Bevacizumab / 1cz8

A4DAB044_1cz8_Y_X | Source PDB: 1cz8 | Chains: Y, X | Done
97.83
Yes
10 Å
Yes
Avg A4D Score
-0.6636
Max Score
2.4612
Min Score
-3.8691
Total Score
-286.02
Score CDR
-0.0974
Score non-CDR
-0.7379

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGYDFTHYGMNWVRQAPGKGLEWVGWINTYTGEPTYAADFKRRFTFSLDTSKSTAYLQMNSLRAEDTAVYYCAKYPYYYGTSHWYFDVWGQGTLVTVSSASTKGPSVFPLAPSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKDIQLTQSPSSLSASVGDRVTITCSASQDISNYLNWYQQKPGKAPKVLIYFTSSLHSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYSTVPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) Y (Heavy), X (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.8691
Maximum Score 2.4612
Average Score -0.6636
Total Score -286.0197

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E Y -2.0391 Soluble
2 V Y -1.3193 Soluble
3 Q Y -0.9221 Soluble
4 L Y 0.0000 Buried
5 V Y 1.1945 Aggregation-prone
6 E Y 0.0000 Buried
7 S Y -0.3716 Soluble
8 G Y -0.8730 Soluble
9 G Y -0.4480 Soluble
11 G Y -0.2296 Soluble
12 L Y 0.0091 Aggregation-prone
13 V Y -0.5721 Soluble
14 Q Y -1.5195 Soluble
15 P Y -1.5775 Soluble
16 G Y -1.3492 Soluble
17 G Y -1.0701 Soluble
18 S Y -1.2028 Soluble
19 L Y -1.0806 Soluble
20 R Y -1.9171 Soluble
21 L Y 0.0000 Buried
22 S Y -0.3047 Soluble
23 C Y 0.0000 Buried
24 A Y 0.0327 Aggregation-prone
25 A Y 0.0000 Buried
26 S Y -1.1189 Soluble
27 G Y -1.5790 Soluble
28 Y Y -1.4083 Soluble
29 D Y -1.9961 Soluble
30 F Y 0.0000 Buried
35 T Y -0.5038 Soluble
36 H Y -0.9082 Soluble
37 Y Y -0.0976 Soluble
38 G Y -0.1458 Soluble
39 M Y 0.0000 Buried
40 N Y 0.0000 Buried
41 W Y 0.0000 Buried
42 V Y 0.0000 Buried
43 R Y -0.6172 Soluble
44 Q Y -0.9396 Soluble
45 A Y -1.2546 Soluble
46 P Y -0.9795 Soluble
47 G Y -1.4963 Soluble
48 K Y -2.3868 Soluble
49 G Y -1.6772 Soluble
50 L Y 0.0000 Buried
51 E Y -1.2663 Soluble
52 W Y 0.0000 Buried
53 V Y 0.0000 Buried
54 G Y 0.0000 Buried
55 W Y -0.0942 Soluble
56 I Y 0.0000 Buried
57 N Y -0.6373 Soluble
58 T Y 0.0000 Buried
59 Y Y 0.6548 Aggregation-prone
62 T Y -0.1743 Soluble
63 G Y -0.8844 Soluble
64 E Y -1.8443 Soluble
65 P Y -1.2128 Soluble
66 T Y -0.4677 Soluble
67 Y Y -0.6954 Soluble
68 A Y -0.7932 Soluble
69 A Y -1.5952 Soluble
70 D Y -2.6283 Soluble
71 F Y 0.0000 Buried
72 K Y -3.1465 Soluble
74 R Y -3.2659 Soluble
75 R Y -2.2508 Soluble
76 F Y 0.0000 Buried
77 T Y -1.1258 Soluble
78 F Y 0.0000 Buried
79 S Y -0.5041 Soluble
80 L Y -0.6220 Soluble
81 D Y -1.1540 Soluble
82 T Y -1.0166 Soluble
83 S Y -1.2433 Soluble
84 K Y -2.0178 Soluble
85 S Y -1.3887 Soluble
86 T Y 0.0000 Buried
87 A Y 0.0000 Buried
88 Y Y 0.0000 Buried
89 L Y 0.0000 Buried
90 Q Y -1.2005 Soluble
91 M Y 0.0000 Buried
92 N Y -1.5123 Soluble
93 S Y -1.3578 Soluble
94 L Y 0.0000 Buried
95 R Y -2.1001 Soluble
96 A Y -1.6168 Soluble
97 E Y -2.1597 Soluble
98 D Y 0.0000 Buried
99 T Y -0.7150 Soluble
100 A Y 0.0000 Buried
101 V Y 0.1555 Aggregation-prone
102 Y Y 0.0000 Buried
103 Y Y 0.0000 Buried
104 C Y 0.0000 Buried
105 A Y 0.0000 Buried
106 K Y 0.0000 Buried
107 Y Y 0.0000 Buried
108 P Y 1.1064 Aggregation-prone
109 Y Y 2.0392 Aggregation-prone
110 Y Y 2.4612 Aggregation-prone
111 Y Y 1.9577 Aggregation-prone
111A G Y 0.5940 Aggregation-prone
112B T Y 0.5408 Aggregation-prone
112A S Y 0.8199 Aggregation-prone
112 H Y 0.9843 Aggregation-prone
113 W Y 0.7417 Aggregation-prone
114 Y Y 0.0000 Buried
115 F Y 0.0000 Buried
116 D Y -0.0826 Soluble
117 V Y -0.2637 Soluble
118 W Y 0.0000 Buried
119 G Y 0.0000 Buried
120 Q Y -1.2326 Soluble
121 G Y -0.5628 Soluble
122 T Y -0.0337 Soluble
123 L Y 0.0946 Aggregation-prone
124 V Y 0.0000 Buried
125 T Y -0.4011 Soluble
126 V Y 0.0000 Buried
127 S Y -0.8916 Soluble
128 S Y -0.6980 Soluble
129 A Y -0.4567 Soluble
130 S Y -0.6070 Soluble
131 T Y -0.8306 Soluble
132 K Y -1.3756 Soluble
133 G Y -1.4647 Soluble
134 P Y 0.0000 Buried
135 S Y -0.3131 Soluble
136 V Y 0.0000 Buried
137 F Y 0.0000 Buried
138 P Y -1.0087 Soluble
139 L Y 0.0000 Buried
140 A Y -1.1020 Soluble
141 P Y 0.0000 Buried
142 S Y -0.6817 Soluble
143 G Y -0.6307 Soluble
144 T Y -0.4832 Soluble
145 A Y -0.2380 Soluble
146 A Y 0.0000 Buried
147 L Y 0.0000 Buried
148 G Y 0.0000 Buried
149 C Y 0.0000 Buried
150 L Y 0.0000 Buried
151 V Y 0.0000 Buried
152 K Y 0.0000 Buried
153 D Y -0.3885 Soluble
154 Y Y 0.0000 Buried
155 F Y 0.0000 Buried
156 P Y 0.0000 Buried
157 E Y -0.4892 Soluble
158 P Y -0.7373 Soluble
159 V Y -0.6674 Soluble
160 T Y -0.5989 Soluble
161 V Y -0.3676 Soluble
162 S Y -0.3806 Soluble
163 W Y 0.0000 Buried
164 N Y -0.8458 Soluble
165 S Y -0.6691 Soluble
166 G Y -0.5831 Soluble
167 A Y -0.2462 Soluble
168 L Y 0.0238 Aggregation-prone
169 T Y -0.1842 Soluble
170 S Y -0.1989 Soluble
171 G Y -0.2723 Soluble
172 V Y 0.1684 Aggregation-prone
173 H Y -0.3411 Soluble
174 T Y 0.0153 Aggregation-prone
175 F Y 0.0000 Buried
176 P Y -0.3322 Soluble
177 A Y 0.2177 Aggregation-prone
178 V Y 0.5165 Aggregation-prone
179 L Y 1.1879 Aggregation-prone
180 Q Y 0.2918 Aggregation-prone
181 S Y -0.0543 Soluble
182 S Y -0.2305 Soluble
183 G Y -0.0023 Soluble
184 L Y 0.1345 Aggregation-prone
185 Y Y 0.4499 Aggregation-prone
186 S Y 0.0000 Buried
187 L Y 0.0000 Buried
188 S Y 0.0000 Buried
189 S Y 0.0000 Buried
190 V Y 0.0000 Buried
191 V Y 0.0000 Buried
192 T Y -0.1523 Soluble
193 V Y 0.0000 Buried
194 P Y -0.6058 Soluble
195 S Y -0.5468 Soluble
196 S Y -0.6204 Soluble
197 S Y 0.0000 Buried
198 L Y -0.8570 Soluble
199 G Y -1.0349 Soluble
200 T Y -0.8561 Soluble
201 Q Y -1.5164 Soluble
202 T Y -1.2109 Soluble
203 Y Y 0.0000 Buried
204 I Y -1.2419 Soluble
205 C Y 0.0000 Buried
206 N Y -1.4910 Soluble
207 V Y 0.0000 Buried
208 N Y -1.8729 Soluble
209 H Y 0.0000 Buried
210 K Y -2.7709 Soluble
211 P Y -1.6176 Soluble
212 S Y -1.8816 Soluble
213 N Y -2.6422 Soluble
214 T Y -2.0760 Soluble
215 K Y -2.7214 Soluble
216 V Y -1.7053 Soluble
217 D Y -2.5863 Soluble
218 K Y -2.0273 Soluble
219 K Y -2.4080 Soluble
220 V Y 0.0000 Buried
221 E Y -2.6267 Soluble
222 P Y -1.7515 Soluble
223 K Y -2.5091 Soluble
1 D X -1.9845 Soluble
2 I X 0.0000 Buried
3 Q X -2.0440 Soluble
4 L X 0.0000 Buried
5 T X -0.9044 Soluble
6 Q X 0.0000 Buried
7 S X -0.4737 Soluble
8 P X -0.5061 Soluble
9 S X -0.7902 Soluble
10 S X -0.7146 Soluble
11 L X -0.5303 Soluble
12 S X -0.7348 Soluble
13 A X 0.0000 Buried
14 S X -0.9930 Soluble
15 V X -0.3247 Soluble
16 G X -0.9249 Soluble
17 D X -1.9780 Soluble
18 R X -2.4111 Soluble
19 V X 0.0000 Buried
20 T X -0.6631 Soluble
21 I X 0.0000 Buried
22 T X -0.5816 Soluble
23 C X 0.0000 Buried
24 S X -1.4806 Soluble
25 A X -1.7557 Soluble
26 S X -2.0129 Soluble
27 Q X -2.7083 Soluble
28 D X -2.7701 Soluble
29 I X 0.0000 Buried
36 S X -1.1158 Soluble
37 N X -0.5824 Soluble
38 Y X 0.2997 Aggregation-prone
39 L X 0.0000 Buried
40 N X 0.0000 Buried
41 W X 0.0000 Buried
42 Y X 0.0000 Buried
43 Q X 0.0000 Buried
44 Q X -1.3746 Soluble
45 K X -2.0485 Soluble
46 P X -1.6872 Soluble
47 G X -1.7410 Soluble
48 K X -2.6153 Soluble
49 A X -1.6112 Soluble
50 P X 0.0000 Buried
51 K X -1.3338 Soluble
52 V X 0.0000 Buried
53 L X 0.0000 Buried
54 I X 0.0000 Buried
55 Y X 0.7239 Aggregation-prone
56 F X 1.0347 Aggregation-prone
57 T X 0.0000 Buried
65 S X 0.0404 Aggregation-prone
66 S X 0.3057 Aggregation-prone
67 L X 0.3558 Aggregation-prone
68 H X -0.2177 Soluble
69 S X -0.3560 Soluble
70 G X -0.4775 Soluble
71 V X -0.2746 Soluble
72 P X -0.3286 Soluble
74 S X -0.4167 Soluble
75 R X -0.7359 Soluble
76 F X 0.0000 Buried
77 S X -0.3155 Soluble
78 G X -0.1518 Soluble
79 S X -0.6858 Soluble
80 G X -1.1484 Soluble
83 S X -1.3029 Soluble
84 G X -1.8043 Soluble
85 T X -2.1638 Soluble
86 D X -2.5011 Soluble
87 F X 0.0000 Buried
88 T X -0.6830 Soluble
89 L X 0.0000 Buried
90 T X -0.6188 Soluble
91 I X 0.0000 Buried
92 S X -1.3750 Soluble
93 S X -1.2117 Soluble
94 L X 0.0000 Buried
95 Q X -1.0605 Soluble
96 P X -1.6451 Soluble
97 E X -2.0010 Soluble
98 D X 0.0000 Buried
99 F X 0.0000 Buried
100 A X 0.0000 Buried
101 T X -0.9016 Soluble
102 Y X 0.0000 Buried
103 Y X 0.0000 Buried
104 C X 0.0000 Buried
105 Q X 0.0000 Buried
106 Q X 0.0000 Buried
107 Y X 0.0000 Buried
108 S X -0.0971 Soluble
109 T X 0.2891 Aggregation-prone
114 V X 0.9710 Aggregation-prone
115 P X 0.2907 Aggregation-prone
116 W X 0.0000 Buried
117 T X -0.6842 Soluble
118 F X 0.0000 Buried
119 G X 0.0000 Buried
120 Q X -1.6311 Soluble
121 G X 0.0000 Buried
122 T X 0.0000 Buried
123 K X -1.3834 Soluble
124 V X 0.0000 Buried
125 E X -1.0428 Soluble
126 I X 0.0000 Buried
127 K X -1.4871 Soluble
128 R X -1.0586 Soluble
129 T X -0.2061 Soluble
130 V X 0.1072 Aggregation-prone
131 A X -0.1894 Soluble
132 A X -0.2209 Soluble
133 P X 0.0000 Buried
134 S X -0.0937 Soluble
135 V X 0.1419 Aggregation-prone
136 F X 0.1950 Aggregation-prone
137 I X 0.1245 Aggregation-prone
138 F X 0.0000 Buried
139 P X -0.4995 Soluble
140 P X 0.0000 Buried
141 S X -1.5535 Soluble
142 D X -2.7411 Soluble
143 E X -2.9118 Soluble
144 Q X 0.0000 Buried
145 L X -2.3478 Soluble
146 K X -2.9247 Soluble
147 S X -1.8376 Soluble
148 G X -1.3812 Soluble
149 T X -1.4059 Soluble
150 A X 0.0000 Buried
151 S X 0.0000 Buried
152 V X 0.0000 Buried
153 V X 0.0000 Buried
154 C X 0.0000 Buried
155 L X 0.0000 Buried
156 L X 0.0000 Buried
157 N X -0.4760 Soluble
158 N X -1.1591 Soluble
159 F X 0.0000 Buried
160 Y X 0.0000 Buried
161 P X -1.7805 Soluble
162 R X -2.9969 Soluble
163 E X -3.1729 Soluble
164 A X -2.3317 Soluble
165 K X -2.3698 Soluble
166 V X -0.9030 Soluble
167 Q X -0.5669 Soluble
168 W X 0.0000 Buried
169 K X -0.3309 Soluble
170 V X 0.0000 Buried
171 D X -1.0547 Soluble
172 N X -1.2068 Soluble
173 A X -0.2564 Soluble
174 L X 0.3938 Aggregation-prone
175 Q X -0.3619 Soluble
176 S X -0.4705 Soluble
177 G X -0.8961 Soluble
178 N X -0.7661 Soluble
179 S X -1.1416 Soluble
180 Q X -1.4272 Soluble
181 E X -1.9755 Soluble
182 S X -1.0575 Soluble
183 V X -0.8979 Soluble
184 T X -1.1466 Soluble
185 E X -2.1429 Soluble
186 Q X 0.0000 Buried
187 D X -2.0076 Soluble
188 S X -2.2520 Soluble
189 K X -2.8082 Soluble
190 D X -2.5186 Soluble
191 S X 0.0000 Buried
192 T X 0.0000 Buried
193 Y X 0.0000 Buried
194 S X 0.0000 Buried
195 L X 0.0000 Buried
196 S X 0.0000 Buried
197 S X 0.0000 Buried
198 T X -0.6985 Soluble
199 L X 0.0000 Buried
200 T X -0.3577 Soluble
201 L X -0.7870 Soluble
202 S X -1.5968 Soluble
203 K X -2.7283 Soluble
204 A X -2.3509 Soluble
205 D X -3.4376 Soluble
206 Y X 0.0000 Buried
207 E X -3.8419 Soluble
208 K X -3.8691 Soluble
209 H X -2.6569 Soluble
210 K X -2.0874 Soluble
211 V X -0.4452 Soluble
212 Y X 0.0000 Buried
213 A X 0.0000 Buried
214 C X 0.0000 Buried
215 E X -0.5798 Soluble
216 V X 0.0000 Buried
217 T X -1.1447 Soluble
218 H X 0.0000 Buried
219 Q X -1.7942 Soluble
220 G X -0.5409 Soluble
221 L X -0.3080 Soluble
222 S X -0.4730 Soluble
223 S X -0.4097 Soluble
224 P X -0.4880 Soluble
225 V X 0.3207 Aggregation-prone
226 T X -0.2442 Soluble
227 K X -0.6004 Soluble
228 S X -0.6609 Soluble
229 F X 0.0000 Buried
230 N X -1.9938 Soluble
231 R X -2.2456 Soluble
232 G X -2.0606 Soluble
233 E X -2.7837 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6676 2.8319 CSV PDB
4.5 -0.7159 2.8322 CSV PDB
5.0 -0.7721 2.8333 CSV PDB
5.5 -0.8254 2.8358 CSV PDB
6.0 -0.8637 2.8403 CSV PDB
6.5 -0.8782 2.8455 CSV PDB
7.0 -0.8695 2.8494 CSV PDB
7.5 -0.8465 2.8513 CSV PDB
8.0 -0.8161 2.8515 CSV PDB
8.5 -0.7797 2.8504 CSV PDB
9.0 -0.736 2.8459 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH