Vilastobart / 8g8n

Calculation Parameters

Chain Sequence(s)	`QVQLVQSGAEVKKPGSSVKVSCKASGYTFTNYFMNWVRQAPGQGLEWMGRVDPEQGRADYAEKFKKRVTITADKSTSTAYMELSSLRSEDTAVYYCARRAMDNYGFAYWGQGTLVTVSSASTKGPSVFPLAPSSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCEIVLTQSPDFQSVTPKEKVTITCSANSAYMYWYQQKPDQSPKLWVHGTSNLASGVPSRFSGSGSGTDFTLTINSLEAEDAATYYCHHWSNTQWTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC` input.pdb
Selected Chain(s)	D (Heavy), E (Light)
Distance of Aggregation	10 Å
FoldX Energy Minimization	Yes
pH Calculations	Yes
Downloads	Download all data (.tar.gz)

Aggrescan4D Profile

Chain: Show buried residues (score = 0)

Aggregation-prone (score > 0)

Soluble (score < 0)

Buried (score = 0)

Per-Residue A4D Scores

Download CSV

Minimum Score -3.6535

Maximum Score 1.4087

Average Score -0.7743

Total Score -331.3894

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Classification:

Index	Residue	Chain	A4D Score	Classification
1	Q	D	-1.6819	Soluble
2	V	D	0.0000	Buried
3	Q	D	-1.4709	Soluble
4	L	D	0.0000	Buried
5	V	D	0.5382	Aggregation-prone
6	Q	D	0.0000	Buried
7	S	D	-0.5396	Soluble
8	G	D	-0.6331	Soluble
9	A	D	-0.5009	Soluble
11	E	D	-1.0345	Soluble
12	V	D	-0.4746	Soluble
13	K	D	-1.3806	Soluble
14	K	D	-1.9057	Soluble
15	P	D	-2.0314	Soluble
16	G	D	-1.4687	Soluble
17	S	D	-1.1702	Soluble
18	S	D	-1.4430	Soluble
19	V	D	0.0000	Buried
20	K	D	-2.1888	Soluble
21	V	D	0.0000	Buried
22	S	D	-0.5132	Soluble
23	C	D	0.0000	Buried
24	K	D	-0.5691	Soluble
25	A	D	0.0000	Buried
26	S	D	-0.9323	Soluble
27	G	D	-1.2647	Soluble
28	Y	D	-0.7280	Soluble
29	T	D	-0.6404	Soluble
30	F	D	0.0000	Buried
35	T	D	-1.9755	Soluble
36	N	D	-1.5508	Soluble
37	Y	D	-0.5858	Soluble
38	F	D	-0.4687	Soluble
39	M	D	0.0000	Buried
40	N	D	0.0000	Buried
41	W	D	0.0000	Buried
42	V	D	0.0000	Buried
43	R	D	-0.5366	Soluble
44	Q	D	0.0000	Buried
45	A	D	-1.0287	Soluble
46	P	D	-1.0015	Soluble
47	G	D	-1.2235	Soluble
48	Q	D	-1.7611	Soluble
49	G	D	-1.2181	Soluble
50	L	D	0.0000	Buried
51	E	D	-1.3818	Soluble
52	W	D	0.0000	Buried
53	M	D	0.0000	Buried
54	G	D	0.0000	Buried
55	R	D	0.0000	Buried
56	V	D	0.0000	Buried
57	D	D	-1.9509	Soluble
58	P	D	0.0000	Buried
59	E	D	-3.5449	Soluble
62	Q	D	-3.1142	Soluble
63	G	D	-2.8154	Soluble
64	R	D	-2.7531	Soluble
65	A	D	-1.1165	Soluble
66	D	D	0.0000	Buried
67	Y	D	-1.3286	Soluble
68	A	D	0.0000	Buried
69	E	D	-3.6535	Soluble
70	K	D	-3.5198	Soluble
71	F	D	0.0000	Buried
72	K	D	-3.6480	Soluble
74	K	D	-3.3598	Soluble
75	R	D	-2.1795	Soluble
76	V	D	0.0000	Buried
77	T	D	-1.1226	Soluble
78	I	D	0.0000	Buried
79	T	D	-0.7173	Soluble
80	A	D	-1.6786	Soluble
81	D	D	-2.1590	Soluble
82	K	D	-2.8224	Soluble
83	S	D	-1.3584	Soluble
84	T	D	-1.1094	Soluble
85	S	D	-1.2543	Soluble
86	T	D	-0.8713	Soluble
87	A	D	0.0000	Buried
88	Y	D	-0.7014	Soluble
89	M	D	0.0000	Buried
90	E	D	-1.6610	Soluble
91	L	D	0.0000	Buried
92	S	D	-1.4080	Soluble
93	S	D	-1.2813	Soluble
94	L	D	0.0000	Buried
95	R	D	-2.8893	Soluble
96	S	D	-2.2443	Soluble
97	E	D	-2.4917	Soluble
98	D	D	0.0000	Buried
99	T	D	-0.9245	Soluble
100	A	D	0.0000	Buried
101	V	D	0.1957	Aggregation-prone
102	Y	D	0.0000	Buried
103	Y	D	0.0000	Buried
104	C	D	0.0000	Buried
105	A	D	0.0000	Buried
106	R	D	0.0000	Buried
107	R	D	0.0028	Aggregation-prone
108	A	D	0.0000	Buried
109	M	D	0.1055	Aggregation-prone
110	D	D	-1.2977	Soluble
112	N	D	-0.5761	Soluble
113	Y	D	0.7305	Aggregation-prone
114	G	D	0.0000	Buried
115	F	D	0.0000	Buried
116	A	D	-0.1650	Soluble
117	Y	D	-0.1792	Soluble
118	W	D	-0.3743	Soluble
119	G	D	0.0000	Buried
120	Q	D	-1.3423	Soluble
121	G	D	-0.7059	Soluble
122	T	D	0.0000	Buried
123	L	D	-0.1541	Soluble
124	V	D	0.0000	Buried
125	T	D	0.0000	Buried
126	V	D	0.0000	Buried
127	S	D	-1.2366	Soluble
128	S	D	-0.9409	Soluble
129	A	D	-0.5515	Soluble
130	S	D	-0.6324	Soluble
131	T	D	-0.6263	Soluble
132	K	D	-1.3317	Soluble
133	G	D	-1.3941	Soluble
134	P	D	0.0000	Buried
135	S	D	-0.1819	Soluble
136	V	D	0.0000	Buried
137	F	D	-0.5187	Soluble
138	P	D	-0.8709	Soluble
139	L	D	0.0000	Buried
140	A	D	-0.8123	Soluble
141	P	D	0.0000	Buried
142	S	D	-0.6271	Soluble
143	S	D	-0.2687	Soluble
144	G	D	-0.6253	Soluble
145	T	D	-0.4764	Soluble
146	A	D	-0.2427	Soluble
147	A	D	0.0000	Buried
148	L	D	0.0000	Buried
149	G	D	0.0000	Buried
150	C	D	0.0000	Buried
151	L	D	0.0000	Buried
152	V	D	0.0000	Buried
153	K	D	0.0000	Buried
154	D	D	-0.4494	Soluble
155	Y	D	0.0000	Buried
156	F	D	0.0000	Buried
157	P	D	0.0000	Buried
158	E	D	-0.2915	Soluble
159	P	D	-0.6008	Soluble
160	V	D	-0.5598	Soluble
161	T	D	-0.4791	Soluble
162	V	D	-0.1615	Soluble
163	S	D	-0.3849	Soluble
164	W	D	0.0000	Buried
165	N	D	-0.7380	Soluble
166	S	D	-0.6782	Soluble
167	G	D	-0.5225	Soluble
168	A	D	-0.2528	Soluble
169	L	D	0.0048	Aggregation-prone
170	T	D	-0.1907	Soluble
171	S	D	-0.2159	Soluble
172	G	D	-0.3353	Soluble
173	V	D	0.1002	Aggregation-prone
174	H	D	-0.3200	Soluble
175	T	D	-0.1345	Soluble
176	F	D	0.0000	Buried
177	P	D	-0.2175	Soluble
178	A	D	0.3140	Aggregation-prone
179	V	D	0.6227	Aggregation-prone
180	L	D	1.4087	Aggregation-prone
181	Q	D	0.4079	Aggregation-prone
182	S	D	-0.1419	Soluble
183	S	D	-0.1764	Soluble
184	G	D	0.0699	Aggregation-prone
185	L	D	0.1088	Aggregation-prone
186	Y	D	0.0000	Buried
187	S	D	0.0000	Buried
188	L	D	0.0000	Buried
189	S	D	0.0000	Buried
190	S	D	0.0000	Buried
191	V	D	0.0000	Buried
192	V	D	0.0000	Buried
193	T	D	-0.1584	Soluble
194	V	D	0.0000	Buried
195	P	D	-0.5231	Soluble
196	S	D	-0.4553	Soluble
197	S	D	-0.4883	Soluble
198	S	D	-0.4683	Soluble
199	L	D	-0.5865	Soluble
200	G	D	-1.1700	Soluble
201	T	D	-0.8913	Soluble
202	Q	D	-1.2858	Soluble
203	T	D	-1.0728	Soluble
204	Y	D	0.0000	Buried
205	I	D	-1.0052	Soluble
206	C	D	0.0000	Buried
207	N	D	-1.4230	Soluble
208	V	D	0.0000	Buried
209	N	D	-1.8578	Soluble
210	H	D	0.0000	Buried
211	K	D	-2.7556	Soluble
212	P	D	-1.7405	Soluble
213	S	D	-1.9250	Soluble
214	N	D	-2.6190	Soluble
215	T	D	-2.0707	Soluble
216	K	D	-2.6449	Soluble
217	V	D	-1.5191	Soluble
218	D	D	-2.4102	Soluble
219	K	D	-1.7813	Soluble
220	K	D	-2.0907	Soluble
221	V	D	0.0000	Buried
222	E	D	-2.5085	Soluble
223	P	D	-1.2508	Soluble
224	K	D	-1.2017	Soluble
225	S	D	-0.5139	Soluble
226	C	D	0.2589	Aggregation-prone
1	E	E	-1.8187	Soluble
2	I	E	-0.2767	Soluble
3	V	E	0.9134	Aggregation-prone
4	L	E	0.0000	Buried
5	T	E	-0.3701	Soluble
6	Q	E	0.0000	Buried
7	S	E	-0.8712	Soluble
8	P	E	-1.0647	Soluble
9	D	E	-1.8554	Soluble
10	F	E	-0.6056	Soluble
11	Q	E	-0.8490	Soluble
12	S	E	-0.7253	Soluble
13	V	E	0.0000	Buried
14	T	E	-1.7783	Soluble
15	P	E	-2.4901	Soluble
16	K	E	-3.2114	Soluble
17	E	E	-3.2688	Soluble
18	K	E	-3.1946	Soluble
19	V	E	0.0000	Buried
20	T	E	-0.6979	Soluble
21	I	E	0.0000	Buried
22	T	E	-0.7461	Soluble
23	C	E	0.0000	Buried
24	S	E	-0.9431	Soluble
25	A	E	-0.6906	Soluble
26	N	E	-1.4818	Soluble
27	S	E	-1.0912	Soluble
28	A	E	-0.4506	Soluble
38	Y	E	1.2417	Aggregation-prone
39	M	E	0.0000	Buried
40	Y	E	0.0000	Buried
41	W	E	0.0000	Buried
42	Y	E	0.0000	Buried
43	Q	E	0.0000	Buried
44	Q	E	0.0000	Buried
45	K	E	-1.7640	Soluble
46	P	E	-2.0714	Soluble
47	D	E	-2.7679	Soluble
48	Q	E	-2.6426	Soluble
49	S	E	-1.6053	Soluble
50	P	E	0.0000	Buried
51	K	E	-1.2272	Soluble
52	L	E	0.0000	Buried
53	W	E	0.0000	Buried
54	V	E	0.0000	Buried
55	H	E	-0.3794	Soluble
56	G	E	-0.1297	Soluble
57	T	E	-0.2651	Soluble
65	S	E	-0.7827	Soluble
66	N	E	-1.3087	Soluble
67	L	E	-0.4329	Soluble
68	A	E	-0.4598	Soluble
69	S	E	-0.4239	Soluble
70	G	E	-0.4600	Soluble
71	V	E	-0.4187	Soluble
72	P	E	-0.3558	Soluble
74	S	E	-0.6359	Soluble
75	R	E	-1.4157	Soluble
76	F	E	0.0000	Buried
77	S	E	-0.7727	Soluble
78	G	E	-0.5991	Soluble
79	S	E	-0.7318	Soluble
80	G	E	-0.8760	Soluble
83	S	E	-0.9442	Soluble
84	G	E	-1.3511	Soluble
85	T	E	-1.6244	Soluble
86	D	E	-2.2779	Soluble
87	F	E	0.0000	Buried
88	T	E	-0.6574	Soluble
89	L	E	0.0000	Buried
90	T	E	-0.8798	Soluble
91	I	E	0.0000	Buried
92	N	E	-2.9417	Soluble
93	S	E	-2.9088	Soluble
94	L	E	0.0000	Buried
95	E	E	-2.4471	Soluble
96	A	E	-1.9920	Soluble
97	E	E	-2.2339	Soluble
98	D	E	0.0000	Buried
99	A	E	-1.1604	Soluble
100	A	E	0.0000	Buried
101	T	E	-0.7211	Soluble
102	Y	E	0.0000	Buried
103	Y	E	0.0000	Buried
104	C	E	0.0000	Buried
105	H	E	0.0000	Buried
106	H	E	0.0000	Buried
107	W	E	0.1078	Aggregation-prone
108	S	E	-0.7223	Soluble
109	N	E	-1.5416	Soluble
114	T	E	-1.0894	Soluble
115	Q	E	-1.0537	Soluble
116	W	E	0.0000	Buried
117	T	E	-0.0953	Soluble
118	F	E	0.0000	Buried
119	G	E	0.0000	Buried
120	G	E	-1.0997	Soluble
121	G	E	0.0000	Buried
122	T	E	0.0000	Buried
123	K	E	-1.2450	Soluble
124	V	E	0.0000	Buried
125	E	E	-1.1404	Soluble
126	I	E	0.0000	Buried
127	K	E	-1.1996	Soluble
128	R	E	-0.7458	Soluble
129	T	E	0.2916	Aggregation-prone
130	V	E	1.0974	Aggregation-prone
131	A	E	0.4402	Aggregation-prone
132	A	E	0.0067	Aggregation-prone
133	P	E	0.0000	Buried
134	S	E	-0.1478	Soluble
135	V	E	0.0000	Buried
136	F	E	0.0823	Aggregation-prone
137	I	E	0.1103	Aggregation-prone
138	F	E	0.0000	Buried
139	P	E	-0.6094	Soluble
140	P	E	0.0000	Buried
141	S	E	-1.5413	Soluble
142	D	E	-2.7881	Soluble
143	E	E	-2.6486	Soluble
144	Q	E	0.0000	Buried
145	L	E	-2.2520	Soluble
146	K	E	-2.7820	Soluble
147	S	E	-1.7756	Soluble
148	G	E	-1.2983	Soluble
149	T	E	-1.0916	Soluble
150	A	E	0.0000	Buried
151	S	E	0.0000	Buried
152	V	E	0.0000	Buried
153	V	E	0.0000	Buried
154	C	E	0.0000	Buried
155	L	E	0.0000	Buried
156	L	E	0.0000	Buried
157	N	E	0.0000	Buried
158	N	E	-0.8398	Soluble
159	F	E	0.0000	Buried
160	Y	E	0.0000	Buried
161	P	E	-1.2695	Soluble
162	R	E	-2.1776	Soluble
163	E	E	-2.9451	Soluble
164	A	E	-2.1867	Soluble
165	K	E	-2.4077	Soluble
166	V	E	-1.3452	Soluble
167	Q	E	-1.3002	Soluble
168	W	E	0.0000	Buried
169	K	E	-1.0847	Soluble
170	V	E	0.0000	Buried
171	D	E	-1.8481	Soluble
172	N	E	-1.5982	Soluble
173	A	E	-0.4462	Soluble
174	L	E	0.2850	Aggregation-prone
175	Q	E	-0.6607	Soluble
176	S	E	-0.8520	Soluble
177	G	E	-1.3277	Soluble
178	N	E	-1.7144	Soluble
179	S	E	-1.4511	Soluble
180	Q	E	-1.1744	Soluble
181	E	E	-1.4631	Soluble
182	S	E	0.0000	Buried
183	V	E	-0.4644	Soluble
184	T	E	0.0000	Buried
185	E	E	-2.1598	Soluble
186	Q	E	-1.8333	Soluble
187	D	E	-1.9290	Soluble
188	S	E	-1.9817	Soluble
189	K	E	-2.1904	Soluble
190	D	E	-1.6513	Soluble
191	S	E	-1.6911	Soluble
192	T	E	0.0000	Buried
193	Y	E	0.0000	Buried
194	S	E	0.0000	Buried
195	L	E	0.0000	Buried
196	S	E	0.0000	Buried
197	S	E	0.0000	Buried
198	T	E	-0.6345	Soluble
199	L	E	0.0000	Buried
200	T	E	-0.6700	Soluble
201	L	E	-0.9112	Soluble
202	S	E	-1.2234	Soluble
203	K	E	-2.2246	Soluble
204	A	E	-2.0213	Soluble
205	D	E	-2.9068	Soluble
206	Y	E	0.0000	Buried
207	E	E	-3.1713	Soluble
208	K	E	-3.5117	Soluble
209	H	E	-2.8119	Soluble
210	K	E	-2.6052	Soluble
211	V	E	-1.1938	Soluble
212	Y	E	0.0000	Buried
213	A	E	-1.0175	Soluble
214	C	E	0.0000	Buried
215	E	E	-1.3383	Soluble
216	V	E	0.0000	Buried
217	T	E	-1.3276	Soluble
218	H	E	0.0000	Buried
219	Q	E	-1.6000	Soluble
220	G	E	-0.2853	Soluble
221	L	E	-0.1721	Soluble
222	S	E	-0.4654	Soluble
223	S	E	-0.4001	Soluble
224	P	E	-0.5396	Soluble
225	V	E	0.0020	Aggregation-prone
226	T	E	-0.6436	Soluble
227	K	E	-0.7544	Soluble
228	S	E	-0.6063	Soluble
229	F	E	-0.8699	Soluble
230	N	E	-1.7345	Soluble
231	R	E	-2.2490	Soluble
232	G	E	-1.7359	Soluble
233	E	E	-2.0892	Soluble
234	C	E	-0.7306	Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer | colored by A4D score

Load structure to view sequence…

Downloads

Download PDB View in RCSB

Color Legend (B-factor = A4D Score)

Score > 1.5 — highly aggregation-prone

Score 0.5–1.5 — aggregation-prone

Score ≈ 0 — buried

Score −1 to −0.5 — soluble

Score < −2.5 — highly soluble

pH Structure

Load structure at specific pH condition

A4D Score Threshold

Highlight residues > threshold in the sequence panel

Threshold: 0.0

CDR Regions

Outline CDR regions in sequence panel

HCDR1

HCDR2

HCDR3

LCDR1

LCDR2

LCDR3

Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH	Avg A4D Score	Max A4D Score	Downloads
4.0	-0.7658	1.8276	CSV PDB
4.5	-0.8172	1.8034	CSV PDB
5.0	-0.8785	1.7665	CSV PDB
5.5	-0.9378	1.7219	CSV PDB
6.0	-0.9809	1.6741	CSV PDB
6.5	-0.9959	1.6365	CSV PDB
7.0	-0.9812	1.7006	CSV PDB
7.5	-0.946	1.7751	CSV PDB
8.0	-0.8998	1.8517	CSV PDB
8.5	-0.8458	1.928	CSV PDB
9.0	-0.7848	2.0015	CSV PDB