 Soluble
133 G H -1.3937 Soluble
134 P H 0.0000 Buried
135 S H -0.2590 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.1388 Soluble
139 L H 0.0000 Buried
140 A H -0.7889 Soluble
141 P H -0.4380 Soluble
142 T H -0.3022 Soluble
143 A H -0.1454 Soluble
144 A H 0.0000 Buried
145 L H 0.0000 Buried
146 G H 0.0000 Buried
147 C H 0.0000 Buried
148 L H 0.0000 Buried
149 V H 0.0000 Buried
150 K H 0.0000 Buried
151 D H -0.2918 Soluble
152 Y H 0.0000 Buried
153 F H 0.0000 Buried
154 P H 0.0000 Buried
155 E H -0.4948 Soluble
156 P H -0.8224 Soluble
157 V H -0.8632 Soluble
158 T H -0.7700 Soluble
159 V H -0.3097 Soluble
160 S H -0.4685 Soluble
161 W H 0.0000 Buried
162 N H -0.7424 Soluble
163 S H -0.6634 Soluble
164 G H -0.5410 Soluble
165 A H -0.2645 Soluble
166 L H -0.0167 Soluble
167 T H -0.1908 Soluble
168 S H -0.1922 Soluble
169 G H -0.2351 Soluble
170 V H 0.1629 Aggregation-prone
171 H H -0.3319 Soluble
172 T H -0.1809 Soluble
173 F H 0.0000 Buried
174 P H -0.2956 Soluble
175 A H 0.2691 Aggregation-prone
176 V H 0.6264 Aggregation-prone
177 L H 1.1570 Aggregation-prone
178 Q H 0.3072 Aggregation-prone
179 S H -0.1368 Soluble
180 S H -0.2043 Soluble
181 G H 0.0601 Aggregation-prone
182 L H 0.2468 Aggregation-prone
183 Y H 0.5233 Aggregation-prone
184 S H 0.0000 Buried
185 L H 0.0000 Buried
186 S H 0.0000 Buried
187 S H 0.0000 Buried
188 V H 0.0000 Buried
189 V H 0.0000 Buried
190 T H -0.1587 Soluble
191 V H 0.0000 Buried
192 P H -0.3990 Soluble
193 S H -0.4330 Soluble
194 S H -0.5364 Soluble
195 S H -0.6151 Soluble
196 L H -0.7661 Soluble
197 G H -1.1578 Soluble
198 T H -1.0254 Soluble
199 Q H -1.4601 Soluble
200 T H -1.2162 Soluble
201 Y H 0.0000 Buried
202 I H -1.1593 Soluble
203 C H 0.0000 Buried
204 N H -1.5375 Soluble
205 V H 0.0000 Buried
206 N H -2.5105 Soluble
207 H H 0.0000 Buried
208 K H -2.8968 Soluble
209 P H -1.6500 Soluble
210 S H -1.8809 Soluble
211 N H -2.6672 Soluble
212 T H -2.2384 Soluble
213 K H -2.9243 Soluble
214 V H -1.7087 Soluble
215 D H -2.4122 Soluble
216 K H -2.0355 Soluble
217 K H -2.3593 Soluble
218 V H 0.0000 Buried
219 E H -2.5156 Soluble
220 P H -1.3515 Soluble
1 D L -2.2576 Soluble
2 I L 0.0000 Buried
3 Q L -2.2932 Soluble
4 M L 0.0000 Buried
5 T L -1.4320 Soluble
6 Q L 0.0000 Buried
7 S L -0.8616 Soluble
8 P L -0.6137 Soluble
9 S L -0.8540 Soluble
10 S L -0.7229 Soluble
11 L L -0.4503 Soluble
12 S L -0.5990 Soluble
13 A L -0.8615 Soluble
14 S L -0.7156 Soluble
15 V L -0.0680 Soluble
16 G L -0.7720 Soluble
17 D L -1.7424 Soluble
18 R L -2.2744 Soluble
19 V L 0.0000 Buried
20 T L -0.6226 Soluble
21 I L 0.0000 Buried
22 T L -0.7844 Soluble
23 C L 0.0000 Buried
24 R L -2.8631 Soluble
25 A L 0.0000 Buried
26 S L -2.2153 Soluble
27 Q L -2.7160 Soluble
28 D L -2.7382 Soluble
29 V L 0.0000 Buried
36 N L -1.8578 Soluble
37 T L -0.8248 Soluble
38 A L 0.0610 Aggregation-prone
39 V L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.6447 Soluble
46 P L -1.3114 Soluble
47 G L -1.5467 Soluble
48 K L -2.4505 Soluble
49 A L -1.5315 Soluble
50 P L 0.0000 Buried
51 K L -1.3689 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.0000 Buried
56 S L 0.5319 Aggregation-prone
57 A L 0.0000 Buried
65 S L 0.3849 Aggregation-prone
66 F L 1.6753 Aggregation-prone
67 L L 1.2396 Aggregation-prone
68 Y L 0.5626 Aggregation-prone
69 S L -0.0032 Soluble
70 G L -0.3805 Soluble
71 V L -0.1805 Soluble
72 P L -0.2628 Soluble
74 S L -0.3346 Soluble
75 R L -0.7153 Soluble
76 F L 0.0000 Buried
77 S L 0.0554 Aggregation-prone
78 G L -0.0583 Soluble
79 S L -0.6640 Soluble
80 R L -1.6755 Soluble
83 S L -1.3981 Soluble
84 G L -1.8753 Soluble
85 T L -2.3045 Soluble
86 D L -2.1400 Soluble
87 F L 0.0000 Buried
88 T L -0.8031 Soluble
89 L L 0.0000 Buried
90 T L -0.6298 Soluble
91 I L 0.0000 Buried
92 S L -1.3682 Soluble
93 S L -1.1546 Soluble
94 L L 0.0000 Buried
95 Q L -0.8294 Soluble
96 P L -1.3693 Soluble
97 E L -1.9096 Soluble
98 D L 0.0000 Buried
99 F L -1.0903 Soluble
100 A L 0.0000 Buried
101 T L -0.7740 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 H L 0.4752 Aggregation-prone
108 Y L 0.5745 Aggregation-prone
109 T L 0.2142 Aggregation-prone
114 T L -0.4124 Soluble
115 P L -0.8260 Soluble
116 P L 0.0000 Buried
117 T L -0.6540 Soluble
118 F L -0.5190 Soluble
119 G L 0.0000 Buried
120 Q L -1.8362 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -1.4156 Soluble
124 V L 0.0000 Buried
125 E L -1.0058 Soluble
126 I L 0.0000 Buried
127 K L -0.9744 Soluble
128 R L -0.7069 Soluble
129 T L -0.0642 Soluble
130 V L 0.3235 Aggregation-prone
131 A L -0.0597 Soluble
132 A L -0.1551 Soluble
133 P L 0.0000 Buried
134 S L -0.1735 Soluble
135 V L 0.0000 Buried
136 F L 0.0395 Aggregation-prone
137 I L 0.0476 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.5189 Soluble
140 P L -0.8021 Soluble
141 S L -1.5890 Soluble
142 D L -2.8702 Soluble
143 E L -2.8247 Soluble
144 Q L 0.0000 Buried
145 L L -2.2318 Soluble
146 K L -2.8023 Soluble
147 S L -1.8013 Soluble
148 G L -1.3415 Soluble
149 T L -0.8932 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.9269 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.5201 Soluble
162 R L -2.6404 Soluble
163 E L -3.0273 Soluble
164 A L -2.2345 Soluble
165 K L -2.3787 Soluble
166 V L -1.0008 Soluble
167 Q L -0.5232 Soluble
168 W L 0.0000 Buried
169 K L -0.4935 Soluble
170 V L 0.0000 Buried
171 D L -2.0693 Soluble
172 N L -1.5732 Soluble
173 A L -0.2540 Soluble
174 L L 0.7958 Aggregation-prone
175 Q L -0.0402 Soluble
176 S L -0.3684 Soluble
177 G L -0.8390 Soluble
178 N L -0.7158 Soluble
179 S L -0.9452 Soluble
180 Q L -1.1267 Soluble
181 E L -1.5011 Soluble
182 S L -0.8044 Soluble
183 V L -0.7088 Soluble
184 T L -1.1272 Soluble
185 E L -2.1503 Soluble
186 Q L 0.0000 Buried
187 D L -2.1078 Soluble
188 S L -2.0850 Soluble
189 K L -2.4703 Soluble
190 D L -1.7869 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.5374 Soluble
199 L L 0.0000 Buried
200 T L -0.2802 Soluble
201 L L -0.3065 Soluble
202 S L -0.6524 Soluble
203 K L -1.6297 Soluble
204 A L -1.2123 Soluble
205 A L -1.2717 Soluble
206 Y L 0.0000 Buried
207 E L -2.9244 Soluble
208 K L -3.1502 Soluble
209 H L -2.7943 Soluble
210 K L -3.3308 Soluble
211 V L -1.5229 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
215 E L -0.8235 Soluble
216 V L 0.0000 Buried
217 T L -1.1598 Soluble
218 H L 0.0000 Buried
219 Q L -1.6777 Soluble
220 G L -0.4354 Soluble
221 L L -0.1798 Soluble
222 S L -0.4118 Soluble
223 S L -0.3794 Soluble
224 P L -0.4656 Soluble
225 V L 0.0405 Aggregation-prone
226 T L -0.4917 Soluble
227 K L -0.8405 Soluble
228 S L -0.5771 Soluble
229 F L -0.9260 Soluble
230 N L -1.9633 Soluble
231 R L -2.5432 Soluble
232 G L -2.1716 Soluble
233 E L -2.5105 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6984 2.8904 CSV PDB
4.5 -0.7418 2.8034 CSV PDB
5.0 -0.792 2.7013 CSV PDB
5.5 -0.8393 2.7013 CSV PDB
6.0 -0.8727 2.7013 CSV PDB
6.5 -0.8834 2.7013 CSV PDB
7.0 -0.8719 2.7013 CSV PDB
7.5 -0.8455 2.7012 CSV PDB
8.0 -0.8109 2.7012 CSV PDB
8.5 -0.7697 2.7012 CSV PDB
9.0 -0.7216 2.701 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH