Empasiprubart / 8acf

A4DAB103_8acf_H_L | Source PDB: 8acf | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7348
Max Score
1.1649
Min Score
-3.6993
Total Score
-318.92
Score CDR
-0.6285
Score non-CDR
-0.7484

Calculation Parameters

Chain Sequence(s) EVQLVQSGAEVKKPGASVKVSCKASGYTFTDYNMDWVRQATGQGLEWIGDINPNYESTGYNQKFKGRATMTVDKSISTAYMELSSLRSEDTAVYYCAREDDHDAFAYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKDNVLTQSPDSLAVSLGERATISCRASKSVRTSGYNYMHWYQQKPGQPPKLLIYLASNLKSGVPDRFSGSGSGTDFTLTISSLQAEDAATYYCQHSRELPYTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNR input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.6993
Maximum Score 1.1649
Average Score -0.7348
Total Score -318.9179

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.1612 Soluble
2 V H -1.3496 Soluble
3 Q H -1.6277 Soluble
4 L H 0.0000 Buried
5 V H 0.0922 Aggregation-prone
6 Q H 0.0000 Buried
7 S H -0.5665 Soluble
8 G H -0.5977 Soluble
9 A H -0.3290 Soluble
11 E H -0.6725 Soluble
12 V H -0.2313 Soluble
13 K H -1.2090 Soluble
14 K H -1.9085 Soluble
15 P H -2.0272 Soluble
16 G H -1.5173 Soluble
17 A H -1.0468 Soluble
18 S H -1.2213 Soluble
19 V H 0.0000 Buried
20 K H -1.7448 Soluble
21 V H 0.0000 Buried
22 S H -0.5271 Soluble
23 C H 0.0000 Buried
24 K H -0.7258 Soluble
25 A H 0.0000 Buried
26 S H -0.9146 Soluble
27 G H -1.2570 Soluble
28 Y H -0.7553 Soluble
29 T H -0.5847 Soluble
30 F H 0.0000 Buried
35 T H -1.3298 Soluble
36 D H -2.1485 Soluble
37 Y H -1.3445 Soluble
38 N H -1.1056 Soluble
39 M H 0.0000 Buried
40 D H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H -0.3845 Soluble
44 Q H -0.6135 Soluble
45 A H -0.8961 Soluble
46 T H -0.6801 Soluble
47 G H -1.1597 Soluble
48 Q H -1.7387 Soluble
49 G H -1.2246 Soluble
50 L H 0.0000 Buried
51 E H -0.7869 Soluble
52 W H 0.0000 Buried
53 I H 0.0000 Buried
54 G H 0.0000 Buried
55 D H -0.4367 Soluble
56 I H 0.0000 Buried
57 N H -1.0822 Soluble
58 P H 0.0000 Buried
59 N H -1.4835 Soluble
62 Y H -0.0272 Soluble
63 E H -1.4098 Soluble
64 S H -0.7361 Soluble
65 T H -0.3575 Soluble
66 G H 0.0672 Aggregation-prone
67 Y H -0.3121 Soluble
68 N H 0.0000 Buried
69 Q H -2.3948 Soluble
70 K H -2.8902 Soluble
71 F H 0.0000 Buried
72 K H -2.8930 Soluble
74 G H -1.8966 Soluble
75 R H -1.6301 Soluble
76 A H 0.0000 Buried
77 T H -0.6188 Soluble
78 M H 0.0000 Buried
79 T H -0.7436 Soluble
80 V H 0.0000 Buried
81 D H -1.2377 Soluble
82 K H -1.5582 Soluble
83 S H -0.4463 Soluble
84 I H 0.2585 Aggregation-prone
85 S H -0.4950 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.5902 Soluble
89 M H 0.0000 Buried
90 E H -1.3946 Soluble
91 L H 0.0000 Buried
92 S H -1.1228 Soluble
93 S H -1.1511 Soluble
94 L H 0.0000 Buried
95 R H -3.0642 Soluble
96 S H -2.3561 Soluble
97 E H -2.5700 Soluble
98 D H 0.0000 Buried
99 T H -0.9818 Soluble
100 A H 0.0000 Buried
101 V H 0.1579 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 E H -0.7304 Soluble
108 D H 0.0000 Buried
109 D H -2.2602 Soluble
110 H H -1.1726 Soluble
113 D H -1.2190 Soluble
114 A H 0.0000 Buried
115 F H 0.0000 Buried
116 A H -0.4319 Soluble
117 Y H -0.1595 Soluble
118 W H -0.4265 Soluble
119 G H 0.0000 Buried
120 Q H -1.3479 Soluble
121 G H -0.6707 Soluble
122 T H 0.0000 Buried
123 L H 0.0051 Aggregation-prone
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -1.2651 Soluble
128 S H -0.9997 Soluble
129 A H -0.5637 Soluble
130 S H -0.6444 Soluble
131 T H -0.7203 Soluble
132 K H -1.1928 Soluble
133 G H -1.4150 Soluble
134 P H 0.0000 Buried
135 S H -0.2328 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.1599 Soluble
139 L H 0.0000 Buried
140 A H -1.3142 Soluble
141 P H 0.0000 Buried
142 S H -1.0735 Soluble
143 S H -0.7108 Soluble
144 K H -1.0907 Soluble
145 S H 0.0000 Buried
146 T H -0.7075 Soluble
147 S H -0.7389 Soluble
148 G H -0.8370 Soluble
149 G H -0.9088 Soluble
150 T H -0.6249 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H 0.0000 Buried
159 D H -0.3639 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H 0.0000 Buried
163 E H -0.4587 Soluble
164 P H -0.8133 Soluble
165 V H -0.8628 Soluble
166 T H -0.7658 Soluble
167 V H -0.4335 Soluble
168 S H -0.4054 Soluble
169 W H 0.0000 Buried
170 N H -0.6783 Soluble
171 S H -0.5923 Soluble
172 G H -0.5208 Soluble
173 A H -0.2473 Soluble
174 L H -0.0036 Soluble
175 T H -0.1693 Soluble
176 S H -0.3654 Soluble
177 G H -0.3968 Soluble
178 V H 0.1903 Aggregation-prone
179 H H -0.3154 Soluble
180 T H -0.1744 Soluble
181 F H 0.0000 Buried
182 P H -0.3909 Soluble
183 A H 0.1348 Aggregation-prone
184 V H 0.4057 Aggregation-prone
185 L H 1.1649 Aggregation-prone
186 Q H 0.2871 Aggregation-prone
187 S H -0.0463 Soluble
188 S H -0.1792 Soluble
189 G H 0.0410 Aggregation-prone
190 L H 0.1103 Aggregation-prone
191 Y H 0.3976 Aggregation-prone
192 S H 0.0000 Buried
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.1463 Soluble
199 V H 0.0000 Buried
200 P H -0.6199 Soluble
201 S H -0.6048 Soluble
202 S H -0.5861 Soluble
203 S H -0.5996 Soluble
204 L H -0.7768 Soluble
205 G H -0.9350 Soluble
206 T H -0.6721 Soluble
207 Q H -1.1370 Soluble
208 T H -1.0782 Soluble
209 Y H 0.0000 Buried
210 I H -1.1542 Soluble
211 C H 0.0000 Buried
212 N H -1.4174 Soluble
213 V H 0.0000 Buried
214 N H -2.4333 Soluble
215 H H 0.0000 Buried
216 K H -2.8814 Soluble
217 P H -1.6955 Soluble
218 S H -1.8982 Soluble
219 N H -2.6704 Soluble
220 T H -2.2319 Soluble
221 K H -2.7654 Soluble
222 V H -1.3866 Soluble
223 D H -2.1266 Soluble
224 K H -1.8370 Soluble
225 K H -2.4320 Soluble
226 V H 0.0000 Buried
227 E H -2.8399 Soluble
228 P H -1.9515 Soluble
229 K H -2.4405 Soluble
1 D L -1.4498 Soluble
2 N L 0.0000 Buried
3 V L 0.6641 Aggregation-prone
4 L L 0.0000 Buried
5 T L -0.8509 Soluble
6 Q L 0.0000 Buried
7 S L -1.3535 Soluble
8 P L -1.2344 Soluble
9 D L -2.2822 Soluble
10 S L -1.3186 Soluble
11 L L -0.6403 Soluble
12 A L -0.4583 Soluble
13 V L 0.0000 Buried
14 S L -1.1548 Soluble
15 L L -0.3559 Soluble
16 G L -1.3938 Soluble
17 E L -2.7852 Soluble
18 R L -2.8990 Soluble
19 A L 0.0000 Buried
20 T L -0.5549 Soluble
21 I L 0.0000 Buried
22 S L -1.0759 Soluble
23 C L 0.0000 Buried
24 R L -2.2787 Soluble
25 A L 0.0000 Buried
26 S L -0.9657 Soluble
27 K L -1.7619 Soluble
28 S L -1.7232 Soluble
29 V L 0.0000 Buried
30 R L -1.9560 Soluble
31 T L -0.9382 Soluble
34 S L -0.4903 Soluble
35 G L -0.4382 Soluble
36 Y L 0.1569 Aggregation-prone
37 N L -0.6382 Soluble
38 Y L -0.5535 Soluble
39 M L 0.0000 Buried
40 H L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -1.2989 Soluble
45 K L -1.7101 Soluble
46 P L -1.4403 Soluble
47 G L -1.4610 Soluble
48 Q L -2.1658 Soluble
49 P L -1.4761 Soluble
50 P L 0.0000 Buried
51 K L -1.5810 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.0718 Soluble
56 L L 0.0000 Buried
57 A L 0.0000 Buried
65 S L -0.5195 Soluble
66 N L -0.4153 Soluble
67 L L -0.4665 Soluble
68 K L -0.7435 Soluble
69 S L -0.7087 Soluble
70 G L -0.7709 Soluble
71 V L -0.8040 Soluble
72 P L -1.1280 Soluble
74 D L -2.0413 Soluble
75 R L -1.4990 Soluble
76 F L 0.0000 Buried
77 S L -0.7680 Soluble
78 G L -0.3943 Soluble
79 S L -0.7379 Soluble
80 G L -1.0601 Soluble
83 S L -0.9203 Soluble
84 G L -1.4355 Soluble
85 T L -1.8945 Soluble
86 D L -2.0995 Soluble
87 F L 0.0000 Buried
88 T L -0.7853 Soluble
89 L L 0.0000 Buried
90 T L -0.6417 Soluble
91 I L 0.0000 Buried
92 S L -1.9221 Soluble
93 S L -1.8613 Soluble
94 L L 0.0000 Buried
95 Q L -1.2373 Soluble
96 A L -0.8212 Soluble
97 E L -1.9771 Soluble
98 D L 0.0000 Buried
99 A L -0.7392 Soluble
100 A L 0.0000 Buried
101 T L -0.9497 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 H L 0.0000 Buried
107 S L 0.0000 Buried
108 R L -1.3490 Soluble
109 E L -1.2789 Soluble
114 L L 0.2502 Aggregation-prone
115 P L 0.0000 Buried
116 Y L 0.0994 Aggregation-prone
117 T L -0.0614 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 Q L -1.2536 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -1.6159 Soluble
124 L L 0.0000 Buried
125 E L 0.0000 Buried
126 I L -0.6690 Soluble
127 K L -1.1333 Soluble
128 R L -0.7758 Soluble
129 T L 0.1186 Aggregation-prone
130 V L 0.9801 Aggregation-prone
131 A L 0.3611 Aggregation-prone
132 A L 0.0502 Aggregation-prone
133 P L 0.0000 Buried
134 S L -0.2038 Soluble
135 V L 0.0000 Buried
136 F L 0.0000 Buried
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.9565 Soluble
140 P L -0.9985 Soluble
141 S L -1.8151 Soluble
142 D L -3.0783 Soluble
143 E L -2.8348 Soluble
144 Q L 0.0000 Buried
145 L L -2.2549 Soluble
146 K L -2.8133 Soluble
147 S L -1.7458 Soluble
148 G L -1.2513 Soluble
149 T L -1.0193 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.7335 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.0463 Soluble
162 R L -1.8298 Soluble
163 E L -2.6023 Soluble
164 A L -2.0365 Soluble
165 K L -2.2397 Soluble
166 V L -1.1611 Soluble
167 Q L -0.5874 Soluble
168 W L 0.0000 Buried
169 K L -0.4905 Soluble
170 V L 0.0000 Buried
171 D L -2.0663 Soluble
172 N L -1.5420 Soluble
173 A L -0.2552 Soluble
174 L L 0.7101 Aggregation-prone
175 Q L -0.2933 Soluble
176 S L -0.7302 Soluble
177 G L -1.2703 Soluble
178 N L -1.5629 Soluble
179 S L -1.5425 Soluble
180 Q L -1.6213 Soluble
181 E L -1.9862 Soluble
182 S L -1.0101 Soluble
183 V L -0.7817 Soluble
184 T L -1.0346 Soluble
185 E L -2.1375 Soluble
186 Q L -1.7678 Soluble
187 D L -1.9463 Soluble
188 S L -1.8711 Soluble
189 K L -2.2828 Soluble
190 D L -1.6923 Soluble
191 S L -1.6995 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.8443 Soluble
199 L L 0.0000 Buried
200 T L -0.6433 Soluble
201 L L -0.7605 Soluble
202 S L -0.9940 Soluble
203 K L -2.0007 Soluble
204 A L -1.7239 Soluble
205 D L -2.3367 Soluble
206 Y L 0.0000 Buried
207 E L -3.2803 Soluble
208 K L -3.5578 Soluble
209 H L -3.2187 Soluble
210 K L -3.6993 Soluble
211 V L -2.1274 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.5146 Soluble
216 V L 0.0000 Buried
217 T L -1.1444 Soluble
218 H L 0.0000 Buried
219 Q L -1.6177 Soluble
220 G L -0.3827 Soluble
221 L L -0.1234 Soluble
222 S L -0.4119 Soluble
223 S L -0.3688 Soluble
224 P L -0.4604 Soluble
225 V L 0.2256 Aggregation-prone
226 T L -0.3165 Soluble
227 K L -0.7210 Soluble
228 S L -0.9737 Soluble
229 F L 0.0000 Buried
230 N L -2.7562 Soluble
231 R L -3.4537 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
Load structure to view sequence…
Downloads
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7922 1.6993 CSV PDB
4.5 -0.842 1.6417 CSV PDB
5.0 -0.899 1.5518 CSV PDB
5.5 -0.952 1.4442 CSV PDB
6.0 -0.9885 1.3667 CSV PDB
6.5 -0.9998 1.4038 CSV PDB
7.0 -0.9869 1.4661 CSV PDB
7.5 -0.958 1.5448 CSV PDB
8.0 -0.9201 1.6309 CSV PDB
8.5 -0.8753 1.7185 CSV PDB
9.0 -0.8231 1.8033 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH