Cugrastomig / 7um3

A4DAB077_7um3_H_L | Source PDB: 7um3 | Chains: H, L | Done
97.36
Yes
10 Å
Yes
Avg A4D Score
-0.691
Max Score
1.7564
Min Score
-3.7138
Total Score
-295.08
Score CDR
-0.3085
Score non-CDR
-0.7361

Calculation Parameters

Chain Sequence(s) VQLQQWGAGLLKPSETLSLTCAVYGFSDYYWNWIRQPPGKGLEWIGEINHNGNTNSNPSLKSRVTLSLDTSKNQFSLKLRSVTAADTAVYYCAFGYSDYEYNWFDPWGQGTLVTVSSASTKGPSVFPLAPSSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSEIVLTQSPATLSLSPGERATLSCRASQSISSYLAWYQQKPGQAPRLLIYDASNRATGIPARFSGSGSGTDFTLTISSLEPEDFAVYYCQQRSNWPLTFGQGTNLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.7138
Maximum Score 1.7564
Average Score -0.691
Total Score -295.0771

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V H 1.1969 Aggregation-prone
3 Q H -0.3036 Soluble
4 L H -0.4670 Soluble
5 Q H -1.1528 Soluble
6 Q H -0.6003 Soluble
7 W H 0.0130 Aggregation-prone
8 G H 0.1619 Aggregation-prone
9 A H 0.2937 Aggregation-prone
11 G H 0.5171 Aggregation-prone
12 L H 0.5045 Aggregation-prone
13 L H 0.0000 Buried
14 K H -1.5513 Soluble
15 P H -1.3097 Soluble
16 S H -1.3756 Soluble
17 E H -1.7981 Soluble
18 T H -1.4322 Soluble
19 L H 0.0000 Buried
20 S H -0.6732 Soluble
21 L H 0.0000 Buried
22 T H -0.2731 Soluble
23 C H 0.0000 Buried
24 A H 0.0000 Buried
25 V H -0.1612 Soluble
26 Y H 0.3256 Aggregation-prone
27 G H 0.6365 Aggregation-prone
28 F H 1.7564 Aggregation-prone
29 S H 0.4613 Aggregation-prone
36 D H -0.2832 Soluble
37 Y H 0.1782 Aggregation-prone
38 Y H -0.1533 Soluble
39 W H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 I H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.7566 Soluble
45 P H -0.8579 Soluble
46 P H -0.9693 Soluble
47 G H -1.5264 Soluble
48 K H -2.3762 Soluble
49 G H -1.5730 Soluble
50 L H 0.0000 Buried
51 E H -0.9225 Soluble
52 W H 0.0000 Buried
53 I H 0.0000 Buried
54 G H 0.0000 Buried
55 E H 0.0000 Buried
56 I H 0.0000 Buried
57 N H 0.0000 Buried
58 H H -1.1528 Soluble
59 N H -1.9535 Soluble
63 G H -1.5554 Soluble
64 N H -1.4993 Soluble
65 T H -0.9343 Soluble
66 N H -0.6420 Soluble
67 S H -0.6184 Soluble
68 N H 0.0000 Buried
69 P H -1.3049 Soluble
70 S H -1.0501 Soluble
71 L H 0.0000 Buried
72 K H -2.0215 Soluble
74 S H -1.3436 Soluble
75 R H -1.3735 Soluble
76 V H -0.8970 Soluble
77 T H -0.5144 Soluble
78 L H -0.1456 Soluble
79 S H -0.3323 Soluble
80 L H -0.6378 Soluble
81 D H -1.3856 Soluble
82 T H -1.2436 Soluble
83 S H -1.4700 Soluble
84 K H -2.2588 Soluble
85 N H -1.6476 Soluble
86 Q H -1.3733 Soluble
87 F H 0.0000 Buried
88 S H -0.4175 Soluble
89 L H 0.0000 Buried
90 K H -1.1869 Soluble
91 L H 0.0000 Buried
92 R H -1.6019 Soluble
93 S H -1.3769 Soluble
94 V H 0.0000 Buried
95 T H -0.6570 Soluble
96 A H -0.2042 Soluble
97 A H 0.0400 Aggregation-prone
98 D H 0.0000 Buried
99 T H 0.1703 Aggregation-prone
100 A H 0.0000 Buried
101 V H 0.2387 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 F H 0.0000 Buried
107 G H 0.0000 Buried
108 Y H 0.0000 Buried
109 S H 0.4643 Aggregation-prone
110 D H 0.3767 Aggregation-prone
111 Y H 0.8045 Aggregation-prone
112A E H -0.7982 Soluble
112 Y H 0.5390 Aggregation-prone
113 N H -0.3173 Soluble
114 W H -0.0682 Soluble
115 F H 0.4668 Aggregation-prone
116 D H 0.0000 Buried
117 P H -0.3338 Soluble
118 W H -0.8707 Soluble
119 G H 0.0000 Buried
120 Q H -1.5251 Soluble
121 G H -0.7035 Soluble
122 T H 0.0640 Aggregation-prone
123 L H 0.4131 Aggregation-prone
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -0.4712 Soluble
128 S H -0.7438 Soluble
129 A H -0.5753 Soluble
130 S H -0.5782 Soluble
131 T H -0.6938 Soluble
132 K H -1.2083 Soluble
133 G H -1.4075 Soluble
134 P H -0.6398 Soluble
135 S H -0.2243 Soluble
136 V H 0.0000 Buried
137 F H -0.7464 Soluble
138 P H -1.3095 Soluble
139 L H 0.0000 Buried
140 A H -1.2186 Soluble
141 P H 0.0000 Buried
142 S H -0.9058 Soluble
143 S H -0.5810 Soluble
144 G H -0.6644 Soluble
145 T H -0.4966 Soluble
146 A H 0.0000 Buried
147 A H 0.0000 Buried
148 L H 0.0000 Buried
149 G H 0.0000 Buried
150 C H 0.0000 Buried
151 L H 0.0000 Buried
152 V H 0.0000 Buried
153 K H 0.0000 Buried
154 D H -0.4573 Soluble
155 Y H 0.0000 Buried
156 F H 0.0000 Buried
157 P H 0.0000 Buried
158 E H -0.4496 Soluble
159 P H -0.7782 Soluble
160 V H -0.7779 Soluble
161 T H -0.6478 Soluble
162 V H -0.1839 Soluble
163 S H -0.3732 Soluble
164 W H 0.0000 Buried
165 N H -0.7243 Soluble
166 S H -0.6229 Soluble
167 G H -0.5002 Soluble
168 A H -0.2297 Soluble
169 L H 0.0313 Aggregation-prone
170 T H -0.1459 Soluble
171 S H -0.1631 Soluble
172 G H -0.2182 Soluble
173 V H 0.2297 Aggregation-prone
174 H H 0.0000 Buried
175 T H -0.1346 Soluble
176 F H 0.0000 Buried
177 P H -0.5413 Soluble
178 A H -0.0871 Soluble
179 V H 0.1520 Aggregation-prone
180 L H 0.7670 Aggregation-prone
181 Q H -0.0251 Soluble
182 S H -0.2502 Soluble
183 S H -0.2500 Soluble
184 G H -0.0694 Soluble
185 L H -0.0527 Soluble
186 Y H 0.0000 Buried
187 S H 0.0000 Buried
188 L H 0.0000 Buried
189 S H 0.0000 Buried
190 S H 0.0000 Buried
191 V H 0.0000 Buried
192 V H 0.0000 Buried
193 T H -0.1203 Soluble
194 V H 0.0000 Buried
195 P H -0.6012 Soluble
196 S H -0.6009 Soluble
197 S H -0.6545 Soluble
198 S H -0.6953 Soluble
199 L H -0.9309 Soluble
200 G H -1.0298 Soluble
201 T H -0.8153 Soluble
202 Q H -1.3590 Soluble
203 T H -1.1616 Soluble
204 Y H 0.0000 Buried
205 I H -1.2903 Soluble
206 C H 0.0000 Buried
207 N H -1.5514 Soluble
208 V H 0.0000 Buried
209 N H -2.1992 Soluble
210 H H 0.0000 Buried
211 K H -2.7885 Soluble
212 P H -1.5267 Soluble
213 S H -1.8531 Soluble
214 N H -2.6374 Soluble
215 T H -2.1593 Soluble
216 K H -2.8654 Soluble
217 V H -1.7831 Soluble
218 D H -2.5284 Soluble
219 K H -2.2941 Soluble
220 R H -2.6628 Soluble
221 V H 0.0000 Buried
222 E H -2.9522 Soluble
223 P H -2.0378 Soluble
224 K H -2.7837 Soluble
225 S H -1.6082 Soluble
1 E L -1.7554 Soluble
2 I L 0.0000 Buried
3 V L 0.3098 Aggregation-prone
4 L L 0.0000 Buried
5 T L -0.8199 Soluble
6 Q L 0.0000 Buried
7 S L -0.7046 Soluble
8 P L -0.2877 Soluble
9 A L -0.4145 Soluble
10 T L -0.2727 Soluble
11 L L -0.1426 Soluble
12 S L -0.5148 Soluble
13 L L -1.0019 Soluble
14 S L -1.3937 Soluble
15 P L -1.7314 Soluble
16 G L -1.8416 Soluble
17 E L -2.4928 Soluble
18 R L -2.6470 Soluble
19 A L 0.0000 Buried
20 T L -0.6618 Soluble
21 L L 0.0000 Buried
22 S L -0.9358 Soluble
23 C L 0.0000 Buried
24 R L -2.5395 Soluble
25 A L 0.0000 Buried
26 S L -1.2434 Soluble
27 Q L -1.8755 Soluble
28 S L -1.3221 Soluble
29 I L 0.0000 Buried
36 S L -0.6209 Soluble
37 S L -0.3943 Soluble
38 Y L 0.2689 Aggregation-prone
39 L L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.6415 Soluble
46 P L -1.4212 Soluble
47 G L -1.4324 Soluble
48 Q L -2.1566 Soluble
49 A L -1.5721 Soluble
50 P L 0.0000 Buried
51 R L -2.0368 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.6451 Soluble
56 D L -1.0207 Soluble
57 A L 0.0000 Buried
65 S L -1.0814 Soluble
66 N L -1.5434 Soluble
67 R L -1.5571 Soluble
68 A L -0.9128 Soluble
69 T L -0.5885 Soluble
70 G L -0.5944 Soluble
71 I L -0.4989 Soluble
72 P L -0.3891 Soluble
74 A L -0.3695 Soluble
75 R L -0.6767 Soluble
76 F L 0.0000 Buried
77 S L -0.6897 Soluble
78 G L 0.0000 Buried
79 S L -0.8994 Soluble
80 G L -1.1713 Soluble
83 S L -0.8909 Soluble
84 G L -1.2295 Soluble
85 T L -2.0775 Soluble
86 D L -2.8514 Soluble
87 F L 0.0000 Buried
88 T L -0.8791 Soluble
89 L L 0.0000 Buried
90 T L -0.6148 Soluble
91 I L 0.0000 Buried
92 S L -1.3901 Soluble
93 S L -1.7494 Soluble
94 L L 0.0000 Buried
95 E L -1.9488 Soluble
96 P L -1.5813 Soluble
97 E L -2.2432 Soluble
98 D L 0.0000 Buried
99 F L -0.6121 Soluble
100 A L 0.0000 Buried
101 V L -0.2903 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 R L -0.3502 Soluble
108 S L -0.6567 Soluble
109 N L -1.3805 Soluble
114 W L -0.8654 Soluble
115 P L -0.8908 Soluble
116 L L 0.0000 Buried
117 T L -0.3261 Soluble
118 F L -0.0271 Soluble
119 G L 0.0000 Buried
120 Q L -1.4389 Soluble
121 G L -1.0052 Soluble
122 T L 0.0000 Buried
123 N L -0.7638 Soluble
124 L L 0.0000 Buried
125 E L 0.0000 Buried
126 I L -0.7022 Soluble
127 K L -1.4585 Soluble
128 R L -1.0604 Soluble
129 T L -0.1384 Soluble
130 V L 0.3049 Aggregation-prone
131 A L 0.1060 Aggregation-prone
132 A L -0.0723 Soluble
133 P L 0.0000 Buried
134 S L -0.0012 Soluble
135 V L 0.1573 Aggregation-prone
136 F L 0.3118 Aggregation-prone
137 I L 0.1923 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.5504 Soluble
140 P L 0.0000 Buried
141 S L 0.0000 Buried
142 D L -3.0350 Soluble
143 E L -3.1164 Soluble
144 Q L 0.0000 Buried
145 L L -2.1552 Soluble
146 K L -2.8617 Soluble
147 S L -1.7908 Soluble
148 G L -1.4062 Soluble
149 T L -1.0844 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.6729 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.6316 Soluble
162 R L -2.7664 Soluble
163 E L -3.0648 Soluble
164 A L -2.2035 Soluble
165 K L -2.1833 Soluble
166 V L -1.1415 Soluble
167 Q L -0.7140 Soluble
168 W L 0.0000 Buried
169 K L -0.8052 Soluble
170 V L 0.0000 Buried
171 D L -2.1529 Soluble
172 N L -1.6733 Soluble
173 A L -0.4591 Soluble
174 L L 0.3462 Aggregation-prone
175 Q L -0.4779 Soluble
176 S L -0.8386 Soluble
177 G L -1.2986 Soluble
178 N L -1.6659 Soluble
179 S L -1.5810 Soluble
180 Q L -1.6200 Soluble
181 E L -2.0028 Soluble
182 S L -0.9236 Soluble
183 V L -0.8946 Soluble
184 T L -1.1676 Soluble
185 E L -2.3051 Soluble
186 Q L -1.7972 Soluble
187 D L -1.8307 Soluble
188 S L -1.6690 Soluble
189 K L -2.1817 Soluble
190 D L -1.5719 Soluble
191 S L -1.6156 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.7521 Soluble
199 L L 0.0000 Buried
200 T L -0.7149 Soluble
201 L L -0.9671 Soluble
202 S L -1.1712 Soluble
203 K L -2.0872 Soluble
204 A L -1.9371 Soluble
205 D L -2.8596 Soluble
206 Y L 0.0000 Buried
207 E L -3.5251 Soluble
208 K L -3.7138 Soluble
209 H L -3.2692 Soluble
210 K L -3.3758 Soluble
211 V L -1.8657 Soluble
212 Y L 0.0000 Buried
213 A L -1.0494 Soluble
214 C L 0.0000 Buried
215 E L -0.8102 Soluble
216 V L 0.0000 Buried
217 T L -1.1473 Soluble
218 H L 0.0000 Buried
219 Q L -1.5288 Soluble
220 G L -0.4123 Soluble
221 L L -0.2375 Soluble
222 S L -0.4445 Soluble
223 S L -0.4337 Soluble
224 P L -0.5575 Soluble
225 V L -0.1091 Soluble
226 T L -0.5454 Soluble
227 K L -0.7612 Soluble
228 S L -0.7984 Soluble
229 F L 0.0000 Buried
230 N L -2.5308 Soluble
231 R L -2.8093 Soluble
232 G L -2.3201 Soluble
233 E L -2.6530 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
Load structure to view sequence…
Downloads
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6871 3.7373 CSV PDB
4.5 -0.7298 3.6891 CSV PDB
5.0 -0.7799 3.6398 CSV PDB
5.5 -0.8275 3.592 CSV PDB
6.0 -0.8611 3.5482 CSV PDB
6.5 -0.8715 3.5079 CSV PDB
7.0 -0.8582 3.4679 CSV PDB
7.5 -0.8294 3.4268 CSV PDB
8.0 -0.7922 3.3895 CSV PDB
8.5 -0.7491 3.3622 CSV PDB
9.0 -0.7004 3.3465 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH