Amubarvimab / 8gx9

A4DAB014_8gx9_I_L | Source PDB: 8gx9 | Chains: I, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6955
Max Score
1.8766
Min Score
-3.8216
Total Score
-294.18
Score CDR
-0.0532
Score non-CDR
-0.77

Calculation Parameters

Chain Sequence(s) VQLVESGGGLVQPGGSLRLSCAASGITVSSNYMNWVRQAPGKGLEWVSLIYSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYHCARDLVVYGMDVWGQGTTVTVSSASTKGPSVFPLAPSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEEIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) I (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.8216
Maximum Score 1.8766
Average Score -0.6955
Total Score -294.1754

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V I 0.9414 Aggregation-prone
3 Q I -0.3728 Soluble
4 L I 0.0000 Buried
5 V I 0.2073 Aggregation-prone
6 E I -0.2884 Soluble
7 S I -0.5106 Soluble
8 G I -0.8859 Soluble
9 G I -0.4026 Soluble
11 G I 0.2467 Aggregation-prone
12 L I 1.0849 Aggregation-prone
13 V I 0.0000 Buried
14 Q I -1.4908 Soluble
15 P I -1.8096 Soluble
16 G I -1.5921 Soluble
17 G I -1.0426 Soluble
18 S I -1.1973 Soluble
19 L I -0.9549 Soluble
20 R I -2.1293 Soluble
21 L I 0.0000 Buried
22 S I -0.6074 Soluble
23 C I 0.0000 Buried
24 A I -0.2653 Soluble
25 A I -0.3320 Soluble
26 S I -0.1842 Soluble
27 G I -0.0342 Soluble
28 I I 0.0000 Buried
29 T I -0.6506 Soluble
30 V I 0.0000 Buried
35 S I -0.9615 Soluble
36 S I -0.5301 Soluble
37 N I -0.0759 Soluble
38 Y I 0.3146 Aggregation-prone
39 M I 0.0000 Buried
40 N I 0.0000 Buried
41 W I 0.0000 Buried
42 V I 0.0000 Buried
43 R I 0.0000 Buried
44 Q I -0.8258 Soluble
45 A I -1.2163 Soluble
46 P I -0.9850 Soluble
47 G I -1.4684 Soluble
48 K I -2.4126 Soluble
49 G I -1.6279 Soluble
50 L I 0.0000 Buried
51 E I -1.2211 Soluble
52 W I 0.0000 Buried
53 V I 0.0000 Buried
54 S I 0.0000 Buried
55 L I 0.0000 Buried
56 I I 0.0000 Buried
57 Y I 0.0691 Aggregation-prone
58 S I -0.3373 Soluble
59 G I -0.6185 Soluble
63 G I -0.5267 Soluble
64 S I -0.1840 Soluble
65 T I 0.1527 Aggregation-prone
66 Y I 0.2126 Aggregation-prone
67 Y I -0.5811 Soluble
68 A I -1.3057 Soluble
69 D I -2.3986 Soluble
70 S I -1.8326 Soluble
71 V I 0.0000 Buried
72 K I -2.5291 Soluble
74 G I -1.7604 Soluble
75 R I -1.5353 Soluble
76 F I 0.0000 Buried
77 T I -0.8647 Soluble
78 I I 0.0000 Buried
79 S I -0.6370 Soluble
80 R I -1.5619 Soluble
81 D I -2.4759 Soluble
82 N I -2.6237 Soluble
83 S I -2.0686 Soluble
84 K I -2.6786 Soluble
85 N I -1.9832 Soluble
86 T I 0.0000 Buried
87 L I 0.0000 Buried
88 Y I -0.8029 Soluble
89 L I 0.0000 Buried
90 Q I -1.5193 Soluble
91 M I 0.0000 Buried
92 N I -1.5452 Soluble
93 S I -1.3566 Soluble
94 L I 0.0000 Buried
95 R I -2.8153 Soluble
96 A I -1.9747 Soluble
97 E I -2.3628 Soluble
98 D I 0.0000 Buried
99 T I -0.6951 Soluble
100 A I 0.0000 Buried
101 V I 0.1245 Aggregation-prone
102 Y I 0.0000 Buried
103 H I 0.0000 Buried
104 C I 0.0000 Buried
105 A I 0.0000 Buried
106 R I -0.0913 Soluble
107 D I 0.0000 Buried
108 L I 1.4395 Aggregation-prone
109 V I 1.3801 Aggregation-prone
110 V I 1.8646 Aggregation-prone
113 Y I 1.8766 Aggregation-prone
114 G I 0.0000 Buried
115 M I 0.0000 Buried
116 D I -0.4473 Soluble
117 V I -0.2209 Soluble
118 W I -0.4695 Soluble
119 G I 0.0000 Buried
120 Q I -1.5056 Soluble
121 G I -0.7301 Soluble
122 T I -0.3336 Soluble
123 T I -0.0431 Soluble
124 V I 0.0000 Buried
125 T I -0.2493 Soluble
126 V I 0.0000 Buried
127 S I -0.9263 Soluble
128 S I -0.8313 Soluble
129 A I -0.5352 Soluble
130 S I -0.8763 Soluble
131 T I -1.2339 Soluble
132 K I -1.9853 Soluble
133 G I -1.6258 Soluble
134 P I 0.0000 Buried
135 S I -0.5179 Soluble
136 V I 0.0000 Buried
137 F I 0.0000 Buried
138 P I -1.0979 Soluble
139 L I 0.0000 Buried
140 A I -0.8346 Soluble
141 P I -0.4104 Soluble
142 S I -0.2721 Soluble
143 G I -0.6034 Soluble
144 T I -0.4502 Soluble
145 A I -0.1767 Soluble
146 A I 0.0000 Buried
147 L I 0.0000 Buried
148 G I 0.0000 Buried
149 C I 0.0000 Buried
150 L I 0.0000 Buried
151 V I 0.0000 Buried
152 K I 0.0000 Buried
153 D I -0.6678 Soluble
154 Y I 0.0000 Buried
155 F I 0.0000 Buried
156 P I -0.7672 Soluble
157 E I -1.7260 Soluble
158 P I -1.3668 Soluble
159 V I -0.8253 Soluble
160 T I -0.4829 Soluble
161 V I -0.1121 Soluble
162 S I -0.3694 Soluble
163 W I 0.0000 Buried
164 N I -0.8051 Soluble
165 S I -0.6582 Soluble
166 G I -0.4822 Soluble
167 A I -0.2014 Soluble
168 L I 0.0239 Aggregation-prone
169 T I -0.1491 Soluble
170 S I -0.1830 Soluble
171 G I -0.1986 Soluble
172 V I 0.1841 Aggregation-prone
173 H I 0.0000 Buried
174 T I 0.1480 Aggregation-prone
175 F I 0.0000 Buried
176 P I -0.2284 Soluble
177 A I 0.0848 Aggregation-prone
178 V I 0.2823 Aggregation-prone
179 L I 0.9280 Aggregation-prone
180 Q I 0.0669 Aggregation-prone
181 S I -0.1225 Soluble
182 S I -0.2435 Soluble
183 G I -0.0525 Soluble
184 L I -0.1838 Soluble
185 Y I 0.2374 Aggregation-prone
186 S I 0.0000 Buried
187 L I 0.1430 Aggregation-prone
188 S I 0.0000 Buried
189 S I 0.0000 Buried
190 V I 0.0000 Buried
191 V I 0.0000 Buried
192 T I -0.1474 Soluble
193 V I 0.0000 Buried
194 P I -0.4012 Soluble
195 S I -0.3888 Soluble
196 S I -0.4643 Soluble
197 S I -0.4132 Soluble
198 L I -0.4398 Soluble
199 G I -0.7868 Soluble
200 T I -0.6244 Soluble
201 Q I -1.1194 Soluble
202 T I -1.0271 Soluble
203 Y I 0.0000 Buried
204 I I -1.2993 Soluble
205 C I 0.0000 Buried
206 N I -1.4543 Soluble
207 V I 0.0000 Buried
208 N I -1.8555 Soluble
209 H I 0.0000 Buried
210 K I -2.8057 Soluble
211 P I -1.9032 Soluble
212 S I -2.0029 Soluble
213 N I -2.4134 Soluble
214 T I -2.1581 Soluble
215 K I -2.6779 Soluble
216 V I -1.6379 Soluble
217 D I -2.5653 Soluble
218 K I -2.2302 Soluble
219 K I -2.5634 Soluble
220 V I 0.0000 Buried
221 E I -2.3180 Soluble
1 E L -1.5404 Soluble
2 I L -0.1155 Soluble
3 V L 0.8313 Aggregation-prone
4 L L 0.0000 Buried
5 T L -0.8113 Soluble
6 Q L 0.0000 Buried
7 S L -0.8467 Soluble
8 P L -0.4333 Soluble
9 G L -0.7936 Soluble
10 T L -0.2866 Soluble
11 L L -0.0320 Soluble
12 S L -0.4242 Soluble
13 L L -0.9773 Soluble
14 S L -1.4250 Soluble
15 P L -1.8012 Soluble
16 G L -2.1542 Soluble
17 E L -2.9125 Soluble
18 R L -3.1135 Soluble
19 A L 0.0000 Buried
20 T L -0.5336 Soluble
21 L L 0.0000 Buried
22 S L -0.8684 Soluble
23 C L 0.0000 Buried
24 R L -2.2163 Soluble
25 A L -1.0459 Soluble
26 S L -0.9677 Soluble
27 Q L -1.5383 Soluble
28 S L -1.1782 Soluble
29 V L -0.7849 Soluble
30 S L -0.3447 Soluble
36 S L -0.2650 Soluble
37 S L 0.4764 Aggregation-prone
38 Y L 0.9347 Aggregation-prone
39 L L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -0.9051 Soluble
45 K L -1.4370 Soluble
46 P L -1.2663 Soluble
47 G L -1.4358 Soluble
48 Q L -1.9279 Soluble
49 A L -1.3502 Soluble
50 P L 0.0000 Buried
51 R L -1.1939 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.4371 Aggregation-prone
56 G L 0.4295 Aggregation-prone
57 A L -0.1667 Soluble
65 S L -0.6727 Soluble
66 S L -0.6630 Soluble
67 R L -1.7545 Soluble
68 A L -0.8280 Soluble
69 T L -0.7340 Soluble
70 G L -0.9364 Soluble
71 I L 0.0000 Buried
72 P L -1.4030 Soluble
74 D L -2.4462 Soluble
75 R L -2.1220 Soluble
76 F L 0.0000 Buried
77 S L -0.8691 Soluble
78 G L 0.0000 Buried
79 S L -0.5885 Soluble
80 G L -0.9489 Soluble
83 S L -1.0964 Soluble
84 G L -1.1470 Soluble
85 T L -1.6912 Soluble
86 D L -2.0359 Soluble
87 F L 0.0000 Buried
88 T L -0.7423 Soluble
89 L L 0.0000 Buried
90 T L -0.7452 Soluble
91 I L 0.0000 Buried
92 S L -2.4105 Soluble
93 R L -3.3965 Soluble
94 L L 0.0000 Buried
95 E L -2.1507 Soluble
96 P L -1.4294 Soluble
97 E L -2.1194 Soluble
98 D L 0.0000 Buried
99 F L -0.4811 Soluble
100 A L 0.0000 Buried
101 V L 0.0697 Aggregation-prone
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 Y L 0.0000 Buried
108 G L -0.7158 Soluble
114 S L -0.5726 Soluble
115 S L -0.2727 Soluble
116 P L 0.0000 Buried
117 T L -0.0885 Soluble
118 F L 0.0489 Aggregation-prone
119 G L 0.0000 Buried
120 Q L -1.5167 Soluble
121 G L -1.0153 Soluble
122 T L 0.0000 Buried
123 K L -0.3340 Soluble
124 L L 0.0000 Buried
125 E L 0.0000 Buried
126 I L -0.7752 Soluble
127 K L -1.9703 Soluble
128 R L -2.3538 Soluble
129 T L -0.8565 Soluble
130 V L 0.1217 Aggregation-prone
131 A L -0.0767 Soluble
132 A L -0.1077 Soluble
133 P L -0.1121 Soluble
134 S L -0.0750 Soluble
135 V L 0.0000 Buried
136 F L 0.0779 Aggregation-prone
137 I L 0.0495 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.7332 Soluble
140 P L 0.0000 Buried
141 S L -1.4433 Soluble
142 D L -2.7048 Soluble
143 E L -2.4774 Soluble
144 Q L 0.0000 Buried
145 L L -2.1039 Soluble
146 K L -2.6200 Soluble
147 S L -1.6368 Soluble
148 G L -1.1878 Soluble
149 T L -0.9767 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L -0.2796 Soluble
158 N L -0.6973 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.4348 Soluble
162 R L -1.6090 Soluble
163 E L -2.6465 Soluble
164 A L -2.0344 Soluble
165 K L -2.1196 Soluble
166 V L -1.0603 Soluble
167 Q L -0.4787 Soluble
168 W L 0.0000 Buried
169 K L -0.5398 Soluble
170 V L 0.0000 Buried
171 D L -1.8068 Soluble
172 N L -1.5139 Soluble
173 A L -0.2472 Soluble
174 L L 0.7908 Aggregation-prone
175 Q L -0.1553 Soluble
176 S L -0.3771 Soluble
177 G L -0.9684 Soluble
178 N L -0.8988 Soluble
179 S L -1.3216 Soluble
180 Q L -1.9014 Soluble
181 E L -2.0705 Soluble
182 S L -0.6938 Soluble
183 V L -0.1856 Soluble
184 T L -0.7563 Soluble
185 E L -1.9003 Soluble
186 Q L -1.6203 Soluble
187 D L -1.8890 Soluble
188 S L -1.7865 Soluble
189 K L -2.5435 Soluble
190 D L -2.5769 Soluble
191 S L -2.1973 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.8181 Soluble
199 L L 0.0000 Buried
200 T L -0.3588 Soluble
201 L L -0.6962 Soluble
202 S L -1.3916 Soluble
203 K L -2.2979 Soluble
204 A L -2.1025 Soluble
205 D L -2.9693 Soluble
206 Y L 0.0000 Buried
207 E L -3.8216 Soluble
208 K L -3.8151 Soluble
209 H L -3.0722 Soluble
210 K L -3.1842 Soluble
211 V L -1.2572 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.8555 Soluble
216 V L 0.0000 Buried
217 T L -1.1312 Soluble
218 H L 0.0000 Buried
219 Q L -1.4172 Soluble
220 G L -0.4261 Soluble
221 L L -0.2308 Soluble
222 S L -0.4454 Soluble
223 S L -0.4304 Soluble
224 P L -0.5651 Soluble
225 V L -0.0442 Soluble
226 T L -0.5026 Soluble
227 K L -0.7803 Soluble
228 S L -0.5758 Soluble
229 F L -0.8825 Soluble
230 N L -1.8505 Soluble
231 R L -2.4948 Soluble
232 G L -2.2372 Soluble
233 E L -2.1842 Soluble
234 C L -0.4337 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.713 1.9575 CSV PDB
4.5 -0.7583 1.9209 CSV PDB
5.0 -0.8121 1.8839 CSV PDB
5.5 -0.865 1.8467 CSV PDB
6.0 -0.9062 1.8097 CSV PDB
6.5 -0.9259 1.7732 CSV PDB
7.0 -0.922 1.7383 CSV PDB
7.5 -0.9012 1.7186 CSV PDB
8.0 -0.8702 1.7183 CSV PDB
8.5 -0.8317 1.7172 CSV PDB
9.0 -0.7867 1.714 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH