Olokizumab / 4cni

A4DAB190_4cni_H_L | Source PDB: 4cni | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.734
Max Score
1.8791
Min Score
-3.6605
Total Score
-317.81
Score CDR
-0.4436
Score non-CDR
-0.7693

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFNFNDYFMNWVRQAPGKGLEWVAQMRNKNYQYGTYYAESLEGRFTISRDDSKNSLYLQMNSLKTEDTAVYYCARESYYGFTSYWGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVEDIQMTQSPSSLSASVGDRVTITCQASQDIGISLSWYQQKPGKAPKLLIYNANNLADGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCLQHNSAPYTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.6605
Maximum Score 1.8791
Average Score -0.734
Total Score -317.8052

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.0506 Soluble
2 V H -1.1942 Soluble
3 Q H -1.0855 Soluble
4 L H 0.0000 Buried
5 V H 0.4485 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.4352 Soluble
8 G H -0.9943 Soluble
9 G H -0.5749 Soluble
11 G H -0.0470 Soluble
12 L H 0.0851 Aggregation-prone
13 V H 0.0000 Buried
14 Q H -1.6280 Soluble
15 P H -1.6961 Soluble
16 G H -1.4091 Soluble
17 G H -1.0749 Soluble
18 S H -1.3462 Soluble
19 L H -1.3322 Soluble
20 R H -2.3056 Soluble
21 L H 0.0000 Buried
22 S H -0.6016 Soluble
23 C H 0.0000 Buried
24 A H -0.3426 Soluble
25 A H 0.0000 Buried
26 S H -1.2256 Soluble
27 G H -1.4150 Soluble
28 F H -1.1938 Soluble
29 N H -1.7087 Soluble
30 F H 0.0000 Buried
35 N H -2.1208 Soluble
36 D H -1.8118 Soluble
37 Y H -0.7321 Soluble
38 F H 0.1736 Aggregation-prone
39 M H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.7799 Soluble
45 A H -1.1917 Soluble
46 P H -0.9736 Soluble
47 G H -1.5199 Soluble
48 K H -2.3659 Soluble
49 G H -1.7922 Soluble
50 L H 0.0000 Buried
51 E H -1.0836 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 A H 0.0000 Buried
55 Q H 0.0000 Buried
56 M H 0.0000 Buried
57 R H -0.5180 Soluble
58 N H 0.0000 Buried
59 K H -2.3971 Soluble
60 N H -1.9490 Soluble
61 Y H -0.3061 Soluble
62 Q H -1.3642 Soluble
63 Y H -0.3983 Soluble
64 G H -0.1550 Soluble
65 T H 0.3320 Aggregation-prone
66 Y H 0.2478 Aggregation-prone
67 Y H -0.4148 Soluble
68 A H -1.4702 Soluble
69 E H -2.8003 Soluble
70 S H -1.7707 Soluble
71 L H 0.0000 Buried
72 E H -2.7073 Soluble
74 G H -1.8201 Soluble
75 R H -1.5133 Soluble
76 F H 0.0000 Buried
77 T H -0.6211 Soluble
78 I H 0.0000 Buried
79 S H -0.3934 Soluble
80 R H -0.9381 Soluble
81 D H -1.6824 Soluble
82 D H -2.0743 Soluble
83 S H -1.5918 Soluble
84 K H -2.4349 Soluble
85 N H -1.9915 Soluble
86 S H 0.0000 Buried
87 L H 0.0000 Buried
88 Y H -0.7569 Soluble
89 L H 0.0000 Buried
90 Q H -1.6006 Soluble
91 M H 0.0000 Buried
92 N H -1.3413 Soluble
93 S H -1.1750 Soluble
94 L H 0.0000 Buried
95 K H -2.2747 Soluble
96 T H -1.8085 Soluble
97 E H -2.2628 Soluble
98 D H 0.0000 Buried
99 T H -0.7107 Soluble
100 A H 0.0000 Buried
101 V H 0.2345 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.2492 Aggregation-prone
107 E H 0.0000 Buried
108 S H 1.2044 Aggregation-prone
109 Y H 1.8791 Aggregation-prone
110 Y H 1.8329 Aggregation-prone
113 G H 0.0000 Buried
114 F H 0.0000 Buried
115 T H 0.0000 Buried
116 S H 0.4612 Aggregation-prone
117 Y H 0.5050 Aggregation-prone
118 W H -0.1217 Soluble
119 G H 0.0000 Buried
120 Q H -1.3619 Soluble
121 G H 0.0000 Buried
122 T H -0.0321 Soluble
123 L H 0.3444 Aggregation-prone
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -0.9914 Soluble
128 S H -0.7410 Soluble
129 A H -0.4775 Soluble
130 S H -0.5541 Soluble
131 T H -0.5362 Soluble
132 K H -1.0904 Soluble
133 G H -1.3696 Soluble
134 P H 0.0000 Buried
135 S H -0.4859 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.3055 Soluble
139 L H 0.0000 Buried
140 A H -0.7526 Soluble
141 P H -0.6441 Soluble
142 C H -0.6314 Soluble
143 S H -1.2678 Soluble
144 R H -2.0293 Soluble
145 S H -1.6227 Soluble
146 T H -1.1863 Soluble
147 S H -1.3073 Soluble
148 E H -2.0934 Soluble
149 S H -1.1848 Soluble
150 T H -0.7834 Soluble
151 A H -0.7455 Soluble
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H -0.3357 Soluble
159 D H -0.4120 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H 0.0000 Buried
163 E H -0.4874 Soluble
164 P H -0.7589 Soluble
165 V H -0.7484 Soluble
166 T H -0.6482 Soluble
167 V H -0.2415 Soluble
168 S H -0.5066 Soluble
169 W H 0.0000 Buried
170 N H -0.9731 Soluble
171 S H -0.7566 Soluble
172 G H -0.5739 Soluble
173 A H -0.2398 Soluble
174 L H -0.0360 Soluble
175 T H -0.1572 Soluble
176 S H -0.1539 Soluble
177 G H -0.1432 Soluble
178 V H 0.2013 Aggregation-prone
179 H H -0.2704 Soluble
180 T H 0.0606 Aggregation-prone
181 F H 0.0000 Buried
182 P H -0.2941 Soluble
183 A H 0.2655 Aggregation-prone
184 V H 0.6830 Aggregation-prone
185 L H 1.4826 Aggregation-prone
186 Q H 0.4585 Aggregation-prone
187 S H 0.0171 Aggregation-prone
188 S H -0.1251 Soluble
189 G H 0.1249 Aggregation-prone
190 L H 0.1891 Aggregation-prone
191 Y H 0.4826 Aggregation-prone
192 S H 0.0000 Buried
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.0854 Soluble
199 V H 0.0000 Buried
200 P H -0.5948 Soluble
201 S H -1.0222 Soluble
202 S H -0.5882 Soluble
203 S H -0.7015 Soluble
204 L H -1.1040 Soluble
205 G H -1.2344 Soluble
206 T H -1.1322 Soluble
207 K H -1.8577 Soluble
208 T H -1.6614 Soluble
209 Y H 0.0000 Buried
210 T H -1.9270 Soluble
211 C H 0.0000 Buried
212 N H -1.7273 Soluble
213 V H 0.0000 Buried
214 D H -2.0936 Soluble
215 H H 0.0000 Buried
216 K H -2.7885 Soluble
217 P H -1.6033 Soluble
218 S H -1.8275 Soluble
219 N H -2.6257 Soluble
220 T H -2.1231 Soluble
221 K H -2.7431 Soluble
222 V H -1.8185 Soluble
223 D H -2.8011 Soluble
224 K H -2.3412 Soluble
225 R H -2.9012 Soluble
226 V H 0.0000 Buried
227 E H -2.3616 Soluble
1 D L -2.6322 Soluble
2 I L 0.0000 Buried
3 Q L -2.1241 Soluble
4 M L 0.0000 Buried
5 T L -1.2990 Soluble
6 Q L 0.0000 Buried
7 S L -0.6398 Soluble
8 P L -0.6059 Soluble
9 S L -0.8831 Soluble
10 S L -0.7150 Soluble
11 L L -0.4631 Soluble
12 S L -0.8395 Soluble
13 A L 0.0000 Buried
14 S L -1.2543 Soluble
15 V L -0.5609 Soluble
16 G L -1.2603 Soluble
17 D L -2.4876 Soluble
18 R L -2.6205 Soluble
19 V L 0.0000 Buried
20 T L -0.4796 Soluble
21 I L 0.0000 Buried
22 T L -0.8159 Soluble
23 C L 0.0000 Buried
24 Q L -2.5776 Soluble
25 A L 0.0000 Buried
26 S L -2.2886 Soluble
27 Q L -2.9354 Soluble
28 D L -2.8886 Soluble
29 I L 0.0000 Buried
36 G L -0.9100 Soluble
37 I L 0.0799 Aggregation-prone
38 S L 0.0258 Aggregation-prone
39 L L 0.0000 Buried
40 S L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.8181 Soluble
46 P L -1.6558 Soluble
47 G L -1.7094 Soluble
48 K L -2.5418 Soluble
49 A L -1.4433 Soluble
50 P L 0.0000 Buried
51 K L -1.1006 Soluble
52 L L -0.2561 Soluble
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.2386 Aggregation-prone
56 N L -0.0138 Soluble
57 A L 0.0000 Buried
65 N L -1.3599 Soluble
66 N L -1.0703 Soluble
67 L L -0.4994 Soluble
68 A L -0.9580 Soluble
69 D L -1.8779 Soluble
70 G L -1.2113 Soluble
71 V L -0.7238 Soluble
72 P L -0.4916 Soluble
74 S L -0.3831 Soluble
75 R L -0.8347 Soluble
76 F L 0.0000 Buried
77 S L -0.6592 Soluble
78 G L -0.8048 Soluble
79 S L -1.0161 Soluble
80 G L -1.1493 Soluble
83 S L -1.0179 Soluble
84 G L -1.6783 Soluble
85 T L -2.3000 Soluble
86 D L -2.7450 Soluble
87 F L 0.0000 Buried
88 T L -0.9300 Soluble
89 L L 0.0000 Buried
90 T L -0.5966 Soluble
91 I L 0.0000 Buried
92 S L -1.5068 Soluble
93 S L -1.5699 Soluble
94 L L 0.0000 Buried
95 Q L -1.1297 Soluble
96 P L -1.2068 Soluble
97 E L -1.7873 Soluble
98 D L 0.0000 Buried
99 F L -0.6124 Soluble
100 A L 0.0000 Buried
101 T L -0.7405 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 L L 0.0000 Buried
106 Q L 0.0000 Buried
107 H L 0.0000 Buried
108 N L -0.7374 Soluble
109 S L -0.7069 Soluble
114 A L -0.3648 Soluble
115 P L -0.6841 Soluble
116 Y L 0.0000 Buried
117 T L -0.9223 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 Q L -1.7857 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -1.5204 Soluble
124 L L 0.0000 Buried
125 E L 0.0000 Buried
126 I L -0.9384 Soluble
127 K L -1.6416 Soluble
128 R L -0.9799 Soluble
129 T L 0.1285 Aggregation-prone
130 V L 1.0753 Aggregation-prone
131 A L 0.4547 Aggregation-prone
132 A L 0.1570 Aggregation-prone
133 P L 0.0000 Buried
134 S L -0.0821 Soluble
135 V L 0.0000 Buried
136 F L -0.0413 Soluble
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.3346 Soluble
140 P L 0.0000 Buried
141 S L -1.5942 Soluble
142 D L -2.9576 Soluble
143 E L -3.0283 Soluble
144 Q L 0.0000 Buried
145 L L -2.0936 Soluble
146 K L -2.8361 Soluble
147 S L -1.7295 Soluble
148 G L -1.2119 Soluble
149 T L -1.0300 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.6798 Soluble
159 F L 0.0000 Buried
160 Y L -0.6492 Soluble
161 P L -1.1501 Soluble
162 R L -1.8633 Soluble
163 E L -2.7517 Soluble
164 A L -2.1022 Soluble
165 K L -2.2453 Soluble
166 V L -1.2366 Soluble
167 Q L -0.5953 Soluble
168 W L 0.0000 Buried
169 K L -0.5282 Soluble
170 V L 0.0000 Buried
171 D L -1.7774 Soluble
172 N L -1.4459 Soluble
173 A L -0.1967 Soluble
174 L L 0.8078 Aggregation-prone
175 Q L -0.0201 Soluble
176 S L -0.3767 Soluble
177 G L -0.8265 Soluble
178 N L -0.7029 Soluble
179 S L -0.8787 Soluble
180 Q L -0.9342 Soluble
181 E L -1.4260 Soluble
182 S L -0.5145 Soluble
183 V L -0.4336 Soluble
184 T L -1.0006 Soluble
185 E L -2.1690 Soluble
186 Q L -1.8481 Soluble
187 D L -2.1743 Soluble
188 S L -2.1121 Soluble
189 K L -2.4931 Soluble
190 D L -2.0733 Soluble
191 S L -1.9656 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L 0.0000 Buried
199 L L 0.0000 Buried
200 T L -0.4092 Soluble
201 L L -0.7603 Soluble
202 S L -1.1820 Soluble
203 K L -2.1734 Soluble
204 A L -2.0590 Soluble
205 D L -2.8863 Soluble
206 Y L 0.0000 Buried
207 E L -3.5678 Soluble
208 K L -3.6605 Soluble
209 H L -2.8747 Soluble
210 K L -2.9509 Soluble
211 V L -1.4470 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.9688 Soluble
216 V L 0.0000 Buried
217 T L -1.2206 Soluble
218 H L 0.0000 Buried
219 Q L -1.4715 Soluble
220 G L -0.2052 Soluble
221 L L -0.1077 Soluble
222 S L -0.4076 Soluble
223 S L -0.3980 Soluble
224 P L -0.5682 Soluble
225 V L -0.1003 Soluble
226 T L -0.5497 Soluble
227 K L -0.7947 Soluble
228 S L -0.6491 Soluble
229 F L 0.0000 Buried
230 N L -1.6014 Soluble
231 R L -2.1461 Soluble
232 G L -1.7801 Soluble
233 E L -2.0883 Soluble
234 C L -0.5177 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
Load structure to view sequence…
Downloads
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7263 2.072 CSV PDB
4.5 -0.7792 2.0538 CSV PDB
5.0 -0.8414 2.0308 CSV PDB
5.5 -0.902 2.0057 CSV PDB
6.0 -0.949 1.9797 CSV PDB
6.5 -0.9727 1.9534 CSV PDB
7.0 -0.9718 1.927 CSV PDB
7.5 -0.9538 1.9121 CSV PDB
8.0 -0.9259 1.9104 CSV PDB
8.5 -0.8901 1.9049 CSV PDB
9.0 -0.8463 1.8878 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH