Volagidemab / 5xez

A4DAB298_5xez_H_L | Source PDB: 5xez | Chains: H, L | Done
99.15
Yes
10 Å
Yes
Avg A4D Score
-0.7063
Max Score
2.8227
Min Score
-3.6593
Total Score
-314.29
Score CDR
-0.2622
Score non-CDR
-0.7689

Calculation Parameters

Chain Sequence(s) QVQLVESGGGVVQPGRSLRLSCAASGFTFSSYGMHWVRQAPGKGLEWVAVMYYDGSNKDYVDSVKGRFTISRDNSKNTLYLQMNRLRAEDTAVYYCAREKDHYDILTGYNYYYGLDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDIQMTQSPSSLSASVGDRVTITCRASQGIRNDLGWYQQKPGKAPKRLIYAASSLESGVPSRFSGSGSGTEFTLTISSVQPEDFVTYYCLQHNSNPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.6593
Maximum Score 2.8227
Average Score -0.7063
Total Score -314.2894

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q H -1.0299 Soluble
2 V H 0.0283 Aggregation-prone
3 Q H -0.5241 Soluble
4 L H 0.0000 Buried
5 V H 0.5050 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.5914 Soluble
8 G H -0.8317 Soluble
9 G H -0.4498 Soluble
11 G H 0.3205 Aggregation-prone
12 V H 1.0762 Aggregation-prone
13 V H -0.1379 Soluble
14 Q H -1.3544 Soluble
15 P H -2.1198 Soluble
16 G H -2.6387 Soluble
17 R H -3.0054 Soluble
18 S H -2.3524 Soluble
19 L H -1.5243 Soluble
20 R H -2.1757 Soluble
21 L H 0.0000 Buried
22 S H -0.5652 Soluble
23 C H 0.0000 Buried
24 A H -0.2638 Soluble
25 A H 0.0000 Buried
26 S H -0.4924 Soluble
27 G H -0.4736 Soluble
28 F H -0.1049 Soluble
29 T H -0.1617 Soluble
30 F H 0.0000 Buried
35 S H -0.4915 Soluble
36 S H -0.5722 Soluble
37 Y H 0.0000 Buried
38 G H 0.0000 Buried
39 M H 0.0000 Buried
40 H H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.8434 Soluble
45 A H -1.2651 Soluble
46 P H -0.9714 Soluble
47 G H -1.4688 Soluble
48 K H -2.2918 Soluble
49 G H -1.4522 Soluble
50 L H 0.0000 Buried
51 E H -1.2033 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 A H 0.0000 Buried
55 V H 0.0000 Buried
56 M H 0.0000 Buried
57 Y H -0.6910 Soluble
58 Y H -0.1939 Soluble
59 D H -1.4996 Soluble
62 G H -1.3478 Soluble
63 S H -1.4490 Soluble
64 N H -2.0478 Soluble
65 K H -1.7442 Soluble
66 D H -1.5800 Soluble
67 Y H -1.3241 Soluble
68 V H -1.4174 Soluble
69 D H -2.4584 Soluble
70 S H -1.7813 Soluble
71 V H 0.0000 Buried
72 K H -2.6257 Soluble
74 G H -1.9065 Soluble
75 R H -1.9525 Soluble
76 F H 0.0000 Buried
77 T H -1.0881 Soluble
78 I H 0.0000 Buried
79 S H -0.4312 Soluble
80 R H -0.8776 Soluble
81 D H -1.6221 Soluble
82 N H -1.6851 Soluble
83 S H -1.7079 Soluble
84 K H -2.4240 Soluble
85 N H -1.7702 Soluble
86 T H 0.0000 Buried
87 L H 0.0000 Buried
88 Y H -0.7122 Soluble
89 L H 0.0000 Buried
90 Q H -1.5015 Soluble
91 M H 0.0000 Buried
92 N H -2.5342 Soluble
93 R H -3.2281 Soluble
94 L H 0.0000 Buried
95 R H -2.5933 Soluble
96 A H -1.4978 Soluble
97 E H -2.1794 Soluble
98 D H 0.0000 Buried
99 T H -0.6002 Soluble
100 A H 0.0000 Buried
101 V H 0.1258 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H -0.4787 Soluble
107 E H 0.0000 Buried
108 K H -1.1634 Soluble
109 D H -0.5340 Soluble
110 H H -0.0428 Soluble
111 Y H 1.4624 Aggregation-prone
111A D H 1.6486 Aggregation-prone
111B I H 2.8227 Aggregation-prone
111C L H 2.6359 Aggregation-prone
111D T H 1.5590 Aggregation-prone
112D G H 1.3756 Aggregation-prone
112C Y H 1.6873 Aggregation-prone
112B N H 0.7347 Aggregation-prone
112A Y H 0.7344 Aggregation-prone
112 Y H 0.5324 Aggregation-prone
113 Y H 0.3994 Aggregation-prone
114 G H 0.0000 Buried
115 L H 0.0000 Buried
116 D H -0.5376 Soluble
117 V H -0.2867 Soluble
118 W H -0.2427 Soluble
119 G H 0.0000 Buried
120 Q H -1.3705 Soluble
121 G H -0.7272 Soluble
122 T H 0.0000 Buried
123 T H 0.0519 Aggregation-prone
124 V H 0.0000 Buried
125 T H -0.1033 Soluble
126 V H 0.0000 Buried
127 S H -0.1953 Soluble
128 S H -0.3132 Soluble
129 A H -0.2667 Soluble
130 S H -0.4050 Soluble
131 T H -0.5393 Soluble
132 K H -1.1172 Soluble
133 G H -1.3419 Soluble
134 P H 0.0000 Buried
135 S H -0.3985 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -0.9425 Soluble
139 L H 0.0000 Buried
140 A H -1.0858 Soluble
141 P H 0.0000 Buried
142 S H 0.0000 Buried
143 S H -0.9069 Soluble
144 K H -1.3706 Soluble
145 S H 0.0000 Buried
146 T H -0.7112 Soluble
147 S H -0.7098 Soluble
148 G H -0.8053 Soluble
149 G H -0.8670 Soluble
150 T H -0.5635 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H -0.3392 Soluble
159 D H -0.5143 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H -0.6319 Soluble
163 E H -1.1271 Soluble
164 P H -1.1192 Soluble
165 V H -0.8843 Soluble
166 T H -0.6491 Soluble
167 V H -0.3439 Soluble
168 S H -0.3782 Soluble
169 W H 0.0000 Buried
170 N H -0.7271 Soluble
171 S H -0.6841 Soluble
172 G H -0.5364 Soluble
173 A H -0.2419 Soluble
174 L H -0.0091 Soluble
175 T H -0.1674 Soluble
176 S H -0.1740 Soluble
177 G H -0.2151 Soluble
178 V H 0.1929 Aggregation-prone
179 H H -0.3036 Soluble
180 T H -0.1793 Soluble
181 F H 0.0000 Buried
182 P H -0.2202 Soluble
183 A H 0.2302 Aggregation-prone
184 V H 0.4846 Aggregation-prone
185 L H 1.0494 Aggregation-prone
186 Q H 0.1991 Aggregation-prone
187 S H -0.1009 Soluble
188 S H -0.2205 Soluble
189 G H -0.0004 Soluble
190 L H 0.0021 Aggregation-prone
191 Y H 0.2272 Aggregation-prone
192 S H 0.0000 Buried
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.1137 Soluble
199 V H 0.0000 Buried
200 P H -0.4681 Soluble
201 S H -0.5385 Soluble
202 S H -0.5507 Soluble
203 S H -0.6002 Soluble
204 L H -0.6804 Soluble
205 G H -1.0178 Soluble
206 T H -0.8103 Soluble
207 Q H -1.2428 Soluble
208 T H -1.1125 Soluble
209 Y H 0.0000 Buried
210 I H -1.0799 Soluble
211 C H 0.0000 Buried
212 N H -1.4297 Soluble
213 V H 0.0000 Buried
214 N H -1.9226 Soluble
215 H H 0.0000 Buried
216 K H -2.7695 Soluble
217 P H -1.6867 Soluble
218 S H -1.7992 Soluble
219 N H -2.5082 Soluble
220 T H -2.0358 Soluble
221 K H -2.6363 Soluble
222 V H -1.4945 Soluble
223 D H -2.4598 Soluble
224 K H -2.0451 Soluble
225 K H -2.1831 Soluble
226 V H 0.0000 Buried
227 E H -2.6078 Soluble
228 P H -1.9192 Soluble
229 K H -2.2744 Soluble
230 S H -1.0998 Soluble
231 C H -0.1896 Soluble
1 D L -2.4720 Soluble
2 I L 0.0000 Buried
3 Q L -2.0891 Soluble
4 M L 0.0000 Buried
5 T L -0.8820 Soluble
6 Q L -0.7550 Soluble
7 S L -0.5518 Soluble
8 P L -0.5664 Soluble
9 S L -0.7873 Soluble
10 S L -0.7613 Soluble
11 L L -0.4881 Soluble
12 S L -0.8897 Soluble
13 A L 0.0000 Buried
14 S L -0.8877 Soluble
15 V L -0.3064 Soluble
16 G L -1.0354 Soluble
17 D L -1.9015 Soluble
18 R L -2.4220 Soluble
19 V L 0.0000 Buried
20 T L -0.6157 Soluble
21 I L 0.0000 Buried
22 T L -0.7026 Soluble
23 C L 0.0000 Buried
24 R L -1.9652 Soluble
25 A L 0.0000 Buried
26 S L -1.8919 Soluble
27 Q L -2.5548 Soluble
28 G L -2.1817 Soluble
29 I L 0.0000 Buried
36 R L -2.9141 Soluble
37 N L -1.9662 Soluble
38 D L 0.0000 Buried
39 L L 0.0000 Buried
40 G L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.5142 Soluble
46 P L -1.3818 Soluble
47 G L -1.6331 Soluble
48 K L -2.5061 Soluble
49 A L -1.5715 Soluble
50 P L 0.0000 Buried
51 K L -1.4220 Soluble
52 R L -0.6101 Soluble
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.4601 Aggregation-prone
56 A L -0.0633 Soluble
57 A L 0.0000 Buried
65 S L -0.2564 Soluble
66 S L 0.1608 Aggregation-prone
67 L L 0.3560 Aggregation-prone
68 E L -0.3531 Soluble
69 S L -0.4388 Soluble
70 G L -0.5207 Soluble
71 V L -0.2341 Soluble
72 P L -0.3206 Soluble
74 S L -0.4076 Soluble
75 R L -0.7995 Soluble
76 F L 0.0000 Buried
77 S L -0.3013 Soluble
78 G L -0.1718 Soluble
79 S L -0.8330 Soluble
80 G L -1.5071 Soluble
83 S L -1.8504 Soluble
84 G L -2.2683 Soluble
85 T L -2.2418 Soluble
86 E L -2.7689 Soluble
87 F L 0.0000 Buried
88 T L -0.7943 Soluble
89 L L 0.0000 Buried
90 T L -0.6144 Soluble
91 I L 0.0000 Buried
92 S L -1.4695 Soluble
93 S L -1.2375 Soluble
94 V L 0.0000 Buried
95 Q L -0.9864 Soluble
96 P L -1.0281 Soluble
97 E L -1.9384 Soluble
98 D L 0.0000 Buried
99 F L -0.6924 Soluble
100 V L 0.0000 Buried
101 T L -0.6486 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 L L 0.0000 Buried
106 Q L 0.0000 Buried
107 H L 0.0000 Buried
108 N L -1.2017 Soluble
109 S L -1.1330 Soluble
114 N L -1.4133 Soluble
115 P L -1.5515 Soluble
116 L L 0.0000 Buried
117 T L -0.9569 Soluble
118 F L -0.4094 Soluble
119 G L 0.0000 Buried
120 G L -1.0924 Soluble
121 G L -0.9066 Soluble
122 T L 0.0000 Buried
123 K L -1.1551 Soluble
124 V L 0.0000 Buried
125 E L -1.1012 Soluble
126 I L -0.6769 Soluble
127 K L -1.5306 Soluble
128 R L -1.1499 Soluble
129 T L -0.0685 Soluble
130 V L 0.5767 Aggregation-prone
131 A L 0.0853 Aggregation-prone
132 A L -0.0493 Soluble
133 P L 0.0000 Buried
134 S L -0.2232 Soluble
135 V L 0.0000 Buried
136 F L 0.0000 Buried
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -1.1136 Soluble
140 P L 0.0000 Buried
141 S L -1.5824 Soluble
142 D L -2.6802 Soluble
143 E L -2.0546 Soluble
144 Q L 0.0000 Buried
145 L L -1.8862 Soluble
146 K L -2.5007 Soluble
147 S L -1.5345 Soluble
148 G L -1.0784 Soluble
149 T L -0.9624 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.9682 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.6496 Soluble
162 R L -2.7652 Soluble
163 E L -3.0519 Soluble
164 A L -2.2421 Soluble
165 K L -2.2729 Soluble
166 V L -1.0616 Soluble
167 Q L -0.4867 Soluble
168 W L 0.0000 Buried
169 K L -0.6072 Soluble
170 V L 0.0000 Buried
171 D L -1.9570 Soluble
172 N L -1.5645 Soluble
173 A L -0.2829 Soluble
174 L L 0.7381 Aggregation-prone
175 Q L -0.4164 Soluble
176 S L -0.7941 Soluble
177 G L -1.3567 Soluble
178 N L -1.7703 Soluble
179 S L -1.5939 Soluble
180 Q L -1.7237 Soluble
181 E L -1.9969 Soluble
182 S L -0.8903 Soluble
183 V L -0.8683 Soluble
184 T L 0.0000 Buried
185 E L -2.2416 Soluble
186 Q L -1.7800 Soluble
187 D L -1.7702 Soluble
188 S L -1.6258 Soluble
189 K L -2.1510 Soluble
190 D L -1.5723 Soluble
191 S L -1.6263 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.8494 Soluble
199 L L 0.0000 Buried
200 T L -0.6755 Soluble
201 L L -1.0352 Soluble
202 S L -1.2387 Soluble
203 K L -2.1904 Soluble
204 A L -2.0835 Soluble
205 D L -3.0238 Soluble
206 Y L 0.0000 Buried
207 E L -3.6556 Soluble
208 K L -3.6593 Soluble
209 H L -3.2034 Soluble
210 K L -3.3100 Soluble
211 V L -2.0240 Soluble
212 Y L 0.0000 Buried
213 A L -0.6855 Soluble
214 C L 0.0000 Buried
215 E L -0.5361 Soluble
216 V L 0.0000 Buried
217 T L -1.1362 Soluble
218 H L 0.0000 Buried
219 Q L -1.6541 Soluble
220 G L -0.4088 Soluble
221 L L -0.2592 Soluble
222 S L -0.4855 Soluble
223 S L -0.4212 Soluble
224 P L -0.5606 Soluble
225 V L 0.0991 Aggregation-prone
226 T L -0.2923 Soluble
227 K L -0.6514 Soluble
228 S L -0.7974 Soluble
229 F L 0.0000 Buried
230 N L -2.4612 Soluble
231 R L -2.5967 Soluble
232 G L -1.8338 Soluble
233 E L -2.5407 Soluble
234 C L -1.6096 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Downloads
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7716 4.0366 CSV PDB
4.5 -0.8137 3.9951 CSV PDB
5.0 -0.8632 3.9547 CSV PDB
5.5 -0.9102 3.9207 CSV PDB
6.0 -0.9434 3.8952 CSV PDB
6.5 -0.9533 3.872 CSV PDB
7.0 -0.9393 3.8424 CSV PDB
7.5 -0.9095 3.8061 CSV PDB
8.0 -0.8709 3.7697 CSV PDB
8.5 -0.826 3.7412 CSV PDB
9.0 -0.7746 3.7243 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH