Matuzumab / 5vsi

A4DAB167_5vsi_H_L | Source PDB: 5vsi | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7266
Max Score
1.4989
Min Score
-3.5972
Total Score
-313.9
Score CDR
-0.5606
Score non-CDR
-0.7469

Calculation Parameters

Chain Sequence(s) VQLVQSGAEVKKPGASVKVSCKASGYTFTSHWMHWVRQAPGQGLEWIGEFNPSNGRTNYNEKFKSKATMTVDTSTNTAYMELSSLRSEDTAVYYCASRDYDYDGRYFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVADYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLASVVTVPSSSLGTQTYICNVNHKPSNTKVDEKVEPDIQMTQSPSSLSASVGDRVTITCSASSSVTYMYWYQQKPGKAPKLLIYDTSNLASGVPSRFSGSGSGTDYTFTISSLQPEDIATYYCQQWSSHIFTFGQGTKVEIKRTVAAPSVFIFPPSDKQLKSGTARVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLISTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.5972
Maximum Score 1.4989
Average Score -0.7266
Total Score -313.9032

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V H 0.6592 Aggregation-prone
3 Q H -0.5400 Soluble
4 L H 0.0000 Buried
5 V H 0.2375 Aggregation-prone
6 Q H 0.0000 Buried
7 S H -0.5954 Soluble
8 G H -0.9028 Soluble
9 A H -1.0846 Soluble
11 E H -1.7262 Soluble
12 V H -1.1110 Soluble
13 K H -1.8195 Soluble
14 K H -2.4860 Soluble
15 P H -2.2372 Soluble
16 G H -1.6461 Soluble
17 A H -1.2743 Soluble
18 S H -1.3339 Soluble
19 V H 0.0000 Buried
20 K H -1.9232 Soluble
21 V H 0.0000 Buried
22 S H -0.4659 Soluble
23 C H 0.0000 Buried
24 K H -0.9944 Soluble
25 A H 0.0000 Buried
26 S H -0.4752 Soluble
27 G H -0.2077 Soluble
28 Y H -0.0392 Soluble
29 T H -0.1110 Soluble
30 F H 0.0000 Buried
35 T H -0.5006 Soluble
36 S H -0.3818 Soluble
37 H H -0.1840 Soluble
38 W H -0.1782 Soluble
39 M H 0.0000 Buried
40 H H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H -0.5420 Soluble
44 Q H -0.7584 Soluble
45 A H -0.9932 Soluble
46 P H -0.8499 Soluble
47 G H -1.2228 Soluble
48 Q H -1.8413 Soluble
49 G H -1.3749 Soluble
50 L H 0.0000 Buried
51 E H -1.0533 Soluble
52 W H 0.0000 Buried
53 I H 0.0000 Buried
54 G H 0.0000 Buried
55 E H 0.0000 Buried
56 F H -0.6201 Soluble
57 N H -1.2060 Soluble
58 P H 0.0000 Buried
59 S H -1.2855 Soluble
62 N H -2.1356 Soluble
63 G H -1.8740 Soluble
64 R H -2.4722 Soluble
65 T H -1.1678 Soluble
66 N H -1.1089 Soluble
67 Y H -1.3170 Soluble
68 N H -2.1115 Soluble
69 E H -3.3038 Soluble
70 K H -3.2192 Soluble
71 F H 0.0000 Buried
72 K H -3.0184 Soluble
74 S H -1.8138 Soluble
75 K H -1.5940 Soluble
76 A H 0.0000 Buried
77 T H -0.8823 Soluble
78 M H 0.0000 Buried
79 T H -0.5211 Soluble
80 V H -0.3752 Soluble
81 D H -1.0366 Soluble
82 T H -0.7160 Soluble
83 S H -0.6165 Soluble
84 T H -0.7696 Soluble
85 N H -1.0304 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.5397 Soluble
89 M H 0.0000 Buried
90 E H -1.5346 Soluble
91 L H 0.0000 Buried
92 S H -1.1113 Soluble
93 S H -1.2120 Soluble
94 L H 0.0000 Buried
95 R H -3.0222 Soluble
96 S H -2.2343 Soluble
97 E H -2.2308 Soluble
98 D H 0.0000 Buried
99 T H -1.0321 Soluble
100 A H 0.0000 Buried
101 V H -0.1151 Soluble
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 S H 0.0000 Buried
107 R H 0.0000 Buried
108 D H -0.1257 Soluble
109 Y H -0.3818 Soluble
110 D H -0.9122 Soluble
111 Y H -0.0611 Soluble
112A D H -1.7356 Soluble
112 G H -1.5552 Soluble
113 R H -2.2314 Soluble
114 Y H -0.9019 Soluble
115 F H -0.1945 Soluble
116 D H 0.0079 Aggregation-prone
117 Y H 0.5115 Aggregation-prone
118 W H -0.1635 Soluble
119 G H 0.0000 Buried
120 Q H -1.4347 Soluble
121 G H -0.6970 Soluble
122 T H 0.0000 Buried
123 L H -0.3142 Soluble
124 V H 0.0000 Buried
125 T H -1.1085 Soluble
126 V H 0.0000 Buried
127 S H -1.3768 Soluble
128 S H -1.2014 Soluble
129 A H -0.5938 Soluble
130 S H -0.7328 Soluble
131 T H -0.8842 Soluble
132 K H -1.3179 Soluble
133 G H -1.4641 Soluble
134 P H -0.7839 Soluble
135 S H -0.4065 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -0.7969 Soluble
139 L H 0.0000 Buried
140 A H -0.9377 Soluble
141 P H 0.0000 Buried
142 S H -0.7753 Soluble
143 S H -0.7166 Soluble
144 K H -1.0962 Soluble
145 S H 0.0000 Buried
146 T H -0.6692 Soluble
147 S H -0.6466 Soluble
148 G H -0.7839 Soluble
149 G H -0.8626 Soluble
150 T H -0.5925 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 A H 0.0000 Buried
159 D H -0.5651 Soluble
160 Y H 0.0000 Buried
161 F H -0.4002 Soluble
162 P H 0.0000 Buried
163 E H -0.9117 Soluble
164 P H -0.8911 Soluble
165 V H 0.0000 Buried
166 T H -0.6303 Soluble
167 V H -0.5096 Soluble
168 S H -0.4624 Soluble
169 W H 0.0000 Buried
170 N H -0.8422 Soluble
171 S H -0.7157 Soluble
172 G H -0.5946 Soluble
173 A H -0.2801 Soluble
174 L H -0.0943 Soluble
175 T H -0.2564 Soluble
176 S H -0.2861 Soluble
177 G H -0.4267 Soluble
178 V H 0.0491 Aggregation-prone
179 H H -0.3146 Soluble
180 T H -0.1360 Soluble
181 F H 0.0000 Buried
182 P H -0.2549 Soluble
183 A H 0.2344 Aggregation-prone
184 V H 0.6388 Aggregation-prone
185 L H 1.4989 Aggregation-prone
186 Q H 0.3720 Aggregation-prone
187 S H -0.0179 Soluble
188 S H -0.2081 Soluble
189 G H 0.1310 Aggregation-prone
190 L H 0.0841 Aggregation-prone
191 Y H 0.3843 Aggregation-prone
192 S H 0.0000 Buried
193 L H 0.0000 Buried
194 A H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.1846 Soluble
199 V H 0.0000 Buried
200 P H -0.6152 Soluble
201 S H -0.6052 Soluble
202 S H -0.5829 Soluble
203 S H -0.5853 Soluble
204 L H -0.8418 Soluble
205 G H -1.0037 Soluble
206 T H -0.7610 Soluble
207 Q H -1.3199 Soluble
208 T H -1.2166 Soluble
209 Y H 0.0000 Buried
210 I H -1.3560 Soluble
211 C H 0.0000 Buried
212 N H -1.5934 Soluble
213 V H 0.0000 Buried
214 N H -1.9388 Soluble
215 H H 0.0000 Buried
216 K H -2.4217 Soluble
217 P H -1.7214 Soluble
218 S H -1.7682 Soluble
219 N H -2.3863 Soluble
220 T H -2.0212 Soluble
221 K H -2.6932 Soluble
222 V H -1.9169 Soluble
223 D H -2.6579 Soluble
224 E H -2.1258 Soluble
225 K H -2.5505 Soluble
226 V H 0.0000 Buried
227 E H -2.5037 Soluble
228 P H -1.3748 Soluble
1 D L -1.9629 Soluble
2 I L -1.2524 Soluble
3 Q L -1.5997 Soluble
4 M L 0.0000 Buried
5 T L -0.8358 Soluble
6 Q L 0.0000 Buried
7 S L -0.6267 Soluble
8 P L -0.6441 Soluble
9 S L -0.9158 Soluble
10 S L -0.8659 Soluble
11 L L -0.4409 Soluble
12 S L -0.8312 Soluble
13 A L -0.9746 Soluble
14 S L -0.9259 Soluble
15 V L -0.1762 Soluble
16 G L -0.9489 Soluble
17 D L -1.9857 Soluble
18 R L -2.4358 Soluble
19 V L 0.0000 Buried
20 T L -0.6582 Soluble
21 I L 0.0000 Buried
22 T L -0.5049 Soluble
23 C L 0.0000 Buried
24 S L -1.0875 Soluble
25 A L -0.9310 Soluble
26 S L -1.0892 Soluble
27 S L -0.9724 Soluble
28 S L -0.5203 Soluble
29 V L -0.0456 Soluble
37 T L -0.0212 Soluble
38 Y L 0.2628 Aggregation-prone
39 M L 0.0000 Buried
40 Y L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.8768 Soluble
46 P L -1.5665 Soluble
47 G L -1.8079 Soluble
48 K L -2.5718 Soluble
49 A L -1.5572 Soluble
50 P L 0.0000 Buried
51 K L -1.5095 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.0000 Buried
56 D L -0.7970 Soluble
57 T L -0.7091 Soluble
65 S L -0.9211 Soluble
66 N L -1.2703 Soluble
67 L L -0.3593 Soluble
68 A L -0.5800 Soluble
69 S L -0.6293 Soluble
70 G L -0.5173 Soluble
71 V L 0.0000 Buried
72 P L -0.3780 Soluble
74 S L -0.4126 Soluble
75 R L -0.7953 Soluble
76 F L 0.0000 Buried
77 S L -0.4677 Soluble
78 G L -0.5829 Soluble
79 S L -0.9579 Soluble
80 G L -1.2770 Soluble
83 S L -1.1936 Soluble
84 G L -1.1786 Soluble
85 T L -1.2086 Soluble
86 D L -1.8793 Soluble
87 Y L 0.0000 Buried
88 T L -0.6526 Soluble
89 F L 0.0000 Buried
90 T L -0.6042 Soluble
91 I L 0.0000 Buried
92 S L -1.4021 Soluble
93 S L -1.2592 Soluble
94 L L 0.0000 Buried
95 Q L -1.0154 Soluble
96 P L -1.6148 Soluble
97 E L -2.1011 Soluble
98 D L 0.0000 Buried
99 I L 0.0000 Buried
100 A L 0.0000 Buried
101 T L -1.0022 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 W L 0.2212 Aggregation-prone
108 S L -0.3398 Soluble
109 S L -0.7530 Soluble
114 H L -0.8842 Soluble
115 I L -0.5465 Soluble
116 F L 0.0000 Buried
117 T L -0.3786 Soluble
118 F L -0.3623 Soluble
119 G L 0.0000 Buried
120 Q L -1.6397 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -1.7375 Soluble
124 V L 0.0000 Buried
125 E L -1.0781 Soluble
126 I L 0.0000 Buried
127 K L -1.2041 Soluble
128 R L -0.7888 Soluble
129 T L 0.0358 Aggregation-prone
130 V L 0.7528 Aggregation-prone
131 A L 0.1435 Aggregation-prone
132 A L -0.0722 Soluble
133 P L 0.0000 Buried
134 S L -0.2214 Soluble
135 V L 0.0000 Buried
136 F L 0.0000 Buried
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -1.0020 Soluble
140 P L -0.8775 Soluble
141 S L -1.4620 Soluble
142 D L -2.6650 Soluble
143 K L -2.2989 Soluble
144 Q L 0.0000 Buried
145 L L -2.0542 Soluble
146 K L -2.6444 Soluble
147 S L -1.6429 Soluble
148 G L -1.3408 Soluble
149 T L -0.9781 Soluble
150 A L 0.0000 Buried
151 R L -0.2977 Soluble
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.9718 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.3712 Soluble
162 R L -2.2686 Soluble
163 E L -2.8305 Soluble
164 A L -2.0847 Soluble
165 K L -2.3663 Soluble
166 V L -1.2268 Soluble
167 Q L -0.9148 Soluble
168 W L 0.0000 Buried
169 K L -0.8640 Soluble
170 V L 0.0000 Buried
171 D L -2.1249 Soluble
172 N L -1.7189 Soluble
173 A L -0.5670 Soluble
174 L L 0.0623 Aggregation-prone
175 Q L -0.4141 Soluble
176 S L -0.5581 Soluble
177 G L -0.8566 Soluble
178 N L -0.7515 Soluble
179 S L -1.1214 Soluble
180 Q L -1.2072 Soluble
181 E L -1.8133 Soluble
182 S L -0.7770 Soluble
183 V L -0.4570 Soluble
184 T L 0.0000 Buried
185 E L -1.8523 Soluble
186 Q L 0.0000 Buried
187 D L -1.9588 Soluble
188 S L -2.2038 Soluble
189 K L -2.5843 Soluble
190 D L -1.8632 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 I L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.7264 Soluble
199 L L 0.0000 Buried
200 T L -0.5075 Soluble
201 L L -0.7080 Soluble
202 S L -1.0541 Soluble
203 K L -2.0467 Soluble
204 A L -1.8292 Soluble
205 D L -2.4835 Soluble
206 Y L 0.0000 Buried
207 E L -3.4243 Soluble
208 K L -3.5972 Soluble
209 H L -3.0882 Soluble
210 K L -3.2159 Soluble
211 V L -1.6047 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.7753 Soluble
216 V L 0.0000 Buried
217 T L -1.2161 Soluble
218 H L 0.0000 Buried
219 Q L -1.6033 Soluble
220 G L -0.3186 Soluble
221 L L -0.1729 Soluble
222 S L -0.4485 Soluble
223 S L -0.3923 Soluble
224 P L -0.5697 Soluble
225 V L 0.1120 Aggregation-prone
226 T L -0.3978 Soluble
227 K L -0.7432 Soluble
228 S L -0.6794 Soluble
229 F L 0.0000 Buried
230 N L -2.2204 Soluble
231 R L -2.7658 Soluble
232 G L -2.2882 Soluble
233 E L -2.4365 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.8311 1.8566 CSV PDB
4.5 -0.8747 1.8453 CSV PDB
5.0 -0.9258 1.8304 CSV PDB
5.5 -0.9738 1.8136 CSV PDB
6.0 -1.0064 1.7961 CSV PDB
6.5 -1.0139 1.7784 CSV PDB
7.0 -0.9966 1.7607 CSV PDB
7.5 -0.9636 1.7431 CSV PDB
8.0 -0.9222 1.7257 CSV PDB
8.5 -0.8744 1.7092 CSV PDB
9.0 -0.8203 1.6949 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH