Anzurstobart / 7st5

A4DAB022_7st5_h_l | Source PDB: 7st5 | Chains: h, l | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6667
Max Score
1.1612
Min Score
-3.3729
Total Score
-277.99
Score CDR
-0.3116
Score non-CDR
-0.7118

Calculation Parameters

Chain Sequence(s) VQLVQSGAEVKKPGASVKVSCKASGYTFRGYGISWVRQAPGQGLEWMGWISAYGGETNYAQKLQGRVTMTTDTSTSTAYMELRSLRSDDTAVYYCAREAGSSWYDFDLWGRGTLVTVSSASTKGPSVFPLAPTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSTYICNVNHKPSNTKVDKKVEPDIQMTQSPSSVSASVGDRVTITCRASQGISSWLAWYQQKPGKAPKLLIYAASNLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQGASFPITFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNR input.pdb
Selected Chain(s) h (Heavy), l (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.3729
Maximum Score 1.1612
Average Score -0.6667
Total Score -277.9948

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V h 1.0548 Aggregation-prone
3 Q h -0.3208 Soluble
4 L h 0.0000 Buried
5 V h 0.5705 Aggregation-prone
6 Q h 0.0000 Buried
7 S h -0.3145 Soluble
8 G h -0.0511 Soluble
9 A h 0.3602 Aggregation-prone
11 E h 0.1581 Aggregation-prone
12 V h 0.6857 Aggregation-prone
13 K h -1.0918 Soluble
14 K h -2.2585 Soluble
15 P h -2.3565 Soluble
16 G h -1.7599 Soluble
17 A h -1.3141 Soluble
18 S h -1.5933 Soluble
19 V h 0.0000 Buried
20 K h -1.8642 Soluble
21 V h 0.0000 Buried
22 S h -0.4360 Soluble
23 C h 0.0000 Buried
24 K h -0.8870 Soluble
25 A h 0.0000 Buried
26 S h -0.1109 Soluble
27 G h 0.0923 Aggregation-prone
28 Y h 0.0529 Aggregation-prone
29 T h -0.3124 Soluble
30 F h 0.0000 Buried
35 R h -0.9697 Soluble
36 G h -0.5042 Soluble
37 Y h 0.1326 Aggregation-prone
38 G h 0.0000 Buried
39 I h 0.0000 Buried
40 S h 0.0000 Buried
41 W h 0.0000 Buried
42 V h 0.0000 Buried
43 R h 0.0000 Buried
44 Q h -0.5491 Soluble
45 A h -0.9012 Soluble
46 P h -0.9102 Soluble
47 G h -1.2662 Soluble
48 Q h -1.7473 Soluble
49 G h -1.1725 Soluble
50 L h 0.0000 Buried
51 E h -0.7318 Soluble
52 W h 0.0000 Buried
53 M h 0.0000 Buried
54 G h 0.0000 Buried
55 W h -0.2446 Soluble
56 I h 0.0000 Buried
57 S h -0.6426 Soluble
58 A h 0.0000 Buried
59 Y h 0.2321 Aggregation-prone
62 G h -0.7864 Soluble
63 G h -1.3850 Soluble
64 E h -2.0413 Soluble
65 T h -1.2493 Soluble
66 N h -1.0371 Soluble
67 Y h -0.9347 Soluble
68 A h 0.0000 Buried
69 Q h -2.6933 Soluble
70 K h -2.6664 Soluble
71 L h 0.0000 Buried
72 Q h -2.3020 Soluble
74 G h -1.6419 Soluble
75 R h -1.4508 Soluble
76 V h 0.0000 Buried
77 T h -0.8002 Soluble
78 M h 0.0000 Buried
79 T h -0.4865 Soluble
80 T h -0.6320 Soluble
81 D h -1.1736 Soluble
82 T h -0.6921 Soluble
83 S h -0.5269 Soluble
84 T h -0.6552 Soluble
85 S h -0.9395 Soluble
86 T h 0.0000 Buried
87 A h 0.0000 Buried
88 Y h -0.5227 Soluble
89 M h 0.0000 Buried
90 E h -1.3560 Soluble
91 L h 0.0000 Buried
92 R h -1.6601 Soluble
93 S h -1.5589 Soluble
94 L h 0.0000 Buried
95 R h -3.1769 Soluble
96 S h -2.3878 Soluble
97 D h -2.5182 Soluble
98 D h 0.0000 Buried
99 T h -0.7422 Soluble
100 A h 0.0000 Buried
101 V h 0.4369 Aggregation-prone
102 Y h 0.0000 Buried
103 Y h 0.0000 Buried
104 C h 0.0000 Buried
105 A h 0.0000 Buried
106 R h -0.2531 Soluble
107 E h 0.0000 Buried
108 A h -0.0674 Soluble
109 G h -0.0186 Soluble
110 S h -0.1862 Soluble
111 S h -0.1544 Soluble
112 W h 0.2132 Aggregation-prone
113 Y h 0.3212 Aggregation-prone
114 D h 0.0000 Buried
115 F h 0.0000 Buried
116 D h -0.6702 Soluble
117 L h -0.4139 Soluble
118 W h -0.7918 Soluble
119 G h 0.0000 Buried
120 R h -1.8611 Soluble
121 G h 0.0000 Buried
122 T h 0.0000 Buried
123 L h 1.0124 Aggregation-prone
124 V h 0.0000 Buried
125 T h 0.0000 Buried
126 V h 0.0000 Buried
127 S h -1.3417 Soluble
128 S h -1.2028 Soluble
129 A h -0.6546 Soluble
130 S h -0.7632 Soluble
131 T h -0.9035 Soluble
132 K h -1.7641 Soluble
133 G h -1.7107 Soluble
134 P h 0.0000 Buried
135 S h -0.6250 Soluble
136 V h 0.0000 Buried
137 F h 0.0000 Buried
138 P h -1.0406 Soluble
139 L h 0.0000 Buried
140 A h -0.8793 Soluble
141 P h -0.6073 Soluble
142 T h -0.2927 Soluble
143 A h -0.2294 Soluble
144 A h 0.0000 Buried
145 L h 0.0000 Buried
146 G h 0.0000 Buried
147 C h 0.0000 Buried
148 L h 0.0000 Buried
149 V h 0.0000 Buried
150 K h 0.0000 Buried
151 D h -0.6709 Soluble
152 Y h 0.0000 Buried
153 F h 0.0000 Buried
154 P h 0.0000 Buried
155 E h -0.7014 Soluble
156 P h -0.9541 Soluble
157 V h -0.9338 Soluble
158 T h -0.7603 Soluble
159 V h -0.4784 Soluble
160 S h -0.5042 Soluble
161 W h 0.0000 Buried
162 N h -0.7368 Soluble
163 S h -0.6765 Soluble
164 G h -0.5703 Soluble
165 A h -0.2166 Soluble
166 L h 0.1266 Aggregation-prone
167 T h -0.1114 Soluble
168 S h -0.1395 Soluble
169 G h -0.3978 Soluble
170 V h 0.2328 Aggregation-prone
171 H h 0.0000 Buried
172 T h 0.0951 Aggregation-prone
173 F h 0.0000 Buried
174 P h -0.4199 Soluble
175 A h -0.0136 Soluble
176 V h 0.1738 Aggregation-prone
177 L h 0.6527 Aggregation-prone
178 Q h 0.0418 Aggregation-prone
179 S h -0.1924 Soluble
180 S h -0.2968 Soluble
181 G h -0.1217 Soluble
182 L h -0.2427 Soluble
183 Y h 0.0000 Buried
184 S h 0.0000 Buried
185 L h 0.0000 Buried
186 S h 0.0000 Buried
187 S h 0.0000 Buried
188 V h 0.0000 Buried
189 V h 0.0000 Buried
190 T h -0.1532 Soluble
191 V h 0.0000 Buried
192 P h -0.4486 Soluble
193 S h -0.4865 Soluble
194 S h -0.4029 Soluble
195 T h -0.5741 Soluble
196 Y h -0.6732 Soluble
197 I h -1.2904 Soluble
198 C h 0.0000 Buried
199 N h -1.6112 Soluble
200 V h 0.0000 Buried
201 N h -2.2757 Soluble
202 H h 0.0000 Buried
203 K h -2.8393 Soluble
204 P h -1.6528 Soluble
205 S h -1.9946 Soluble
206 N h -2.6262 Soluble
207 T h -2.2443 Soluble
208 K h -2.8338 Soluble
209 V h -1.6718 Soluble
210 D h -2.7143 Soluble
211 K h -2.1812 Soluble
212 K h -2.7088 Soluble
213 V h 0.0000 Buried
214 E h -2.4401 Soluble
215 P h -1.3715 Soluble
1 D l -2.2627 Soluble
2 I l -1.6057 Soluble
3 Q l -2.1925 Soluble
4 M l 0.0000 Buried
5 T l -1.3179 Soluble
6 Q l -0.9735 Soluble
7 S l -0.6479 Soluble
8 P l -0.6113 Soluble
9 S l -0.8685 Soluble
10 S l -0.8492 Soluble
11 V l -0.5655 Soluble
12 S l -1.0147 Soluble
13 A l 0.0000 Buried
14 S l -0.7236 Soluble
15 V l 0.2013 Aggregation-prone
16 G l -0.9082 Soluble
17 D l -1.9547 Soluble
18 R l -2.4532 Soluble
19 V l 0.0000 Buried
20 T l -0.6077 Soluble
21 I l 0.0000 Buried
22 T l -0.8107 Soluble
23 C l 0.0000 Buried
24 R l -2.7078 Soluble
25 A l 0.0000 Buried
26 S l -2.0356 Soluble
27 Q l -2.2541 Soluble
28 G l -1.4038 Soluble
29 I l 0.0000 Buried
36 S l -0.4793 Soluble
37 S l -0.3219 Soluble
38 W l 0.2231 Aggregation-prone
39 L l 0.0000 Buried
40 A l 0.0000 Buried
41 W l 0.0000 Buried
42 Y l 0.0000 Buried
43 Q l 0.0000 Buried
44 Q l 0.0000 Buried
45 K l -1.6132 Soluble
46 P l -1.5142 Soluble
47 G l -1.7581 Soluble
48 K l -2.7039 Soluble
49 A l -1.8482 Soluble
50 P l 0.0000 Buried
51 K l -1.6123 Soluble
52 L l 0.0000 Buried
53 L l 0.0000 Buried
54 I l 0.0000 Buried
55 Y l -0.1422 Soluble
56 A l -0.1816 Soluble
57 A l 0.0000 Buried
65 S l -0.8503 Soluble
66 N l -1.2450 Soluble
67 L l -0.5258 Soluble
68 Q l -0.6647 Soluble
69 S l -0.6054 Soluble
70 G l -0.5467 Soluble
71 V l -0.2524 Soluble
72 P l -0.3779 Soluble
74 S l -0.4331 Soluble
75 R l -0.8647 Soluble
76 F l 0.0000 Buried
77 S l -0.5845 Soluble
78 G l -0.5229 Soluble
79 S l -0.8010 Soluble
80 G l -0.9779 Soluble
83 S l -0.7997 Soluble
84 G l -1.2335 Soluble
85 T l -1.8116 Soluble
86 D l -2.1462 Soluble
87 F l 0.0000 Buried
88 T l -0.7298 Soluble
89 L l 0.0000 Buried
90 T l -0.6323 Soluble
91 I l 0.0000 Buried
92 S l -1.4207 Soluble
93 S l -1.2511 Soluble
94 L l 0.0000 Buried
95 Q l -0.7577 Soluble
96 P l -0.7434 Soluble
97 E l -1.4942 Soluble
98 D l 0.0000 Buried
99 F l -0.5354 Soluble
100 A l 0.0000 Buried
101 T l 0.0000 Buried
102 Y l 0.0000 Buried
103 Y l 0.0000 Buried
104 C l 0.0000 Buried
105 Q l 0.0000 Buried
106 Q l 0.0000 Buried
107 G l 0.0000 Buried
108 A l 0.1630 Aggregation-prone
109 S l 0.0059 Aggregation-prone
114 F l 0.0980 Aggregation-prone
115 P l -0.4203 Soluble
116 I l 0.0000 Buried
117 T l -0.6154 Soluble
118 F l 0.0000 Buried
119 G l 0.0000 Buried
120 G l -1.1780 Soluble
121 G l -1.0236 Soluble
122 T l 0.0000 Buried
123 K l -1.8568 Soluble
124 V l 0.0000 Buried
125 E l -1.1379 Soluble
126 I l -0.5000 Soluble
127 K l -1.3649 Soluble
128 R l -0.9507 Soluble
129 T l 0.1787 Aggregation-prone
130 V l 1.1612 Aggregation-prone
131 A l 0.5110 Aggregation-prone
132 A l 0.0857 Aggregation-prone
133 P l 0.0000 Buried
134 S l -0.0918 Soluble
135 V l 0.0000 Buried
136 F l 0.0683 Aggregation-prone
137 I l 0.0580 Aggregation-prone
138 F l 0.0000 Buried
139 P l -0.6352 Soluble
140 P l -0.8075 Soluble
141 S l -1.7816 Soluble
142 D l -3.2061 Soluble
143 E l -3.3729 Soluble
144 Q l 0.0000 Buried
145 L l -2.4248 Soluble
146 K l -3.0734 Soluble
147 S l -1.9091 Soluble
148 G l -1.5016 Soluble
149 T l -0.9580 Soluble
150 A l 0.0000 Buried
151 S l 0.0000 Buried
152 V l 0.0000 Buried
153 V l 0.0000 Buried
154 C l 0.0000 Buried
155 L l 0.0000 Buried
156 L l 0.0000 Buried
157 N l 0.0000 Buried
158 N l -0.6808 Soluble
159 F l 0.0000 Buried
160 Y l 0.0000 Buried
161 P l -1.1078 Soluble
162 R l -2.1190 Soluble
163 E l -2.7547 Soluble
164 A l -2.0712 Soluble
165 K l -2.2254 Soluble
166 V l -1.1251 Soluble
167 Q l -0.4854 Soluble
168 W l 0.0000 Buried
169 K l -0.4615 Soluble
170 V l 0.0000 Buried
171 D l -1.8101 Soluble
172 N l -1.5112 Soluble
173 A l -0.2444 Soluble
174 L l 0.7969 Aggregation-prone
175 Q l -0.0098 Soluble
176 S l -0.3608 Soluble
177 G l -0.9002 Soluble
178 N l -0.8517 Soluble
179 S l -1.1201 Soluble
180 Q l -1.4259 Soluble
181 E l -1.8408 Soluble
182 S l -0.7058 Soluble
183 V l -0.4735 Soluble
184 T l -0.9282 Soluble
185 E l -2.0680 Soluble
186 Q l -1.5913 Soluble
187 D l -1.7624 Soluble
188 S l -1.6687 Soluble
189 K l -2.1882 Soluble
190 D l -1.5561 Soluble
191 S l -1.5618 Soluble
192 T l 0.0000 Buried
193 Y l 0.0000 Buried
194 S l 0.0000 Buried
195 L l 0.0000 Buried
196 S l 0.0000 Buried
197 S l 0.0000 Buried
198 T l -0.6260 Soluble
199 L l 0.0000 Buried
200 T l -0.3021 Soluble
201 L l -0.4920 Soluble
202 S l -0.8314 Soluble
203 K l -1.7613 Soluble
204 A l -1.5132 Soluble
205 D l -2.0398 Soluble
206 Y l 0.0000 Buried
207 E l -3.2864 Soluble
208 K l -3.2028 Soluble
209 H l -2.9098 Soluble
210 K l -3.0763 Soluble
211 V l -1.6976 Soluble
212 Y l 0.0000 Buried
213 A l -0.7203 Soluble
214 C l 0.0000 Buried
215 E l -0.7498 Soluble
216 V l 0.0000 Buried
217 T l -1.1826 Soluble
218 H l 0.0000 Buried
219 Q l -1.3225 Soluble
220 G l -0.2055 Soluble
221 L l -0.0192 Soluble
222 S l -0.3692 Soluble
223 S l -0.3588 Soluble
224 P l -0.4379 Soluble
225 V l 0.1112 Aggregation-prone
226 T l -0.4251 Soluble
227 K l -0.7084 Soluble
228 S l -0.8070 Soluble
229 F l -1.0669 Soluble
230 N l -2.3046 Soluble
231 R l -2.6226 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7303 2.2571 CSV PDB
4.5 -0.7699 2.2045 CSV PDB
5.0 -0.8154 2.1295 CSV PDB
5.5 -0.8574 2.0445 CSV PDB
6.0 -0.8849 1.9628 CSV PDB
6.5 -0.8895 1.8978 CSV PDB
7.0 -0.8712 1.8585 CSV PDB
7.5 -0.8377 1.841 CSV PDB
8.0 -0.7958 1.8346 CSV PDB
8.5 -0.7474 1.8324 CSV PDB
9.0 -0.6933 1.8317 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH