Pamlectabart / 8qya

A4DAB201_8qya_H_L | Source PDB: 8qya | Chains: H, L | Done
98.68
Yes
10 Å
Yes
Avg A4D Score
-0.6994
Max Score
1.4937
Min Score
-3.2954
Total Score
-299.35
Score CDR
-0.2956
Score non-CDR
-0.7492

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFTFSRYWMSWVRQAPGKGLVWVGEINPNSSTINYAPSLKDKFTISRDNAKNTLYLQMNSLRAEDTAVYYCASLYYDYGDAYDYWGQGTLVTVSSASTKGPSVFPLAPSSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPAEIVMTQSPATLSVSPGERATLSCKASQSVESNVAWYQQKPGQSPRALIYSASLRFSGIPARFSGSGGTEFTLTISSLQSEDFAVYYCQQYNNYPLTFGAGTKLELKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEA input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.2954
Maximum Score 1.4937
Average Score -0.6994
Total Score -299.3479

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.0128 Soluble
2 V H -0.9510 Soluble
3 Q H -1.4718 Soluble
4 L H 0.0000 Buried
5 V H -0.0125 Soluble
6 E H -0.4969 Soluble
7 S H -0.7671 Soluble
8 G H -1.0662 Soluble
9 G H -0.5646 Soluble
11 G H -0.0764 Soluble
12 L H 0.0280 Aggregation-prone
13 V H -0.6871 Soluble
14 Q H -1.6959 Soluble
15 P H -1.7693 Soluble
16 G H -1.6180 Soluble
17 G H -1.2388 Soluble
18 S H -1.2745 Soluble
19 L H -1.2791 Soluble
20 R H -2.2522 Soluble
21 L H 0.0000 Buried
22 S H -0.6612 Soluble
23 C H 0.0000 Buried
24 A H -0.5501 Soluble
25 A H 0.0000 Buried
26 S H -1.0793 Soluble
27 G H -1.0433 Soluble
28 F H -0.6131 Soluble
29 T H -0.6984 Soluble
30 F H 0.0000 Buried
35 S H -1.6840 Soluble
36 R H -1.9285 Soluble
37 Y H 0.0000 Buried
38 W H 0.6613 Aggregation-prone
39 M H 0.0000 Buried
40 S H 0.2298 Aggregation-prone
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H -0.0164 Soluble
44 Q H -0.4171 Soluble
45 A H -0.9471 Soluble
46 P H -0.9564 Soluble
47 G H -1.4471 Soluble
48 K H -1.9779 Soluble
49 G H -0.8773 Soluble
50 L H 0.0000 Buried
51 V H 0.5819 Aggregation-prone
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 G H 0.0000 Buried
55 E H -0.0121 Soluble
56 I H 0.0000 Buried
57 N H -0.6245 Soluble
58 P H -1.2394 Soluble
59 N H -1.7365 Soluble
62 S H -0.8754 Soluble
63 S H -0.6296 Soluble
64 T H -0.2820 Soluble
65 I H 0.1903 Aggregation-prone
66 N H -0.0902 Soluble
67 Y H -0.5335 Soluble
68 A H 0.0000 Buried
69 P H -1.1391 Soluble
70 S H -1.2097 Soluble
71 L H 0.0000 Buried
72 K H -2.8772 Soluble
74 D H -3.0359 Soluble
75 K H -2.5986 Soluble
76 F H 0.0000 Buried
77 T H -1.2392 Soluble
78 I H 0.0000 Buried
79 S H -0.5323 Soluble
80 R H -1.1148 Soluble
81 D H -1.6229 Soluble
82 N H -2.0672 Soluble
83 A H -1.4052 Soluble
84 K H -2.2931 Soluble
85 N H -1.7548 Soluble
86 T H -1.0499 Soluble
87 L H 0.0000 Buried
88 Y H -0.5934 Soluble
89 L H 0.0000 Buried
90 Q H -1.5877 Soluble
91 M H 0.0000 Buried
92 N H -1.7995 Soluble
93 S H -1.6246 Soluble
94 L H 0.0000 Buried
95 R H -2.9288 Soluble
96 A H -2.0066 Soluble
97 E H -2.3970 Soluble
98 D H 0.0000 Buried
99 T H -0.7907 Soluble
100 A H 0.0000 Buried
101 V H 0.1778 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 S H 0.0000 Buried
107 L H 1.2205 Aggregation-prone
108 Y H 1.0322 Aggregation-prone
109 Y H 1.4937 Aggregation-prone
110 D H 0.8500 Aggregation-prone
111 Y H 1.1755 Aggregation-prone
112 G H 0.2706 Aggregation-prone
113 D H 0.2201 Aggregation-prone
114 A H 0.0000 Buried
115 Y H 0.6188 Aggregation-prone
116 D H 0.4214 Aggregation-prone
117 Y H 0.2490 Aggregation-prone
118 W H -0.3004 Soluble
119 G H -0.7700 Soluble
120 Q H -1.4738 Soluble
121 G H -0.7431 Soluble
122 T H -0.2207 Soluble
123 L H 0.1446 Aggregation-prone
124 V H 0.0000 Buried
125 T H -0.4824 Soluble
126 V H 0.0000 Buried
127 S H -0.8331 Soluble
128 S H -0.4615 Soluble
129 A H -0.4931 Soluble
130 S H -0.6470 Soluble
131 T H -0.9101 Soluble
132 K H -1.5296 Soluble
133 G H -1.5112 Soluble
134 P H -0.7863 Soluble
135 S H -0.4350 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.1651 Soluble
139 L H 0.0000 Buried
140 A H -0.5494 Soluble
141 P H 0.0000 Buried
142 S H -0.2726 Soluble
143 S H -0.3452 Soluble
144 G H -0.6755 Soluble
145 T H -0.5174 Soluble
146 A H 0.0000 Buried
147 A H 0.0000 Buried
148 L H 0.0000 Buried
149 G H 0.0000 Buried
150 C H 0.0000 Buried
151 L H 0.0000 Buried
152 V H 0.0000 Buried
153 K H 0.0000 Buried
154 D H -0.4473 Soluble
155 Y H 0.0000 Buried
156 F H 0.0000 Buried
157 P H 0.0000 Buried
158 E H -0.5525 Soluble
159 P H -0.6589 Soluble
160 V H -0.5869 Soluble
161 T H -0.5263 Soluble
162 V H -0.3070 Soluble
163 S H -0.4368 Soluble
164 W H 0.0000 Buried
165 N H -0.8256 Soluble
166 S H -0.7266 Soluble
167 G H -0.5982 Soluble
168 A H -0.2699 Soluble
169 L H -0.0246 Soluble
170 T H -0.1945 Soluble
171 S H -0.1763 Soluble
172 G H -0.2333 Soluble
173 V H 0.1339 Aggregation-prone
174 H H -0.3557 Soluble
175 T H -0.1068 Soluble
176 F H 0.0000 Buried
177 P H -0.1954 Soluble
178 A H 0.3357 Aggregation-prone
179 V H 0.6439 Aggregation-prone
180 L H 1.4419 Aggregation-prone
181 Q H 0.3697 Aggregation-prone
182 S H -0.0228 Soluble
183 S H -0.2102 Soluble
184 G H -0.0006 Soluble
185 L H 0.0865 Aggregation-prone
186 Y H 0.4851 Aggregation-prone
187 S H 0.0000 Buried
188 L H 0.1663 Aggregation-prone
189 S H 0.0000 Buried
190 S H 0.0000 Buried
191 V H 0.0000 Buried
192 V H 0.0000 Buried
193 T H -0.1402 Soluble
194 V H 0.0000 Buried
195 P H -0.5500 Soluble
196 S H -0.6906 Soluble
197 S H -0.6700 Soluble
198 S H -0.6315 Soluble
199 L H -0.8405 Soluble
200 G H -0.9963 Soluble
201 T H -0.7507 Soluble
202 Q H -1.2345 Soluble
203 T H -1.1790 Soluble
204 Y H 0.0000 Buried
205 I H -1.5011 Soluble
206 C H 0.0000 Buried
207 N H -1.6803 Soluble
208 V H 0.0000 Buried
209 N H -1.9069 Soluble
210 H H 0.0000 Buried
211 K H -2.6719 Soluble
212 P H -1.5858 Soluble
213 S H -1.9255 Soluble
214 N H -2.5649 Soluble
215 T H -2.2203 Soluble
216 K H -2.7178 Soluble
217 V H -1.8745 Soluble
218 D H -2.7708 Soluble
219 K H -2.4666 Soluble
220 R H -2.8347 Soluble
221 V H 0.0000 Buried
222 E H -2.4670 Soluble
223 P H -1.0937 Soluble
224 A H -0.7057 Soluble
1 E L -1.4116 Soluble
2 I L 0.0000 Buried
3 V L 0.7488 Aggregation-prone
4 M L 0.0000 Buried
5 T L -0.3711 Soluble
6 Q L 0.0000 Buried
7 S L -0.5032 Soluble
8 P L -0.2190 Soluble
9 A L -0.1705 Soluble
10 T L -0.2684 Soluble
11 L L -0.0869 Soluble
12 S L -0.4573 Soluble
13 V L 0.0000 Buried
14 S L -1.4862 Soluble
15 P L -1.7682 Soluble
16 G L -1.7452 Soluble
17 E L -2.6130 Soluble
18 R L -2.7357 Soluble
19 A L 0.0000 Buried
20 T L -0.5176 Soluble
21 L L 0.0000 Buried
22 S L -0.9090 Soluble
23 C L 0.0000 Buried
24 K L -2.2453 Soluble
25 A L -1.3894 Soluble
26 S L -1.1240 Soluble
27 Q L -2.1884 Soluble
28 S L -2.2052 Soluble
29 V L 0.0000 Buried
36 E L -2.6882 Soluble
37 S L -1.7330 Soluble
38 N L -1.2175 Soluble
39 V L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L -0.6976 Soluble
44 Q L 0.0000 Buried
45 K L -1.6193 Soluble
46 P L -1.3318 Soluble
47 G L -1.5075 Soluble
48 Q L -2.0753 Soluble
50 P L -0.8445 Soluble
51 R L -0.9950 Soluble
52 A L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.1136 Aggregation-prone
56 S L -0.3470 Soluble
57 A L 0.0000 Buried
65 S L -0.1993 Soluble
66 L L 0.3914 Aggregation-prone
67 R L -0.5696 Soluble
68 F L 0.0000 Buried
69 S L -0.2734 Soluble
70 G L -0.5529 Soluble
71 I L -0.3769 Soluble
72 P L -0.3712 Soluble
74 A L -0.3788 Soluble
75 R L -0.7054 Soluble
76 F L 0.0000 Buried
77 S L -0.3635 Soluble
78 G L 0.0000 Buried
79 S L -0.7011 Soluble
80 G L -1.6152 Soluble
84 G L -2.3160 Soluble
85 T L -2.3989 Soluble
86 E L -3.0749 Soluble
87 F L 0.0000 Buried
88 T L -0.8873 Soluble
89 L L 0.0000 Buried
90 T L -0.6067 Soluble
91 I L 0.0000 Buried
92 S L -1.4089 Soluble
93 S L -1.5692 Soluble
94 L L 0.0000 Buried
95 Q L -1.8913 Soluble
96 S L -1.7302 Soluble
97 E L -2.2512 Soluble
98 D L 0.0000 Buried
99 F L -0.7744 Soluble
100 A L 0.0000 Buried
101 V L -0.3457 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 Y L -0.0719 Soluble
108 N L -1.0906 Soluble
109 N L -0.9100 Soluble
114 Y L 0.5273 Aggregation-prone
115 P L 0.2653 Aggregation-prone
116 L L 0.5147 Aggregation-prone
117 T L 0.4034 Aggregation-prone
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 A L -0.1870 Soluble
121 G L -0.4566 Soluble
122 T L 0.0000 Buried
123 K L -0.7570 Soluble
124 L L 0.0000 Buried
125 E L -0.7044 Soluble
126 L L -1.0053 Soluble
127 K L -1.1311 Soluble
128 R L -1.0201 Soluble
129 T L -0.0451 Soluble
130 V L 0.9200 Aggregation-prone
131 A L -0.1268 Soluble
132 A L -0.0950 Soluble
133 P L 0.0000 Buried
134 S L -0.2642 Soluble
135 V L 0.0000 Buried
136 F L -0.0045 Soluble
137 I L 0.0559 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.4523 Soluble
140 P L 0.0000 Buried
141 S L -1.4708 Soluble
142 D L -2.7338 Soluble
143 E L -2.5819 Soluble
144 Q L 0.0000 Buried
145 L L -2.1436 Soluble
146 K L -2.6741 Soluble
147 S L -1.6842 Soluble
148 G L -1.3704 Soluble
149 T L -1.0196 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -1.4966 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.7476 Soluble
162 R L -3.2384 Soluble
163 E L -3.2954 Soluble
164 A L -2.4079 Soluble
165 K L -2.4801 Soluble
166 V L -1.0557 Soluble
167 Q L -0.6169 Soluble
168 W L 0.0000 Buried
169 K L -0.6739 Soluble
170 V L 0.0000 Buried
171 D L -1.8545 Soluble
172 N L -1.5504 Soluble
173 A L -0.4296 Soluble
174 L L 0.5324 Aggregation-prone
175 Q L -0.4099 Soluble
176 S L -0.7381 Soluble
177 G L -1.4037 Soluble
178 N L -1.7840 Soluble
179 S L -1.7147 Soluble
180 Q L -1.8679 Soluble
181 E L -2.0444 Soluble
182 S L -0.9423 Soluble
183 V L -0.6181 Soluble
184 T L -0.9450 Soluble
185 E L -1.7615 Soluble
186 Q L -1.9812 Soluble
187 D L -2.4077 Soluble
188 S L -2.2628 Soluble
189 K L -2.9162 Soluble
190 D L -3.0596 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.8496 Soluble
199 L L 0.0000 Buried
200 T L -0.4849 Soluble
201 L L -0.7649 Soluble
202 S L -1.1239 Soluble
203 K L -2.1672 Soluble
204 A L -1.8778 Soluble
205 D L -2.6455 Soluble
206 Y L 0.0000 Buried
207 E L -2.9496 Soluble
208 K L -3.1882 Soluble
209 H L -2.5445 Soluble
210 K L -2.3872 Soluble
211 V L -1.2588 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.8014 Soluble
216 V L 0.0000 Buried
217 T L -1.2182 Soluble
218 H L 0.0000 Buried
219 Q L -1.7520 Soluble
220 G L -0.3507 Soluble
221 L L -0.2150 Soluble
222 S L -0.4699 Soluble
223 S L -0.4015 Soluble
224 P L -0.5418 Soluble
225 V L 0.0395 Aggregation-prone
226 T L -0.5142 Soluble
227 K L -0.7741 Soluble
228 S L -0.5106 Soluble
229 F L -0.7142 Soluble
230 N L -1.5420 Soluble
231 R L -2.0902 Soluble
232 G L -1.9004 Soluble
233 E L -2.3297 Soluble
234 A L -1.1101 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6644 2.562 CSV PDB
4.5 -0.7095 2.5502 CSV PDB
5.0 -0.763 2.5342 CSV PDB
5.5 -0.8145 2.5168 CSV PDB
6.0 -0.8526 2.5005 CSV PDB
6.5 -0.8677 2.4882 CSV PDB
7.0 -0.8591 2.4812 CSV PDB
7.5 -0.8344 2.4782 CSV PDB
8.0 -0.8015 2.4771 CSV PDB
8.5 -0.7631 2.4768 CSV PDB
9.0 -0.7191 2.4766 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH