Vixticibart / 8tg9

A4DAB296_8tg9_E_F | Source PDB: 8tg9 | Chains: E, F | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.646
Max Score
1.4875
Min Score
-3.9454
Total Score
-275.86
Score CDR
-0.0888
Score non-CDR
-0.7199

Calculation Parameters

Chain Sequence(s) VQLVQSGAEVKKPGASVTVSCKASGYTFTDYYMHWVRQAPGQGLEWMGWIKPNSGGTNSAQRFQGRITMTWDTSISTAYMELSRLRSDDTAVYYCSRGGPVMNYYYYYGMDVWGQGTTVTVSSASTKGPSVFPLAPCTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVNIQMTQSPSSLSASVGDRVTITCRASQSIDSYLNWYQQKPGKAPKLLIYVASSLQSGVPSRFSGSGSGKDFTLTISSLQPEDFATYYCQQSYSIPTFGQGTRLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) E (Heavy), F (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.9454
Maximum Score 1.4875
Average Score -0.646
Total Score -275.8565

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V E 1.0503 Aggregation-prone
3 Q E -0.3556 Soluble
4 L E 0.0000 Buried
5 V E -0.1717 Soluble
6 Q E 0.0000 Buried
7 S E -0.7764 Soluble
8 G E -1.0073 Soluble
9 A E -1.0099 Soluble
11 E E -1.7396 Soluble
12 V E -0.8070 Soluble
13 K E -1.3021 Soluble
14 K E -1.9527 Soluble
15 P E -1.9599 Soluble
16 G E -1.9410 Soluble
17 A E -1.4382 Soluble
18 S E -1.2140 Soluble
19 V E 0.0000 Buried
20 T E -0.7242 Soluble
21 V E 0.0000 Buried
22 S E -0.3191 Soluble
23 C E 0.0000 Buried
24 K E -0.8081 Soluble
25 A E 0.0000 Buried
26 S E -0.0761 Soluble
27 G E 0.0116 Aggregation-prone
28 Y E 0.1855 Aggregation-prone
29 T E -0.0010 Soluble
30 F E 0.0000 Buried
35 T E -0.7231 Soluble
36 D E -0.4841 Soluble
37 Y E 0.2271 Aggregation-prone
38 Y E 0.0000 Buried
39 M E 0.0000 Buried
40 H E 0.0000 Buried
41 W E 0.0000 Buried
42 V E 0.0000 Buried
43 R E 0.0000 Buried
44 Q E -0.5596 Soluble
45 A E 0.0000 Buried
46 P E -0.8049 Soluble
47 G E -1.2277 Soluble
48 Q E -1.8336 Soluble
49 G E -1.2705 Soluble
50 L E 0.0000 Buried
51 E E -0.6548 Soluble
52 W E 0.0000 Buried
53 M E 0.0000 Buried
54 G E 0.0000 Buried
55 W E 0.0000 Buried
56 I E 0.0000 Buried
57 K E -0.7189 Soluble
58 P E 0.0000 Buried
59 N E -1.5516 Soluble
62 S E -1.1710 Soluble
63 G E -1.0838 Soluble
64 G E -0.8889 Soluble
65 T E -0.6220 Soluble
66 N E -0.9337 Soluble
67 S E -1.2313 Soluble
68 A E 0.0000 Buried
69 Q E -2.5853 Soluble
70 R E -2.9128 Soluble
71 F E 0.0000 Buried
72 Q E -2.5310 Soluble
74 G E -1.6134 Soluble
75 R E -1.6019 Soluble
76 I E -0.9365 Soluble
77 T E -0.5902 Soluble
78 M E -0.1333 Soluble
79 T E -0.1495 Soluble
80 W E -0.0189 Soluble
81 D E -0.2384 Soluble
82 T E -0.0636 Soluble
83 S E 0.4031 Aggregation-prone
84 I E 1.2270 Aggregation-prone
85 S E 0.2436 Aggregation-prone
86 T E 0.0000 Buried
87 A E 0.0000 Buried
88 Y E -0.1279 Soluble
89 M E 0.0000 Buried
90 E E -1.1057 Soluble
91 L E 0.0000 Buried
92 S E -1.6892 Soluble
93 R E -2.6196 Soluble
94 L E 0.0000 Buried
95 R E -2.8873 Soluble
96 S E -1.8460 Soluble
97 D E -1.8233 Soluble
98 D E 0.0000 Buried
99 T E -0.6989 Soluble
100 A E 0.0000 Buried
101 V E 0.0119 Aggregation-prone
102 Y E 0.0000 Buried
103 Y E 0.0000 Buried
104 C E 0.0000 Buried
105 S E 0.0000 Buried
106 R E -0.0591 Soluble
107 G E 0.0000 Buried
108 G E 0.0000 Buried
109 P E 0.5896 Aggregation-prone
110 V E 0.0000 Buried
111 M E 0.6837 Aggregation-prone
111A N E 0.0626 Aggregation-prone
111B Y E 1.4692 Aggregation-prone
112B Y E 1.3606 Aggregation-prone
112A Y E 1.2946 Aggregation-prone
112 Y E 1.4875 Aggregation-prone
113 Y E 1.0727 Aggregation-prone
114 G E 0.1902 Aggregation-prone
115 M E 0.0000 Buried
116 D E -1.0451 Soluble
117 V E -0.2944 Soluble
118 W E -0.7464 Soluble
119 G E 0.0000 Buried
120 Q E -1.3700 Soluble
121 G E -0.8283 Soluble
122 T E 0.0000 Buried
123 T E -0.4420 Soluble
124 V E 0.0000 Buried
125 T E -0.8821 Soluble
126 V E 0.0000 Buried
127 S E -1.0445 Soluble
128 S E -0.6924 Soluble
129 A E -0.5979 Soluble
130 S E -0.6616 Soluble
131 T E -1.0602 Soluble
132 K E -1.8428 Soluble
133 G E -1.9854 Soluble
134 P E -1.0686 Soluble
135 S E -0.4604 Soluble
136 V E 0.0000 Buried
137 F E -0.3235 Soluble
138 P E -0.2933 Soluble
139 L E 0.0000 Buried
140 A E 0.3730 Aggregation-prone
141 P E 0.3931 Aggregation-prone
142 C E 0.5822 Aggregation-prone
143 T E -0.1141 Soluble
144 A E 0.0954 Aggregation-prone
145 A E 0.0000 Buried
146 L E 0.0000 Buried
147 G E 0.0000 Buried
148 C E 0.0000 Buried
149 L E 0.1921 Aggregation-prone
150 V E 0.0000 Buried
151 K E -0.5561 Soluble
152 D E -1.2274 Soluble
153 Y E 0.0000 Buried
154 F E 0.0000 Buried
155 P E -0.9730 Soluble
156 E E -1.1394 Soluble
157 P E -1.0685 Soluble
158 V E -0.7008 Soluble
159 T E -0.5630 Soluble
160 V E -0.1120 Soluble
161 S E -0.5234 Soluble
162 W E 0.0000 Buried
163 N E -0.9881 Soluble
164 S E -0.7814 Soluble
165 G E -0.5911 Soluble
166 A E -0.2617 Soluble
167 L E -0.0387 Soluble
168 T E -0.1852 Soluble
169 S E -0.1796 Soluble
170 G E -0.1650 Soluble
171 V E 0.1246 Aggregation-prone
172 H E -0.2048 Soluble
173 T E -0.0394 Soluble
174 F E 0.0000 Buried
175 P E -0.2328 Soluble
176 A E 0.3894 Aggregation-prone
177 V E 0.6967 Aggregation-prone
178 L E 1.3777 Aggregation-prone
179 Q E 0.1590 Aggregation-prone
180 S E -0.0685 Soluble
181 S E -0.3129 Soluble
182 G E -0.1650 Soluble
183 L E 0.0000 Buried
184 Y E 0.2520 Aggregation-prone
185 S E 0.0000 Buried
186 L E 0.3203 Aggregation-prone
187 S E 0.0678 Aggregation-prone
188 S E 0.0000 Buried
189 V E 0.0000 Buried
190 V E 0.0000 Buried
191 T E -0.1596 Soluble
192 V E 0.0000 Buried
193 P E -0.1417 Soluble
194 S E -0.0183 Soluble
195 S E -0.1842 Soluble
196 S E 0.0000 Buried
197 L E 0.7604 Aggregation-prone
198 G E -0.3610 Soluble
199 T E -0.7556 Soluble
200 K E -1.6139 Soluble
201 T E -0.9622 Soluble
202 Y E 0.0000 Buried
203 T E -1.2624 Soluble
204 C E 0.0000 Buried
205 N E -1.7346 Soluble
206 V E 0.0000 Buried
207 D E -2.2144 Soluble
208 H E 0.0000 Buried
209 K E -2.8678 Soluble
210 P E -1.8220 Soluble
211 S E -2.0116 Soluble
212 N E -2.7104 Soluble
213 T E -2.3919 Soluble
214 K E -2.7184 Soluble
215 V E -1.8043 Soluble
216 D E -2.7090 Soluble
217 K E -2.0255 Soluble
218 R E -1.9887 Soluble
219 V E -0.1500 Soluble
1 N F -1.5519 Soluble
2 I F -1.2051 Soluble
3 Q F -1.9114 Soluble
4 M F 0.0000 Buried
5 T F -1.2813 Soluble
6 Q F 0.0000 Buried
7 S F -0.6837 Soluble
8 P F -0.5300 Soluble
9 S F -0.9160 Soluble
10 S F -0.9907 Soluble
11 L F -0.6786 Soluble
12 S F -1.0038 Soluble
13 A F 0.0000 Buried
14 S F -0.5220 Soluble
15 V F 0.5727 Aggregation-prone
16 G F -0.6670 Soluble
17 D F -1.5371 Soluble
18 R F -2.2268 Soluble
19 V F 0.0000 Buried
20 T F -0.4866 Soluble
21 I F 0.0000 Buried
22 T F -0.9431 Soluble
23 C F 0.0000 Buried
24 R F -3.3922 Soluble
25 A F -2.7055 Soluble
26 S F -2.2529 Soluble
27 Q F -2.3468 Soluble
28 S F -2.1212 Soluble
29 I F 0.0000 Buried
36 D F -1.9825 Soluble
37 S F -0.7774 Soluble
38 Y F 0.3661 Aggregation-prone
39 L F 0.0000 Buried
40 N F 0.0000 Buried
41 W F 0.0000 Buried
42 Y F 0.0000 Buried
43 Q F 0.0000 Buried
44 Q F 0.0000 Buried
45 K F -1.5958 Soluble
46 P F -1.4502 Soluble
47 G F -1.6700 Soluble
48 K F -2.5285 Soluble
49 A F -1.5899 Soluble
50 P F 0.0000 Buried
51 K F -1.7614 Soluble
52 L F 0.0000 Buried
53 L F 0.0000 Buried
54 I F 0.0000 Buried
55 Y F 0.6907 Aggregation-prone
56 V F 0.6135 Aggregation-prone
57 A F 0.0000 Buried
65 S F -0.0608 Soluble
66 S F 0.2011 Aggregation-prone
67 L F 0.3642 Aggregation-prone
68 Q F -0.2551 Soluble
69 S F -0.4095 Soluble
70 G F -0.5148 Soluble
71 V F -0.3595 Soluble
72 P F -0.3585 Soluble
74 S F -0.4695 Soluble
75 R F -0.8327 Soluble
76 F F 0.0000 Buried
77 S F -0.3804 Soluble
78 G F -0.2362 Soluble
79 S F -0.7242 Soluble
80 G F -1.5429 Soluble
83 S F -1.9372 Soluble
84 G F -2.7064 Soluble
85 K F -3.9454 Soluble
86 D F -3.4644 Soluble
87 F F 0.0000 Buried
88 T F -0.8862 Soluble
89 L F 0.0000 Buried
90 T F -0.5989 Soluble
91 I F 0.0000 Buried
92 S F -1.2578 Soluble
93 S F -1.0108 Soluble
94 L F 0.0000 Buried
95 Q F -0.9592 Soluble
96 P F -1.0336 Soluble
97 E F -2.0821 Soluble
98 D F 0.0000 Buried
99 F F -0.7571 Soluble
100 A F 0.0000 Buried
101 T F 0.0000 Buried
102 Y F 0.0000 Buried
103 Y F 0.0000 Buried
104 C F 0.0000 Buried
105 Q F 0.0000 Buried
106 Q F 0.0000 Buried
107 S F 0.0000 Buried
108 Y F 0.9728 Aggregation-prone
114 S F 0.5682 Aggregation-prone
115 I F 0.2601 Aggregation-prone
116 P F 0.3538 Aggregation-prone
117 T F -0.2786 Soluble
118 F F -0.3900 Soluble
119 G F -1.0164 Soluble
120 Q F -1.5328 Soluble
121 G F -1.3065 Soluble
122 T F 0.0000 Buried
123 R F -2.0095 Soluble
124 L F 0.0000 Buried
125 E F -1.0544 Soluble
126 I F -0.5560 Soluble
127 K F -1.5261 Soluble
128 R F -1.1740 Soluble
129 T F -0.0327 Soluble
130 V F 0.6791 Aggregation-prone
131 A F 0.2452 Aggregation-prone
132 A F -0.0607 Soluble
133 P F -0.0724 Soluble
134 S F -0.1645 Soluble
135 V F 0.0000 Buried
136 F F 0.2734 Aggregation-prone
137 I F 0.3311 Aggregation-prone
138 F F 0.0000 Buried
139 P F -0.0283 Soluble
140 P F -0.4525 Soluble
141 S F -1.2945 Soluble
142 D F -2.5801 Soluble
143 E F -2.4678 Soluble
144 Q F 0.0000 Buried
145 L F -2.0455 Soluble
146 K F -2.6894 Soluble
147 S F -1.6759 Soluble
148 G F -1.2197 Soluble
149 T F -0.9560 Soluble
150 A F 0.0000 Buried
151 S F 0.0000 Buried
152 V F 0.0000 Buried
153 V F 0.0000 Buried
154 C F 0.0000 Buried
155 L F 0.0000 Buried
156 L F 0.0000 Buried
157 N F 0.0000 Buried
158 N F -1.1679 Soluble
159 F F 0.0000 Buried
160 Y F 0.0000 Buried
161 P F -1.8137 Soluble
162 R F -3.1760 Soluble
163 E F -3.2660 Soluble
164 A F -2.4007 Soluble
165 K F -2.3953 Soluble
166 V F -1.0421 Soluble
167 Q F -0.4795 Soluble
168 W F 0.0000 Buried
169 K F -0.6866 Soluble
170 V F 0.0000 Buried
171 D F -1.7574 Soluble
172 N F -1.4077 Soluble
173 A F -0.3332 Soluble
174 L F 0.7981 Aggregation-prone
175 Q F -0.3354 Soluble
176 S F -0.7173 Soluble
177 G F -1.2776 Soluble
178 N F -1.5702 Soluble
179 S F -1.5851 Soluble
180 Q F -1.8746 Soluble
181 E F -1.8382 Soluble
182 S F -0.7202 Soluble
183 V F -0.7209 Soluble
184 T F -1.2476 Soluble
185 E F -2.4654 Soluble
186 Q F -2.4536 Soluble
187 D F -2.8264 Soluble
188 S F -2.2917 Soluble
189 K F -2.8401 Soluble
190 D F -2.8121 Soluble
191 S F -2.5340 Soluble
192 T F 0.0000 Buried
193 Y F 0.0000 Buried
194 S F 0.0000 Buried
195 L F 0.0000 Buried
196 S F 0.0000 Buried
197 S F 0.0000 Buried
198 T F -0.8884 Soluble
199 L F 0.0000 Buried
200 T F -0.4823 Soluble
201 L F -0.5317 Soluble
202 S F -0.8861 Soluble
203 K F -1.7304 Soluble
204 A F -1.4366 Soluble
205 D F -2.0252 Soluble
206 Y F 0.0000 Buried
207 E F -2.6295 Soluble
208 K F -2.9803 Soluble
209 H F -2.4007 Soluble
210 K F -2.4892 Soluble
211 V F -1.4658 Soluble
212 Y F 0.0000 Buried
213 A F 0.0000 Buried
214 C F 0.0000 Buried
215 E F -0.7208 Soluble
216 V F 0.0000 Buried
217 T F -1.1717 Soluble
218 H F 0.0000 Buried
219 Q F -1.5935 Soluble
220 G F -0.3989 Soluble
221 L F -0.2314 Soluble
222 S F -0.4923 Soluble
223 S F -0.4664 Soluble
224 P F -0.5576 Soluble
225 V F -0.0575 Soluble
226 T F -0.4548 Soluble
227 K F -0.5557 Soluble
228 S F -0.5202 Soluble
229 F F 0.0000 Buried
230 N F -1.3864 Soluble
231 R F -1.9191 Soluble
232 G F -1.8141 Soluble
233 E F -1.9315 Soluble
234 C F -0.5361 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6457 2.8625 CSV PDB
4.5 -0.6866 2.8262 CSV PDB
5.0 -0.7341 2.7706 CSV PDB
5.5 -0.7791 2.7046 CSV PDB
6.0 -0.8117 2.6409 CSV PDB
6.5 -0.8248 2.6409 CSV PDB
7.0 -0.8187 2.6409 CSV PDB
7.5 -0.7999 2.6409 CSV PDB
8.0 -0.774 2.6409 CSV PDB
8.5 -0.7425 2.6409 CSV PDB
9.0 -0.7045 2.6409 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH