Crenezumab / 5vzy

A4DAB074_5vzy_H_L | Source PDB: 5vzy | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6949
Max Score
1.4816
Min Score
-3.608
Total Score
-294.65
Score CDR
-0.3004
Score non-CDR
-0.7406

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFTFSSYGMSWVRQAPGKGLELVASINSNGGSTYYPDSVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCASGDYWGQGTTVTVSSASTKGPSVFPLAPGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKDIVMTQSPLSLPVTPGEPASISCRSSQSLVYSNGDTYLHWYLQKPGQSPQLLIYKVSNRFSGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCSQSTHVPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.608
Maximum Score 1.4816
Average Score -0.6949
Total Score -294.6494

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -1.9364 Soluble
2 V H -0.9614 Soluble
3 Q H -0.9328 Soluble
4 L H 0.0000 Buried
5 V H 0.4006 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.4453 Soluble
8 G H -0.8172 Soluble
9 G H -0.4744 Soluble
11 G H 0.1178 Aggregation-prone
12 L H 0.4657 Aggregation-prone
13 V H 0.0000 Buried
14 Q H -1.4610 Soluble
15 P H -1.3690 Soluble
16 G H -1.4103 Soluble
17 G H -1.1152 Soluble
18 S H -1.1848 Soluble
19 L H -1.0865 Soluble
20 R H -1.8387 Soluble
21 L H 0.0000 Buried
22 S H -0.4513 Soluble
23 C H 0.0000 Buried
24 A H -0.1902 Soluble
25 A H 0.0000 Buried
26 S H -0.9265 Soluble
27 G H -1.0600 Soluble
28 F H -0.1471 Soluble
29 T H 0.0641 Aggregation-prone
30 F H 0.0000 Buried
35 S H -0.6331 Soluble
36 S H -0.1306 Soluble
37 Y H 0.5453 Aggregation-prone
38 G H -0.4389 Soluble
39 M H 0.0000 Buried
40 S H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.8097 Soluble
45 A H -1.1690 Soluble
46 P H -1.0172 Soluble
47 G H -1.4847 Soluble
48 K H -2.3566 Soluble
49 G H -1.5686 Soluble
50 L H 0.0000 Buried
51 E H -0.9662 Soluble
52 L H 0.0000 Buried
53 V H 0.0000 Buried
54 A H 0.0000 Buried
55 S H 0.0618 Aggregation-prone
56 I H 0.0000 Buried
57 N H -1.2892 Soluble
58 S H -1.1241 Soluble
59 N H -1.9437 Soluble
62 G H -1.7248 Soluble
63 G H -1.4036 Soluble
64 S H -0.9013 Soluble
65 T H -0.1581 Soluble
66 Y H 0.0618 Aggregation-prone
67 Y H -0.9134 Soluble
68 P H -1.6185 Soluble
69 D H -2.5575 Soluble
70 S H -1.8586 Soluble
71 V H 0.0000 Buried
72 K H -2.6526 Soluble
74 G H -1.8527 Soluble
75 R H -1.6098 Soluble
76 F H 0.0000 Buried
77 T H -0.8184 Soluble
78 I H 0.0000 Buried
79 S H -0.6412 Soluble
80 R H -1.3088 Soluble
81 D H -1.5590 Soluble
82 N H -1.7229 Soluble
83 A H -1.3193 Soluble
84 K H -2.1727 Soluble
85 N H -1.5703 Soluble
86 S H 0.0000 Buried
87 L H 0.0000 Buried
88 Y H -0.4846 Soluble
89 L H 0.0000 Buried
90 Q H -1.0832 Soluble
91 M H 0.0000 Buried
92 N H -1.4673 Soluble
93 S H -1.2848 Soluble
94 L H 0.0000 Buried
95 R H -2.0989 Soluble
96 A H -1.6192 Soluble
97 E H -2.1502 Soluble
98 D H 0.0000 Buried
99 T H -0.7038 Soluble
100 A H 0.0000 Buried
101 V H 0.1378 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 S H -0.0479 Soluble
107 G H -0.2942 Soluble
116 D H -0.5503 Soluble
117 Y H -0.1597 Soluble
118 W H -0.2905 Soluble
119 G H 0.0000 Buried
120 Q H -1.2918 Soluble
121 G H -0.5990 Soluble
122 T H -0.2643 Soluble
123 T H -0.0654 Soluble
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -0.6117 Soluble
128 S H -0.6738 Soluble
129 A H -0.4151 Soluble
130 S H -0.3768 Soluble
131 T H -0.7739 Soluble
132 K H -1.0738 Soluble
133 G H -1.3091 Soluble
134 P H 0.0000 Buried
135 S H -0.4745 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.0846 Soluble
139 L H 0.0000 Buried
140 A H -1.0888 Soluble
141 P H -0.6458 Soluble
142 G H -0.6296 Soluble
143 T H -0.4382 Soluble
144 A H -0.1933 Soluble
145 A H 0.0000 Buried
146 L H 0.0000 Buried
147 G H 0.0000 Buried
148 C H 0.0000 Buried
149 L H 0.0000 Buried
150 V H 0.0000 Buried
151 K H -0.3276 Soluble
152 D H -0.3913 Soluble
153 Y H 0.0000 Buried
154 F H 0.0000 Buried
155 P H -0.4974 Soluble
156 E H -0.7162 Soluble
157 P H -0.9076 Soluble
158 V H -0.5474 Soluble
159 T H -0.6145 Soluble
160 V H -0.3174 Soluble
161 S H -0.3841 Soluble
162 W H 0.0000 Buried
163 N H -0.7155 Soluble
164 S H -0.6853 Soluble
165 G H -0.5860 Soluble
166 A H -0.2659 Soluble
167 L H -0.0249 Soluble
168 T H -0.1895 Soluble
169 S H -0.1924 Soluble
170 G H -0.2352 Soluble
171 V H 0.1629 Aggregation-prone
172 H H -0.3269 Soluble
173 T H 0.0093 Aggregation-prone
174 F H 0.0000 Buried
175 P H -0.4124 Soluble
176 A H 0.2302 Aggregation-prone
177 V H 0.6036 Aggregation-prone
178 L H 1.4816 Aggregation-prone
179 Q H 0.4663 Aggregation-prone
180 S H 0.0263 Aggregation-prone
181 S H -0.1253 Soluble
182 G H 0.1284 Aggregation-prone
183 L H 0.1459 Aggregation-prone
184 Y H 0.4220 Aggregation-prone
185 S H 0.2637 Aggregation-prone
186 L H 0.0000 Buried
187 S H 0.0000 Buried
188 S H 0.0000 Buried
189 V H 0.0000 Buried
190 V H 0.0000 Buried
191 T H -0.1482 Soluble
192 V H 0.0000 Buried
193 P H -0.4521 Soluble
194 S H -0.4372 Soluble
195 S H -0.5628 Soluble
196 S H -0.5842 Soluble
197 L H -0.6109 Soluble
198 G H -0.9655 Soluble
199 T H -0.7879 Soluble
200 Q H -1.3916 Soluble
201 T H -1.1121 Soluble
202 Y H 0.0000 Buried
203 I H -1.1460 Soluble
204 C H 0.0000 Buried
205 N H -1.4383 Soluble
206 V H 0.0000 Buried
207 N H -1.9855 Soluble
208 H H 0.0000 Buried
209 K H -2.7852 Soluble
210 P H -1.6754 Soluble
211 S H -1.8104 Soluble
212 N H -2.6351 Soluble
213 T H -2.0709 Soluble
214 K H -2.7256 Soluble
215 V H -1.6714 Soluble
216 D H -2.4903 Soluble
217 K H -1.9904 Soluble
218 K H -2.2345 Soluble
219 V H 0.0000 Buried
220 E H -2.7223 Soluble
221 P H -1.9497 Soluble
222 K H -2.4274 Soluble
1 D L -1.1837 Soluble
2 I L 0.0000 Buried
3 V L 0.6665 Aggregation-prone
4 M L 0.0000 Buried
5 T L -0.3499 Soluble
6 Q L 0.0000 Buried
7 S L -0.4181 Soluble
8 P L -0.0725 Soluble
9 L L 0.3510 Aggregation-prone
10 S L -0.0322 Soluble
11 L L -0.1171 Soluble
12 P L -0.6437 Soluble
13 V L 0.0000 Buried
14 T L -1.4128 Soluble
15 P L -1.4774 Soluble
16 G L -1.6396 Soluble
17 E L -2.4971 Soluble
18 P L -2.1802 Soluble
19 A L 0.0000 Buried
20 S L -0.9934 Soluble
21 I L 0.0000 Buried
22 S L -0.9602 Soluble
23 C L 0.0000 Buried
24 R L -2.2581 Soluble
25 S L 0.0000 Buried
26 S L -0.9776 Soluble
27 Q L -1.5200 Soluble
28 S L -0.6248 Soluble
29 L L 0.0000 Buried
30 V L 0.9844 Aggregation-prone
31 Y L 0.8109 Aggregation-prone
32 S L -0.2782 Soluble
34 N L -1.2334 Soluble
35 G L -0.7337 Soluble
36 D L -0.5970 Soluble
37 T L 0.1138 Aggregation-prone
38 Y L 0.3179 Aggregation-prone
39 L L 0.0000 Buried
40 H L -0.2579 Soluble
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 L L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.7059 Soluble
46 P L -1.4384 Soluble
47 G L -1.3564 Soluble
48 Q L -2.0853 Soluble
49 S L -1.3332 Soluble
50 P L 0.0000 Buried
51 Q L -0.9840 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.2404 Soluble
56 K L -0.6696 Soluble
57 V L -0.5241 Soluble
65 S L -0.7938 Soluble
66 N L -1.1839 Soluble
67 R L -1.6229 Soluble
68 F L -0.6471 Soluble
69 S L -0.5680 Soluble
70 G L -0.7764 Soluble
71 V L -0.8323 Soluble
72 P L -1.2140 Soluble
74 D L -2.2931 Soluble
75 R L -1.9257 Soluble
76 F L 0.0000 Buried
77 S L -1.5611 Soluble
78 G L 0.0000 Buried
79 S L -0.9190 Soluble
80 G L -0.9894 Soluble
83 S L -0.6834 Soluble
84 G L -0.7067 Soluble
85 T L -1.4602 Soluble
86 D L -2.0160 Soluble
87 F L 0.0000 Buried
88 T L -1.1520 Soluble
89 L L 0.0000 Buried
90 K L -2.1792 Soluble
91 I L 0.0000 Buried
92 S L -2.1534 Soluble
93 R L -2.6801 Soluble
94 V L 0.0000 Buried
95 E L -1.7312 Soluble
96 A L -1.0869 Soluble
97 E L -1.9900 Soluble
98 D L 0.0000 Buried
99 V L -0.6819 Soluble
100 G L 0.0000 Buried
101 V L -0.3526 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 S L 0.0000 Buried
106 Q L 0.0000 Buried
107 S L 0.2593 Aggregation-prone
108 T L 0.6310 Aggregation-prone
109 H L 0.2880 Aggregation-prone
114 V L 1.2498 Aggregation-prone
115 P L 0.0000 Buried
116 W L 0.2863 Aggregation-prone
117 T L 0.2135 Aggregation-prone
118 F L 0.1574 Aggregation-prone
119 G L 0.0000 Buried
120 Q L -0.9597 Soluble
121 G L -0.6880 Soluble
122 T L 0.0000 Buried
123 K L -0.6660 Soluble
124 V L 0.0000 Buried
125 E L -0.7293 Soluble
126 I L -0.7978 Soluble
127 K L -1.3455 Soluble
128 R L -0.9499 Soluble
129 T L -0.0136 Soluble
130 V L 0.4853 Aggregation-prone
131 A L 0.0306 Aggregation-prone
132 A L -0.1586 Soluble
133 P L 0.0000 Buried
134 S L -0.1451 Soluble
135 V L 0.0000 Buried
136 F L 0.1484 Aggregation-prone
137 I L 0.0868 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.7190 Soluble
140 P L -0.8270 Soluble
141 S L -1.7602 Soluble
142 D L -2.8803 Soluble
143 E L -2.8161 Soluble
144 Q L 0.0000 Buried
145 L L -2.1516 Soluble
146 K L -2.8078 Soluble
147 S L -1.7315 Soluble
148 G L -1.2266 Soluble
149 T L -1.2026 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.9875 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.3412 Soluble
162 R L -2.2371 Soluble
163 E L -2.8273 Soluble
164 A L -2.1167 Soluble
165 K L -2.2093 Soluble
166 V L -1.1290 Soluble
167 Q L -0.7552 Soluble
168 W L 0.0000 Buried
169 K L -0.6260 Soluble
170 V L 0.0000 Buried
171 D L -1.8101 Soluble
172 N L -1.4769 Soluble
173 A L -0.2518 Soluble
174 L L 0.6900 Aggregation-prone
175 Q L -0.2142 Soluble
176 S L -0.4752 Soluble
177 G L -0.8487 Soluble
178 N L -0.7472 Soluble
179 S L -0.9369 Soluble
180 Q L -0.9575 Soluble
181 E L -1.4393 Soluble
182 S L -0.5264 Soluble
183 V L -0.4639 Soluble
184 T L -1.0599 Soluble
185 E L -2.2060 Soluble
186 Q L -1.9658 Soluble
187 D L -2.2432 Soluble
188 S L -1.8833 Soluble
189 K L -2.5061 Soluble
190 D L -2.1831 Soluble
191 S L -1.9696 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.4703 Soluble
199 L L 0.0000 Buried
200 T L -0.4118 Soluble
201 L L -0.6975 Soluble
202 S L -1.0701 Soluble
203 K L -2.0168 Soluble
204 A L -1.9248 Soluble
205 D L -2.6860 Soluble
206 Y L 0.0000 Buried
207 E L -3.4801 Soluble
208 K L -3.6080 Soluble
209 H L -3.0300 Soluble
210 K L -3.0434 Soluble
211 V L -1.4260 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.9369 Soluble
216 V L 0.0000 Buried
217 T L -1.1919 Soluble
218 H L 0.0000 Buried
219 Q L -1.5060 Soluble
220 G L -0.4611 Soluble
221 L L -0.2717 Soluble
222 S L -0.4704 Soluble
223 S L -0.4009 Soluble
224 P L -0.4897 Soluble
225 V L 0.0243 Aggregation-prone
226 T L -0.5083 Soluble
227 K L -0.7654 Soluble
228 S L -0.6223 Soluble
229 F L -0.9790 Soluble
230 N L -1.9011 Soluble
231 R L -2.5756 Soluble
232 G L -2.1528 Soluble
233 E L -2.5540 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6696 1.8128 CSV PDB
4.5 -0.7157 1.7701 CSV PDB
5.0 -0.7691 1.7149 CSV PDB
5.5 -0.8201 1.656 CSV PDB
6.0 -0.8572 1.6038 CSV PDB
6.5 -0.8716 1.5664 CSV PDB
7.0 -0.863 1.5462 CSV PDB
7.5 -0.8388 1.5398 CSV PDB
8.0 -0.8053 1.541 CSV PDB
8.5 -0.7642 1.5452 CSV PDB
9.0 -0.7157 1.6191 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH