Denecimig / 7ahu

A4DAB083_7ahu_H_L | Source PDB: 7ahu | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7416
Max Score
1.3637
Min Score
-3.79
Total Score
-324.82
Score CDR
-0.4396
Score non-CDR
-0.7796

Calculation Parameters

Chain Sequence(s) EVQLVQSGAEVKKPGESLRISCKGSGYSFSTSWIVWVRQMPGKGLEWMGMIDPSDSFTSYSPSFQGHVTISADKSISTAYLQWSSLKASDTAMYYCARLHYYNSEEFDVWGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESKYEIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGQSSRTRGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQFGDSQLFTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.79
Maximum Score 1.3637
Average Score -0.7416
Total Score -324.8226

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.0162 Soluble
2 V H -1.0150 Soluble
3 Q H -1.2851 Soluble
4 L H 0.0000 Buried
5 V H 0.0500 Aggregation-prone
6 Q H 0.0000 Buried
7 S H -0.4785 Soluble
8 G H -0.3899 Soluble
9 A H 0.2289 Aggregation-prone
11 E H -0.1589 Soluble
12 V H 0.1893 Aggregation-prone
13 K H -1.3066 Soluble
14 K H -2.2287 Soluble
15 P H -2.0725 Soluble
16 G H -1.7659 Soluble
17 E H -1.6741 Soluble
18 S H -1.4620 Soluble
19 L H 0.0000 Buried
20 R H -1.6766 Soluble
21 I H 0.0000 Buried
22 S H -0.5409 Soluble
23 C H 0.0000 Buried
24 K H -0.8433 Soluble
25 G H 0.0000 Buried
26 S H -0.7009 Soluble
27 G H -1.1328 Soluble
28 Y H -0.4587 Soluble
29 S H -0.3901 Soluble
30 F H 0.0000 Buried
35 S H -1.0034 Soluble
36 T H -0.2512 Soluble
37 S H 0.0000 Buried
38 W H 0.0294 Aggregation-prone
39 I H 0.0000 Buried
40 V H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H -0.9799 Soluble
44 Q H -1.0042 Soluble
45 M H -0.9305 Soluble
46 P H -0.9002 Soluble
47 G H -1.4237 Soluble
48 K H -2.4145 Soluble
49 G H -1.7513 Soluble
50 L H 0.0000 Buried
51 E H -1.6774 Soluble
52 W H 0.0000 Buried
53 M H 0.0000 Buried
54 G H 0.0000 Buried
55 M H 0.0000 Buried
56 I H 0.0000 Buried
57 D H -0.2614 Soluble
58 P H 0.0000 Buried
59 S H -1.2247 Soluble
62 D H -1.6799 Soluble
63 S H -0.5491 Soluble
64 F H 1.1984 Aggregation-prone
65 T H 0.6786 Aggregation-prone
66 S H 0.3086 Aggregation-prone
67 Y H -0.2592 Soluble
68 S H -0.5627 Soluble
69 P H -0.7682 Soluble
70 S H -0.8476 Soluble
71 F H 0.0000 Buried
72 Q H -1.5604 Soluble
74 G H -1.1959 Soluble
75 H H -1.1423 Soluble
76 V H 0.0000 Buried
77 T H -0.6028 Soluble
78 I H 0.0000 Buried
79 S H -0.3074 Soluble
80 A H -0.7749 Soluble
81 D H -1.4041 Soluble
82 K H -1.9992 Soluble
83 S H -0.3180 Soluble
84 I H 0.9187 Aggregation-prone
85 S H -0.3602 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.5346 Soluble
89 L H 0.0000 Buried
90 Q H -1.0088 Soluble
91 W H 0.0000 Buried
92 S H -0.8754 Soluble
93 S H -1.3549 Soluble
94 L H 0.0000 Buried
95 K H -2.3533 Soluble
96 A H -1.3104 Soluble
97 S H -0.7518 Soluble
98 D H 0.0000 Buried
99 T H -0.1916 Soluble
100 A H 0.0000 Buried
101 M H 0.1277 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H -0.5410 Soluble
107 L H 0.0000 Buried
108 H H -0.9110 Soluble
109 Y H 0.1636 Aggregation-prone
110 Y H 0.6343 Aggregation-prone
111 N H -0.7870 Soluble
112 S H -0.6870 Soluble
113 E H -0.6635 Soluble
114 E H 0.0000 Buried
115 F H 0.0000 Buried
116 D H -1.0418 Soluble
117 V H -0.7238 Soluble
118 W H -0.6837 Soluble
119 G H 0.0000 Buried
120 Q H -1.1123 Soluble
121 G H 0.0000 Buried
122 T H 0.0000 Buried
123 L H 0.9289 Aggregation-prone
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -1.0192 Soluble
128 S H -1.1082 Soluble
129 A H -0.6261 Soluble
130 S H -0.6873 Soluble
131 T H -0.6319 Soluble
132 K H -1.2441 Soluble
133 G H -1.3904 Soluble
134 P H -0.6412 Soluble
135 S H -0.3303 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.1778 Soluble
139 L H 0.0000 Buried
140 A H -0.5739 Soluble
141 P H -0.0658 Soluble
142 C H -0.5029 Soluble
143 S H -0.4565 Soluble
144 R H -0.6056 Soluble
145 S H -0.6347 Soluble
146 T H -1.2225 Soluble
147 S H -1.4577 Soluble
148 E H -2.1703 Soluble
149 S H -1.2880 Soluble
150 T H -0.8405 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H 0.0000 Buried
159 D H -0.4887 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H 0.0000 Buried
163 E H -0.9446 Soluble
164 P H -1.0559 Soluble
165 V H -0.8965 Soluble
166 T H -0.6813 Soluble
167 V H -0.3396 Soluble
168 S H -0.4647 Soluble
169 W H 0.0000 Buried
170 N H -0.9856 Soluble
171 S H -0.7640 Soluble
172 G H -0.5830 Soluble
173 A H -0.2677 Soluble
174 L H -0.0983 Soluble
175 T H -0.2177 Soluble
176 S H -0.2300 Soluble
177 G H -0.2590 Soluble
178 V H 0.1003 Aggregation-prone
179 H H -0.0991 Soluble
180 T H 0.0190 Aggregation-prone
181 F H 0.0000 Buried
182 P H -0.4062 Soluble
183 A H 0.0999 Aggregation-prone
184 V H 0.5058 Aggregation-prone
185 L H 1.0846 Aggregation-prone
186 Q H 0.2844 Aggregation-prone
187 S H -0.0798 Soluble
188 S H -0.2118 Soluble
189 G H 0.0409 Aggregation-prone
190 L H -0.0094 Soluble
191 Y H 0.2831 Aggregation-prone
192 S H 0.2141 Aggregation-prone
193 L H 0.1204 Aggregation-prone
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.1646 Soluble
199 V H 0.0000 Buried
200 P H -0.5468 Soluble
201 S H -0.8545 Soluble
202 S H -0.7876 Soluble
203 S H 0.0000 Buried
204 L H -0.9904 Soluble
205 G H -1.1103 Soluble
206 T H -1.0539 Soluble
207 K H -1.9062 Soluble
208 T H -1.6197 Soluble
209 Y H 0.0000 Buried
210 T H 0.0000 Buried
211 C H 0.0000 Buried
212 N H -1.5087 Soluble
213 V H 0.0000 Buried
214 D H -2.0137 Soluble
215 H H 0.0000 Buried
216 K H -2.8527 Soluble
217 P H -1.7599 Soluble
218 S H -1.8751 Soluble
219 N H -2.6033 Soluble
220 T H -2.1505 Soluble
221 K H -2.6886 Soluble
222 V H -1.6426 Soluble
223 D H -2.4884 Soluble
224 K H -2.2584 Soluble
225 R H -2.8085 Soluble
226 V H 0.0000 Buried
227 E H -2.7569 Soluble
228 S H -1.3506 Soluble
229 K H -1.4960 Soluble
230 Y H 0.1440 Aggregation-prone
1 E L -1.7514 Soluble
2 I L 0.0000 Buried
3 V L 0.7730 Aggregation-prone
4 L L 0.0000 Buried
5 T L -0.8205 Soluble
6 Q L 0.0000 Buried
7 S L -0.8931 Soluble
8 P L -0.6339 Soluble
9 G L -0.8691 Soluble
10 T L -0.4079 Soluble
11 L L -0.2326 Soluble
12 S L -0.5487 Soluble
13 L L -1.0019 Soluble
14 S L -1.6678 Soluble
15 P L -2.0992 Soluble
16 G L -2.4011 Soluble
17 E L -2.9415 Soluble
18 R L -3.1463 Soluble
19 A L 0.0000 Buried
20 T L -0.5669 Soluble
21 L L 0.0000 Buried
22 S L -1.0695 Soluble
23 C L 0.0000 Buried
24 R L -2.6273 Soluble
25 A L 0.0000 Buried
26 S L -1.0121 Soluble
27 Q L -1.3076 Soluble
28 S L -0.1693 Soluble
29 V L 1.3637 Aggregation-prone
30 S L 0.0081 Aggregation-prone
36 S L -0.6294 Soluble
37 S L -0.4952 Soluble
38 Y L -0.6610 Soluble
39 L L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -1.1633 Soluble
45 K L -1.5742 Soluble
46 P L -1.2818 Soluble
47 G L -1.4018 Soluble
48 Q L -2.0708 Soluble
49 A L -1.5085 Soluble
50 P L 0.0000 Buried
51 R L -2.1484 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.6301 Soluble
56 G L -1.0174 Soluble
57 Q L -1.5805 Soluble
65 S L -1.2059 Soluble
66 S L -1.3427 Soluble
67 R L -2.1953 Soluble
68 T L -1.8607 Soluble
69 R L -2.3367 Soluble
70 G L -1.6875 Soluble
71 I L -1.3394 Soluble
72 P L -1.4744 Soluble
74 D L -2.1163 Soluble
75 R L -1.8006 Soluble
76 F L 0.0000 Buried
77 S L -0.9989 Soluble
78 G L -1.0363 Soluble
79 S L -1.1891 Soluble
80 G L -1.7294 Soluble
83 S L -1.5806 Soluble
84 G L -1.5352 Soluble
85 T L -2.1717 Soluble
86 D L -2.9752 Soluble
87 F L 0.0000 Buried
88 T L -1.1007 Soluble
89 L L 0.0000 Buried
90 T L -0.8941 Soluble
91 I L 0.0000 Buried
92 S L -2.4688 Soluble
93 R L -3.3403 Soluble
94 L L 0.0000 Buried
95 E L -2.4208 Soluble
96 P L -1.8490 Soluble
97 E L -2.3677 Soluble
98 D L 0.0000 Buried
99 F L 0.0000 Buried
100 A L 0.0000 Buried
101 V L -0.1902 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 F L -0.4994 Soluble
108 G L 0.0000 Buried
109 D L -2.0855 Soluble
113 S L -1.3963 Soluble
114 Q L -1.4071 Soluble
115 L L -0.6972 Soluble
116 F L 0.0000 Buried
117 T L -0.1607 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 Q L -1.5810 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -0.7070 Soluble
124 L L 0.0000 Buried
125 E L 0.0000 Buried
126 I L -0.8208 Soluble
127 K L -1.4046 Soluble
128 R L -0.8578 Soluble
129 T L 0.2233 Aggregation-prone
130 V L 0.9784 Aggregation-prone
131 A L 0.4845 Aggregation-prone
132 A L 0.0531 Aggregation-prone
133 P L 0.0000 Buried
134 S L -0.0969 Soluble
135 V L 0.0000 Buried
136 F L 0.2338 Aggregation-prone
137 I L 0.0917 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.5387 Soluble
140 P L 0.0000 Buried
141 S L -1.5171 Soluble
142 D L -2.8421 Soluble
143 E L -3.1386 Soluble
144 Q L 0.0000 Buried
145 L L -2.3914 Soluble
146 K L -2.9283 Soluble
147 S L -1.8492 Soluble
148 G L -1.3892 Soluble
149 T L -1.2691 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L -0.3226 Soluble
158 N L -0.8689 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.6540 Soluble
162 R L -3.1172 Soluble
163 E L -3.3285 Soluble
164 A L -2.3728 Soluble
165 K L -2.4086 Soluble
166 V L -1.0083 Soluble
167 Q L -0.5635 Soluble
168 W L 0.0000 Buried
169 K L -0.6609 Soluble
170 V L 0.0000 Buried
171 D L -2.0651 Soluble
172 N L -1.6661 Soluble
173 A L -0.5297 Soluble
174 L L 0.1859 Aggregation-prone
175 Q L -0.3357 Soluble
176 S L -0.5330 Soluble
177 G L -0.8697 Soluble
178 N L -0.7966 Soluble
179 S L -1.0595 Soluble
180 Q L -1.2831 Soluble
181 E L -1.8535 Soluble
182 S L -0.7735 Soluble
183 V L -0.8320 Soluble
184 T L -1.2093 Soluble
185 E L -2.3053 Soluble
186 Q L -2.1169 Soluble
187 D L -2.0266 Soluble
188 S L -1.9998 Soluble
189 K L -2.3011 Soluble
190 D L -1.7198 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.5985 Soluble
199 L L 0.0000 Buried
200 T L -0.4278 Soluble
201 L L -0.7550 Soluble
202 S L -1.3715 Soluble
203 K L -2.3078 Soluble
204 A L -2.0618 Soluble
205 D L -2.6084 Soluble
206 Y L 0.0000 Buried
207 E L -3.7900 Soluble
208 K L -3.7561 Soluble
209 H L -3.1977 Soluble
210 K L -3.3277 Soluble
211 V L -1.4774 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.6742 Soluble
216 V L 0.0000 Buried
217 T L -1.1788 Soluble
218 H L 0.0000 Buried
219 Q L -1.7396 Soluble
220 G L -0.4141 Soluble
221 L L -0.1821 Soluble
222 S L -0.4659 Soluble
223 S L -0.4131 Soluble
224 P L -0.4894 Soluble
225 V L 0.0776 Aggregation-prone
226 T L -0.4064 Soluble
227 K L -0.7266 Soluble
228 S L -0.6042 Soluble
229 F L 0.0000 Buried
230 N L -1.9363 Soluble
231 R L -2.8001 Soluble
232 G L -2.3791 Soluble
233 E L -2.3618 Soluble
234 C L -1.1714 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.8057 3.526 CSV PDB
4.5 -0.8554 3.3781 CSV PDB
5.0 -0.9144 3.2253 CSV PDB
5.5 -0.9712 3.071 CSV PDB
6.0 -1.0127 2.9165 CSV PDB
6.5 -1.0294 2.7629 CSV PDB
7.0 -1.0206 2.6125 CSV PDB
7.5 -0.9947 2.4717 CSV PDB
8.0 -0.959 2.3537 CSV PDB
8.5 -0.9166 2.2744 CSV PDB
9.0 -0.8676 2.2345 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH